Restriction map for
pFN21AE3381
(Circular) (Six-base) MAPSORT of: pFN21AE3381.seq Check: 5733 from: 1 to: 8158
pFN21AE3381 8158 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5968 278
Size: 53 83 186 936 4432 2468
Fragments arranged by size:
4432 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2353 5501 1937
Size: 416 3148 4594
Fragments arranged by size:
4594 3148 416
Acc65I G'GTAC_C
Cuts at: 5479 5479
Size: 8158
AclI AA'CG_TT
Cuts at: 1563 6285 6658 1563
Size: 4722 373 3063
Fragments arranged by size:
4722 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3237 3528 3801 4075 4178 5105 6221 7605
Size: 1605 291 273 274 103 927 1116 1384
Cuts at: 7605 7873 1632
Size: 268 1917
Fragments arranged by size:
1917 1605 1384 1116 927 291 274 273
268 103
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7961
AflIII A'CryG_T
Cuts at: 1203 5449 7057 8034 1203
Size: 4246 1608 977 1327
Fragments arranged by size:
4246 1608 1327 977
AgeI A'CCGG_T
Cuts at: 6989 6989
Size: 8158
AhdI GACnn_n'nnGTC
Cuts at: 2251 2460 6887 2251
Size: 209 4427 3522
Fragments arranged by size:
4427 3522 209
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8158
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8158
AlwNI CAG_nnn'CTG
Cuts at: 5901 7473 5901
Size: 1572 6586
ApaI G_GGCC'C
Cuts at: 3491 3491
Size: 8158
ApaLI G'TGCA_C
Cuts at: 2911 3698 4570 6215 7371 2911
Size: 787 872 1645 1156 3698
Fragments arranged by size:
3698 1645 1156 872 787
ApoI r'AATT_y
Cuts at: 1493 2150 2343 2956 2984 4627 4765 5283 5467
Size: 657 193 613 28 1643 138 518 184
Cuts at: 5467 5693 5845 7954 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1643 657 613 518 226 193
184 152 138 28
AseI AT'TA_AT
Cuts at: 160 1032 3993 6712 160
Size: 872 2961 2719 1606
Fragments arranged by size:
2961 2719 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8158
AvaI C'yCGr_G
Cuts at: 1870 1943 2934 5483 1870
Size: 73 991 2549 4545
Fragments arranged by size:
4545 2549 991 73
BamHI G'GATC_C
Cuts at: 5488 5855 5488
Size: 367 7791
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5479 6934 618
Size: 325 217 627 3692 1455 1842
Fragments arranged by size:
3692 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2941 3156 3305 3426 3491 5477 1391
Size: 1550 215 149 121 65 1986 4072
Fragments arranged by size:
4072 1986 1550 215 149 121 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2432 2508 2874 3225 5220 928
Size: 971 533 76 366 351 1995 3866
Fragments arranged by size:
3866 1995 971 533 366 351 76
BbvCI CC'TCA_GC
Cuts at: 2697 5205 5290 2697
Size: 2508 85 5565
Fragments arranged by size:
5565 2508 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6349 6383 1903
Size: 34 4412 34 3678
Fragments arranged by size:
4412 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6050 7260 6050
Size: 1210 6948
BclI T'GATC_A
Cuts at: 1548 3045 1548
Size: 1497 6661
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3114 6769 136
Size: 107 122 71 631 605 1442 3655 1525
Fragments arranged by size:
3655 1525 1442 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3549 4495 8153 3549
Size: 946 3658 3554
Fragments arranged by size:
3658 3554 946
BlpI GC'TnA_GC
Cuts at: 4851 5575 4851
Size: 724 7434
Bme1580I G_kGCm'C
Cuts at: 1790 2878 2900 2915 3491 3702 4491 4574 6219
Size: 1088 22 15 576 211 789 83 1645
Cuts at: 6219 7375 1790
Size: 1156 2573
Fragments arranged by size:
2573 1645 1156 1088 789 576 211 83
22 15
BmgBI CAC'GTC
Cuts at: 1318 4486 4539 1318
Size: 3168 53 4937
Fragments arranged by size:
4937 3168 53
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2302 4017 5889
Size: 416 53 83 399 24 885 1715 1872
Cuts at: 5889 6847 442
Size: 958 1753
Fragments arranged by size:
1872 1753 1715 958 885 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8158
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5290 5290
Size: 8158
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2295 2333 3435 3955 4932 6818 1239
Size: 1056 38 1102 520 977 1886 2579
Fragments arranged by size:
2579 1886 1102 1056 977 520 38
Bpu10I CC'TnA_GC
Cuts at: 2436 2697 4245 5205 5290 2436
Size: 261 1548 960 85 5304
Fragments arranged by size:
5304 1548 960 261 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2599 3066 3522 4308 6283 7148 7446 2599
Size: 467 456 786 1975 865 298 3311
Fragments arranged by size:
3311 1975 865 786 467 456 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3355 5155 6821 882
Size: 617 1856 1800 1666 2219
Fragments arranged by size:
2219 1856 1800 1666 617
BsaAI yAC'GTr
Cuts at: 493 1204 5949 493
Size: 711 4745 2702
Fragments arranged by size:
4745 2702 711
BsaBI GATnn'nnATC
Cuts at: 3690 8152 3690
Size: 4462 3696
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5965 6347 6982 275
Size: 53 83 186 936 4432 382 635 1451
Fragments arranged by size:
4432 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2848 6591 6989 7263 7410 7821 2848
Size: 3743 398 274 147 411 3185
Fragments arranged by size:
3743 3185 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2093 2123 3053 3083 4525
Size: 30 165 30 690 30 930 30 1442
Cuts at: 4525 4555 1178
Size: 30 4781
Fragments arranged by size:
4781 1442 930 690 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 8158
BseYI C'CCAG_C
Cuts at: 3829 7361 7857 3829
Size: 3532 496 4130
Fragments arranged by size:
4130 3532 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2649 4629 1163
Size: 573 913 1980 4692
Fragments arranged by size:
4692 1980 913 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2048 5608 6369 7397 1540
Size: 275 186 47 3560 761 1028 2301
Fragments arranged by size:
3560 2301 1028 761 275 186 47
BsiHKAI G_wGCw'C
Cuts at: 2915 2941 3156 3305 3702 4574 6219 6304 7375
Size: 26 215 149 397 872 1645 85 1071
Cuts at: 7375 2915
Size: 3698
Fragments arranged by size:
3698 1645 1071 872 397 215 149 85
26
BsmI GAATG_Cn'
Cuts at: 5669 5762 5669
Size: 93 8065
BsmBI CGTCTCn'nnnn_
Cuts at: 4545 4545
Size: 8158
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2878 2900 2915 2941 3156 3305 3426
Size: 399 1088 22 15 26 215 149 121
Cuts at: 3426 3491 3702 4491 4574 5477 6219 6304 7375
Size: 65 211 789 83 903 742 85 1071
Cuts at: 7375 1391
Size: 2174
Fragments arranged by size:
2174 1088 1071 903 789 742 399 215
211 149 121 85 83 65 26 22
15
BspEI T'CCGG_A
Cuts at: 7821 7821
Size: 8158
BspHI T'CATG_A
Cuts at: 6047 7777 6047
Size: 1730 6428
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5173 5513 844
Size: 4329 340 3489
Fragments arranged by size:
4329 3489 340
BsrBI CCG'CTC
Cuts at: 1394 1823 2791 4528 6045 1394
Size: 429 968 1737 1517 3507
Fragments arranged by size:
3507 1737 1517 968 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4857 6653 6827 65
Size: 1183 3609 1796 174 1396
Fragments arranged by size:
3609 1796 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4095 5367 6802 6989 1700
Size: 110 2285 1272 1435 187 2869
Fragments arranged by size:
2869 2285 1435 1272 187 110
BsrGI T'GTAC_A
Cuts at: 96 2526 2552 96
Size: 2430 26 5702
Fragments arranged by size:
5702 2430 26
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8158
BssSI C'ACGA_G
Cuts at: 4565 5233 6218 7230 4565
Size: 668 985 1012 5493
Fragments arranged by size:
5493 1012 985 668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4578 794
Size: 3784 4374
BstBI TT'CG_AA
Cuts at: 6962 6962
Size: 8158
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8158
BstYI r'GATC_y
Cuts at: 1972 3549 4495 5238 5488 5855 6240 6257 7709
Size: 1577 946 743 250 367 385 17 1452
Cuts at: 7709 7795 7807 8153 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1577 1452 946 743 385 367 346
250 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2709 3528 513
Size: 552 1644 819 5143
Fragments arranged by size:
5143 1644 819 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7419
Fragments arranged by size:
7419 683 56
BtsI GCAGTG_nn'
Cuts at: 2830 3030 3103 5674 6468 6488 2830
Size: 200 73 2571 794 20 4500
Fragments arranged by size:
4500 2571 794 200 73 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8158
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2134 2169 2716 2749 2751 2784 529
Size: 35 1570 35 547 33 2 33 5903
Fragments arranged by size:
5903 1570 547 35 35 33 33 2
DraI TTT'AAA
Cuts at: 5462 5462
Size: 8158
DraIII CAC_nnn'GTG
Cuts at: 2966 4578 2966
Size: 1612 6546
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4537 7165 809
Size: 507 3221 2628 1802
Fragments arranged by size:
3221 2628 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2045 3802 5605
Size: 54 1425 50 275 18 215 1757 1803
Cuts at: 5605 6494 8123 8
Size: 889 1629 43
Fragments arranged by size:
1803 1757 1629 1425 889 275 215 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2045 5605 1537
Size: 275 233 3560 4090
Fragments arranged by size:
4090 3560 275 233
EarI CTCTTCn'nnn_
Cuts at: 1353 2577 4154 6088 1353
Size: 1224 1577 1934 3423
Fragments arranged by size:
3423 1934 1577 1224
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6745 7119 7265 387
Size: 1064 138 5156 374 146 1280
Fragments arranged by size:
5156 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2939 3154 3303 2939
Size: 215 149 7794
Fragments arranged by size:
7794 215 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2295 2333 3237 3435 3528 3801 3955
Size: 393 663 38 904 198 93 273 154
Cuts at: 3955 4075 4178 4932 5105 6221 6818 7605 7873
Size: 120 103 754 173 1116 597 787 268
Cuts at: 7873 1239
Size: 1524
Fragments arranged by size:
1524 1116 904 787 754 663 597 393
273 268 198 173 154 120 103 93
38
EcoO109I rG'GnC_Cy
Cuts at: 2440 3488 2440
Size: 1048 7110
EcoRI G'AATT_C
Cuts at: 2956 2984 5283 5467 5845 2956
Size: 28 2299 184 378 5269
Fragments arranged by size:
5269 2299 378 184 28
EcoRV GAT'ATC
Cuts at: 3523 4372 5380 3523
Size: 849 1008 6301
Fragments arranged by size:
6301 1008 849
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2422 3417 2422
Size: 995 7163
FspI TGC'GCA
Cuts at: 4205 6664 4205
Size: 2459 5699
FspAI rTGC'GCAy
Cuts at: 4205 4205
Size: 8158
HaeII r_GCGC'y
Cuts at: 1678 7305 1678
Size: 5627 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 2237 2269 2419
Size: 32 135 32 521 32 112 32 150
Cuts at: 2419 2446 2451 2478 2597 2629 3287 3319 4356
Size: 27 5 27 119 32 658 32 1037
Cuts at: 4356 4388 4525 4557 5210 5242 6799 6831 6873
Size: 32 137 32 653 32 1557 32 42
Cuts at: 6873 6905 1373
Size: 32 2626
Fragments arranged by size:
2626 1557 1037 658 653 521 150 137
135 119 112 42 32 32 32 32
32 32 32 32 32 32 32 27
27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2354 4670 5502 5748 669
Size: 988 281 416 2316 832 246 3079
Fragments arranged by size:
3079 2316 988 832 416 281 246
HindIII A'AGCT_T
Cuts at: 748 2137 3394 3540 3783 4074 748
Size: 1389 1257 146 243 291 4832
Fragments arranged by size:
4832 1389 1257 291 243 146
HpaI GTT'AAC
Cuts at: 5748 5748
Size: 8158
KpnI G_GTAC'C
Cuts at: 5483 5483
Size: 8158
MfeI C'AATT_G
Cuts at: 5757 5757
Size: 8158
MluI A'CGCG_T
Cuts at: 8034 8034
Size: 8158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4197 5372 7272 7456 1305
Size: 2892 1175 1900 184 2007
Fragments arranged by size:
2892 2007 1900 1175 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3804 10
Size: 54 1425 343 1972 4364
Fragments arranged by size:
4364 1972 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4461 6116 6475 6634 518
Size: 648 3295 1655 359 159 2042
Fragments arranged by size:
3295 2042 1655 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2689 3833 5574 6251 7399 7644 1606
Size: 1083 1144 1741 677 1148 245 2120
Fragments arranged by size:
2120 1741 1148 1144 1083 677 245
NaeI GCC'GGC
Cuts at: 4097 5369 4097
Size: 1272 6886
NcoI C'CATG_G
Cuts at: 513 1065 2709 3528 513
Size: 552 1644 819 5143
Fragments arranged by size:
5143 1644 819 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8158
NgoMIV G'CCGG_C
Cuts at: 4095 5367 4095
Size: 1272 6886
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8158
NotI GC'GGCC_GC
Cuts at: 5605 5605
Size: 8158
NspI r_CATG'y
Cuts at: 1132 1229 5441 5453 5516 7061 1132
Size: 97 4212 12 63 1545 2229
Fragments arranged by size:
4212 2229 1545 97 63 12
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8158
PciI A'CATG_T
Cuts at: 5449 7057 5449
Size: 1608 6550
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4958 5354 1108
Size: 3850 396 3912
Fragments arranged by size:
3912 3850 396
PfoI T'CCnGG_A
Cuts at: 2220 4378 2220
Size: 2158 6000
PmeI GTTT'AAAC
Cuts at: 5462 5462
Size: 8158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6227 6259 7740 7772 6227
Size: 32 1481 32 6613
Fragments arranged by size:
6613 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2440 2440
Size: 8158
PshAI GACnn'nnGTC
Cuts at: 1613 4665 1613
Size: 3052 5106
PsiI TTA'TAA
Cuts at: 5408 5728 5408
Size: 320 7838
PspOMI G'GGCC_C
Cuts at: 3487 3487
Size: 8158
PspXI vC'TCGA_Gb
Cuts at: 2934 2934
Size: 8158
PstI C_TGCA'G
Cuts at: 830 2802 2825 5510 830
Size: 1972 23 2685 3478
Fragments arranged by size:
3478 2685 1972 23
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8158
PvuII CAG'CTG
Cuts at: 2689 3833 2689
Size: 1144 7014
SacI G_AGCT'C
Cuts at: 2941 3156 3305 2941
Size: 215 149 7794
Fragments arranged by size:
7794 215 149
SalI G'TCGA_C
Cuts at: 1936 2352 5500 1936
Size: 416 3148 4594
Fragments arranged by size:
4594 3148 416
SapI GCTCTTCn'nnn_
Cuts at: 2577 2577
Size: 8158
SbfI CC_TGCA'GG
Cuts at: 5510 5510
Size: 8158
ScaI AGT'ACT
Cuts at: 6406 8118 6406
Size: 1712 6446
SfcI C'TryA_G
Cuts at: 826 1045 2798 2821 3022 5506 6641 7322 7513
Size: 219 1753 23 201 2484 1135 681 191
Cuts at: 7513 826
Size: 1471
Fragments arranged by size:
2484 1753 1471 1135 681 219 201 191
23
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8158
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8158
SmaI CCC'GGG
Cuts at: 1872 5485 1872
Size: 3613 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2614 2934 3081 3537 4323 6262
Size: 197 926 671 320 147 456 786 1939
Cuts at: 6262 7163 7425 820
Size: 901 262 1553
Fragments arranged by size:
1939 1553 926 901 786 671 456 320
262 197 147
SnaBI TAC'GTA
Cuts at: 493 5949 493
Size: 5456 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8158
SphI G_CATG'C
Cuts at: 1132 5516 1132
Size: 4384 3774
SspI AAT'ATT
Cuts at: 5 52 3466 5363 6082 5
Size: 47 3414 1897 719 2081
Fragments arranged by size:
3414 2081 1897 719 47
StuI AGG'CCT
Cuts at: 1517 4261 1517
Size: 2744 5414
StyI C'CwwG_G
Cuts at: 513 1065 2709 3528 3639 5597 513
Size: 552 1644 819 111 1958 3074
Fragments arranged by size:
3074 1958 1644 819 552 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4374 5061 6185 6202 6355 6540 6985
Size: 1255 2794 687 1124 17 153 185 445
Cuts at: 6985 325
Size: 1498
Fragments arranged by size:
2794 1498 1255 1124 687 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2526 2552 2814
Size: 274 80 33 51 1443 549 26 262
Cuts at: 2814 6404 8116 96
Size: 3590 1712 138
Fragments arranged by size:
3590 1712 1443 549 274 262 138 80
51 33 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3053 3085 8118 8150 3053
Size: 32 5033 32 3061
Fragments arranged by size:
5033 3061 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4537 1316
Size: 3221 4937
XbaI T'CTAG_A
Cuts at: 2941 5494 2941
Size: 2553 5605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2473 4552 2473
Size: 2079 6079
XhoI C'TCGA_G
Cuts at: 1943 2934 1943
Size: 991 7167
XmaI C'CCGG_G
Cuts at: 1870 5483 1870
Size: 3613 4545
XmnI GAAnn'nnTTC
Cuts at: 6287 6287
Size: 8158
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5966 276
Size: 53 83 186 936 4432 2468
Fragments arranged by size:
4432 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I Bsu36I EcoNI FseI KasI NarI
NruI NsiI PacI PmlI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI