Restriction map for
pFN21AE3428
(Circular) (Six-base) MAPSORT of: pFN21AE3428.seq Check: 6866 from: 1 to: 6511
pFN21AE3428 6511 bp
With 182 enzymes: SgfI *
March 28, 2012 12:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4321 278
Size: 53 83 186 936 2785 2468
Fragments arranged by size:
2785 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2311 3854 1937
Size: 374 1543 4594
Fragments arranged by size:
4594 1543 374
Acc65I G'GTAC_C
Cuts at: 3661 3832 3661
Size: 171 6340
AclI AA'CG_TT
Cuts at: 1563 4638 5011 1563
Size: 3075 373 3063
Fragments arranged by size:
3075 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2603 2754 3318 3534 3731 4574 5958 6226
Size: 971 151 564 216 197 843 1384 268
Cuts at: 6226 1632
Size: 1917
Fragments arranged by size:
1917 1384 971 843 564 268 216 197
151
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6314
AflIII A'CryG_T
Cuts at: 1203 5410 6387 1203
Size: 4207 977 1327
Fragments arranged by size:
4207 1327 977
AgeI A'CCGG_T
Cuts at: 5342 5342
Size: 6511
AhdI GACnn_n'nnGTC
Cuts at: 2866 5240 2866
Size: 2374 4137
AleI CACnn'nnGTG
Cuts at: 1166 3423 1166
Size: 2257 4254
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6511
AlwNI CAG_nnn'CTG
Cuts at: 2777 3749 3779 4254 5826 2777
Size: 972 30 475 1572 3462
Fragments arranged by size:
3462 1572 972 475 30
ApaLI G'TGCA_C
Cuts at: 4568 5724 4568
Size: 1156 5355
ApoI r'AATT_y
Cuts at: 1493 2767 3125 3243 3651 3820 4046 4198 6307
Size: 1274 358 118 408 169 226 152 2109
Cuts at: 6307 1493
Size: 1697
Fragments arranged by size:
2109 1697 1274 408 358 226 169 152
118
AseI AT'TA_AT
Cuts at: 160 1032 2483 5065 160
Size: 872 1451 2582 1606
Fragments arranged by size:
2582 1606 1451 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
AvaI C'yCGr_G
Cuts at: 1870 1943 2985 3836 1870
Size: 73 1042 851 4545
Fragments arranged by size:
4545 1042 851 73
BamHI G'GATC_C
Cuts at: 3841 4208 3841
Size: 367 6144
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2133 3661 3832 5287 618
Size: 325 217 627 346 1528 171 1455 1842
Fragments arranged by size:
1842 1528 1455 627 346 325 217 171
BanII G_rGCy'C
Cuts at: 1391 2084 3070 3830 1391
Size: 693 986 760 4072
Fragments arranged by size:
4072 986 760 693
BbeI G_GCGC'C
Cuts at: 2137 2137
Size: 6511
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2229 3719 928
Size: 971 330 1490 3720
Fragments arranged by size:
3720 1490 971 330
BbvCI CC'TCA_GC
Cuts at: 3249 3249
Size: 6511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4702 4736 1903
Size: 34 2765 34 3678
Fragments arranged by size:
3678 2765 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2600 4403 5613 2600
Size: 1803 1210 3498
Fragments arranged by size:
3498 1803 1210
BclI T'GATC_A
Cuts at: 1548 3580 1548
Size: 2032 4479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3059 5122 136
Size: 107 122 71 631 605 1387 2063 1525
Fragments arranged by size:
2063 1525 1387 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2434 2701 3698 6506 2434
Size: 267 997 2808 2439
Fragments arranged by size:
2808 2439 997 267
BlpI GC'TnA_GC
Cuts at: 3928 3928
Size: 6511
Bme1580I G_kGCm'C
Cuts at: 1790 4572 5728 1790
Size: 2782 1156 2573
Fragments arranged by size:
2782 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6511
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3759 4242 5200
Size: 416 53 83 399 24 2342 483 958
Cuts at: 5200 442
Size: 1753
Fragments arranged by size:
2342 1753 958 483 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2892 5171 1239
Size: 1653 2279 2579
Fragments arranged by size:
2579 2279 1653
Bpu10I CC'TnA_GC
Cuts at: 2475 3249 2475
Size: 774 5737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2526 3054 3406 4636 5501 5799 2526
Size: 528 352 1230 865 298 3238
Fragments arranged by size:
3238 1230 865 528 352 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2199 5174 882
Size: 617 700 2975 2219
Fragments arranged by size:
2975 2219 700 617
BsaAI yAC'GTr
Cuts at: 493 1204 4302 493
Size: 711 3098 2702
Fragments arranged by size:
3098 2702 711
BsaBI GATnn'nnATC
Cuts at: 6505 6505
Size: 6511
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2134 4318 4700 5335
Size: 53 83 186 936 601 2184 382 635
Cuts at: 5335 275
Size: 1451
Fragments arranged by size:
2184 1451 936 635 601 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4944 5342 5616 5763 6174 4944
Size: 398 274 147 411 5281
Fragments arranged by size:
5281 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6286
Fragments arranged by size:
6286 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 3708 1186
Size: 1177 1345 3989
Fragments arranged by size:
3989 1345 1177
BseYI C'CCAG_C
Cuts at: 2777 5714 6210 2777
Size: 2937 496 3078
Fragments arranged by size:
3078 2937 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3054 1163
Size: 573 1318 4620
Fragments arranged by size:
4620 1318 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3961 4722 5750 1540
Size: 275 186 1960 761 1028 2301
Fragments arranged by size:
2301 1960 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2878 3070 4572 4657 5728 2878
Size: 192 1502 85 1071 3661
Fragments arranged by size:
3661 1502 1071 192 85
BsmI GAATG_Cn'
Cuts at: 2396 2667 4022 4115 2396
Size: 271 1355 93 4792
Fragments arranged by size:
4792 1355 271 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2878 3070 3830 4572 4657 5728
Size: 399 294 794 192 760 742 85 1071
Cuts at: 5728 1391
Size: 2174
Fragments arranged by size:
2174 1071 794 760 742 399 294 192
85
BspEI T'CCGG_A
Cuts at: 6174 6174
Size: 6511
BspHI T'CATG_A
Cuts at: 3583 4400 6130 3583
Size: 817 1730 3964
Fragments arranged by size:
3964 1730 817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3866 844
Size: 3022 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2069 3621 4398 1394
Size: 429 246 1552 777 3507
Fragments arranged by size:
3507 1552 777 429 246
BsrDI GCAATG_nn'
Cuts at: 65 1248 3615 5006 5180 65
Size: 1183 2367 1391 174 1396
Fragments arranged by size:
2367 1396 1391 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5155 5342 1700
Size: 110 3345 187 2869
Fragments arranged by size:
3345 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3232 96
Size: 3136 3375
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6511
BssSI C'ACGA_G
Cuts at: 4571 5583 4571
Size: 1012 5499
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6511
BstBI TT'CG_AA
Cuts at: 2425 5315 2425
Size: 2890 3621
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6511
BstYI r'GATC_y
Cuts at: 1972 2434 2701 3698 3841 4208 4593 4610 6062
Size: 462 267 997 143 367 385 17 1452
Cuts at: 6062 6148 6160 6506 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 997 462 385 367 346 267
143 86 17 12
BstZ17I GTA'TAC
Cuts at: 2312 2312
Size: 6511
BtgI C'CryG_G
Cuts at: 513 1065 2004 2192 513
Size: 552 939 188 4832
Fragments arranged by size:
4832 939 552 188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5772
Fragments arranged by size:
5772 683 56
BtsI GCAGTG_nn'
Cuts at: 3042 4027 4821 4841 3042
Size: 985 794 20 4712
Fragments arranged by size:
4712 985 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6476
DraI TTT'AAA
Cuts at: 2974 3187 3815 2974
Size: 213 628 5670
Fragments arranged by size:
5670 628 213
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2251 5518 809
Size: 507 935 3267 1802
Fragments arranged by size:
3267 1802 935 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 3562 3958
Size: 54 1425 50 275 18 198 1534 396
Cuts at: 3958 4847 6476 8
Size: 889 1629 43
Fragments arranged by size:
1629 1534 1425 889 396 275 198 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3958 1537
Size: 275 2146 4090
Fragments arranged by size:
4090 2146 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2011 2098 2197 2232 2619 2730 3337 4441
Size: 658 87 99 35 387 111 607 1104
Cuts at: 4441 1353
Size: 3423
Fragments arranged by size:
3423 1104 658 607 387 111 99 87
35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2111 5098 5472 5618 387
Size: 1064 138 522 2987 374 146 1280
Fragments arranged by size:
2987 1280 1064 522 374 146 138
EcoICRI GAG'CTC
Cuts at: 3068 3068
Size: 6511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2603 2754 2892 3318 3534 3731 4574
Size: 393 971 151 138 426 216 197 843
Cuts at: 4574 5171 5958 6226 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 971 843 787 597 426 393 268
216 197 151 138
EcoO109I rG'GnC_Cy
Cuts at: 2636 3451 2636
Size: 815 5696
EcoRI G'AATT_C
Cuts at: 3820 4198 3820
Size: 378 6133
FspI TGC'GCA
Cuts at: 5017 5017
Size: 6511
HaeII r_GCGC'y
Cuts at: 1678 2137 5658 1678
Size: 459 3521 2531
Fragments arranged by size:
3521 2531 459
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2283 2315 2339 2342 2371
Size: 32 135 32 711 32 24 3 29
Cuts at: 2371 2374 2485 2517 2852 2884 3339 3371 3485
Size: 3 111 32 335 32 455 32 114
Cuts at: 3485 3517 5152 5184 5226 5258 1373
Size: 32 1635 32 42 32 2626
Fragments arranged by size:
2626 1635 711 455 335 135 114 111
42 32 32 32 32 32 32 32
32 32 29 24 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2337 3430 3855 4101 669
Size: 988 281 399 1093 425 246 3079
Fragments arranged by size:
3079 1093 988 425 399 281 246
HindIII A'AGCT_T
Cuts at: 748 2850 748
Size: 2102 4409
HpaI GTT'AAC
Cuts at: 4101 4101
Size: 6511
KasI G'GCGC_C
Cuts at: 2133 2133
Size: 6511
KpnI G_GTAC'C
Cuts at: 3665 3836 3665
Size: 171 6340
MfeI C'AATT_G
Cuts at: 2562 4110 2562
Size: 1548 4963
MluI A'CGCG_T
Cuts at: 6387 6387
Size: 6511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2397 3282 5625 5809 1305
Size: 1092 885 2343 184 2007
Fragments arranged by size:
2343 2007 1092 885 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4689
Fragments arranged by size:
4689 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2746 3423 4469 4828 4987 518
Size: 648 1580 677 1046 359 159 2042
Fragments arranged by size:
2042 1580 1046 677 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2033 2045 3786 3927 4604 5752 5997 1606
Size: 427 12 1741 141 677 1148 245 2120
Fragments arranged by size:
2120 1741 1148 677 427 245 141 12
NarI GG'CG_CC
Cuts at: 2134 2134
Size: 6511
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5020
Fragments arranged by size:
5020 939 552
NdeI CA'TA_TG
Cuts at: 387 2747 387
Size: 2360 4151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6511
NotI GC'GGCC_GC
Cuts at: 3958 3958
Size: 6511
NspI r_CATG'y
Cuts at: 1132 1229 3869 5414 1132
Size: 97 2640 1545 2229
Fragments arranged by size:
2640 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6511
PciI A'CATG_T
Cuts at: 5410 5410
Size: 6511
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3749 1108
Size: 2641 3870
PmeI GTTT'AAAC
Cuts at: 3815 3815
Size: 6511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4580 4612 6093 6125 4580
Size: 32 1481 32 4966
Fragments arranged by size:
4966 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3451 3451
Size: 6511
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6511
PsiI TTA'TAA
Cuts at: 4081 4081
Size: 6511
PstI C_TGCA'G
Cuts at: 830 3863 830
Size: 3033 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6511
PvuII CAG'CTG
Cuts at: 3786 3786
Size: 6511
SacI G_AGCT'C
Cuts at: 3070 3070
Size: 6511
SalI G'TCGA_C
Cuts at: 1936 3853 1936
Size: 1917 4594
SapI GCTCTTCn'nnn_
Cuts at: 2232 2619 2232
Size: 387 6124
SbfI CC_TGCA'GG
Cuts at: 3863 3863
Size: 6511
ScaI AGT'ACT
Cuts at: 4759 6471 4759
Size: 1712 4799
SfcI C'TryA_G
Cuts at: 826 1045 2285 3164 3859 4994 5675 5866 826
Size: 219 1240 879 695 1135 681 191 1471
Fragments arranged by size:
1471 1240 1135 879 695 681 219 191
SfoI GGC'GCC
Cuts at: 2135 2135
Size: 6511
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6511
SmaI CCC'GGG
Cuts at: 1872 3838 1872
Size: 1966 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2541 2985 3069 3421 4615 5516
Size: 197 926 598 444 84 352 1194 901
Cuts at: 5516 5778 820
Size: 262 1553
Fragments arranged by size:
1553 1194 926 901 598 444 352 262
197 84
SnaBI TAC'GTA
Cuts at: 493 4302 493
Size: 3809 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6511
SphI G_CATG'C
Cuts at: 1132 3869 1132
Size: 2737 3774
SspI AAT'ATT
Cuts at: 5 52 4435 5
Size: 47 4383 2081
Fragments arranged by size:
4383 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6511
StyI C'CwwG_G
Cuts at: 513 1065 2004 3950 513
Size: 552 939 1946 3074
Fragments arranged by size:
3074 1946 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4538 4555 4708 4893 5338 325
Size: 1255 2958 17 153 185 445 1498
Fragments arranged by size:
2958 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2517 3232 4757
Size: 274 80 33 51 1443 540 715 1525
Cuts at: 4757 6469 96
Size: 1712 138
Fragments arranged by size:
1712 1525 1443 715 540 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3544 3576 6471 6503 3544
Size: 32 2895 32 3552
Fragments arranged by size:
3552 2895 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6511
XbaI T'CTAG_A
Cuts at: 3847 3847
Size: 6511
XhoI C'TCGA_G
Cuts at: 1943 2985 1943
Size: 1042 5469
XmaI C'CCGG_G
Cuts at: 1870 3836 1870
Size: 1966 4545
XmnI GAAnn'nnTTC
Cuts at: 2557 4640 2557
Size: 2083 4428
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4319 276
Size: 53 83 186 936 2785 2468
Fragments arranged by size:
2785 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SrfI SwaI XcmI