Restriction map for
pFN21AE3427
(Circular) (Six-base) MAPSORT of: pFN21AE3427.seq Check: 7947 from: 1 to: 6511
pFN21AE3427 6511 bp
With 182 enzymes: SgfI *
March 28, 2012 12:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4321 278
Size: 53 83 186 936 2785 2468
Fragments arranged by size:
2785 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3854 1937
Size: 1917 4594
Acc65I G'GTAC_C
Cuts at: 3832 3832
Size: 6511
AclI AA'CG_TT
Cuts at: 1563 2810 4638 5011 1563
Size: 1247 1828 373 3063
Fragments arranged by size:
3063 1828 1247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2254 2740 3260 3276 4574 5958 6226 1632
Size: 622 486 520 16 1298 1384 268 1917
Fragments arranged by size:
1917 1384 1298 622 520 486 268 16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6314
AflIII A'CryG_T
Cuts at: 1203 3486 5410 6387 1203
Size: 2283 1924 977 1327
Fragments arranged by size:
2283 1924 1327 977
AgeI A'CCGG_T
Cuts at: 5342 5342
Size: 6511
AhdI GACnn_n'nnGTC
Cuts at: 2642 5240 2642
Size: 2598 3913
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6511
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3521 1184
Size: 2337 4174
AlwNI CAG_nnn'CTG
Cuts at: 2237 2309 3676 4254 5826 2237
Size: 72 1367 578 1572 2922
Fragments arranged by size:
2922 1572 1367 578 72
ApaI G_GGCC'C
Cuts at: 2159 2159
Size: 6511
ApaLI G'TGCA_C
Cuts at: 3800 4568 5724 3800
Size: 768 1156 4587
Fragments arranged by size:
4587 1156 768
ApoI r'AATT_y
Cuts at: 1493 3820 4046 4198 6307 1493
Size: 2327 226 152 2109 1697
Fragments arranged by size:
2327 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5065 160
Size: 872 4033 1606
Fragments arranged by size:
4033 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
AvaI C'yCGr_G
Cuts at: 1870 1943 2730 3836 1870
Size: 73 787 1106 4545
Fragments arranged by size:
4545 1106 787 73
AvrII C'CTAG_G
Cuts at: 3581 3581
Size: 6511
BamHI G'GATC_C
Cuts at: 3841 4208 3841
Size: 367 6144
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3284 3832 5287 618
Size: 325 217 627 1497 548 1455 1842
Fragments arranged by size:
1842 1497 1455 627 548 325 217
BanII G_rGCy'C
Cuts at: 1391 2042 2150 2159 3518 3830 1391
Size: 651 108 9 1359 312 4072
Fragments arranged by size:
4072 1359 651 312 108 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2319 3006 928
Size: 971 420 687 4433
Fragments arranged by size:
4433 971 687 420
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4702 4736 1903
Size: 34 2765 34 3678
Fragments arranged by size:
3678 2765 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3554 4403 5613 3554
Size: 849 1210 4452
Fragments arranged by size:
4452 1210 849
BclI T'GATC_A
Cuts at: 1548 2403 2686 1548
Size: 855 283 5373
Fragments arranged by size:
5373 855 283
BfrBI ATG'CAT
Cuts at: 2963 2963
Size: 6511
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2154 5122 136
Size: 107 122 71 631 605 482 2968 1525
Fragments arranged by size:
2968 1525 631 605 482 122 107 71
BglII A'GATC_T
Cuts at: 2021 6506 2021
Size: 4485 2026
BlpI GC'TnA_GC
Cuts at: 2144 3928 2144
Size: 1784 4727
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2583 3214 3804 4572 5728 1790
Size: 369 424 631 590 768 1156 2573
Fragments arranged by size:
2573 1156 768 631 590 424 369
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6511
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2116 3075 3424
Size: 416 53 83 399 24 699 959 349
Cuts at: 3424 4242 5200 442
Size: 818 958 1753
Fragments arranged by size:
1753 959 958 818 699 416 399 349
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3590 1056
Size: 2534 3977
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3633 3633
Size: 6511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 2640 2989 3043 3451 5171 1239
Size: 977 424 349 54 408 1720 2579
Fragments arranged by size:
2579 1720 977 424 408 349 54
Bpu10I CC'TnA_GC
Cuts at: 2036 2036
Size: 6511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2645 4636 5501 5799 2645
Size: 1991 865 298 3357
Fragments arranged by size:
3357 1991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2965 5174 882
Size: 617 1466 2209 2219
Fragments arranged by size:
2219 2209 1466 617
BsaAI yAC'GTr
Cuts at: 493 1204 4302 493
Size: 711 3098 2702
Fragments arranged by size:
3098 2702 711
BsaBI GATnn'nnATC
Cuts at: 2469 6505 2469
Size: 4036 2475
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4318 4700 5335 275
Size: 53 83 186 936 2785 382 635 1451
Fragments arranged by size:
2785 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4944 5342 5616 5763 6174 4944
Size: 398 274 147 411 5281
Fragments arranged by size:
5281 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6286
Fragments arranged by size:
6286 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2351 3108 3351 3463 3772 3775 1186
Size: 940 225 757 243 112 309 3 3922
Fragments arranged by size:
3922 940 757 309 243 225 112 3
BseYI C'CCAG_C
Cuts at: 3409 5714 6210 3409
Size: 2305 496 3710
Fragments arranged by size:
3710 2305 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2690 1163
Size: 573 954 4984
Fragments arranged by size:
4984 954 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3961 4722 5750 1540
Size: 275 186 1960 761 1028 2301
Fragments arranged by size:
2301 1960 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2042 2050 2225 3518 3696 3804 4572 4657 5728
Size: 8 175 1293 178 108 768 85 1071
Cuts at: 5728 2042
Size: 2825
Fragments arranged by size:
2825 1293 1071 768 178 175 108 85
8
BsmI GAATG_Cn'
Cuts at: 2963 4022 4115 2963
Size: 1059 93 5359
Fragments arranged by size:
5359 1059 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2050 2150 2159 2225 2583 3214
Size: 399 252 8 100 9 66 358 631
Cuts at: 3214 3518 3696 3804 3830 4572 4657 5728 1391
Size: 304 178 108 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 631 399 358 304 252
178 108 100 85 66 26 9 8
BspEI T'CCGG_A
Cuts at: 6174 6174
Size: 6511
BspHI T'CATG_A
Cuts at: 4400 6130 4400
Size: 1730 4781
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3684 3866 844
Size: 2840 182 3489
Fragments arranged by size:
3489 2840 182
BsrBI CCG'CTC
Cuts at: 1394 1823 2254 4398 1394
Size: 429 431 2144 3507
Fragments arranged by size:
3507 2144 431 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2363 2496 2569 3463 5006 5180 65
Size: 1183 1115 133 73 894 1543 174 1396
Fragments arranged by size:
1543 1396 1183 1115 894 174 133 73
BsrFI r'CCGG_y
Cuts at: 1700 1810 3756 5155 5342 1700
Size: 110 1946 1399 187 2869
Fragments arranged by size:
2869 1946 1399 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6511
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6511
BssSI C'ACGA_G
Cuts at: 4571 5583 4571
Size: 1012 5499
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2713 3458 3676 3700 794
Size: 1919 745 218 24 3605
Fragments arranged by size:
3605 1919 745 218 24
BstBI TT'CG_AA
Cuts at: 2997 5315 2997
Size: 2318 4193
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6511
BstXI CCAn_nnnn'nTGG
Cuts at: 2390 2551 2390
Size: 161 6350
BstYI r'GATC_y
Cuts at: 1972 2021 2464 3841 4208 4593 4610 6062 6148
Size: 49 443 1377 367 385 17 1452 86
Cuts at: 6148 6160 6506 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1377 443 385 367 346 86
49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2151 2331 513
Size: 552 1086 180 4693
Fragments arranged by size:
4693 1086 552 180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5772
Fragments arranged by size:
5772 683 56
BtsI GCAGTG_nn'
Cuts at: 2767 4027 4821 4841 2767
Size: 1260 794 20 4437
Fragments arranged by size:
4437 1260 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6476
DraI TTT'AAA
Cuts at: 3815 3815
Size: 6511
DraIII CAC_nnn'GTG
Cuts at: 3209 3209
Size: 6511
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5518 809
Size: 507 4202 1802
Fragments arranged by size:
4202 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3403 3754 3958
Size: 54 1425 50 275 18 1573 351 204
Cuts at: 3958 4847 6476 8
Size: 889 1629 43
Fragments arranged by size:
1629 1573 1425 889 351 275 204 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3958 1537
Size: 275 2146 4090
Fragments arranged by size:
4090 2146 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4441 1353
Size: 3088 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5098 5472 5618 387
Size: 1064 138 3509 374 146 1280
Fragments arranged by size:
3509 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2040 3516 2040
Size: 1476 5035
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2254 2640 2740 2989 3043 3260
Size: 393 584 38 386 100 249 54 217
Cuts at: 3260 3276 3451 4574 5171 5958 6226 1239
Size: 16 175 1123 597 787 268 1524
Fragments arranged by size:
1524 1123 787 597 584 393 386 268
249 217 175 100 54 38 16
EcoO109I rG'GnC_Cy
Cuts at: 2242 2306 3414 3654 2242
Size: 64 1108 240 5099
Fragments arranged by size:
5099 1108 240 64
EcoRI G'AATT_C
Cuts at: 3820 4198 3820
Size: 378 6133
FspI TGC'GCA
Cuts at: 2984 5017 2984
Size: 2033 4478
FspAI rTGC'GCAy
Cuts at: 2984 2984
Size: 6511
HaeII r_GCGC'y
Cuts at: 1678 2144 3615 5658 1678
Size: 466 1471 2043 2531
Fragments arranged by size:
2531 2043 1471 466
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2013 2045 2276 2308 2436
Size: 32 135 32 441 32 231 32 128
Cuts at: 2436 2468 2628 2660 5152 5184 5226 5258 1373
Size: 32 160 32 2492 32 42 32 2626
Fragments arranged by size:
2626 2492 441 231 160 135 128 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3855 4101 669
Size: 988 281 1917 246 3079
Fragments arranged by size:
3079 1917 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2663 748
Size: 1915 4596
HpaI GTT'AAC
Cuts at: 4101 4101
Size: 6511
KpnI G_GTAC'C
Cuts at: 3836 3836
Size: 6511
MfeI C'AATT_G
Cuts at: 4110 4110
Size: 6511
MluI A'CGCG_T
Cuts at: 6387 6387
Size: 6511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2661 3146 3672 5625 5809 1305
Size: 1356 485 526 1953 184 2007
Fragments arranged by size:
2007 1953 1356 526 485 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3405 10
Size: 54 1425 343 1573 3116
Fragments arranged by size:
3116 1573 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2444 2489 2630 3011 3704 4469 4828
Size: 648 1278 45 141 381 693 765 359
Cuts at: 4828 4987 518
Size: 159 2042
Fragments arranged by size:
2042 1278 765 693 648 381 359 159
141 45
MspA1I CmG'CkG
Cuts at: 1606 2153 3175 3409 3927 4604 5752 5997 1606
Size: 547 1022 234 518 677 1148 245 2120
Fragments arranged by size:
2120 1148 1022 677 547 518 245 234
NaeI GCC'GGC
Cuts at: 3758 3758
Size: 6511
NcoI C'CATG_G
Cuts at: 513 1065 2331 513
Size: 552 1266 4693
Fragments arranged by size:
4693 1266 552
NdeI CA'TA_TG
Cuts at: 387 2959 387
Size: 2572 3939
NgoMIV G'CCGG_C
Cuts at: 3756 3756
Size: 6511
NheI G'CTAG_C
Cuts at: 1052 3586 1052
Size: 2534 3977
NotI GC'GGCC_GC
Cuts at: 3958 3958
Size: 6511
NruI TCG'CGA
Cuts at: 2044 2044
Size: 6511
NsiI A_TGCA'T
Cuts at: 2965 2965
Size: 6511
NspI r_CATG'y
Cuts at: 1132 1229 2054 3490 3508 3703 3869 5414 1132
Size: 97 825 1436 18 195 166 1545 2229
Fragments arranged by size:
2229 1545 1436 825 195 166 97 18
PasI CC'CwG_GG
Cuts at: 1348 3245 1348
Size: 1897 4614
PciI A'CATG_T
Cuts at: 3486 5410 3486
Size: 1924 4587
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6511
PmeI GTTT'AAAC
Cuts at: 3815 3815
Size: 6511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2244 2816 2848 4580 4612 6093 6125 2212
Size: 32 572 32 1732 32 1481 32 2598
Fragments arranged by size:
2598 1732 1481 572 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2242 3414 2242
Size: 1172 5339
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6511
PsiI TTA'TAA
Cuts at: 4081 4081
Size: 6511
PspOMI G'GGCC_C
Cuts at: 2155 2155
Size: 6511
PspXI vC'TCGA_Gb
Cuts at: 2730 2730
Size: 6511
PstI C_TGCA'G
Cuts at: 830 2029 2305 3863 830
Size: 1199 276 1558 3478
Fragments arranged by size:
3478 1558 1199 276
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6511
PvuII CAG'CTG
Cuts at: 3175 3409 3175
Size: 234 6277
SacI G_AGCT'C
Cuts at: 2042 3518 2042
Size: 1476 5035
SacII CC_GC'GG
Cuts at: 2154 2154
Size: 6511
SalI G'TCGA_C
Cuts at: 1936 3853 1936
Size: 1917 4594
SbfI CC_TGCA'GG
Cuts at: 3863 3863
Size: 6511
ScaI AGT'ACT
Cuts at: 2275 2480 4759 6471 2275
Size: 205 2279 1712 2315
Fragments arranged by size:
2315 2279 1712 205
SfcI C'TryA_G
Cuts at: 826 1045 2025 2301 3859 4994 5675 5866 826
Size: 219 980 276 1558 1135 681 191 1471
Fragments arranged by size:
1558 1471 1135 980 681 276 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6511
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6511
SmaI CCC'GGG
Cuts at: 1872 3838 1872
Size: 1966 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2660 2730 4615 5516 5778 820
Size: 197 926 717 70 1885 901 262 1553
Fragments arranged by size:
1885 1553 926 901 717 262 197 70
SnaBI TAC'GTA
Cuts at: 493 4302 493
Size: 3809 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6511
SphI G_CATG'C
Cuts at: 1132 3703 3869 1132
Size: 2571 166 3774
Fragments arranged by size:
3774 2571 166
SspI AAT'ATT
Cuts at: 5 52 4435 5
Size: 47 4383 2081
Fragments arranged by size:
4383 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6511
StyI C'CwwG_G
Cuts at: 513 1065 2331 3581 3950 513
Size: 552 1266 1250 369 3074
Fragments arranged by size:
3074 1266 1250 552 369
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2788 2901 3736 4538 4555 4708 4893
Size: 1255 1208 113 835 802 17 153 185
Cuts at: 4893 5338 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1208 835 802 445 185 153
113 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2273 2478 4757
Size: 274 80 33 51 1443 296 205 2279
Cuts at: 4757 6469 96
Size: 1712 138
Fragments arranged by size:
2279 1712 1443 296 274 205 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6471 6503 6471
Size: 32 6479
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6511
XbaI T'CTAG_A
Cuts at: 3847 3847
Size: 6511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2329 2551 2677 2329
Size: 222 126 6163
Fragments arranged by size:
6163 222 126
XhoI C'TCGA_G
Cuts at: 1943 2730 1943
Size: 787 5724
XmaI C'CCGG_G
Cuts at: 1870 3836 1870
Size: 1966 4545
XmnI GAAnn'nnTTC
Cuts at: 2378 2810 4640 2378
Size: 432 1830 4249
Fragments arranged by size:
4249 1830 432
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4319 276
Size: 53 83 186 936 2785 2468
Fragments arranged by size:
2785 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BsiWI
BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI KasI
NarI PacI PfoI PmlI PsrI RsrII SanDI SapI
SexAI SfiI SfoI SrfI SwaI