Restriction map for
pFN21AE3413
(Circular) (Six-base) MAPSORT of: pFN21AE3413.seq Check: 5604 from: 1 to: 5743
pFN21AE3413 5743 bp
With 182 enzymes: SgfI *
March 28, 2012 12:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2728 2728
Size: 5743
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2775 3553 278
Size: 53 83 186 936 1239 778 2468
Fragments arranged by size:
2468 1239 936 778 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 1937
Size: 1149 4594
Acc65I G'GTAC_C
Cuts at: 2508 3064 2508
Size: 556 5187
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 3806 5190 5458 1632
Size: 593 1581 1384 268 1917
Fragments arranged by size:
1917 1581 1384 593 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 4642 5619 1203
Size: 3439 977 1327
Fragments arranged by size:
3439 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2149 2335 3486 5058 2149
Size: 186 1151 1572 2834
Fragments arranged by size:
2834 1572 1151 186
ApaI G_GGCC'C
Cuts at: 2955 2955
Size: 5743
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 3052 3278 3430 5539 1493
Size: 1559 226 152 2109 1697
Fragments arranged by size:
2109 1697 1559 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2451 2519 2617 3068 1870
Size: 73 508 68 98 451 4545
Fragments arranged by size:
4545 508 451 98 73 68
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2508 2534 2666 2740
Size: 325 217 627 255 466 26 132 74
Cuts at: 2740 3064 4519 618
Size: 324 1455 1842
Fragments arranged by size:
1842 1455 627 466 325 324 255 217
132 74 26
BanII G_rGCy'C
Cuts at: 1391 2518 2526 2697 2955 3062 1391
Size: 1127 8 171 258 107 4072
Fragments arranged by size:
4072 1127 258 171 107 8
BbeI G_GCGC'C
Cuts at: 2046 2046
Size: 5743
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2050 928
Size: 971 151 4621
Fragments arranged by size:
4621 971 151
BbvCI CC'TCA_GC
Cuts at: 2913 2913
Size: 5743
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2075 2109 3934 3968 1903
Size: 34 138 34 1825 34 3678
Fragments arranged by size:
3678 1825 138 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5743
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2939 4354 136
Size: 107 122 71 631 605 1267 1415 1525
Fragments arranged by size:
1525 1415 1267 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2721 5738 2721
Size: 3017 2726
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 2539 2669 2745 2955 3804 4960 1790
Size: 749 130 76 210 849 1156 2573
Fragments arranged by size:
2573 1156 849 749 210 130 76
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3474 4432 442
Size: 416 53 83 399 24 2057 958 1753
Fragments arranged by size:
2057 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2774 3056 4403 1239
Size: 1535 282 1347 2579
Fragments arranged by size:
2579 1535 1347 282
Bpu10I CC'TnA_GC
Cuts at: 2913 2913
Size: 5743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2084 2324 2411 2664 3011 3868 4733 5031 2084
Size: 240 87 253 347 857 865 298 2796
Fragments arranged by size:
2796 865 857 347 298 253 240 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4406 882
Size: 617 2907 2219
Fragments arranged by size:
2907 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2074 2977 3534 493
Size: 711 870 903 557 2702
Fragments arranged by size:
2702 903 870 711 557
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 2556 2772 3550
Size: 53 83 186 936 510 513 216 778
Cuts at: 3550 3932 4567 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 778 635 513 510 382 216
186 83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2220 2250 2708 2738 2987
Size: 30 165 30 817 30 458 30 249
Cuts at: 2987 3017 1178
Size: 30 3904
Fragments arranged by size:
3904 817 458 249 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2771 2811 2843 3008 1186
Size: 1585 40 32 165 3921
Fragments arranged by size:
3921 1585 165 40 32
BseYI C'CCAG_C
Cuts at: 2797 4946 5442 2797
Size: 2149 496 3098
Fragments arranged by size:
3098 2149 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2526 2697 3804 3889 4960 2526
Size: 171 1107 85 1071 3309
Fragments arranged by size:
3309 1107 1071 171 85
BsmI GAATG_Cn'
Cuts at: 3254 3347 3254
Size: 93 5650
BsmBI CGTCTCn'nnnn_
Cuts at: 2779 2779
Size: 5743
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2518 2526 2539 2669 2697 2745 2955
Size: 399 728 8 13 130 28 48 210
Cuts at: 2955 3062 3804 3889 4960 1391
Size: 107 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 728 399 210 130 107
85 48 28 13 8
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 3632 5362 3632
Size: 1730 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2334 2401 2409 2728 2938 3098 844
Size: 1490 67 8 319 210 160 3489
Fragments arranged by size:
3489 1490 319 210 160 67 8
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4238 4412 65
Size: 1183 2990 174 1396
Fragments arranged by size:
2990 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2982 4387 4574 1700
Size: 110 1172 1405 187 2869
Fragments arranged by size:
2869 1405 1172 187 110
BsrGI T'GTAC_A
Cuts at: 96 2374 96
Size: 2278 3465
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 3803 4815 3803
Size: 1012 4731
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2335 794
Size: 1541 4202
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstXI CCAn_nnnn'nTGG
Cuts at: 2074 2752 2074
Size: 678 5065
BstYI r'GATC_y
Cuts at: 1972 2263 2721 2791 3073 3440 3825 3842 5294
Size: 291 458 70 282 367 385 17 1452
Cuts at: 5294 5380 5392 5738 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 458 385 367 346 291 282
86 70 17 12
Bsu36I CC'TnA_GG
Cuts at: 2114 2416 2114
Size: 302 5441
BtgI C'CryG_G
Cuts at: 513 1065 2004 2047 513
Size: 552 939 43 4209
Fragments arranged by size:
4209 939 552 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2818 487
Size: 683 56 1592 3412
Fragments arranged by size:
3412 1592 683 56
BtsI GCAGTG_nn'
Cuts at: 3259 4053 4073 3259
Size: 794 20 4929
Fragments arranged by size:
4929 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DraIII CAC_nnn'GTG
Cuts at: 2225 2494 2505 2225
Size: 269 11 5463
Fragments arranged by size:
5463 269 11
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2555 4750 809
Size: 507 1239 2195 1802
Fragments arranged by size:
2195 1802 1239 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2268 2496 2687
Size: 54 1425 50 275 18 438 228 191
Cuts at: 2687 3190 4079 5708 8
Size: 503 889 1629 43
Fragments arranged by size:
1629 1425 889 503 438 275 228 191
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3673 1353
Size: 2320 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4330 4704 4850 387
Size: 1064 138 2741 374 146 1280
Fragments arranged by size:
2741 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2524 2695 2524
Size: 171 5572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2774 3056 3806 4403 5190 5458
Size: 393 593 549 282 750 597 787 268
Cuts at: 5458 1239
Size: 1524
Fragments arranged by size:
1524 787 750 597 593 549 393 282
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2102 2756 2102
Size: 654 5089
EcoO109I rG'GnC_Cy
Cuts at: 2146 2383 2604 2927 2951 2952 2146
Size: 237 221 323 24 1 4937
Fragments arranged by size:
4937 323 237 221 24 1
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2743 2743
Size: 5743
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 2046 2415 4890 1678
Size: 368 369 2475 2531
Fragments arranged by size:
2531 2475 369 368
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2149 2181 2552 2584 4384
Size: 32 135 32 577 32 371 32 1800
Cuts at: 4384 4416 4458 4490 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1800 577 371 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3087 3333 669
Size: 988 281 1149 246 3079
Fragments arranged by size:
3079 1149 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KasI G'GCGC_C
Cuts at: 2042 2042
Size: 5743
KpnI G_GTAC'C
Cuts at: 2512 3068 2512
Size: 556 5187
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2103 2241 4857 5041 1305
Size: 798 138 2616 184 2007
Fragments arranged by size:
2616 2007 798 184 138
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2498 10
Size: 54 1425 343 666 3255
Fragments arranged by size:
3255 1425 666 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2964 3701 4060 4219 518
Size: 648 1798 737 359 159 2042
Fragments arranged by size:
2042 1798 737 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3159 3836 4984 5229 1606
Size: 1553 677 1148 245 2120
Fragments arranged by size:
2120 1553 1148 677 245
NarI GG'CG_CC
Cuts at: 2043 2043
Size: 5743
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4252
Fragments arranged by size:
4252 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 3101 4646 1132
Size: 97 1872 1545 2229
Fragments arranged by size:
2229 1872 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 4642 4642
Size: 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2225 2505 2788 1108
Size: 1117 280 283 4063
Fragments arranged by size:
4063 1117 283 280
PfoI T'CCnGG_A
Cuts at: 3033 3033
Size: 5743
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PmlI CAC'GTG
Cuts at: 2074 2977 2074
Size: 903 4840
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2383 2604 2927 2383
Size: 221 323 5199
Fragments arranged by size:
5199 323 221
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PspOMI G'GGCC_C
Cuts at: 2951 2951
Size: 5743
PspXI vC'TCGA_Gb
Cuts at: 2451 2451
Size: 5743
PstI C_TGCA'G
Cuts at: 830 2172 2331 3095 830
Size: 1342 159 764 3478
Fragments arranged by size:
3478 1342 764 159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
SacI G_AGCT'C
Cuts at: 2526 2697 2526
Size: 171 5572
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SanDI GG'GwC_CC
Cuts at: 2604 2604
Size: 5743
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 2015 2864 3991 5703 2015
Size: 849 1127 1712 2055
Fragments arranged by size:
2055 1712 1127 849
SexAI A'CCwGG_T
Cuts at: 2490 2501 2490
Size: 11 5732
SfcI C'TryA_G
Cuts at: 826 1045 2168 2327 3091 4226 4907 5098 826
Size: 219 1123 159 764 1135 681 191 1471
Fragments arranged by size:
1471 1135 1123 764 681 219 191 159
SfoI GGC'GCC
Cuts at: 2044 2044
Size: 5743
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 2619 3070 1872
Size: 747 451 4545
Fragments arranged by size:
4545 747 451
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2099 2339 2426 2451 2679 2990
Size: 197 926 156 240 87 25 228 311
Cuts at: 2990 3847 4748 5010 820
Size: 857 901 262 1553
Fragments arranged by size:
1553 926 901 857 311 262 240 228
197 156 87 25
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 3667 5
Size: 47 3615 2081
Fragments arranged by size:
3615 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5743
StyI C'CwwG_G
Cuts at: 513 1065 2004 2844 2937 3182 513
Size: 552 939 840 93 245 3074
Fragments arranged by size:
3074 939 840 552 245 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3770 3787 3940 4125 4570 325
Size: 1255 2190 17 153 185 445 1498
Fragments arranged by size:
2190 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2013 2374 2862
Size: 274 80 33 51 1443 36 361 488
Cuts at: 2862 3989 5701 96
Size: 1127 1712 138
Fragments arranged by size:
1712 1443 1127 488 361 274 138 80
51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2220 2252 5703 5735 2220
Size: 32 3451 32 2228
Fragments arranged by size:
3451 2228 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 2451 1943
Size: 508 5235
XmaI C'CCGG_G
Cuts at: 1870 2617 3068 1870
Size: 747 451 4545
Fragments arranged by size:
4545 747 451
XmnI GAAnn'nnTTC
Cuts at: 3872 3872
Size: 5743
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2773 3551 276
Size: 53 83 186 936 1239 778 2468
Fragments arranged by size:
2468 1239 936 778 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI
SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PsrI PvuII RsrII SacII SapI SfiI SrfI
SwaI XcmI