Restriction map for
pFN21AE3390
(Circular) (Six-base) MAPSORT of: pFN21AE3390.seq Check: 7575 from: 1 to: 8317
pFN21AE3390 8317 bp
With 182 enzymes: SgfI *
January 18, 2013 17:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2330 2330
Size: 8317
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4489 6127 278
Size: 53 83 186 936 2953 1638 2468
Fragments arranged by size:
2953 2468 1638 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5660 1937
Size: 3723 4594
Acc65I G'GTAC_C
Cuts at: 5638 5638
Size: 8317
AclI AA'CG_TT
Cuts at: 1563 6444 6817 1563
Size: 4881 373 3063
Fragments arranged by size:
4881 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2232 2492 2622 2952 3069 3141 3489 3936
Size: 600 260 130 330 117 72 348 447
Cuts at: 3936 3969 4107 4485 4499 4790 5297 5423 6380
Size: 33 138 378 14 291 507 126 957
Cuts at: 6380 7764 8032 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 957 600 507 447 378 348
330 291 268 260 138 130 126 117
72 33 14
AflII C'TTAA_G
Cuts at: 820 1017 5027 820
Size: 197 4010 4110
Fragments arranged by size:
4110 4010 197
AflIII A'CryG_T
Cuts at: 1203 2071 3656 7216 8193 1203
Size: 868 1585 3560 977 1327
Fragments arranged by size:
3560 1585 1327 977 868
AgeI A'CCGG_T
Cuts at: 7148 7148
Size: 8317
AhdI GACnn_n'nnGTC
Cuts at: 7046 7046
Size: 8317
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8317
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3993 4025 4566 4598 3993
Size: 32 541 32 7712
Fragments arranged by size:
7712 541 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2863 4223 4259 5401 6060 7632 2863
Size: 1360 36 1142 659 1572 3548
Fragments arranged by size:
3548 1572 1360 1142 659 36
ApaLI G'TGCA_C
Cuts at: 6374 7530 6374
Size: 1156 7161
ApoI r'AATT_y
Cuts at: 1493 2330 2794 2989 3141 4059 4294 4394 4578
Size: 837 464 195 152 918 235 100 184
Cuts at: 4578 4754 5579 5626 5852 6004 8113 1493
Size: 176 825 47 226 152 2109 1697
Fragments arranged by size:
2109 1697 918 837 825 464 235 226
195 184 176 152 152 100 47
AseI AT'TA_AT
Cuts at: 160 1032 3195 3336 3755 3759 4143 6871 160
Size: 872 2163 141 419 4 384 2728 1606
Fragments arranged by size:
2728 2163 1606 872 419 384 141 4
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8317
AvaI C'yCGr_G
Cuts at: 1870 1943 4920 5642 1870
Size: 73 2977 722 4545
Fragments arranged by size:
4545 2977 722 73
AvrII C'CTAG_G
Cuts at: 5462 5462
Size: 8317
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3019 3052 3019
Size: 33 8284
BamHI G'GATC_C
Cuts at: 2319 5647 6014 2319
Size: 3328 367 4622
Fragments arranged by size:
4622 3328 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5638 7093 618
Size: 325 217 627 3851 1455 1842
Fragments arranged by size:
3851 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4537 4921 4944 5636 1391
Size: 3146 384 23 692 4072
Fragments arranged by size:
4072 3146 692 384 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2103 2781 3321 3585 3609 3699 4369
Size: 971 204 678 540 264 24 90 670
Cuts at: 4369 928
Size: 4876
Fragments arranged by size:
4876 971 678 670 540 264 204 90
24
BbvCI CC'TCA_GC
Cuts at: 3702 4119 3702
Size: 417 7900
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4679 4713 6508 6542 1903
Size: 34 2742 34 1795 34 3678
Fragments arranged by size:
3678 2742 1795 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3700 6209 7419 3700
Size: 2509 1210 4598
Fragments arranged by size:
4598 2509 1210
BclI T'GATC_A
Cuts at: 1548 2263 3627 1548
Size: 715 1364 6238
Fragments arranged by size:
6238 1364 715
BfrBI ATG'CAT
Cuts at: 2429 2429
Size: 8317
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6928 136
Size: 107 122 71 631 605 5256 1525
Fragments arranged by size:
5256 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2028 2467 5308 8312 2028
Size: 439 2841 3004 2033
Fragments arranged by size:
3004 2841 2033 439
BlpI GC'TnA_GC
Cuts at: 5277 5734 5277
Size: 457 7860
Bme1580I G_kGCm'C
Cuts at: 1790 6378 7534 1790
Size: 4588 1156 2573
Fragments arranged by size:
4588 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8317
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2132 4131 5541
Size: 416 53 83 399 24 715 1999 1410
Cuts at: 5541 6048 7006 442
Size: 507 958 1753
Fragments arranged by size:
1999 1753 1410 958 715 507 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8317
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3109 5292 3109
Size: 2183 6134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3543 6977 1239
Size: 2304 3434 2579
Fragments arranged by size:
3434 2579 2304
Bpu10I CC'TnA_GC
Cuts at: 3702 4119 3702
Size: 417 7900
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2393 3498 5091 6442 7307 7605 2393
Size: 1105 1593 1351 865 298 3105
Fragments arranged by size:
3105 1593 1351 1105 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2162 2371 5452 6980 882
Size: 617 663 209 3081 1528 2219
Fragments arranged by size:
3081 2219 1528 663 617 209
BsaAI yAC'GTr
Cuts at: 493 1204 2074 6108 493
Size: 711 870 4034 2702
Fragments arranged by size:
4034 2702 870 711
BsaBI GATnn'nnATC
Cuts at: 4342 8311 4342
Size: 3969 4348
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4486 6124 6506 7141
Size: 53 83 186 936 2953 1638 382 635
Cuts at: 7141 275
Size: 1451
Fragments arranged by size:
2953 1638 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5609 6750 7148 7422 7569 7980 5609
Size: 1141 398 274 147 411 5946
Fragments arranged by size:
5946 1141 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8092
Fragments arranged by size:
8092 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2990 1186
Size: 1804 6513
BseYI C'CCAG_C
Cuts at: 5594 7520 8016 5594
Size: 1926 496 5895
Fragments arranged by size:
5895 1926 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2358 5223 1163
Size: 573 622 2865 4257
Fragments arranged by size:
4257 2865 622 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5767 6528 7556 1540
Size: 275 186 3766 761 1028 2301
Fragments arranged by size:
3766 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4692 4921 4944 6378 6463 7534 4692
Size: 229 23 1434 85 1071 5475
Fragments arranged by size:
5475 1434 1071 229 85 23
BsmI GAATG_Cn'
Cuts at: 5549 5602 5828 5921 5549
Size: 53 226 93 7945
Fragments arranged by size:
7945 226 93 53
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4537 4692 4921 4944 5636 6378 6463
Size: 399 2747 155 229 23 692 742 85
Cuts at: 6463 7534 1391
Size: 1071 2174
Fragments arranged by size:
2747 2174 1071 742 692 399 229 155
85 23
BspEI T'CCGG_A
Cuts at: 7980 7980
Size: 8317
BspHI T'CATG_A
Cuts at: 2710 4911 6206 7936 2710
Size: 2201 1295 1730 3091
Fragments arranged by size:
3091 2201 1730 1295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2330 3013 5672 844
Size: 1486 683 2659 3489
Fragments arranged by size:
3489 2659 1486 683
BsrBI CCG'CTC
Cuts at: 1394 1823 6204 1394
Size: 429 4381 3507
Fragments arranged by size:
4381 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3234 6812 6986 65
Size: 1183 1986 3578 174 1396
Fragments arranged by size:
3578 1986 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6961 7148 1700
Size: 110 5151 187 2869
Fragments arranged by size:
5151 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2148 96
Size: 2052 6265
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8317
BssSI C'ACGA_G
Cuts at: 2545 5036 6377 7389 2545
Size: 2491 1341 1012 3473
Fragments arranged by size:
3473 2491 1341 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2354 5353 794
Size: 1560 2999 3758
Fragments arranged by size:
3758 2999 1560
BstBI TT'CG_AA
Cuts at: 7121 7121
Size: 8317
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8317
BstXI CCAn_nnnn'nTGG
Cuts at: 2784 2784
Size: 8317
BstYI r'GATC_y
Cuts at: 1972 2028 2319 2467 5308 5647 6014 6399 6416
Size: 56 291 148 2841 339 367 385 17
Cuts at: 6416 7868 7954 7966 8312 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2841 1977 1452 385 367 346 339 291
148 86 56 17 12
Bsu36I CC'TnA_GG
Cuts at: 3780 3780
Size: 8317
BtgI C'CryG_G
Cuts at: 513 1065 3768 513
Size: 552 2703 5062
Fragments arranged by size:
5062 2703 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7578
Fragments arranged by size:
7578 683 56
BtsI GCAGTG_nn'
Cuts at: 4266 5146 5327 5833 6627 6647 4266
Size: 880 181 506 794 20 5936
Fragments arranged by size:
5936 880 794 506 181 20
ClaI AT'CG_AT
Cuts at: 1554 4340 1554
Size: 2786 5531
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8282
DraI TTT'AAA
Cuts at: 5524 5621 5524
Size: 97 8220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5536 7324 809
Size: 507 4220 1788 1802
Fragments arranged by size:
4220 1802 1788 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5160 5764 6653
Size: 54 1425 50 275 18 3330 604 889
Cuts at: 6653 8282 8
Size: 1629 43
Fragments arranged by size:
3330 1629 1425 889 604 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5764 1537
Size: 275 3952 4090
Fragments arranged by size:
4090 3952 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 2557 2967 3275 4518 5168 5478 6247
Size: 719 485 410 308 1243 650 310 769
Cuts at: 6247 1353
Size: 3423
Fragments arranged by size:
3423 1243 769 719 650 485 410 310
308
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2534 6904 7278 7424 387
Size: 1064 138 945 4370 374 146 1280
Fragments arranged by size:
4370 1280 1064 945 374 146 138
EcoICRI GAG'CTC
Cuts at: 4919 4942 4919
Size: 23 8294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2492 2622 2952 3069 3141 3489
Size: 393 600 260 130 330 117 72 348
Cuts at: 3489 3543 3936 3969 4107 4485 4499 4790 5297
Size: 54 393 33 138 378 14 291 507
Cuts at: 5297 5423 6380 6977 7764 8032 1239
Size: 126 957 597 787 268 1524
Fragments arranged by size:
1524 957 787 600 597 507 393 393
378 348 330 291 268 260 138 130
126 117 72 54 33 14
EcoO109I rG'GnC_Cy
Cuts at: 2083 3776 2083
Size: 1693 6624
EcoRI G'AATT_C
Cuts at: 5626 6004 5626
Size: 378 7939
EcoRV GAT'ATC
Cuts at: 2509 4344 2509
Size: 1835 6482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2237 2836 3077 3584 2237
Size: 599 241 507 6970
Fragments arranged by size:
6970 599 507 241
FspI TGC'GCA
Cuts at: 6823 6823
Size: 8317
HaeII r_GCGC'y
Cuts at: 1678 4591 5035 7464 1678
Size: 2913 444 2429 2531
Fragments arranged by size:
2913 2531 2429 444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2645 2677 2839
Size: 32 135 32 620 32 421 32 162
Cuts at: 2839 2871 4322 4354 6958 6990 7032 7064 1373
Size: 32 1451 32 2604 32 42 32 2626
Fragments arranged by size:
2626 2604 1451 620 421 162 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5661 5907 669
Size: 988 281 3723 246 3079
Fragments arranged by size:
3723 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2282 3290 3455 748
Size: 1534 1008 165 5610
Fragments arranged by size:
5610 1534 1008 165
HpaI GTT'AAC
Cuts at: 5907 5907
Size: 8317
KpnI G_GTAC'C
Cuts at: 5642 5642
Size: 8317
MfeI C'AATT_G
Cuts at: 5916 5916
Size: 8317
MluI A'CGCG_T
Cuts at: 8193 8193
Size: 8317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2916 7431 7615 1305
Size: 1611 4515 184 2007
Fragments arranged by size:
4515 2007 1611 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5162 10
Size: 54 1425 343 3330 3165
Fragments arranged by size:
3330 3165 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2782 6275 6634 6793 518
Size: 648 1616 3493 359 159 2042
Fragments arranged by size:
3493 2042 1616 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2860 4975 5353 5733 6410 7558 7803 1606
Size: 1254 2115 378 380 677 1148 245 2120
Fragments arranged by size:
2120 2115 1254 1148 677 380 378 245
NcoI C'CATG_G
Cuts at: 513 1065 3768 513
Size: 552 2703 5062
Fragments arranged by size:
5062 2703 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8317
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8317
NotI GC'GGCC_GC
Cuts at: 5764 5764
Size: 8317
NsiI A_TGCA'T
Cuts at: 2431 2431
Size: 8317
NspI r_CATG'y
Cuts at: 1132 1229 3660 5675 7220 1132
Size: 97 2431 2015 1545 2229
Fragments arranged by size:
2431 2229 2015 1545 97
PacI TTA_AT'TAA
Cuts at: 3759 3759
Size: 8317
PasI CC'CwG_GG
Cuts at: 1348 2665 1348
Size: 1317 7000
PciI A'CATG_T
Cuts at: 3656 7216 3656
Size: 3560 4757
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5377 1108
Size: 4269 4048
PmeI GTTT'AAAC
Cuts at: 5621 5621
Size: 8317
PmlI CAC'GTG
Cuts at: 2074 2074
Size: 8317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6386 6418 7899 7931 6386
Size: 32 1481 32 6772
Fragments arranged by size:
6772 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 3776 2083
Size: 1693 6624
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8317
PsiI TTA'TAA
Cuts at: 3461 5887 3461
Size: 2426 5891
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4941 4973 4941
Size: 32 8285
PstI C_TGCA'G
Cuts at: 830 2868 5358 5669 830
Size: 2038 2490 311 3478
Fragments arranged by size:
3478 2490 2038 311
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8317
PvuII CAG'CTG
Cuts at: 2860 4975 5353 2860
Size: 2115 378 5824
Fragments arranged by size:
5824 2115 378
SacI G_AGCT'C
Cuts at: 4921 4944 4921
Size: 23 8294
SalI G'TCGA_C
Cuts at: 1936 5659 1936
Size: 3723 4594
SapI GCTCTTCn'nnn_
Cuts at: 4518 4518
Size: 8317
SbfI CC_TGCA'GG
Cuts at: 5669 5669
Size: 8317
ScaI AGT'ACT
Cuts at: 6565 8277 6565
Size: 1712 6605
SfcI C'TryA_G
Cuts at: 826 1045 2864 4095 4559 5354 5501 5665 6800
Size: 219 1819 1231 464 795 147 164 1135
Cuts at: 6800 7481 7672 826
Size: 681 191 1471
Fragments arranged by size:
1819 1471 1231 1135 795 681 464 219
191 164 147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8317
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8317
SmaI CCC'GGG
Cuts at: 1872 5644 1872
Size: 3772 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2372 3513 4920 5027 5106 6421
Size: 197 926 429 1141 1407 107 79 1315
Cuts at: 6421 7322 7584 820
Size: 901 262 1553
Fragments arranged by size:
1553 1407 1315 1141 926 901 429 262
197 107 79
SnaBI TAC'GTA
Cuts at: 493 6108 493
Size: 5615 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8317
SphI G_CATG'C
Cuts at: 1132 5675 1132
Size: 4543 3774
SspI AAT'ATT
Cuts at: 5 52 3391 3541 4876 6241 5
Size: 47 3339 150 1335 1365 2081
Fragments arranged by size:
3339 2081 1365 1335 150 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8317
StyI C'CwwG_G
Cuts at: 513 1065 3768 5462 5756 513
Size: 552 2703 1694 294 3074
Fragments arranged by size:
3074 2703 1694 552 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2283 3599 6344 6361 6514 6699 7144
Size: 1255 703 1316 2745 17 153 185 445
Cuts at: 7144 325
Size: 1498
Fragments arranged by size:
2745 1498 1316 1255 703 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 6563 8275
Size: 274 80 33 51 1443 171 4415 1712
Cuts at: 8275 96
Size: 138
Fragments arranged by size:
4415 1712 1443 274 171 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2828 2860 8277 8309 2828
Size: 32 5417 32 2836
Fragments arranged by size:
5417 2836 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8317
XbaI T'CTAG_A
Cuts at: 2287 4645 5653 2287
Size: 2358 1008 4951
Fragments arranged by size:
4951 2358 1008
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5155 5155
Size: 8317
XhoI C'TCGA_G
Cuts at: 1943 4920 1943
Size: 2977 5340
XmaI C'CCGG_G
Cuts at: 1870 5642 1870
Size: 3772 4545
XmnI GAAnn'nnTTC
Cuts at: 2566 4206 6446 2566
Size: 1640 2240 4437
Fragments arranged by size:
4437 2240 1640
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4487 6125 276
Size: 53 83 186 936 2953 1638 2468
Fragments arranged by size:
2953 2468 1638 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI BbeI BsiWI BsmBI BstZ17I DraIII
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
PfoI PspOMI PspXI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI