Restriction map for
pFN21AE3435
(Circular) (Six-base) MAPSORT of: pFN21AE3435.seq Check: 7574 from: 1 to: 7486
pFN21AE3435 7486 bp
With 182 enzymes: SgfI *
March 28, 2012 12:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2461 3572 4262 2461
Size: 1111 690 5685
Fragments arranged by size:
5685 1111 690
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5296 278
Size: 53 83 186 936 3760 2468
Fragments arranged by size:
3760 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4829 1937
Size: 2892 4594
Acc65I G'GTAC_C
Cuts at: 4807 4807
Size: 7486
AclI AA'CG_TT
Cuts at: 1563 4785 5613 5986 1563
Size: 3222 828 373 3063
Fragments arranged by size:
3222 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2453 2545 3033 5549 6933 7201 1632
Size: 821 92 488 2516 1384 268 1917
Fragments arranged by size:
2516 1917 1384 821 488 268 92
AflII C'TTAA_G
Cuts at: 820 1017 4306 820
Size: 197 3289 4000
Fragments arranged by size:
4000 3289 197
AflIII A'CryG_T
Cuts at: 1203 2734 3149 6385 7362 1203
Size: 1531 415 3236 977 1327
Fragments arranged by size:
3236 1531 1327 977 415
AgeI A'CCGG_T
Cuts at: 4401 6317 4401
Size: 1916 5570
AhdI GACnn_n'nnGTC
Cuts at: 6215 6215
Size: 7486
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7486
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3106 1184
Size: 1922 5564
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2326 2358 2326
Size: 32 7454
AlwNI CAG_nnn'CTG
Cuts at: 2279 2453 2942 3225 5229 6801 2279
Size: 174 489 283 2004 1572 2964
Fragments arranged by size:
2964 2004 1572 489 283 174
ApaLI G'TGCA_C
Cuts at: 2283 2730 5543 6699 2283
Size: 447 2813 1156 3070
Fragments arranged by size:
3070 2813 1156 447
ApoI r'AATT_y
Cuts at: 1493 2654 3285 3492 3625 3723 3768 4068 4795
Size: 1161 631 207 133 98 45 300 727
Cuts at: 4795 5021 5173 7282 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1161 727 631 300 226 207
152 133 98 45
AseI AT'TA_AT
Cuts at: 160 1032 2667 6040 160
Size: 872 1635 3373 1606
Fragments arranged by size:
3373 1635 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7486
AvaI C'yCGr_G
Cuts at: 1870 1943 4811 1870
Size: 73 2868 4545
Fragments arranged by size:
4545 2868 73
BamHI G'GATC_C
Cuts at: 4816 5183 4816
Size: 367 7119
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2366 4807 6262 618
Size: 325 217 627 579 2441 1455 1842
Fragments arranged by size:
2441 1842 1455 627 579 325 217
BanII G_rGCy'C
Cuts at: 1391 4805 1391
Size: 3414 4072
BbeI G_GCGC'C
Cuts at: 2370 2370
Size: 7486
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3972 4110 928
Size: 971 2073 138 4304
Fragments arranged by size:
4304 2073 971 138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5677 5711 1903
Size: 34 3740 34 3678
Fragments arranged by size:
3740 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3900 5378 6588 3900
Size: 1478 1210 4798
Fragments arranged by size:
4798 1478 1210
BclI T'GATC_A
Cuts at: 1548 3127 3391 1548
Size: 1579 264 5643
Fragments arranged by size:
5643 1579 264
BfrBI ATG'CAT
Cuts at: 2638 3713 4546 2638
Size: 1075 833 5578
Fragments arranged by size:
5578 1075 833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6097 136
Size: 107 122 71 631 605 4425 1525
Fragments arranged by size:
4425 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2437 7481 2437
Size: 5044 2442
BlpI GC'TnA_GC
Cuts at: 4903 4903
Size: 7486
Bme1580I G_kGCm'C
Cuts at: 1790 2287 2734 5547 6703 1790
Size: 497 447 2813 1156 2573
Fragments arranged by size:
2813 2573 1156 497 447
BmgBI CAC'GTC
Cuts at: 1318 3368 1318
Size: 2050 5436
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5217 6175 442
Size: 416 53 83 399 24 3800 958 1753
Fragments arranged by size:
3800 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7486
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 7486
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3531 3870 3879 4155 6146 1239
Size: 2292 339 9 276 1991 2579
Fragments arranged by size:
2579 2292 1991 339 276 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4251 5611 6476 6774 4251
Size: 1360 865 298 4963
Fragments arranged by size:
4963 1360 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2026 4238 6149 882
Size: 617 527 2212 1911 2219
Fragments arranged by size:
2219 2212 1911 617 527
BsaAI yAC'GTr
Cuts at: 493 1204 5277 493
Size: 711 4073 2702
Fragments arranged by size:
4073 2702 711
BsaBI GATnn'nnATC
Cuts at: 4158 7480 4158
Size: 3322 4164
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 5293 5675 6310
Size: 53 83 186 936 834 2926 382 635
Cuts at: 6310 275
Size: 1451
Fragments arranged by size:
2926 1451 936 834 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2028 3903 4401 5919 6317 6591 6738 7149 2028
Size: 1875 498 1518 398 274 147 411 2365
Fragments arranged by size:
2365 1875 1518 498 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3852 3882 1178
Size: 30 165 30 2449 30 4782
Fragments arranged by size:
4782 2449 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3666 1186
Size: 2480 5006
BseYI C'CCAG_C
Cuts at: 2112 2127 3305 6689 7185 2112
Size: 15 1178 3384 496 2413
Fragments arranged by size:
3384 2413 1178 496 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2084 2955 3051 1163
Size: 573 348 871 96 5598
Fragments arranged by size:
5598 871 573 348 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2054 4936 5697 6725 1540
Size: 275 186 53 2882 761 1028 2301
Fragments arranged by size:
2882 2301 1028 761 275 186 53
BsiHKAI G_wGCw'C
Cuts at: 2287 2734 5547 5632 6703 2287
Size: 447 2813 85 1071 3070
Fragments arranged by size:
3070 2813 1071 447 85
BsmI GAATG_Cn'
Cuts at: 4267 4997 5090 4267
Size: 730 93 6663
Fragments arranged by size:
6663 730 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2287 2734 4805 5547 5632 6703 1391
Size: 399 497 447 2071 742 85 1071 2174
Fragments arranged by size:
2174 2071 1071 742 497 447 399 85
BspEI T'CCGG_A
Cuts at: 7149 7149
Size: 7486
BspHI T'CATG_A
Cuts at: 3772 5375 7105 3772
Size: 1603 1730 4153
Fragments arranged by size:
4153 1730 1603
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2461 3572 3913 4262 4331 4841 844
Size: 1617 1111 341 349 69 510 3489
Fragments arranged by size:
3489 1617 1111 510 349 341 69
BsrBI CCG'CTC
Cuts at: 1394 1823 2215 2233 5373 1394
Size: 429 392 18 3140 3507
Fragments arranged by size:
3507 3140 429 392 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 3715 4134 4772 5981 6155 65
Size: 1183 2467 419 638 1209 174 1396
Fragments arranged by size:
2467 1396 1209 1183 638 419 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4401 6130 6317 1700
Size: 110 2591 1729 187 2869
Fragments arranged by size:
2869 2591 1729 187 110
BsrGI T'GTAC_A
Cuts at: 96 3152 4700 96
Size: 3056 1548 2882
Fragments arranged by size:
3056 2882 1548
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7486
BssSI C'ACGA_G
Cuts at: 5546 6558 5546
Size: 1012 6474
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2411 2453 794
Size: 1617 42 5827
Fragments arranged by size:
5827 1617 42
BstBI TT'CG_AA
Cuts at: 6290 6290
Size: 7486
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7486
BstXI CCAn_nnnn'nTGG
Cuts at: 2589 2697 3788 2589
Size: 108 1091 6287
Fragments arranged by size:
6287 1091 108
BstYI r'GATC_y
Cuts at: 1972 2437 2527 4816 5183 5568 5585 7037 7123
Size: 465 90 2289 367 385 17 1452 86
Cuts at: 7123 7135 7481 1972
Size: 12 346 1977
Fragments arranged by size:
2289 1977 1452 465 385 367 346 90
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2208 2208
Size: 7486
BtgI C'CryG_G
Cuts at: 513 1065 3499 513
Size: 552 2434 4500
Fragments arranged by size:
4500 2434 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6747
Fragments arranged by size:
6747 683 56
BtsI GCAGTG_nn'
Cuts at: 3088 5002 5796 5816 3088
Size: 1914 794 20 4758
Fragments arranged by size:
4758 1914 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7486
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7451
DraI TTT'AAA
Cuts at: 4790 4790
Size: 7486
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6493 809
Size: 507 5177 1802
Fragments arranged by size:
5177 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2051 3778 4933
Size: 54 1425 50 275 18 221 1727 1155
Cuts at: 4933 5822 7451 8
Size: 889 1629 43
Fragments arranged by size:
1727 1629 1425 1155 889 275 221 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2051 4933 1537
Size: 275 239 2882 4090
Fragments arranged by size:
4090 2882 275 239
EarI CTCTTCn'nnn_
Cuts at: 1353 2670 2785 3532 3822 4189 5416 1353
Size: 1317 115 747 290 367 1227 3423
Fragments arranged by size:
3423 1317 1227 747 367 290 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2130 2166 2187 6073 6447 6593
Size: 1064 138 541 36 21 3886 374 146
Cuts at: 6593 387
Size: 1280
Fragments arranged by size:
3886 1280 1064 541 374 146 138 36
21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 2545 3033 3531 3870 3879 4155
Size: 393 821 92 488 498 339 9 276
Cuts at: 4155 5549 6146 6933 7201 1239
Size: 1394 597 787 268 1524
Fragments arranged by size:
1524 1394 821 787 597 498 488 393
339 276 268 92 9
EcoO109I rG'GnC_Cy
Cuts at: 2276 2276
Size: 7486
EcoRI G'AATT_C
Cuts at: 3723 4068 4795 5173 3723
Size: 345 727 378 6036
Fragments arranged by size:
6036 727 378 345
EcoRV GAT'ATC
Cuts at: 3976 3976
Size: 7486
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2846 2846
Size: 7486
FspI TGC'GCA
Cuts at: 5992 5992
Size: 7486
HaeII r_GCGC'y
Cuts at: 1678 2370 6633 1678
Size: 692 4263 2531
Fragments arranged by size:
4263 2531 692
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3047 3079 3645 3677 6127
Size: 32 135 32 1475 32 566 32 2450
Cuts at: 6127 6159 6201 6233 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2450 1475 566 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4830 5076 669
Size: 988 281 2892 246 3079
Fragments arranged by size:
3079 2892 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7486
HpaI GTT'AAC
Cuts at: 5076 5076
Size: 7486
KasI G'GCGC_C
Cuts at: 2366 2366
Size: 7486
KpnI G_GTAC'C
Cuts at: 4811 4811
Size: 7486
MfeI C'AATT_G
Cuts at: 3750 5085 3750
Size: 1335 6151
MluI A'CGCG_T
Cuts at: 7362 7362
Size: 7486
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3007 3217 6600 6784 1305
Size: 1702 210 3383 184 2007
Fragments arranged by size:
3383 2007 1702 210 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3780 10
Size: 54 1425 343 1948 3716
Fragments arranged by size:
3716 1948 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2856 3078 3366 4598 5444 5803 5962
Size: 648 1690 222 288 1232 846 359 159
Cuts at: 5962 518
Size: 2042
Fragments arranged by size:
2042 1690 1232 846 648 359 288 222
159
MspA1I CmG'CkG
Cuts at: 1606 2203 4902 5579 6727 6972 1606
Size: 597 2699 677 1148 245 2120
Fragments arranged by size:
2699 2120 1148 677 597 245
NarI GG'CG_CC
Cuts at: 2367 2367
Size: 7486
NcoI C'CATG_G
Cuts at: 513 1065 3499 513
Size: 552 2434 4500
Fragments arranged by size:
4500 2434 552
NdeI CA'TA_TG
Cuts at: 387 3987 4595 387
Size: 3600 608 3278
Fragments arranged by size:
3600 3278 608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7486
NotI GC'GGCC_GC
Cuts at: 2051 4933 2051
Size: 2882 4604
NsiI A_TGCA'T
Cuts at: 2640 3715 4548 2640
Size: 1075 833 5578
Fragments arranged by size:
5578 1075 833
NspI r_CATG'y
Cuts at: 1132 1229 2738 3153 3195 4844 6389 1132
Size: 97 1509 415 42 1649 1545 2229
Fragments arranged by size:
2229 1649 1545 1509 415 97 42
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7486
PciI A'CATG_T
Cuts at: 2734 3149 6385 2734
Size: 415 3236 3835
Fragments arranged by size:
3835 3236 415
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7486
PmeI GTTT'AAAC
Cuts at: 4790 4790
Size: 7486
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5555 5587 7068 7100 5555
Size: 32 1481 32 5941
Fragments arranged by size:
5941 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2276 2276
Size: 7486
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7486
PsiI TTA'TAA
Cuts at: 2966 4080 5056 2966
Size: 1114 976 5396
Fragments arranged by size:
5396 1114 976
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3145 3177 3145
Size: 32 7454
PstI C_TGCA'G
Cuts at: 830 2254 3169 4838 830
Size: 1424 915 1669 3478
Fragments arranged by size:
3478 1669 1424 915
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7486
SalI G'TCGA_C
Cuts at: 1936 4828 1936
Size: 2892 4594
SbfI CC_TGCA'GG
Cuts at: 4838 4838
Size: 7486
ScaI AGT'ACT
Cuts at: 3891 5734 7446 3891
Size: 1843 1712 3931
Fragments arranged by size:
3931 1843 1712
SfcI C'TryA_G
Cuts at: 826 1045 2250 2382 2764 3165 3474 4555 4758
Size: 219 1205 132 382 401 309 1081 203
Cuts at: 4758 4834 5969 6650 6841 826
Size: 76 1135 681 191 1471
Fragments arranged by size:
1471 1205 1135 1081 681 401 382 309
219 203 191 132 76
SfoI GGC'GCC
Cuts at: 2368 2368
Size: 7486
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7486
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7486
SmaI CCC'GGG
Cuts at: 1872 4813 1872
Size: 2941 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4266 4306 5590 6491 6753 820
Size: 197 926 2323 40 1284 901 262 1553
Fragments arranged by size:
2323 1553 1284 926 901 262 197 40
SnaBI TAC'GTA
Cuts at: 493 5277 493
Size: 4784 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7486
SphI G_CATG'C
Cuts at: 1132 4844 1132
Size: 3712 3774
SspI AAT'ATT
Cuts at: 5 52 4116 4323 5410 5
Size: 47 4064 207 1087 2081
Fragments arranged by size:
4064 2081 1087 207 47
StuI AGG'CCT
Cuts at: 1517 3681 1517
Size: 2164 5322
StyI C'CwwG_G
Cuts at: 513 1065 2830 3499 4925 513
Size: 552 1765 669 1426 3074
Fragments arranged by size:
3074 1765 1426 669 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3856 5513 5530 5683 5868 6313 325
Size: 1255 2276 1657 17 153 185 445 1498
Fragments arranged by size:
2276 1657 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3146 3152 3889
Size: 274 80 33 51 1443 1169 6 737
Cuts at: 3889 4700 5732 7444 96
Size: 811 1032 1712 138
Fragments arranged by size:
1712 1443 1169 1032 811 737 274 138
80 51 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3852 3884 3898 3930 3942 3974 7446 7478 3852
Size: 32 14 32 12 32 3472 32 3860
Fragments arranged by size:
3860 3472 32 32 32 32 14 12
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7486
XbaI T'CTAG_A
Cuts at: 4822 4822
Size: 7486
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3506 3506
Size: 7486
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7486
XmaI C'CCGG_G
Cuts at: 1870 4811 1870
Size: 2941 4545
XmnI GAAnn'nnTTC
Cuts at: 2493 5615 2493
Size: 3122 4364
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5294 276
Size: 53 83 186 936 3760 2468
Fragments arranged by size:
3760 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI FseI FspAI NaeI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI