Restriction map for
pFN21AE3424
(Circular) (Six-base) MAPSORT of: pFN21AE3424.seq Check: 4132 from: 1 to: 6217
pFN21AE3424 6217 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2866 2962 2866
Size: 96 6121
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3062 3173 4280 5664 5932 1632
Size: 1430 111 1107 1384 268 1917
Fragments arranged by size:
1917 1430 1384 1107 268 111
AfeI AGC'GCT
Cuts at: 3047 3047
Size: 6217
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 3503 5116 6093 1203
Size: 2300 1613 977 1327
Fragments arranged by size:
2300 1613 1327 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2820 1184
Size: 1636 4581
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 6185
AlwNI CAG_nnn'CTG
Cuts at: 2185 2275 2473 2563 2674 2740 3391 3960 5532
Size: 90 198 90 111 66 651 569 1572
Cuts at: 5532 2185
Size: 2870
Fragments arranged by size:
2870 1572 651 569 198 111 90 90
66
ApaI G_GGCC'C
Cuts at: 2168 2246 2168
Size: 78 6139
ApaLI G'TGCA_C
Cuts at: 4274 5430 4274
Size: 1156 5061
ApoI r'AATT_y
Cuts at: 1493 3526 3752 3904 6013 1493
Size: 2033 226 152 2109 1697
Fragments arranged by size:
2109 2033 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 2167 2366 2896 3197 3542 1870
Size: 73 224 199 530 301 345 4545
Fragments arranged by size:
4545 530 345 301 224 199 73
BamHI G'GATC_C
Cuts at: 3547 3914 3547
Size: 367 5850
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2222 2849 2929 3438 3538
Size: 325 217 627 435 627 80 509 100
Cuts at: 3538 4993 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 627 509 435 325 217
100 80
BanII G_rGCy'C
Cuts at: 1391 2168 2246 2373 2616 2969 3198 3405 3485
Size: 777 78 127 243 353 229 207 80
Cuts at: 3485 3536 1391
Size: 51 4072
Fragments arranged by size:
4072 777 353 243 229 207 127 80
78 51
BbeI G_GCGC'C
Cuts at: 2226 3442 2226
Size: 1216 5001
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4408 4442 1903
Size: 34 2471 34 3678
Fragments arranged by size:
3678 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3065 4109 5319 3065
Size: 1044 1210 3963
Fragments arranged by size:
3963 1210 1044
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2568 4828
Size: 107 122 71 631 605 337 559 2260
Cuts at: 4828 136
Size: 1525
Fragments arranged by size:
2260 1525 631 605 559 337 122 107
71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 2777 3634 2777
Size: 857 5360
Bme1580I G_kGCm'C
Cuts at: 1790 2096 2168 2246 2282 2852 4278 5434 1790
Size: 306 72 78 36 570 1426 1156 2573
Fragments arranged by size:
2573 1426 1156 570 306 78 72 36
BmgBI CAC'GTC
Cuts at: 1318 2982 1318
Size: 1664 4553
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2928 3948 4906
Size: 416 53 83 399 24 1511 1020 958
Cuts at: 4906 442
Size: 1753
Fragments arranged by size:
1753 1511 1020 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 3419 3470 3481 4877 1239
Size: 1211 969 51 11 1396 2579
Fragments arranged by size:
2579 1396 1211 969 51 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4342 5207 5505 4342
Size: 865 298 5054
Fragments arranged by size:
5054 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3099 3155 4880 882
Size: 617 1600 56 1725 2219
Fragments arranged by size:
2219 1725 1600 617 56
BsaAI yAC'GTr
Cuts at: 493 1204 3504 4008 493
Size: 711 2300 504 2702
Fragments arranged by size:
2702 2300 711 504
BsaBI GATnn'nnATC
Cuts at: 3371 6211 3371
Size: 2840 3377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2223 2306 3439 4024
Size: 53 83 186 936 690 83 1133 585
Cuts at: 4024 4406 5041 275
Size: 382 635 1451
Fragments arranged by size:
1451 1133 936 690 635 585 382 186
83 83 53
BsaWI w'CCGG_w
Cuts at: 3120 4650 5048 5322 5469 5880 3120
Size: 1530 398 274 147 411 3457
Fragments arranged by size:
3457 1530 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3185 3215 1178
Size: 30 165 30 1782 30 4180
Fragments arranged by size:
4180 1782 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3475 1186
Size: 2289 3928
BseYI C'CCAG_C
Cuts at: 2198 2275 2698 3021 3384 3391 5420 5916 2198
Size: 77 423 323 363 7 2029 496 2499
Fragments arranged by size:
2499 2029 496 423 363 323 77 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 2873 1163
Size: 573 633 504 4507
Fragments arranged by size:
4507 633 573 504
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2112 3667 4428 5456 1540
Size: 275 186 111 1555 761 1028 2301
Fragments arranged by size:
2301 1555 1028 761 275 186 111
BsiHKAI G_wGCw'C
Cuts at: 2373 3405 3476 4278 4363 5434 2373
Size: 1032 71 802 85 1071 3156
Fragments arranged by size:
3156 1071 1032 802 85 71
BsmI GAATG_Cn'
Cuts at: 3728 3821 3728
Size: 93 6124
BsmBI CGTCTCn'nnnn_
Cuts at: 2421 2421
Size: 6217
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2168 2246 2282 2373 2616 2852
Size: 399 306 72 78 36 91 243 236
Cuts at: 2852 2969 3198 3405 3476 3485 3536 4278 4363
Size: 117 229 207 71 9 51 742 85
Cuts at: 4363 5434 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 306 243 236 229
207 117 91 85 78 72 71 51
36 9
BspEI T'CCGG_A
Cuts at: 3120 5880 3120
Size: 2760 3457
BspHI T'CATG_A
Cuts at: 3426 4106 5836 3426
Size: 680 1730 3807
Fragments arranged by size:
3807 1730 680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2866 2962 3123 3572 844
Size: 2022 96 161 449 3489
Fragments arranged by size:
3489 2022 449 161 96
BsrBI CCG'CTC
Cuts at: 1394 1823 2464 2944 4104 1394
Size: 429 641 480 1160 3507
Fragments arranged by size:
3507 1160 641 480 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4712 4886 65
Size: 1183 3464 174 1396
Fragments arranged by size:
3464 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 2111 2361 2920 3090 4861 5048
Size: 110 297 4 250 559 170 1771 187
Cuts at: 5048 1700
Size: 2869
Fragments arranged by size:
2869 1771 559 297 250 187 170 110
4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2185 2406 2740 3082 794
Size: 1391 221 334 342 3929
Fragments arranged by size:
3929 1391 342 334 221
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 2323 1184
Size: 1139 5078
BstXI CCAn_nnnn'nTGG
Cuts at: 2698 3016 3135 2698
Size: 318 119 5780
Fragments arranged by size:
5780 318 119
BstYI r'GATC_y
Cuts at: 1972 3547 3914 4299 4316 5768 5854 5866 6212
Size: 1575 367 385 17 1452 86 12 346
Cuts at: 6212 1972
Size: 1977
Fragments arranged by size:
1977 1575 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2218 2319 2818 2839 3134 513
Size: 552 1153 101 499 21 295 3596
Fragments arranged by size:
3596 1153 552 499 295 101 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 2128 2802 3733 4527 4547 2128
Size: 674 931 794 20 3798
Fragments arranged by size:
3798 931 794 674 20
ClaI AT'CG_AT
Cuts at: 1554 2029 1554
Size: 475 5742
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2190 2316
Size: 54 1425 50 275 18 279 81 126
Cuts at: 2316 2619 2685 2862 2945 2976 3664 4553 6182
Size: 303 66 177 83 31 688 889 1629
Cuts at: 6182 8
Size: 43
Fragments arranged by size:
1629 1425 889 688 303 279 275 177
126 83 81 66 54 50 43 31
18
EagI C'GGCC_G
Cuts at: 1537 1812 2109 3664 1537
Size: 275 297 1555 4090
Fragments arranged by size:
4090 1555 297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3332 4147 1353
Size: 1979 815 3423
Fragments arranged by size:
3423 1979 815
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2238 2409 2745 3205 4804 5178
Size: 1064 138 649 171 336 460 1599 374
Cuts at: 5178 5324 387
Size: 146 1280
Fragments arranged by size:
1599 1280 1064 649 460 374 336 171
146 138
EcoICRI GAG'CTC
Cuts at: 2371 3403 2371
Size: 1032 5185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 3062 3173 3419 3470 3481 4280
Size: 393 818 612 111 246 51 11 799
Cuts at: 4280 4877 5664 5932 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 818 799 787 612 597 393 268
246 111 51 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3169 3169
Size: 6217
EcoO109I rG'GnC_Cy
Cuts at: 2164 2242 2243 2164
Size: 78 1 6138
Fragments arranged by size:
6138 78 1
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FseI GG_CCGG'CC
Cuts at: 2111 2111
Size: 6217
FspI TGC'GCA
Cuts at: 3075 4723 3075
Size: 1648 4569
HaeII r_GCGC'y
Cuts at: 1678 2226 2563 3049 3442 5364 1678
Size: 548 337 486 393 1922 2531
Fragments arranged by size:
2531 1922 548 486 393 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4858 4890 4932 4964 1373
Size: 32 135 32 3286 32 42 32 2626
Fragments arranged by size:
3286 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3561 3807 669
Size: 988 281 1623 246 3079
Fragments arranged by size:
3079 1623 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KasI G'GCGC_C
Cuts at: 2222 3438 2222
Size: 1216 5001
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2282 3135 3337 5331 5515 1305
Size: 977 853 202 1994 184 2007
Fragments arranged by size:
2007 1994 977 853 202 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2192 2978 10
Size: 54 1425 343 360 786 3249
Fragments arranged by size:
3249 1425 786 360 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2330 2399 2432 2594 2807 4175 4534
Size: 648 1164 69 33 162 213 1368 359
Cuts at: 4534 4693 518
Size: 159 2042
Fragments arranged by size:
2042 1368 1164 648 359 213 162 159
69 33
MspA1I CmG'CkG
Cuts at: 1606 2220 2228 2291 2300 2383 2473 2580 2683
Size: 614 8 63 9 83 90 107 103
Cuts at: 2683 2698 2820 2841 2992 3035 3391 3633 4310
Size: 15 122 21 151 43 356 242 677
Cuts at: 4310 5458 5703 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 614 356 245 242 151
122 107 103 90 83 63 43 21
15 9 8
NaeI GCC'GGC
Cuts at: 2109 2113 2109
Size: 4 6213
NarI GG'CG_CC
Cuts at: 2223 3439 2223
Size: 1216 5001
NcoI C'CATG_G
Cuts at: 513 1065 2319 513
Size: 552 1254 4411
Fragments arranged by size:
4411 1254 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NgoMIV G'CCGG_C
Cuts at: 2107 2111 2107
Size: 4 6213
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NruI TCG'CGA
Cuts at: 2940 2940
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 2404 3214 3575 5120 1132
Size: 97 1175 810 361 1545 2229
Fragments arranged by size:
2229 1545 1175 810 361 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6217
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2275 3391 1108
Size: 1167 1116 3934
Fragments arranged by size:
3934 1167 1116
PfoI T'CCnGG_A
Cuts at: 2343 2343
Size: 6217
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PmlI CAC'GTG
Cuts at: 3504 3504
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 4286 4318 5799 5831 3185
Size: 32 1069 32 1481 32 3571
Fragments arranged by size:
3571 1481 1069 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PspOMI G'GGCC_C
Cuts at: 2164 2242 2164
Size: 78 6139
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2618 2650 2618
Size: 32 6185
PstI C_TGCA'G
Cuts at: 830 3569 830
Size: 2739 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2992 3391 2992
Size: 399 5818
RsrII CG'GwC_CG
Cuts at: 2753 2753
Size: 6217
SacI G_AGCT'C
Cuts at: 2373 3405 2373
Size: 1032 5185
SacII CC_GC'GG
Cuts at: 2221 2821 2842 2221
Size: 600 21 5596
Fragments arranged by size:
5596 600 21
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SfcI C'TryA_G
Cuts at: 826 1045 2635 3049 3565 4700 5381 5572 826
Size: 219 1590 414 516 1135 681 191 1471
Fragments arranged by size:
1590 1471 1135 681 516 414 219 191
SfoI GGC'GCC
Cuts at: 2224 3440 2224
Size: 1216 5001
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 2169 2898 3544 1872
Size: 297 729 646 4545
Fragments arranged by size:
4545 729 646 297
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4321 5222 5484 820
Size: 197 926 2378 901 262 1553
Fragments arranged by size:
2378 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SrfI GCCC'GGGC
Cuts at: 2169 2169
Size: 6217
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6217
StyI C'CwwG_G
Cuts at: 513 1065 2319 3656 513
Size: 552 1254 1337 3074
Fragments arranged by size:
3074 1337 1254 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2494 2947 4244 4261 4414 4599 5044
Size: 1255 914 453 1297 17 153 185 445
Cuts at: 5044 325
Size: 1498
Fragments arranged by size:
1498 1297 1255 914 453 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4463 6175 96
Size: 274 80 33 51 1443 2486 1712 138
Fragments arranged by size:
2486 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6177 6209 6177
Size: 32 6185
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 2167 2896 3542 1870
Size: 297 729 646 4545
Fragments arranged by size:
4545 729 646 297
XmnI GAAnn'nnTTC
Cuts at: 4346 4346
Size: 6217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BstZ17I Bsu36I DraIII EcoRV FalI FspAI NsiI PacI
PpuMI PspXI SanDI SapI SexAI SfiI SwaI XcmI