Restriction map for
pFN21AE3422
(Circular) (Six-base) MAPSORT of: pFN21AE3422.seq Check: 133 from: 1 to: 6184
pFN21AE3422 6184 bp
With 182 enzymes: SgfI *
March 28, 2012 12:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3994 278
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3527 1937
Size: 1590 4594
Acc65I G'GTAC_C
Cuts at: 2232 3505 2232
Size: 1273 4911
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2796 4247 5631 5899 1632
Size: 1164 1451 1384 268 1917
Fragments arranged by size:
1917 1451 1384 1164 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5987
AflIII A'CryG_T
Cuts at: 1203 5083 6060 1203
Size: 3880 977 1327
Fragments arranged by size:
3880 1327 977
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 4913 4913
Size: 6184
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2818 1184
Size: 1634 4550
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 6152
AlwNI CAG_nnn'CTG
Cuts at: 2465 3455 3927 5499 2465
Size: 990 472 1572 3150
Fragments arranged by size:
3150 1572 990 472
ApaLI G'TGCA_C
Cuts at: 4241 5397 4241
Size: 1156 5028
ApoI r'AATT_y
Cuts at: 1493 2246 3493 3719 3871 5980 1493
Size: 753 1247 226 152 2109 1697
Fragments arranged by size:
2109 1697 1247 753 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2337 3282 3405 4738 160
Size: 872 1305 945 123 1333 1606
Fragments arranged by size:
1606 1333 1305 945 872 123
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 3509 1870
Size: 73 1566 4545
Fragments arranged by size:
4545 1566 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2216 2249 3405 3438 2216
Size: 33 1156 33 4962
Fragments arranged by size:
4962 1156 33 33
BamHI G'GATC_C
Cuts at: 3514 3881 3514
Size: 367 5817
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2232 3505 4960 618
Size: 325 217 627 445 1273 1455 1842
Fragments arranged by size:
1842 1455 1273 627 445 325 217
BanII G_rGCy'C
Cuts at: 1391 2283 2512 2847 3503 1391
Size: 892 229 335 656 4072
Fragments arranged by size:
4072 892 656 335 229
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4375 4409 1903
Size: 34 2438 34 3678
Fragments arranged by size:
3678 2438 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3019 4076 5286 3019
Size: 1057 1210 3917
Fragments arranged by size:
3917 1210 1057
BclI T'GATC_A
Cuts at: 1548 2629 1548
Size: 1081 5103
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4795 136
Size: 107 122 71 631 605 3123 1525
Fragments arranged by size:
3123 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6179 6179
Size: 6184
BlpI GC'TnA_GC
Cuts at: 2841 3601 2841
Size: 760 5424
Bme1580I G_kGCm'C
Cuts at: 1790 4245 5401 1790
Size: 2455 1156 2573
Fragments arranged by size:
2573 2455 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6184
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2404 3915 4873
Size: 416 53 83 399 24 987 1511 958
Cuts at: 4873 442
Size: 1753
Fragments arranged by size:
1753 1511 987 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2496 2634 2733 4844 1239
Size: 1257 138 99 2111 2579
Fragments arranged by size:
2579 2111 1257 138 99
Bpu10I CC'TnA_GC
Cuts at: 2766 2766
Size: 6184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4309 5174 5472 4309
Size: 865 298 5021
Fragments arranged by size:
5021 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4847 882
Size: 617 3348 2219
Fragments arranged by size:
3348 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3975 493
Size: 711 2771 2702
Fragments arranged by size:
2771 2702 711
BsaBI GATnn'nnATC
Cuts at: 2628 6178 2628
Size: 3550 2634
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3991 4373 5008 275
Size: 53 83 186 936 2458 382 635 1451
Fragments arranged by size:
2458 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4617 5015 5289 5436 5847 4617
Size: 398 274 147 411 4954
Fragments arranged by size:
4954 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5959
Fragments arranged by size:
5959 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6184
BseYI C'CCAG_C
Cuts at: 3275 5387 5883 3275
Size: 2112 496 3576
Fragments arranged by size:
3576 2112 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5611
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3634 4395 5423 1540
Size: 275 186 1633 761 1028 2301
Fragments arranged by size:
2301 1633 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2283 2512 2847 4245 4330 5401 2283
Size: 229 335 1398 85 1071 3066
Fragments arranged by size:
3066 1398 1071 335 229 85
BsmI GAATG_Cn'
Cuts at: 3695 3788 3695
Size: 93 6091
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2512 2847 3503 4245 4330 5401
Size: 399 493 229 335 656 742 85 1071
Cuts at: 5401 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 656 493 399 335 229
85
BspEI T'CCGG_A
Cuts at: 5847 5847
Size: 6184
BspHI T'CATG_A
Cuts at: 4073 5803 4073
Size: 1730 4454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3539 844
Size: 2695 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4071 1394
Size: 429 2248 3507
Fragments arranged by size:
3507 2248 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2154 3205 4679 4853 65
Size: 1183 906 1051 1474 174 1396
Fragments arranged by size:
1474 1396 1183 1051 906 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4828 5015 1700
Size: 110 3018 187 2869
Fragments arranged by size:
3018 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6184
BssSI C'ACGA_G
Cuts at: 4244 5256 4244
Size: 1012 5172
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6184
BstBI TT'CG_AA
Cuts at: 4988 4988
Size: 6184
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6184
BstYI r'GATC_y
Cuts at: 1972 3514 3881 4266 4283 5735 5821 5833 6179
Size: 1542 367 385 17 1452 86 12 346
Cuts at: 6179 1972
Size: 1977
Fragments arranged by size:
1977 1542 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4693
Fragments arranged by size:
4693 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5445
Fragments arranged by size:
5445 683 56
BtsI GCAGTG_nn'
Cuts at: 3700 4494 4514 3700
Size: 794 20 5370
Fragments arranged by size:
5370 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6149
DraI TTT'AAA
Cuts at: 3067 3488 3067
Size: 421 5763
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5191 809
Size: 507 3875 1802
Fragments arranged by size:
3875 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 3631 4520
Size: 54 1425 50 275 18 264 1537 889
Cuts at: 4520 6149 8
Size: 1629 43
Fragments arranged by size:
1629 1537 1425 889 275 264 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3631 1537
Size: 275 1819 4090
Fragments arranged by size:
4090 1819 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4114 1353
Size: 2761 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4771 5145 5291 387
Size: 1064 138 3182 374 146 1280
Fragments arranged by size:
3182 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2281 2510 2845 2281
Size: 229 335 5620
Fragments arranged by size:
5620 335 229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2496 2634 2733 2796 4247 4844 5631
Size: 393 864 138 99 63 1451 597 787
Cuts at: 5631 5899 1239
Size: 268 1524
Fragments arranged by size:
1524 1451 864 787 597 393 268 138
99 63
EcoO109I rG'GnC_Cy
Cuts at: 3428 3428
Size: 6184
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 2821 2125
Size: 696 5488
FspI TGC'GCA
Cuts at: 4690 4690
Size: 6184
HaeII r_GCGC'y
Cuts at: 1678 5331 1678
Size: 3653 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4825 4857 4899 4931 1373
Size: 32 135 32 3253 32 42 32 2626
Fragments arranged by size:
3253 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2254 3528 3774 669
Size: 988 281 316 1274 246 3079
Fragments arranged by size:
3079 1274 988 316 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6184
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KpnI G_GTAC'C
Cuts at: 2236 3509 2236
Size: 1273 4911
MfeI C'AATT_G
Cuts at: 3783 3783
Size: 6184
MluI A'CGCG_T
Cuts at: 6060 6060
Size: 6184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2014 5298 5482 1305
Size: 709 3284 184 2007
Fragments arranged by size:
3284 2007 709 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 10
Size: 54 1425 343 264 4098
Fragments arranged by size:
4098 1425 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3407 4142 4501 4660 518
Size: 648 2241 735 359 159 2042
Fragments arranged by size:
2241 2042 735 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3132 3600 4277 5425 5670 1606
Size: 1526 468 677 1148 245 2120
Fragments arranged by size:
2120 1526 1148 677 468 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4693
Fragments arranged by size:
4693 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6184
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6184
NotI GC'GGCC_GC
Cuts at: 3631 3631
Size: 6184
NspI r_CATG'y
Cuts at: 1132 1229 3542 5087 1132
Size: 97 2313 1545 2229
Fragments arranged by size:
2313 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6184
PciI A'CATG_T
Cuts at: 5083 5083
Size: 6184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6184
PfoI T'CCnGG_A
Cuts at: 2611 2611
Size: 6184
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4253 4285 5766 5798 4253
Size: 32 1481 32 4639
Fragments arranged by size:
4639 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3428 3428
Size: 6184
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6184
PsiI TTA'TAA
Cuts at: 3057 3399 3754 3057
Size: 342 355 5487
Fragments arranged by size:
5487 355 342
PstI C_TGCA'G
Cuts at: 830 3197 3268 3536 830
Size: 2367 71 268 3478
Fragments arranged by size:
3478 2367 268 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
PvuII CAG'CTG
Cuts at: 3132 3132
Size: 6184
SacI G_AGCT'C
Cuts at: 2283 2512 2847 2283
Size: 229 335 5620
Fragments arranged by size:
5620 335 229
SalI G'TCGA_C
Cuts at: 1936 3526 1936
Size: 1590 4594
SbfI CC_TGCA'GG
Cuts at: 3536 3536
Size: 6184
ScaI AGT'ACT
Cuts at: 4432 6144 4432
Size: 1712 4472
SfcI C'TryA_G
Cuts at: 826 1045 2903 3193 3264 3532 4667 5348 5539
Size: 219 1858 290 71 268 1135 681 191
Cuts at: 5539 826
Size: 1471
Fragments arranged by size:
1858 1471 1135 681 290 268 219 191
71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6184
SmaI CCC'GGG
Cuts at: 1872 3511 1872
Size: 1639 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4288 5189 5451 820
Size: 197 926 2345 901 262 1553
Fragments arranged by size:
2345 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3975 493
Size: 3482 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 3542 1132
Size: 2410 3774
SspI AAT'ATT
Cuts at: 5 52 4108 5
Size: 47 4056 2081
Fragments arranged by size:
4056 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6184
StyI C'CwwG_G
Cuts at: 513 1065 2004 2672 3623 513
Size: 552 939 668 951 3074
Fragments arranged by size:
3074 951 939 668 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4211 4228 4381 4566 5011 325
Size: 1255 2631 17 153 185 445 1498
Fragments arranged by size:
2631 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2914 4430 6142
Size: 274 80 33 51 1443 937 1516 1712
Cuts at: 6142 96
Size: 138
Fragments arranged by size:
1712 1516 1443 937 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2076 2108 3208 3240 6144 6176 2076
Size: 32 1100 32 2904 32 2084
Fragments arranged by size:
2904 2084 1100 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6184
XbaI T'CTAG_A
Cuts at: 3520 3520
Size: 6184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2572 2572
Size: 6184
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6184
XmaI C'CCGG_G
Cuts at: 1870 3509 1870
Size: 1639 4545
XmnI GAAnn'nnTTC
Cuts at: 2935 4313 2935
Size: 1378 4806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3992 276
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI