Restriction map for
pFN21AE3410
(Circular) (Six-base) MAPSORT of: pFN21AE3410.seq Check: 891 from: 1 to: 5635
pFN21AE3410 5635 bp
With 182 enzymes: SgfI *
March 28, 2012 12:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2107 2107
Size: 5635
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2628 3445 278
Size: 53 83 186 936 1092 817 2468
Fragments arranged by size:
2468 1092 936 817 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2978 1937
Size: 1041 4594
Acc65I G'GTAC_C
Cuts at: 2956 2956
Size: 5635
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3698 5082 5350 1632
Size: 2066 1384 268 1917
Fragments arranged by size:
2066 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 4534 5511 1203
Size: 3331 977 1327
Fragments arranged by size:
3331 1327 977
AgeI A'CCGG_T
Cuts at: 2893 4466 2893
Size: 1573 4062
AhdI GACnn_n'nnGTC
Cuts at: 2607 4364 2607
Size: 1757 3878
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5635
AlwNI CAG_nnn'CTG
Cuts at: 2527 3378 4950 2527
Size: 851 1572 3212
Fragments arranged by size:
3212 1572 851
ApaLI G'TGCA_C
Cuts at: 3692 4848 3692
Size: 1156 4479
ApoI r'AATT_y
Cuts at: 1493 2944 3170 3322 5431 1493
Size: 1451 226 152 2109 1697
Fragments arranged by size:
2109 1697 1451 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2960 1870
Size: 73 1017 4545
Fragments arranged by size:
4545 1017 73
BamHI G'GATC_C
Cuts at: 2965 3332 2965
Size: 367 5268
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2956 4411 618
Size: 325 217 627 1169 1455 1842
Fragments arranged by size:
1842 1455 1169 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2132 2508 2954 1391
Size: 741 376 446 4072
Fragments arranged by size:
4072 741 446 376
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3826 3860 1903
Size: 34 1889 34 3678
Fragments arranged by size:
3678 1889 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 4246 136
Size: 107 122 71 631 605 343 2231 1525
Fragments arranged by size:
2231 1525 631 605 343 122 107 71
BglII A'GATC_T
Cuts at: 5630 5630
Size: 5635
BlpI GC'TnA_GC
Cuts at: 3052 3052
Size: 5635
Bme1580I G_kGCm'C
Cuts at: 1790 2572 3696 4852 1790
Size: 782 1124 1156 2573
Fragments arranged by size:
2573 1156 1124 782
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5635
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3366 4324 442
Size: 416 53 83 399 24 1949 958 1753
Fragments arranged by size:
1949 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2720 2814 4295 1239
Size: 1481 94 1481 2579
Fragments arranged by size:
2579 1481 1481 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3760 4625 4923 3760
Size: 865 298 4472
Fragments arranged by size:
4472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2349 4298 882
Size: 617 850 1949 2219
Fragments arranged by size:
2219 1949 850 617
BsaAI yAC'GTr
Cuts at: 493 1204 3426 493
Size: 711 2222 2702
Fragments arranged by size:
2702 2222 711
BsaBI GATnn'nnATC
Cuts at: 2088 2357 5629 2088
Size: 269 3272 2094
Fragments arranged by size:
3272 2094 269
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2625 3442 3824 4459
Size: 53 83 186 936 1092 817 382 635
Cuts at: 4459 275
Size: 1451
Fragments arranged by size:
1451 1092 936 817 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2042 2371 2893 4068 4466 4740 4887 5298 2042
Size: 329 522 1175 398 274 147 411 2379
Fragments arranged by size:
2379 1175 522 411 398 329 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2353 2383 1178
Size: 30 165 30 950 30 4430
Fragments arranged by size:
4430 950 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2119 2358 2876 1186
Size: 933 239 518 3945
Fragments arranged by size:
3945 933 518 239
BseYI C'CCAG_C
Cuts at: 2027 2262 2444 4838 5334 2027
Size: 235 182 2394 496 2328
Fragments arranged by size:
2394 2328 496 235 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5062
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2894 3085 3846 4874 1540
Size: 275 186 893 191 761 1028 2301
Fragments arranged by size:
2301 1028 893 761 275 191 186
BsiHKAI G_wGCw'C
Cuts at: 2132 2250 3696 3781 4852 2132
Size: 118 1446 85 1071 2915
Fragments arranged by size:
2915 1446 1071 118 85
BsmI GAATG_Cn'
Cuts at: 3146 3239 3146
Size: 93 5542
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2132 2250 2508 2572 2954 3696 3781
Size: 399 342 118 258 64 382 742 85
Cuts at: 3781 4852 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 382 342 258 118
85 64
BspEI T'CCGG_A
Cuts at: 2042 5298 2042
Size: 3256 2379
BspHI T'CATG_A
Cuts at: 3524 5254 3524
Size: 1730 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2107 2990 844
Size: 1263 883 3489
Fragments arranged by size:
3489 1263 883
BsrBI CCG'CTC
Cuts at: 1394 1823 2566 3522 1394
Size: 429 743 956 3507
Fragments arranged by size:
3507 956 743 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4130 4304 65
Size: 1183 2882 174 1396
Fragments arranged by size:
2882 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 2893 4279 4466 1700
Size: 110 223 860 1386 187 2869
Fragments arranged by size:
2869 1386 860 223 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5635
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5635
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5635
BstBI TT'CG_AA
Cuts at: 4439 4439
Size: 5635
BstEII G'GTnAC_C
Cuts at: 1184 2374 1184
Size: 1190 4445
BstYI r'GATC_y
Cuts at: 1972 2910 2965 3332 3717 3734 5186 5272 5284
Size: 938 55 367 385 17 1452 86 12
Cuts at: 5284 5630 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 938 385 367 346 86 55
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2175 513
Size: 552 939 171 3973
Fragments arranged by size:
3973 939 552 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2571 487
Size: 683 56 1345 3551
Fragments arranged by size:
3551 1345 683 56
BtsI GCAGTG_nn'
Cuts at: 2361 2406 3151 3945 3965 2361
Size: 45 745 794 20 4031
Fragments arranged by size:
4031 794 745 45 20
ClaI AT'CG_AT
Cuts at: 1554 2874 1554
Size: 1320 4315
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5600
DraI TTT'AAA
Cuts at: 2939 2939
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2522 4642 809
Size: 507 1206 2120 1802
Fragments arranged by size:
2120 1802 1206 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2019 2586
Size: 54 1425 50 275 18 177 12 567
Cuts at: 2586 3082 3971 5600 8
Size: 496 889 1629 43
Fragments arranged by size:
1629 1425 889 567 496 275 177 54
50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3082 1537
Size: 275 1270 4090
Fragments arranged by size:
4090 1270 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2688 2712 2775 3565 1353
Size: 1335 24 63 790 3423
Fragments arranged by size:
3423 1335 790 63 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4222 4596 4742 387
Size: 1064 138 2633 374 146 1280
Fragments arranged by size:
2633 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2130 2130
Size: 5635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2720 2814 3698 4295 5082 5350 1239
Size: 393 1088 94 884 597 787 268 1524
Fragments arranged by size:
1524 1088 884 787 597 393 268 94
EcoO109I rG'GnC_Cy
Cuts at: 2259 2401 2524 2259
Size: 142 123 5370
Fragments arranged by size:
5370 142 123
EcoRI G'AATT_C
Cuts at: 2944 3322 2944
Size: 378 5257
EcoRV GAT'ATC
Cuts at: 2281 2281
Size: 5635
FspI TGC'GCA
Cuts at: 4141 4141
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 4782 1678
Size: 3104 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2188 2495 2527 2593
Size: 32 135 32 584 32 307 32 66
Cuts at: 2593 2625 4276 4308 4350 4382 1373
Size: 32 1651 32 42 32 2626
Fragments arranged by size:
2626 1651 584 307 135 66 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2979 3225 669
Size: 988 281 1041 246 3079
Fragments arranged by size:
3079 1041 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5635
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KpnI G_GTAC'C
Cuts at: 2960 2960
Size: 5635
MfeI C'AATT_G
Cuts at: 3234 3234
Size: 5635
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2516 4749 4933 1305
Size: 1211 2233 184 2007
Fragments arranged by size:
2233 2007 1211 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2588 10
Size: 54 1425 343 756 3057
Fragments arranged by size:
3057 1425 756 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3593 3952 4111 518
Size: 648 2427 359 159 2042
Fragments arranged by size:
2427 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2027 3051 3728 4876 5121 1606
Size: 421 1024 677 1148 245 2120
Fragments arranged by size:
2120 1148 1024 677 421 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4144
Fragments arranged by size:
4144 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 3082 3082
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2993 4538 1132
Size: 97 1764 1545 2229
Fragments arranged by size:
2229 1764 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5635
PciI A'CATG_T
Cuts at: 4534 4534
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5635
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2259 2524 2259
Size: 265 5370
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2118 2202 2413 2826 2987 830
Size: 1288 84 211 413 161 3478
Fragments arranged by size:
3478 1288 413 211 161 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
PvuII CAG'CTG
Cuts at: 2027 2027
Size: 5635
SacI G_AGCT'C
Cuts at: 2132 2132
Size: 5635
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SapI GCTCTTCn'nnn_
Cuts at: 2688 2775 2688
Size: 87 5548
SbfI CC_TGCA'GG
Cuts at: 2118 2987 2118
Size: 869 4766
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SexAI A'CCwGG_T
Cuts at: 2520 2520
Size: 5635
SfcI C'TryA_G
Cuts at: 826 1045 2114 2198 2409 2822 2983 4118 4799
Size: 219 1069 84 211 413 161 1135 681
Cuts at: 4799 4990 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1069 681 413 219 211 191
161 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2962 1872
Size: 1090 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3739 4640 4902 820
Size: 197 926 1796 901 262 1553
Fragments arranged by size:
1796 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5635
StyI C'CwwG_G
Cuts at: 513 1065 2004 2397 3074 513
Size: 552 939 393 677 3074
Fragments arranged by size:
3074 939 677 552 393
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3662 3679 3832 4017 4462 325
Size: 1255 2082 17 153 185 445 1498
Fragments arranged by size:
2082 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3881 5593 96
Size: 274 80 33 51 1443 1904 1712 138
Fragments arranged by size:
1904 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2353 2385 5595 5627 2353
Size: 32 3210 32 2361
Fragments arranged by size:
3210 2361 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5635
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5635
XmaI C'CCGG_G
Cuts at: 1870 2960 1870
Size: 1090 4545
XmnI GAAnn'nnTTC
Cuts at: 3764 3764
Size: 5635
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2626 3443 276
Size: 53 83 186 936 1092 817 2468
Fragments arranged by size:
2468 1092 936 817 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI