Restriction map for
pFN21AE3407
(Circular) (Six-base) MAPSORT of: pFN21AE3407.seq Check: 960 from: 1 to: 5557
pFN21AE3407 5557 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3367 278
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2900 1937
Size: 963 4594
Acc65I G'GTAC_C
Cuts at: 2878 2878
Size: 5557
AclI AA'CG_TT
Cuts at: 1563 3684 4057 1563
Size: 2121 373 3063
Fragments arranged by size:
3063 2121 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2073 2774 3620 5004 5272 1632
Size: 441 701 846 1384 268 1917
Fragments arranged by size:
1917 1384 846 701 441 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5360
AflIII A'CryG_T
Cuts at: 1203 4456 5433 1203
Size: 3253 977 1327
Fragments arranged by size:
3253 1327 977
AgeI A'CCGG_T
Cuts at: 4388 4388
Size: 5557
AhdI GACnn_n'nnGTC
Cuts at: 4286 4286
Size: 5557
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5557
AlwNI CAG_nnn'CTG
Cuts at: 2187 2453 3300 4872 2187
Size: 266 847 1572 2872
Fragments arranged by size:
2872 1572 847 266
ApaLI G'TGCA_C
Cuts at: 3614 4770 3614
Size: 1156 4401
ApoI r'AATT_y
Cuts at: 1493 2798 2866 3092 3244 5353 1493
Size: 1305 68 226 152 2109 1697
Fragments arranged by size:
2109 1697 1305 226 152 68
AseI AT'TA_AT
Cuts at: 160 1032 4111 160
Size: 872 3079 1606
Fragments arranged by size:
3079 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
AvaI C'yCGr_G
Cuts at: 1870 1943 2882 1870
Size: 73 939 4545
Fragments arranged by size:
4545 939 73
BamHI G'GATC_C
Cuts at: 2845 2887 3254 2845
Size: 42 367 5148
Fragments arranged by size:
5148 367 42
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2878 4333 618
Size: 325 217 627 1091 1455 1842
Fragments arranged by size:
1842 1455 1091 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2876 1391
Size: 1485 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4586
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3748 3782 1903
Size: 34 1811 34 3678
Fragments arranged by size:
3678 1811 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3449 4659 3449
Size: 1210 4347
BclI T'GATC_A
Cuts at: 1548 2584 1548
Size: 1036 4521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4168 136
Size: 107 122 71 631 605 2496 1525
Fragments arranged by size:
2496 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5552 5552
Size: 5557
BlpI GC'TnA_GC
Cuts at: 2688 2974 2688
Size: 286 5271
Bme1580I G_kGCm'C
Cuts at: 1790 3618 4774 1790
Size: 1828 1156 2573
Fragments arranged by size:
2573 1828 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5557
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3288 4246 442
Size: 416 53 83 399 24 1871 958 1753
Fragments arranged by size:
1871 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4217 1239
Size: 2978 2579
Bpu10I CC'TnA_GC
Cuts at: 2597 2597
Size: 5557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3682 4547 4845 3682
Size: 865 298 4394
Fragments arranged by size:
4394 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2070 4220 882
Size: 617 571 2150 2219
Fragments arranged by size:
2219 2150 617 571
BsaAI yAC'GTr
Cuts at: 493 1204 3348 493
Size: 711 2144 2702
Fragments arranged by size:
2702 2144 711
BsaBI GATnn'nnATC
Cuts at: 5551 5551
Size: 5557
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3364 3746 4381 275
Size: 53 83 186 936 1831 382 635 1451
Fragments arranged by size:
1831 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3990 4388 4662 4809 5220 3990
Size: 398 274 147 411 4327
Fragments arranged by size:
4327 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5332
Fragments arranged by size:
5332 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2222 1186
Size: 889 147 4521
Fragments arranged by size:
4521 889 147
BseYI C'CCAG_C
Cuts at: 4760 5256 4760
Size: 496 5061
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4984
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3007 3768 4796 1540
Size: 275 186 1006 761 1028 2301
Fragments arranged by size:
2301 1028 1006 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3618 3703 4774 3618
Size: 85 1071 4401
Fragments arranged by size:
4401 1071 85
BsmI GAATG_Cn'
Cuts at: 3068 3161 3068
Size: 93 5464
BsmBI CGTCTCn'nnnn_
Cuts at: 2738 2738
Size: 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2876 3618 3703 4774 1391
Size: 399 1086 742 85 1071 2174
Fragments arranged by size:
2174 1086 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5220 5220
Size: 5557
BspHI T'CATG_A
Cuts at: 3446 5176 3446
Size: 1730 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2912 844
Size: 2068 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3444 1394
Size: 429 1621 3507
Fragments arranged by size:
3507 1621 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4052 4226 65
Size: 1183 2804 174 1396
Fragments arranged by size:
2804 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4201 4388 1700
Size: 110 2391 187 2869
Fragments arranged by size:
2869 2391 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5557
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5557
BssSI C'ACGA_G
Cuts at: 3617 4629 3617
Size: 1012 4545
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5557
BstBI TT'CG_AA
Cuts at: 4361 4361
Size: 5557
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5557
BstYI r'GATC_y
Cuts at: 1972 2140 2305 2845 2887 3254 3639 3656 5108
Size: 168 165 540 42 367 385 17 1452
Cuts at: 5108 5194 5206 5552 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 540 385 367 346 168 165
86 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4066
Fragments arranged by size:
4066 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4818
Fragments arranged by size:
4818 683 56
BtsI GCAGTG_nn'
Cuts at: 3073 3867 3887 3073
Size: 794 20 4743
Fragments arranged by size:
4743 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5522
DraI TTT'AAA
Cuts at: 2101 2605 2861 2101
Size: 504 256 4797
Fragments arranged by size:
4797 504 256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4564 809
Size: 507 3248 1802
Fragments arranged by size:
3248 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3004 3893 5522
Size: 54 1425 50 275 18 1174 889 1629
Cuts at: 5522 8
Size: 43
Fragments arranged by size:
1629 1425 1174 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3004 1537
Size: 275 1192 4090
Fragments arranged by size:
4090 1192 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 3487 1353
Size: 699 1435 3423
Fragments arranged by size:
3423 1435 699
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4144 4518 4664 387
Size: 1064 138 2555 374 146 1280
Fragments arranged by size:
2555 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2774 3620 4217 5004 5272 1239
Size: 393 441 701 846 597 787 268 1524
Fragments arranged by size:
1524 846 787 701 597 441 393 268
EcoRI G'AATT_C
Cuts at: 2866 3244 2866
Size: 378 5179
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2444 2444
Size: 5557
FspI TGC'GCA
Cuts at: 4063 4063
Size: 5557
HaeII r_GCGC'y
Cuts at: 1678 4704 1678
Size: 3026 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2201 2233 2837 2869 4198
Size: 32 135 32 629 32 604 32 1329
Cuts at: 4198 4230 4272 4304 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1329 629 604 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2247 2901 3147 669
Size: 988 281 309 654 246 3079
Fragments arranged by size:
3079 988 654 309 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5557
HpaI GTT'AAC
Cuts at: 2247 3147 2247
Size: 900 4657
KpnI G_GTAC'C
Cuts at: 2882 2882
Size: 5557
MfeI C'AATT_G
Cuts at: 3156 3156
Size: 5557
MluI A'CGCG_T
Cuts at: 5433 5433
Size: 5557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2254 4671 4855 1305
Size: 949 2417 184 2007
Fragments arranged by size:
2417 2007 949 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3735
Fragments arranged by size:
3735 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3515 3874 4033 518
Size: 648 2349 359 159 2042
Fragments arranged by size:
2349 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2489 2973 3650 4798 5043 1606
Size: 883 484 677 1148 245 2120
Fragments arranged by size:
2120 1148 883 677 484 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4066
Fragments arranged by size:
4066 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5557
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5557
NotI GC'GGCC_GC
Cuts at: 3004 3004
Size: 5557
NspI r_CATG'y
Cuts at: 1132 1229 2915 4460 1132
Size: 97 1686 1545 2229
Fragments arranged by size:
2229 1686 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5557
PciI A'CATG_T
Cuts at: 4456 4456
Size: 5557
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2086 1108
Size: 978 4579
PmeI GTTT'AAAC
Cuts at: 2861 2861
Size: 5557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 5139 5171 3626
Size: 32 1481 32 4012
Fragments arranged by size:
4012 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5557
PsiI TTA'TAA
Cuts at: 3127 3127
Size: 5557
PstI C_TGCA'G
Cuts at: 830 2034 2909 830
Size: 1204 875 3478
Fragments arranged by size:
3478 1204 875
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5557
PvuII CAG'CTG
Cuts at: 2489 2489
Size: 5557
SalI G'TCGA_C
Cuts at: 1936 2899 1936
Size: 963 4594
SbfI CC_TGCA'GG
Cuts at: 2909 2909
Size: 5557
ScaI AGT'ACT
Cuts at: 3805 5517 3805
Size: 1712 3845
SfcI C'TryA_G
Cuts at: 826 1045 2030 2905 4040 4721 4912 826
Size: 219 985 875 1135 681 191 1471
Fragments arranged by size:
1471 1135 985 875 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5557
SmaI CCC'GGG
Cuts at: 1872 2884 1872
Size: 1012 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3661 4562 4824 820
Size: 197 926 1718 901 262 1553
Fragments arranged by size:
1718 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3348 493
Size: 2855 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5557
SphI G_CATG'C
Cuts at: 1132 2915 1132
Size: 1783 3774
SspI AAT'ATT
Cuts at: 5 52 3481 5
Size: 47 3429 2081
Fragments arranged by size:
3429 2081 47
StuI AGG'CCT
Cuts at: 1517 2766 1517
Size: 1249 4308
StyI C'CwwG_G
Cuts at: 513 1065 2004 2996 513
Size: 552 939 992 3074
Fragments arranged by size:
3074 992 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3584 3601 3754 3939 4384 325
Size: 1255 2004 17 153 185 445 1498
Fragments arranged by size:
2004 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3803 5515 96
Size: 274 80 33 51 1443 1826 1712 138
Fragments arranged by size:
1826 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5517 5549 5517
Size: 32 5525
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5557
XbaI T'CTAG_A
Cuts at: 2893 2893
Size: 5557
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5557
XmaI C'CCGG_G
Cuts at: 1870 2882 1870
Size: 1012 4545
XmnI GAAnn'nnTTC
Cuts at: 3686 3686
Size: 5557
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3365 276
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI