Restriction map for
pFN21AE3409
(Circular) (Six-base) MAPSORT of: pFN21AE3409.seq Check: 4856 from: 1 to: 5620
pFN21AE3409 5620 bp
With 182 enzymes: SgfI *
March 28, 2012 12:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2963 1937
Size: 1026 4594
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2168 2363 2775 3683 5067 5335 1632
Size: 536 195 412 908 1384 268 1917
Fragments arranged by size:
1917 1384 908 536 412 268 195
AfeI AGC'GCT
Cuts at: 2060 2060
Size: 5620
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5620
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2078 2110 2078
Size: 32 5588
AlwNI CAG_nnn'CTG
Cuts at: 2080 2554 2809 2897 3363 4935 2080
Size: 474 255 88 466 1572 2765
Fragments arranged by size:
2765 1572 474 466 255 88
ApaLI G'TGCA_C
Cuts at: 2584 3677 4833 2584
Size: 1093 1156 3371
Fragments arranged by size:
3371 1156 1093
ApoI r'AATT_y
Cuts at: 1493 2929 3155 3307 5416 1493
Size: 1436 226 152 2109 1697
Fragments arranged by size:
2109 1697 1436 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 2945 1870
Size: 73 88 914 4545
Fragments arranged by size:
4545 914 88 73
AvrII C'CTAG_G
Cuts at: 2665 2665
Size: 5620
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2941 4396 618
Size: 325 217 627 1154 1455 1842
Fragments arranged by size:
1842 1455 1154 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2543 2605 2613 2939 1391
Size: 1152 62 8 326 4072
Fragments arranged by size:
4072 1152 326 62 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4649
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BfrBI ATG'CAT
Cuts at: 2209 2526 2209
Size: 317 5303
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 2274 3037 2274
Size: 763 4857
Bme1580I G_kGCm'C
Cuts at: 1790 2588 3681 4837 1790
Size: 798 1093 1156 2573
Fragments arranged by size:
2573 1156 1093 798
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2090 2578 3351
Size: 416 53 83 399 24 673 488 773
Cuts at: 3351 4309 442
Size: 958 1753
Fragments arranged by size:
1753 958 773 673 488 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2632 2632
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2894 4280 1239
Size: 1655 1386 2579
Fragments arranged by size:
2579 1655 1386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2432 3745 4610 4908 2432
Size: 1313 865 298 3144
Fragments arranged by size:
3144 1313 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4283 882
Size: 617 2784 2219
Fragments arranged by size:
2784 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3411 493
Size: 711 2207 2702
Fragments arranged by size:
2702 2207 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2108 1178
Size: 30 165 30 675 30 4690
Fragments arranged by size:
4690 675 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2849 2852 1186
Size: 1012 651 3 3954
Fragments arranged by size:
3954 1012 651 3
BseYI C'CCAG_C
Cuts at: 2190 2392 2577 2890 4823 5319 2190
Size: 202 185 313 1933 496 2491
Fragments arranged by size:
2491 1933 496 313 202 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2642 2771 1163
Size: 573 906 129 4012
Fragments arranged by size:
4012 906 573 129
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2588 2613 3681 3766 4837 2588
Size: 25 1068 85 1071 3371
Fragments arranged by size:
3371 1071 1068 85 25
BsmI GAATG_Cn'
Cuts at: 2785 3131 3224 2785
Size: 346 93 5181
Fragments arranged by size:
5181 346 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2543 2588 2605 2613 2939 3681 3766
Size: 399 753 45 17 8 326 742 85
Cuts at: 3766 4837 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 753 742 399 326 85 45
17 8
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 3509 5239 3509
Size: 1730 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 844
Size: 2131 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2647 3507 1394
Size: 429 824 860 3507
Fragments arranged by size:
3507 860 824 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2795 4115 4289 65
Size: 1183 1547 1320 174 1396
Fragments arranged by size:
1547 1396 1320 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 2165 2243 3680 4692 2165
Size: 78 1437 1012 3093
Fragments arranged by size:
3093 1437 1012 78
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstYI r'GATC_y
Cuts at: 1972 2397 2950 3317 3702 3719 5171 5257 5269
Size: 425 553 367 385 17 1452 86 12
Cuts at: 5269 5615 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 553 425 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2826 513
Size: 552 939 822 3307
Fragments arranged by size:
3307 939 822 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2195 487
Size: 683 56 969 3912
Fragments arranged by size:
3912 969 683 56
BtsI GCAGTG_nn'
Cuts at: 3136 3930 3950 3136
Size: 794 20 4806
Fragments arranged by size:
4806 794 20
ClaI AT'CG_AT
Cuts at: 1554 2640 1554
Size: 1086 4534
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2899 3067 3956
Size: 54 1425 50 275 18 1069 168 889
Cuts at: 3956 5585 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1069 889 275 168 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 2222 2618 3550 1353
Size: 719 150 396 932 3423
Fragments arranged by size:
3423 932 719 396 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2611 2611
Size: 5620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2363 2775 2894 3683 4280 5067
Size: 393 536 195 412 119 789 597 787
Cuts at: 5067 5335 1239
Size: 268 1524
Fragments arranged by size:
1524 789 787 597 536 412 393 268
195 119
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 2366
Size: 5620
EcoO109I rG'GnC_Cy
Cuts at: 2702 2702
Size: 5620
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 2062 2882 4767 1678
Size: 384 820 1885 2531
Fragments arranged by size:
2531 1885 820 384
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2356 2388 2645 2677 4261
Size: 32 135 32 784 32 257 32 1584
Cuts at: 4261 4293 4335 4367 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1584 784 257 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4734 4918 1305
Size: 3429 184 2007
Fragments arranged by size:
3429 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2901 10
Size: 54 1425 343 1069 2729
Fragments arranged by size:
2729 1425 1069 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2531 3578 3937 4096 518
Size: 648 1365 1047 359 159 2042
Fragments arranged by size:
2042 1365 1047 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2581 2872 3036 3713 4861 5106 1606
Size: 975 291 164 677 1148 245 2120
Fragments arranged by size:
2120 1148 975 677 291 245 164
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4129
Fragments arranged by size:
4129 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NsiI A_TGCA'T
Cuts at: 2211 2528 2211
Size: 317 5303
NspI r_CATG'y
Cuts at: 1132 1229 2209 2526 2978 4523 1132
Size: 97 980 317 452 1545 2229
Fragments arranged by size:
2229 1545 980 452 317 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2080 2897 1108
Size: 972 817 3831
Fragments arranged by size:
3831 972 817
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2078 2110 3689 3721 5202 5234 2078
Size: 32 1579 32 1481 32 2464
Fragments arranged by size:
2464 1579 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2654 1613
Size: 1041 4579
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2405 2565 2886 2972 830
Size: 1575 160 321 86 3478
Fragments arranged by size:
3478 1575 321 160 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
PvuII CAG'CTG
Cuts at: 2872 2872
Size: 5620
SacI G_AGCT'C
Cuts at: 2613 2613
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SapI GCTCTTCn'nnn_
Cuts at: 2618 2618
Size: 5620
SbfI CC_TGCA'GG
Cuts at: 2565 2972 2565
Size: 407 5213
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2401 2561 2882 2968 4103 4784 4975
Size: 219 1356 160 321 86 1135 681 191
Cuts at: 4975 826
Size: 1471
Fragments arranged by size:
1471 1356 1135 681 321 219 191 160
86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 3724 4625 4887 820
Size: 197 926 468 1313 901 262 1553
Fragments arranged by size:
1553 1313 926 901 468 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2526 2978 1132
Size: 1394 452 3774
Fragments arranged by size:
3774 1394 452
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 2285 2409 1517
Size: 768 124 4728
Fragments arranged by size:
4728 768 124
StyI C'CwwG_G
Cuts at: 513 1065 2004 2427 2665 3059 513
Size: 552 939 423 238 394 3074
Fragments arranged by size:
3074 939 552 423 394 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2381 3647 3664 3817 4002 4447 325
Size: 1255 801 1266 17 153 185 445 1498
Fragments arranged by size:
1498 1266 1255 801 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3866 5578 96
Size: 274 80 33 51 1443 1889 1712 138
Fragments arranged by size:
1889 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5580 5612 5580
Size: 32 5588
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI