Restriction map for
pFN21AE3371
(Circular) (Six-base) MAPSORT of: pFN21AE3371.seq Check: 9957 from: 1 to: 8011
pFN21AE3371 8011 bp
With 182 enzymes: SgfI *
February 3, 2015 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2490 2499 2615 4281
Size: 53 83 186 936 954 9 116 1666
Cuts at: 4281 5821 278
Size: 1540 2468
Fragments arranged by size:
2468 1666 1540 954 936 186 116 83
53 9
AccI GT'mk_AC
Cuts at: 1937 5354 1937
Size: 3417 4594
Acc65I G'GTAC_C
Cuts at: 5332 5332
Size: 8011
AclI AA'CG_TT
Cuts at: 1563 6138 6511 1563
Size: 4575 373 3063
Fragments arranged by size:
4575 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4070 4776 6074 7458 7726 1632
Size: 2438 706 1298 1384 268 1917
Fragments arranged by size:
2438 1917 1384 1298 706 268
AfeI AGC'GCT
Cuts at: 2238 4389 2238
Size: 2151 5860
AflII C'TTAA_G
Cuts at: 820 1017 3752 820
Size: 197 2735 5079
Fragments arranged by size:
5079 2735 197
AflIII A'CryG_T
Cuts at: 1203 3453 6910 7887 1203
Size: 2250 3457 977 1327
Fragments arranged by size:
3457 2250 1327 977
AgeI A'CCGG_T
Cuts at: 6842 6842
Size: 8011
AhdI GACnn_n'nnGTC
Cuts at: 6740 6740
Size: 8011
AleI CACnn'nnGTG
Cuts at: 1166 3045 1166
Size: 1879 6132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4202 1184
Size: 3018 4993
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 7979
AlwNI CAG_nnn'CTG
Cuts at: 2359 3895 3946 5754 7326 2359
Size: 1536 51 1808 1572 3044
Fragments arranged by size:
3044 1808 1572 1536 51
ApaLI G'TGCA_C
Cuts at: 2507 6068 7224 2507
Size: 3561 1156 3294
Fragments arranged by size:
3561 3294 1156
ApoI r'AATT_y
Cuts at: 1493 4007 4330 4674 5027 5320 5546 5698 7807
Size: 2514 323 344 353 293 226 152 2109
Cuts at: 7807 1493
Size: 1697
Fragments arranged by size:
2514 2109 1697 353 344 323 293 226
152
AseI AT'TA_AT
Cuts at: 160 1032 4938 6565 160
Size: 872 3906 1627 1606
Fragments arranged by size:
3906 1627 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8011
AvaI C'yCGr_G
Cuts at: 1870 1943 2398 5336 1870
Size: 73 455 2938 4545
Fragments arranged by size:
4545 2938 455 73
BamHI G'GATC_C
Cuts at: 5341 5708 5341
Size: 367 7644
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2061 2424 2729 5332 6787
Size: 325 217 627 274 363 305 2603 1455
Cuts at: 6787 618
Size: 1842
Fragments arranged by size:
2603 1842 1455 627 363 325 305 274
217
BanII G_rGCy'C
Cuts at: 1391 2115 2165 2399 3286 5330 1391
Size: 724 50 234 887 2044 4072
Fragments arranged by size:
4072 2044 887 724 234 50
BbeI G_GCGC'C
Cuts at: 2065 2733 2065
Size: 668 7343
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3486 5012 5148 928
Size: 971 1587 1526 136 3791
Fragments arranged by size:
3791 1587 1526 971 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2650 2684 3571 3605 6202 6236 1903
Size: 34 713 34 887 34 2597 34 3678
Fragments arranged by size:
3678 2597 887 713 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5903 7113 5903
Size: 1210 6801
BclI T'GATC_A
Cuts at: 1548 4863 5085 5212 1548
Size: 3315 222 127 4347
Fragments arranged by size:
4347 3315 222 127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6622 136
Size: 107 122 71 631 605 4950 1525
Fragments arranged by size:
4950 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2328 3300 8006 2328
Size: 972 4706 2333
Fragments arranged by size:
4706 2333 972
BlpI GC'TnA_GC
Cuts at: 5428 5428
Size: 8011
Bme1580I G_kGCm'C
Cuts at: 1790 2511 4905 6072 7228 1790
Size: 721 2394 1167 1156 2573
Fragments arranged by size:
2573 2394 1167 1156 721
BmgBI CAC'GTC
Cuts at: 1318 3513 1318
Size: 2195 5816
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3348 4111 5119
Size: 416 53 83 399 24 1931 763 1008
Cuts at: 5119 5742 6700 442
Size: 623 958 1753
Fragments arranged by size:
1931 1753 1008 958 763 623 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8011
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2261 2543 2919 3281 3303 3968 6671
Size: 794 228 282 376 362 22 665 2703
Cuts at: 6671 1239
Size: 2579
Fragments arranged by size:
2703 2579 794 665 376 362 282 228
22
Bpu10I CC'TnA_GC
Cuts at: 3594 3774 3594
Size: 180 7831
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2879 3266 4120 4463 6136 7001 7299 2879
Size: 387 854 343 1673 865 298 3591
Fragments arranged by size:
3591 1673 865 854 387 343 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2237 2713 6674 882
Size: 617 738 476 3961 2219
Fragments arranged by size:
3961 2219 738 617 476
BsaAI yAC'GTr
Cuts at: 493 1204 2185 2917 5802 493
Size: 711 981 732 2885 2702
Fragments arranged by size:
2885 2702 981 732 711
BsaBI GATnn'nnATC
Cuts at: 8005 8005
Size: 8011
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2062 2487 2496 2612
Size: 53 83 186 936 529 425 9 116
Cuts at: 2612 2730 4278 4656 5818 6200 6835 275
Size: 118 1548 378 1162 382 635 1451
Fragments arranged by size:
1548 1451 1162 936 635 529 425 382
378 186 118 116 83 53 9
BsaWI w'CCGG_w
Cuts at: 2391 6444 6842 7116 7263 7674 2391
Size: 4053 398 274 147 411 2728
Fragments arranged by size:
4053 2728 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2417 2447 2621 2651 3215
Size: 30 165 30 1014 30 174 30 564
Cuts at: 3215 3245 3279 3309 3429 3459 1178
Size: 30 34 30 120 30 5730
Fragments arranged by size:
5730 1014 564 174 165 120 34 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2453 3236 5201 1186
Size: 1267 783 1965 3996
Fragments arranged by size:
3996 1965 1267 783
BseYI C'CCAG_C
Cuts at: 2275 7214 7710 2275
Size: 4939 496 2576
Fragments arranged by size:
4939 2576 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2165 2372 2438 2974 4175 4821 5270
Size: 573 429 207 66 536 1201 646 449
Cuts at: 5270 1163
Size: 3904
Fragments arranged by size:
3904 1201 646 573 536 449 429 207
66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3079 5461 6222 7250 1540
Size: 275 186 1078 2382 761 1028 2301
Fragments arranged by size:
2382 2301 1078 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2115 2511 6072 6157 7228 2115
Size: 396 3561 85 1071 2898
Fragments arranged by size:
3561 2898 1071 396 85
BsmI GAATG_Cn'
Cuts at: 5124 5522 5615 5124
Size: 398 93 7520
Fragments arranged by size:
7520 398 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2165 2399 2511 3286 4905 5330
Size: 399 325 50 234 112 775 1619 425
Cuts at: 5330 6072 6157 7228 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1619 1071 775 742 425 399 325
234 112 85 50
BspEI T'CCGG_A
Cuts at: 7674 7674
Size: 8011
BspHI T'CATG_A
Cuts at: 5900 7630 5900
Size: 1730 6281
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3408 4332 5366 844
Size: 2564 924 1034 3489
Fragments arranged by size:
3489 2564 1034 924
BsrBI CCG'CTC
Cuts at: 1394 1823 5898 1394
Size: 429 4075 3507
Fragments arranged by size:
4075 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3930 6506 6680 65
Size: 1183 2682 2576 174 1396
Fragments arranged by size:
2682 2576 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2189 2271 3002 6655 6842 1700
Size: 110 379 82 731 3653 187 2869
Fragments arranged by size:
3653 2869 731 379 187 110 82
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8011
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8011
BssSI C'ACGA_G
Cuts at: 3041 6071 7083 3041
Size: 3030 1012 3969
Fragments arranged by size:
3969 3030 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8011
BstBI TT'CG_AA
Cuts at: 6815 6815
Size: 8011
BstEII G'GTnAC_C
Cuts at: 1184 2545 1184
Size: 1361 6650
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3562 2011
Size: 1551 6460
BstYI r'GATC_y
Cuts at: 1972 2328 2406 3300 4585 5341 5708 6093 6110
Size: 356 78 894 1285 756 367 385 17
Cuts at: 6110 7562 7648 7660 8006 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1285 894 756 385 367 356
346 86 78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2031 2106 2194 2536 2603 3028
Size: 552 939 27 75 88 342 67 425
Cuts at: 3028 3433 513
Size: 405 5091
Fragments arranged by size:
5091 939 552 425 405 342 88 75
67 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7272
Fragments arranged by size:
7272 683 56
BtsI GCAGTG_nn'
Cuts at: 2089 2324 2887 4782 5527 6321 6341 2089
Size: 235 563 1895 745 794 20 3759
Fragments arranged by size:
3759 1895 794 745 563 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8011
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4648 4683 529
Size: 35 4084 35 3857
Fragments arranged by size:
4084 3857 35 35
DraI TTT'AAA
Cuts at: 4450 4501 4711 5315 4450
Size: 51 210 604 7146
Fragments arranged by size:
7146 604 210 51
DraIII CAC_nnn'GTG
Cuts at: 2416 2416
Size: 8011
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7018 809
Size: 507 5702 1802
Fragments arranged by size:
5702 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2187 2191
Size: 54 1425 50 275 18 261 96 4
Cuts at: 2191 2568 2760 2830 2972 3816 3837 5458 6347
Size: 377 192 70 142 844 21 1621 889
Cuts at: 6347 7976 8
Size: 1629 43
Fragments arranged by size:
1629 1621 1425 889 844 377 275 261
192 142 96 70 54 50 43 21
18 4
EagI C'GGCC_G
Cuts at: 1537 1812 5458 1537
Size: 275 3646 4090
Fragments arranged by size:
4090 3646 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2901 2961 5941 1353
Size: 1548 60 2980 3423
Fragments arranged by size:
3423 2980 1548 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2273 2576 6598 6972 7118 387
Size: 1064 138 684 303 4022 374 146 1280
Fragments arranged by size:
4022 1280 1064 684 374 303 146 138
EcoICRI GAG'CTC
Cuts at: 2113 2113
Size: 8011
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2261 2543 2919 3281 3303 3968
Size: 393 401 228 282 376 362 22 665
Cuts at: 3968 4070 4776 6074 6671 7458 7726 1239
Size: 102 706 1298 597 787 268 1524
Fragments arranged by size:
1524 1298 787 706 665 597 401 393
376 362 282 268 228 102 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2951 4165 2951
Size: 1214 6797
EcoRI G'AATT_C
Cuts at: 4330 5320 5698 4330
Size: 990 378 6643
Fragments arranged by size:
6643 990 378
EcoRV GAT'ATC
Cuts at: 3112 3465 3112
Size: 353 7658
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3398 4259 4547 3398
Size: 861 288 6862
Fragments arranged by size:
6862 861 288
FseI GG_CCGG'CC
Cuts at: 2193 2193
Size: 8011
FspI TGC'GCA
Cuts at: 6517 6517
Size: 8011
HaeII r_GCGC'y
Cuts at: 1678 2065 2177 2240 2474 2733 4032 4391 7158
Size: 387 112 63 234 259 1299 359 2767
Cuts at: 7158 1678
Size: 2531
Fragments arranged by size:
2767 2531 1299 387 359 259 234 112
63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3429 3461 4238 4270 4562
Size: 32 135 32 1857 32 777 32 292
Cuts at: 4562 4594 6652 6684 6726 6758 1373
Size: 32 2058 32 42 32 2626
Fragments arranged by size:
2626 2058 1857 777 292 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3882 5130 5355 5601 669
Size: 988 281 1944 1248 225 246 3079
Fragments arranged by size:
3079 1944 1248 988 281 246 225
HindIII A'AGCT_T
Cuts at: 748 3490 4313 4348 748
Size: 2742 823 35 4411
Fragments arranged by size:
4411 2742 823 35
HpaI GTT'AAC
Cuts at: 3882 5130 5601 3882
Size: 1248 471 6292
Fragments arranged by size:
6292 1248 471
KasI G'GCGC_C
Cuts at: 2061 2729 2061
Size: 668 7343
KpnI G_GTAC'C
Cuts at: 5336 5336
Size: 8011
MfeI C'AATT_G
Cuts at: 3665 3830 3989 4395 4485 4665 5610 3665
Size: 165 159 406 90 180 945 6066
Fragments arranged by size:
6066 945 406 180 165 159 90
MluI A'CGCG_T
Cuts at: 7887 7887
Size: 8011
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7125 7309 1305
Size: 5820 184 2007
Fragments arranged by size:
5820 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2570 2762 3818 3839 10
Size: 54 1425 343 738 192 1056 21 4182
Fragments arranged by size:
4182 1425 1056 738 343 192 54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 2514 2756 3045 4184 4509 5969 6328
Size: 648 1348 242 289 1139 325 1460 359
Cuts at: 6328 6487 518
Size: 159 2042
Fragments arranged by size:
2042 1460 1348 1139 648 359 325 289
242 159
MspA1I CmG'CkG
Cuts at: 1606 2422 2458 2977 5427 6104 7252 7497 1606
Size: 816 36 519 2450 677 1148 245 2120
Fragments arranged by size:
2450 2120 1148 816 677 519 245 36
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 8011
NarI GG'CG_CC
Cuts at: 2062 2730 2062
Size: 668 7343
NcoI C'CATG_G
Cuts at: 513 1065 2004 2106 513
Size: 552 939 102 6418
Fragments arranged by size:
6418 939 552 102
NdeI CA'TA_TG
Cuts at: 387 4363 4603 387
Size: 3976 240 3795
Fragments arranged by size:
3976 3795 240
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 8011
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8011
NotI GC'GGCC_GC
Cuts at: 5458 5458
Size: 8011
NspI r_CATG'y
Cuts at: 1132 1229 3457 4531 5369 6914 1132
Size: 97 2228 1074 838 1545 2229
Fragments arranged by size:
2229 2228 1545 1074 838 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8011
PciI A'CATG_T
Cuts at: 3453 6910 3453
Size: 3457 4554
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2275 3835 1108
Size: 1167 1560 5284
Fragments arranged by size:
5284 1560 1167
PfoI T'CCnGG_A
Cuts at: 2625 3894 4369 5157 2625
Size: 1269 475 788 5479
Fragments arranged by size:
5479 1269 788 475
PmeI GTTT'AAAC
Cuts at: 5315 5315
Size: 8011
PmlI CAC'GTG
Cuts at: 2185 2917 2185
Size: 732 7279
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2510 2542 3035 3067 6080 6112 7593 7625 2510
Size: 32 493 32 3013 32 1481 32 2896
Fragments arranged by size:
3013 2896 1481 493 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8011
PsiI TTA'TAA
Cuts at: 5581 5581
Size: 8011
PstI C_TGCA'G
Cuts at: 830 2742 3346 4329 5363 830
Size: 1912 604 983 1034 3478
Fragments arranged by size:
3478 1912 1034 983 604
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8011
SacI G_AGCT'C
Cuts at: 2115 2115
Size: 8011
SalI G'TCGA_C
Cuts at: 1936 5353 1936
Size: 3417 4594
SbfI CC_TGCA'GG
Cuts at: 5363 5363
Size: 8011
ScaI AGT'ACT
Cuts at: 6259 7971 6259
Size: 1712 6299
SexAI A'CCwGG_T
Cuts at: 2343 2412 2844 2343
Size: 69 432 7510
Fragments arranged by size:
7510 432 69
SfcI C'TryA_G
Cuts at: 826 1045 2738 3342 4325 4464 5359 6494 7175
Size: 219 1693 604 983 139 895 1135 681
Cuts at: 7175 7366 826
Size: 191 1471
Fragments arranged by size:
1693 1471 1135 983 895 681 604 219
191 139
SfoI GGC'GCC
Cuts at: 2063 2731 2063
Size: 668 7343
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8011
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8011
SmaI CCC'GGG
Cuts at: 1872 2400 5338 1872
Size: 528 2938 4545
Fragments arranged by size:
4545 2938 528
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2894 3245 3752 4099 4442 6115
Size: 197 926 951 351 507 347 343 1673
Cuts at: 6115 7016 7278 820
Size: 901 262 1553
Fragments arranged by size:
1673 1553 951 926 901 507 351 347
343 262 197
SnaBI TAC'GTA
Cuts at: 493 5802 493
Size: 5309 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8011
SphI G_CATG'C
Cuts at: 1132 5369 1132
Size: 4237 3774
SrfI GCCC'GGGC
Cuts at: 2400 2400
Size: 8011
SspI AAT'ATT
Cuts at: 5 52 4858 5935 5
Size: 47 4806 1077 2081
Fragments arranged by size:
4806 2081 1077 47
StuI AGG'CCT
Cuts at: 1517 3338 1517
Size: 1821 6190
StyI C'CwwG_G
Cuts at: 513 1065 2004 2106 3366 3484 5450 513
Size: 552 939 102 1260 118 1966 3074
Fragments arranged by size:
3074 1966 1260 939 552 118 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2596 2869 3774 6038 6055 6208 6393
Size: 1255 1016 273 905 2264 17 153 185
Cuts at: 6393 6838 325
Size: 445 1498
Fragments arranged by size:
2264 1498 1255 1016 905 445 273 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6257 7969 96
Size: 274 80 33 51 1443 4280 1712 138
Fragments arranged by size:
4280 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2417 2449 2621 2653 3215 3247 5232 5264 7971
Size: 32 172 32 562 32 1985 32 2707
Cuts at: 7971 8003 2417
Size: 32 2425
Fragments arranged by size:
2707 2425 1985 562 172 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8011
XbaI T'CTAG_A
Cuts at: 5347 5347
Size: 8011
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8011
XmaI C'CCGG_G
Cuts at: 1870 2398 5336 1870
Size: 528 2938 4545
Fragments arranged by size:
4545 2938 528
XmnI GAAnn'nnTTC
Cuts at: 2910 4764 6140 2910
Size: 1854 1376 4781
Fragments arranged by size:
4781 1854 1376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2488 2497 2613 4279
Size: 53 83 186 936 954 9 116 1666
Cuts at: 4279 5819 276
Size: 1540 2468
Fragments arranged by size:
2468 1666 1540 954 936 186 116 83
53 9
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoO109I FspAI NruI NsiI
PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SfiI SwaI XcmI