Restriction map for
pFN21AE3403
(Circular) (Six-base) MAPSORT of: pFN21AE3403.seq Check: 2284 from: 1 to: 5215
pFN21AE3403 5215 bp
With 182 enzymes: SgfI *
March 28, 2012 12:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3025 278
Size: 53 83 186 936 1489 2468
Fragments arranged by size:
2468 1489 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2558 1937
Size: 621 4594
Acc65I G'GTAC_C
Cuts at: 2536 2536
Size: 5215
AclI AA'CG_TT
Cuts at: 1563 3342 3715 1563
Size: 1779 373 3063
Fragments arranged by size:
3063 1779 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 3278 4662 4930 1632
Size: 422 1224 1384 268 1917
Fragments arranged by size:
1917 1384 1224 422 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5018
AflIII A'CryG_T
Cuts at: 1203 4114 5091 1203
Size: 2911 977 1327
Fragments arranged by size:
2911 1327 977
AgeI A'CCGG_T
Cuts at: 4046 4046
Size: 5215
AhdI GACnn_n'nnGTC
Cuts at: 3944 3944
Size: 5215
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5215
AlwNI CAG_nnn'CTG
Cuts at: 2958 4530 2958
Size: 1572 3643
ApaLI G'TGCA_C
Cuts at: 3272 4428 3272
Size: 1156 4059
ApoI r'AATT_y
Cuts at: 1493 2081 2410 2524 2750 2902 5011 1493
Size: 588 329 114 226 152 2109 1697
Fragments arranged by size:
2109 1697 588 329 226 152 114
AseI AT'TA_AT
Cuts at: 160 1032 2337 3769 160
Size: 872 1305 1432 1606
Fragments arranged by size:
1606 1432 1305 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5215
AvaI C'yCGr_G
Cuts at: 1870 1943 2540 1870
Size: 73 597 4545
Fragments arranged by size:
4545 597 73
BamHI G'GATC_C
Cuts at: 2545 2912 2545
Size: 367 4848
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2448 2536 3991 618
Size: 325 217 627 661 88 1455 1842
Fragments arranged by size:
1842 1455 661 627 325 217 88
BanII G_rGCy'C
Cuts at: 1391 2534 1391
Size: 1143 4072
BbeI G_GCGC'C
Cuts at: 2452 2452
Size: 5215
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2071 2202 928
Size: 971 172 131 3941
Fragments arranged by size:
3941 971 172 131
BbvCI CC'TCA_GC
Cuts at: 2484 2484
Size: 5215
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2426 2460 3406 3440 1903
Size: 34 50 34 405 34 946 34 3678
Fragments arranged by size:
3678 946 405 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3107 4317 3107
Size: 1210 4005
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5215
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3826 136
Size: 107 122 71 631 605 2154 1525
Fragments arranged by size:
2154 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5210 5210
Size: 5215
BlpI GC'TnA_GC
Cuts at: 2632 2632
Size: 5215
Bme1580I G_kGCm'C
Cuts at: 1790 3276 4432 1790
Size: 1486 1156 2573
Fragments arranged by size:
2573 1486 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5215
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2946 3904 442
Size: 416 53 83 399 24 1529 958 1753
Fragments arranged by size:
1753 1529 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2399 1056
Size: 1343 3872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3875 1239
Size: 2636 2579
Bpu10I CC'TnA_GC
Cuts at: 2484 2484
Size: 5215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3340 4205 4503 3340
Size: 865 298 4052
Fragments arranged by size:
4052 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3878 882
Size: 617 2379 2219
Fragments arranged by size:
2379 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3006 493
Size: 711 1802 2702
Fragments arranged by size:
2702 1802 711
BsaBI GATnn'nnATC
Cuts at: 5209 5209
Size: 5215
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2449 3022 3404 4039
Size: 53 83 186 936 916 573 382 635
Cuts at: 4039 275
Size: 1451
Fragments arranged by size:
1451 936 916 635 573 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3648 4046 4320 4467 4878 3648
Size: 398 274 147 411 3985
Fragments arranged by size:
3985 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2088 2118 1178
Size: 30 165 30 685 30 4275
Fragments arranged by size:
4275 685 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5215
BseYI C'CCAG_C
Cuts at: 2175 4418 4914 2175
Size: 2243 496 2476
Fragments arranged by size:
2476 2243 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4642
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2665 3426 4454 1540
Size: 275 186 664 761 1028 2301
Fragments arranged by size:
2301 1028 761 664 275 186
BsiHKAI G_wGCw'C
Cuts at: 3276 3361 4432 3276
Size: 85 1071 4059
Fragments arranged by size:
4059 1071 85
BsmI GAATG_Cn'
Cuts at: 2726 2819 2726
Size: 93 5122
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2534 3276 3361 4432 1391
Size: 399 744 742 85 1071 2174
Fragments arranged by size:
2174 1071 744 742 399 85
BspEI T'CCGG_A
Cuts at: 4878 4878
Size: 5215
BspHI T'CATG_A
Cuts at: 3104 4834 3104
Size: 1730 3485
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2570 844
Size: 1726 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3102 1394
Size: 429 1279 3507
Fragments arranged by size:
3507 1279 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2518 3710 3884 65
Size: 1183 1270 1192 174 1396
Fragments arranged by size:
1396 1270 1192 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3859 4046 1700
Size: 110 2049 187 2869
Fragments arranged by size:
2869 2049 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5215
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5215
BssSI C'ACGA_G
Cuts at: 3275 4287 3275
Size: 1012 4203
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5215
BstBI TT'CG_AA
Cuts at: 4019 4019
Size: 5215
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5215
BstYI r'GATC_y
Cuts at: 1972 2545 2912 3297 3314 4766 4852 4864 5210
Size: 573 367 385 17 1452 86 12 346
Cuts at: 5210 1972
Size: 1977
Fragments arranged by size:
1977 1452 573 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4663
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4476
Fragments arranged by size:
4476 683 56
BtsI GCAGTG_nn'
Cuts at: 2086 2731 3525 3545 2086
Size: 645 794 20 3756
Fragments arranged by size:
3756 794 645 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5215
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5180
DraI TTT'AAA
Cuts at: 2519 2519
Size: 5215
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4222 809
Size: 507 2906 1802
Fragments arranged by size:
2906 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2662 3551 5180
Size: 54 1425 50 275 18 832 889 1629
Cuts at: 5180 8
Size: 43
Fragments arranged by size:
1629 1425 889 832 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2662 1537
Size: 275 850 4090
Fragments arranged by size:
4090 850 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3145 1353
Size: 1792 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3802 4176 4322 387
Size: 1064 138 2213 374 146 1280
Fragments arranged by size:
2213 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 3278 3875 4662 4930 1239
Size: 393 422 1224 597 787 268 1524
Fragments arranged by size:
1524 1224 787 597 422 393 268
EcoRI G'AATT_C
Cuts at: 2410 2524 2902 2410
Size: 114 378 4723
Fragments arranged by size:
4723 378 114
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2084 2084
Size: 5215
FspI TGC'GCA
Cuts at: 3721 3721
Size: 5215
HaeII r_GCGC'y
Cuts at: 1678 2452 4362 1678
Size: 774 1910 2531
Fragments arranged by size:
2531 1910 774
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2088 2120 3856 3888 3930
Size: 32 135 32 516 32 1736 32 42
Cuts at: 3930 3962 1373
Size: 32 2626
Fragments arranged by size:
2626 1736 516 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2559 2805 669
Size: 988 281 621 246 3079
Fragments arranged by size:
3079 988 621 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5215
HpaI GTT'AAC
Cuts at: 2805 2805
Size: 5215
KasI G'GCGC_C
Cuts at: 2448 2448
Size: 5215
KpnI G_GTAC'C
Cuts at: 2540 2540
Size: 5215
MfeI C'AATT_G
Cuts at: 2814 2814
Size: 5215
MluI A'CGCG_T
Cuts at: 5091 5091
Size: 5215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2106 2143 4329 4513 1305
Size: 801 37 2186 184 2007
Fragments arranged by size:
2186 2007 801 184 37
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3393
Fragments arranged by size:
3393 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 3173 3532 3691 518
Size: 648 1224 783 359 159 2042
Fragments arranged by size:
2042 1224 783 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2631 3308 4456 4701 1606
Size: 1025 677 1148 245 2120
Fragments arranged by size:
2120 1148 1025 677 245
NarI GG'CG_CC
Cuts at: 2449 2449
Size: 5215
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4663
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5215
NheI G'CTAG_C
Cuts at: 1052 2395 1052
Size: 1343 3872
NotI GC'GGCC_GC
Cuts at: 2662 2662
Size: 5215
NspI r_CATG'y
Cuts at: 1132 1229 2573 4118 1132
Size: 97 1344 1545 2229
Fragments arranged by size:
2229 1545 1344 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5215
PciI A'CATG_T
Cuts at: 4114 4114
Size: 5215
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5215
PmeI GTTT'AAAC
Cuts at: 2519 2519
Size: 5215
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3284 3316 4797 4829 3284
Size: 32 1481 32 3670
Fragments arranged by size:
3670 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5215
PsiI TTA'TAA
Cuts at: 2785 2785
Size: 5215
PstI C_TGCA'G
Cuts at: 830 2567 830
Size: 1737 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5215
SalI G'TCGA_C
Cuts at: 1936 2557 1936
Size: 621 4594
SbfI CC_TGCA'GG
Cuts at: 2567 2567
Size: 5215
ScaI AGT'ACT
Cuts at: 3463 5175 3463
Size: 1712 3503
SfcI C'TryA_G
Cuts at: 826 1045 2563 3698 4379 4570 826
Size: 219 1518 1135 681 191 1471
Fragments arranged by size:
1518 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2450 2450
Size: 5215
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5215
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5215
SmaI CCC'GGG
Cuts at: 1872 2542 1872
Size: 670 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3319 4220 4482 820
Size: 197 926 1376 901 262 1553
Fragments arranged by size:
1553 1376 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3006 493
Size: 2513 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5215
SphI G_CATG'C
Cuts at: 1132 2573 1132
Size: 1441 3774
SspI AAT'ATT
Cuts at: 5 52 3139 5
Size: 47 3087 2081
Fragments arranged by size:
3087 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5215
StyI C'CwwG_G
Cuts at: 513 1065 2654 513
Size: 552 1589 3074
Fragments arranged by size:
3074 1589 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3242 3259 3412 3597 4042 325
Size: 1255 1662 17 153 185 445 1498
Fragments arranged by size:
1662 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3461 5173 96
Size: 274 80 33 51 1443 1484 1712 138
Fragments arranged by size:
1712 1484 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5175 5207 5175
Size: 32 5183
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5215
XbaI T'CTAG_A
Cuts at: 2182 2551 2182
Size: 369 4846
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5215
XmaI C'CCGG_G
Cuts at: 1870 2540 1870
Size: 670 4545
XmnI GAAnn'nnTTC
Cuts at: 3344 3344
Size: 5215
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3023 276
Size: 53 83 186 936 1489 2468
Fragments arranged by size:
2468 1489 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI