Restriction map for
pFN21AE3425
(Circular) (Six-base) MAPSORT of: pFN21AE3425.seq Check: 1091 from: 1 to: 6331
pFN21AE3425 6331 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2329 2771 3040 2329
Size: 442 269 5620
Fragments arranged by size:
5620 442 269
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2690 4141 278
Size: 53 83 186 936 1154 1451 2468
Fragments arranged by size:
2468 1451 1154 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3165 3674 1937
Size: 1228 509 4594
Fragments arranged by size:
4594 1228 509
Acc65I G'GTAC_C
Cuts at: 3652 3652
Size: 6331
AclI AA'CG_TT
Cuts at: 1563 4458 4831 1563
Size: 2895 373 3063
Fragments arranged by size:
3063 2895 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4394 5778 6046 1632
Size: 2762 1384 268 1917
Fragments arranged by size:
2762 1917 1384 268
AfeI AGC'GCT
Cuts at: 2803 2803
Size: 6331
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6134
AflIII A'CryG_T
Cuts at: 1203 2968 3147 5230 6207 1203
Size: 1765 179 2083 977 1327
Fragments arranged by size:
2083 1765 1327 977 179
AgeI A'CCGG_T
Cuts at: 2131 5162 2131
Size: 3031 3300
AhdI GACnn_n'nnGTC
Cuts at: 5060 5060
Size: 6331
AleI CACnn'nnGTG
Cuts at: 1166 3066 3348 1166
Size: 1900 282 4149
Fragments arranged by size:
4149 1900 282
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2648 1184
Size: 1464 4867
AlwNI CAG_nnn'CTG
Cuts at: 2879 3055 3137 4074 5646 2879
Size: 176 82 937 1572 3564
Fragments arranged by size:
3564 1572 937 176 82
ApaI G_GGCC'C
Cuts at: 2682 3095 3234 2682
Size: 413 139 5779
Fragments arranged by size:
5779 413 139
ApaLI G'TGCA_C
Cuts at: 2299 3563 4388 5544 2299
Size: 1264 825 1156 3086
Fragments arranged by size:
3086 1264 1156 825
ApoI r'AATT_y
Cuts at: 1493 3640 3866 4018 6127 1493
Size: 2147 226 152 2109 1697
Fragments arranged by size:
2147 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3378 3378
Size: 6331
AseI AT'TA_AT
Cuts at: 160 1032 4885 160
Size: 872 3853 1606
Fragments arranged by size:
3853 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6331
AvaI C'yCGr_G
Cuts at: 1870 1943 2452 2620 2674 2681 2763 2836 2929
Size: 73 509 168 54 7 82 73 93
Cuts at: 2929 3656 1870
Size: 727 4545
Fragments arranged by size:
4545 727 509 168 93 82 73 73
54 7
BamHI G'GATC_C
Cuts at: 3661 4028 3661
Size: 367 5964
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2908 3100 3269 3277 3350
Size: 325 217 627 1121 192 169 8 73
Cuts at: 3350 3362 3454 3478 3652 5107 618
Size: 12 92 24 174 1455 1842
Fragments arranged by size:
1842 1455 1121 627 325 217 192 174
169 92 73 24 12 8
BanII G_rGCy'C
Cuts at: 1391 2561 2682 2843 2861 3095 3234 3296 3650
Size: 1170 121 161 18 234 139 62 354
Cuts at: 3650 1391
Size: 4072
Fragments arranged by size:
4072 1170 354 234 161 139 121 62
18
BbeI G_GCGC'C
Cuts at: 2912 3104 3273 3281 3366 3458 2912
Size: 192 169 8 85 92 5785
Fragments arranged by size:
5785 192 169 92 85 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2464 928
Size: 971 565 4795
Fragments arranged by size:
4795 971 565
BbvCI CC'TCA_GC
Cuts at: 3392 3392
Size: 6331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2290 2324 4522 4556 1903
Size: 34 353 34 2198 34 3678
Fragments arranged by size:
3678 2198 353 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4223 5433 4223
Size: 1210 5121
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6331
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2114 2622 2814
Size: 107 122 71 631 605 442 508 192
Cuts at: 2814 2856 2906 3452 4942 136
Size: 42 50 546 1490 1525
Fragments arranged by size:
1525 1490 631 605 546 508 442 192
122 107 71 50 42
BglII A'GATC_T
Cuts at: 6326 6326
Size: 6331
BlpI GC'TnA_GC
Cuts at: 3299 3748 3299
Size: 449 5882
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2303 2682 3095 3234 3483 3567 4392
Size: 247 266 379 413 139 249 84 825
Cuts at: 4392 5548 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 825 413 379 266 249 247
139 84
BmgBI CAC'GTC
Cuts at: 1318 3031 1318
Size: 1713 4618
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4062 5020 442
Size: 416 53 83 399 24 2645 958 1753
Fragments arranged by size:
2645 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2850 1056
Size: 1794 4537
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 6331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2529 3020 4991 1239
Size: 1290 491 1971 2579
Fragments arranged by size:
2579 1971 1290 491
Bpu10I CC'TnA_GC
Cuts at: 2713 3392 2713
Size: 679 5652
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 4456 5321 5619 2234
Size: 2222 865 298 2946
Fragments arranged by size:
2946 2222 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4994 882
Size: 617 3495 2219
Fragments arranged by size:
3495 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4122 493
Size: 711 2918 2702
Fragments arranged by size:
2918 2702 711
BsaBI GATnn'nnATC
Cuts at: 6325 6325
Size: 6331
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2084 2093 2687 2909
Size: 53 83 186 936 551 9 594 222
Cuts at: 2909 2948 2994 3101 3168 3270 3278 3363 3455
Size: 39 46 107 67 102 8 85 92
Cuts at: 3455 4138 4520 5155 275
Size: 683 382 635 1451
Fragments arranged by size:
1451 936 683 635 594 551 382 222
186 107 102 92 85 83 67 53
46 39 9 8
BsaWI w'CCGG_w
Cuts at: 2131 2531 2867 3619 4764 5162 5436 5583 5994
Size: 400 336 752 1145 398 274 147 411
Cuts at: 5994 2131
Size: 2468
Fragments arranged by size:
2468 1145 752 411 400 398 336 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2993 3023 1178
Size: 30 165 30 1590 30 4486
Fragments arranged by size:
4486 1590 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2384 2840 1186
Size: 1198 456 4677
Fragments arranged by size:
4677 1198 456
BseYI C'CCAG_C
Cuts at: 2027 2917 3155 3289 3373 5534 6030 2027
Size: 890 238 134 84 2161 496 2328
Fragments arranged by size:
2328 2161 890 496 238 134 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3056 1163
Size: 573 1320 4438
Fragments arranged by size:
4438 1320 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2118 3084 3546 3781 4542 5570
Size: 275 186 117 966 462 235 761 1028
Cuts at: 5570 1540
Size: 2301
Fragments arranged by size:
2301 1028 966 761 462 275 235 186
117
BsiHKAI G_wGCw'C
Cuts at: 2303 3567 4392 4477 5548 2303
Size: 1264 825 85 1071 3086
Fragments arranged by size:
3086 1264 1071 825 85
BsmI GAATG_Cn'
Cuts at: 3842 3935 3842
Size: 93 6238
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2303 2561 2682 2843 2861 3095
Size: 399 247 266 258 121 161 18 234
Cuts at: 3095 3234 3296 3483 3567 3650 4392 4477 5548
Size: 139 62 187 84 83 742 85 1071
Cuts at: 5548 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 266 258 247 234
187 161 139 121 85 84 83 62
18
BspEI T'CCGG_A
Cuts at: 3619 5994 3619
Size: 2375 3956
BspHI T'CATG_A
Cuts at: 4220 5950 4220
Size: 1730 4601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2329 2771 3040 3619 3686 844
Size: 1485 442 269 579 67 3489
Fragments arranged by size:
3489 1485 579 442 269 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2513 3309 3385 3540 4218 1394
Size: 429 690 796 76 155 678 3507
Fragments arranged by size:
3507 796 690 678 429 155 76
BsrDI GCAATG_nn'
Cuts at: 65 1248 4826 5000 65
Size: 1183 3578 174 1396
Fragments arranged by size:
3578 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2131 2641 2905 2985 3083 3266 3470
Size: 110 321 510 264 80 98 183 204
Cuts at: 3470 4975 5162 1700
Size: 1505 187 2869
Fragments arranged by size:
2869 1505 510 321 264 204 187 183
110 98 80
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6331
BssHII G'CGCG_C
Cuts at: 1925 2214 2216 2381 3378 1925
Size: 289 2 165 997 4878
Fragments arranged by size:
4878 997 289 165 2
BssSI C'ACGA_G
Cuts at: 4391 5403 4391
Size: 1012 5319
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3137 794
Size: 2343 3988
BstBI TT'CG_AA
Cuts at: 5135 5135
Size: 6331
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6331
BstXI CCAn_nnnn'nTGG
Cuts at: 3068 3068
Size: 6331
BstYI r'GATC_y
Cuts at: 1972 3661 4028 4413 4430 5882 5968 5980 6326
Size: 1689 367 385 17 1452 86 12 346
Cuts at: 6326 1972
Size: 1977
Fragments arranged by size:
1977 1689 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2109 2198 2230 2523 2936 3067
Size: 552 939 105 89 32 293 413 131
Cuts at: 3067 3120 3273 3417 3436 3504 513
Size: 53 153 144 19 68 3340
Fragments arranged by size:
3340 939 552 413 293 153 144 131
105 89 68 53 32 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2109 2293 2745 487
Size: 683 56 883 184 452 4073
Fragments arranged by size:
4073 883 683 452 184 56
BtsI GCAGTG_nn'
Cuts at: 2406 3465 3847 4641 4661 2406
Size: 1059 382 794 20 4076
Fragments arranged by size:
4076 1059 794 382 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6331
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6296
DraI TTT'AAA
Cuts at: 3635 3635
Size: 6331
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5338 809
Size: 507 4022 1802
Fragments arranged by size:
4022 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2427 3081
Size: 54 1425 50 275 18 285 312 654
Cuts at: 3081 3264 3543 3778 4667 6296 8
Size: 183 279 235 889 1629 43
Fragments arranged by size:
1629 1425 889 654 312 285 279 275
235 183 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2115 3081 3543 3778 1537
Size: 275 303 966 462 235 4090
Fragments arranged by size:
4090 966 462 303 275 235
EarI CTCTTCn'nnn_
Cuts at: 1353 2883 4261 1353
Size: 1530 1378 3423
Fragments arranged by size:
3423 1530 1378
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2553 2937 3485 4918 5292 5438
Size: 1064 138 964 384 548 1433 374 146
Cuts at: 5438 387
Size: 1280
Fragments arranged by size:
1433 1280 1064 964 548 384 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2529 3020 4394 4991 5778 6046 1239
Size: 393 897 491 1374 597 787 268 1524
Fragments arranged by size:
1524 1374 897 787 597 491 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2504 2678 3073 3092 3200 3230 2504
Size: 174 395 19 108 30 5605
Fragments arranged by size:
5605 395 174 108 30 19
EcoRI G'AATT_C
Cuts at: 3640 4018 3640
Size: 378 5953
EcoRV GAT'ATC
Cuts at: 2959 2959
Size: 6331
FseI GG_CCGG'CC
Cuts at: 3087 3087
Size: 6331
FspI TGC'GCA
Cuts at: 2351 4837 2351
Size: 2486 3845
HaeII r_GCGC'y
Cuts at: 1678 2320 2348 2426 2522 2805 2912 3104 3273
Size: 642 28 78 96 283 107 192 169
Cuts at: 3273 3281 3290 3366 3458 3618 5478 1678
Size: 8 9 76 92 160 1860 2531
Fragments arranged by size:
2531 1860 642 283 192 169 160 107
96 92 78 76 28 9 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2723 2755 4972 5004 5046
Size: 32 135 32 1151 32 2217 32 42
Cuts at: 5046 5078 1373
Size: 32 2626
Fragments arranged by size:
2626 2217 1151 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3166 3610 3675 3921 669
Size: 988 281 1228 444 65 246 3079
Fragments arranged by size:
3079 1228 988 444 281 246 65
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6331
HpaI GTT'AAC
Cuts at: 3921 3921
Size: 6331
KasI G'GCGC_C
Cuts at: 2908 3100 3269 3277 3362 3454 2908
Size: 192 169 8 85 92 5785
Fragments arranged by size:
5785 192 169 92 85 8
KpnI G_GTAC'C
Cuts at: 3656 3656
Size: 6331
MfeI C'AATT_G
Cuts at: 3930 3930
Size: 6331
MluI A'CGCG_T
Cuts at: 2968 3147 6207 2968
Size: 179 3060 3092
Fragments arranged by size:
3092 3060 179
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5445 5629 1305
Size: 4140 184 2007
Fragments arranged by size:
4140 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4509
Fragments arranged by size:
4509 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3066 3348 4289 4648 4807 518
Size: 648 1900 282 941 359 159 2042
Fragments arranged by size:
2042 1900 941 648 359 282 159
MspA1I CmG'CkG
Cuts at: 1606 2111 2120 2200 2395 2525 2546 2632 2790
Size: 505 9 80 195 130 21 86 158
Cuts at: 2790 2944 2953 3137 3191 3220 3325 3373 3419
Size: 154 9 184 54 29 105 48 46
Cuts at: 3419 3438 3551 3747 4424 5572 5817 1606
Size: 19 113 196 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 505 245 196 195 184
158 154 130 113 105 86 80 54
48 46 29 21 19 9 9
NaeI GCC'GGC
Cuts at: 2907 3085 3268 3472 2907
Size: 178 183 204 5766
Fragments arranged by size:
5766 204 183 178
NarI GG'CG_CC
Cuts at: 2909 3101 3270 3278 3363 3455 2909
Size: 192 169 8 85 92 5785
Fragments arranged by size:
5785 192 169 92 85 8
NcoI C'CATG_G
Cuts at: 513 1065 2004 2230 3273 3504 513
Size: 552 939 226 1043 231 3340
Fragments arranged by size:
3340 1043 939 552 231 226
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6331
NgoMIV G'CCGG_C
Cuts at: 2905 3083 3266 3470 2905
Size: 178 183 204 5766
Fragments arranged by size:
5766 204 183 178
NheI G'CTAG_C
Cuts at: 1052 2846 1052
Size: 1794 4537
NotI GC'GGCC_GC
Cuts at: 2115 3778 2115
Size: 1663 4668
NspI r_CATG'y
Cuts at: 1132 1229 3689 5234 1132
Size: 97 2460 1545 2229
Fragments arranged by size:
2460 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6331
PciI A'CATG_T
Cuts at: 5230 5230
Size: 6331
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2236 1108
Size: 1128 5203
PfoI T'CCnGG_A
Cuts at: 2997 2997
Size: 6331
PmeI GTTT'AAAC
Cuts at: 3635 3635
Size: 6331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4400 4432 5913 5945 4400
Size: 32 1481 32 4786
Fragments arranged by size:
4786 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2504 2504
Size: 6331
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6331
PsiI TTA'TAA
Cuts at: 3901 3901
Size: 6331
PspOMI G'GGCC_C
Cuts at: 2678 3091 3230 2678
Size: 413 139 5779
Fragments arranged by size:
5779 413 139
PstI C_TGCA'G
Cuts at: 830 2394 2610 3133 3142 3225 3330 3683 830
Size: 1564 216 523 9 83 105 353 3478
Fragments arranged by size:
3478 1564 523 353 216 105 83 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6331
PvuII CAG'CTG
Cuts at: 2395 2546 2790 3137 2395
Size: 151 244 347 5589
Fragments arranged by size:
5589 347 244 151
SacII CC_GC'GG
Cuts at: 2112 2201 2526 3420 3439 2112
Size: 89 325 894 19 5004
Fragments arranged by size:
5004 894 325 89 19
SalI G'TCGA_C
Cuts at: 1936 3164 3673 1936
Size: 1228 509 4594
Fragments arranged by size:
4594 1228 509
SbfI CC_TGCA'GG
Cuts at: 3683 3683
Size: 6331
ScaI AGT'ACT
Cuts at: 4579 6291 4579
Size: 1712 4619
SfcI C'TryA_G
Cuts at: 826 1045 2390 2606 3129 3138 3221 3326 3679
Size: 219 1345 216 523 9 83 105 353
Cuts at: 3679 4814 5495 5686 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1345 1135 681 523 353 219 216
191 105 83 9
SfoI GGC'GCC
Cuts at: 2910 3102 3271 3279 3364 3456 2910
Size: 192 169 8 85 92 5785
Fragments arranged by size:
5785 192 169 92 85 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6331
SgrAI Cr'CCGG_yG
Cuts at: 1700 2641 2905 2985 1700
Size: 941 264 80 5046
Fragments arranged by size:
5046 941 264 80
SmaI CCC'GGG
Cuts at: 1872 2622 2683 2838 3658 1872
Size: 750 61 155 820 4545
Fragments arranged by size:
4545 820 750 155 61
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 4435 5336 5598 820
Size: 197 926 306 2186 901 262 1553
Fragments arranged by size:
2186 1553 926 901 306 262 197
SnaBI TAC'GTA
Cuts at: 493 4122 493
Size: 3629 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6331
SphI G_CATG'C
Cuts at: 1132 3689 1132
Size: 2557 3774
SrfI GCCC'GGGC
Cuts at: 2622 2838 2622
Size: 216 6115
SspI AAT'ATT
Cuts at: 5 52 4255 5
Size: 47 4203 2081
Fragments arranged by size:
4203 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6331
StyI C'CwwG_G
Cuts at: 513 1065 2004 2230 3273 3504 3770 513
Size: 552 939 226 1043 231 266 3074
Fragments arranged by size:
3074 1043 939 552 266 231 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3113 3517 4358 4375 4528 4713 5158
Size: 1255 1533 404 841 17 153 185 445
Cuts at: 5158 325
Size: 1498
Fragments arranged by size:
1533 1498 1255 841 445 404 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4577 6289 96
Size: 274 80 33 51 1443 2600 1712 138
Fragments arranged by size:
2600 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2273 2305 2958 2990 6291 6323 2273
Size: 32 653 32 3301 32 2281
Fragments arranged by size:
3301 2281 653 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2689 1316
Size: 1373 4958
XbaI T'CTAG_A
Cuts at: 3667 3667
Size: 6331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3472 3472
Size: 6331
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6331
XmaI C'CCGG_G
Cuts at: 1870 2620 2681 2836 3656 1870
Size: 750 61 155 820 4545
Fragments arranged by size:
4545 820 750 155 61
XmnI GAAnn'nnTTC
Cuts at: 4460 4460
Size: 6331
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2688 4139 276
Size: 53 83 186 936 1154 1451 2468
Fragments arranged by size:
2468 1451 1154 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI FalI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SwaI