Restriction map for
pFN21AE3349
(Circular) (Six-base) MAPSORT of: pFN21AE3349.seq Check: 704 from: 1 to: 7807
pFN21AE3349 7807 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5617 278
Size: 53 83 186 936 4081 2468
Fragments arranged by size:
4081 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2388 5150 1937
Size: 451 2762 4594
Fragments arranged by size:
4594 2762 451
Acc65I G'GTAC_C
Cuts at: 5128 5128
Size: 7807
AclI AA'CG_TT
Cuts at: 1563 5934 6307 1563
Size: 4371 373 3063
Fragments arranged by size:
4371 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2179 3977 5870 7254 7522 1632
Size: 547 1798 1893 1384 268 1917
Fragments arranged by size:
1917 1893 1798 1384 547 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7610
AflIII A'CryG_T
Cuts at: 1203 2971 6706 7683 1203
Size: 1768 3735 977 1327
Fragments arranged by size:
3735 1768 1327 977
AgeI A'CCGG_T
Cuts at: 6638 6638
Size: 7807
AhdI GACnn_n'nnGTC
Cuts at: 2521 6536 2521
Size: 4015 3792
AleI CACnn'nnGTG
Cuts at: 1166 3745 1166
Size: 2579 5228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2380 4637 1184
Size: 1196 2257 4354
Fragments arranged by size:
4354 2257 1196
AlwNI CAG_nnn'CTG
Cuts at: 3697 4012 4698 4794 5550 7122 3697
Size: 315 686 96 756 1572 4382
Fragments arranged by size:
4382 1572 756 686 315 96
ApaI G_GGCC'C
Cuts at: 2177 3610 4640 2177
Size: 1433 1030 5344
Fragments arranged by size:
5344 1433 1030
ApaLI G'TGCA_C
Cuts at: 2699 5864 7020 2699
Size: 3165 1156 3486
Fragments arranged by size:
3486 3165 1156
ApoI r'AATT_y
Cuts at: 1493 4745 4982 5116 5342 5494 7603 1493
Size: 3252 237 134 226 152 2109 1697
Fragments arranged by size:
3252 2109 1697 237 226 152 134
AseI AT'TA_AT
Cuts at: 160 1032 6361 160
Size: 872 5329 1606
Fragments arranged by size:
5329 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7807
AvaI C'yCGr_G
Cuts at: 1870 1943 4588 4920 5132 1870
Size: 73 2645 332 212 4545
Fragments arranged by size:
4545 2645 332 212 73
BamHI G'GATC_C
Cuts at: 3686 5137 5504 3686
Size: 1451 367 5989
Fragments arranged by size:
5989 1451 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2508 3662 5128 6583
Size: 325 217 627 239 482 1154 1466 1455
Cuts at: 6583 618
Size: 1842
Fragments arranged by size:
1842 1466 1455 1154 627 482 325 239
217
BanII G_rGCy'C
Cuts at: 1391 2177 3167 3610 4020 4140 4640 5126 1391
Size: 786 990 443 410 120 500 486 4072
Fragments arranged by size:
4072 990 786 500 486 443 410 120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3522 3825 928
Size: 971 1623 303 4910
Fragments arranged by size:
4910 1623 971 303
BbvCI CC'TCA_GC
Cuts at: 3728 4554 3728
Size: 826 6981
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5998 6032 1903
Size: 34 50 34 3977 34 3678
Fragments arranged by size:
3977 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5699 6909 5699
Size: 1210 6597
BclI T'GATC_A
Cuts at: 1548 2272 1548
Size: 724 7083
BfrBI ATG'CAT
Cuts at: 4077 4077
Size: 7807
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6418 136
Size: 107 122 71 631 605 4746 1525
Fragments arranged by size:
4746 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3138 4914 7802 3138
Size: 1776 2888 3143
Fragments arranged by size:
3143 2888 1776
BlpI GC'TnA_GC
Cuts at: 5224 5224
Size: 7807
Bme1580I G_kGCm'C
Cuts at: 1790 2177 2703 3610 3665 4640 5868 7024 1790
Size: 387 526 907 55 975 1228 1156 2573
Fragments arranged by size:
2573 1228 1156 975 907 526 387 55
BmgBI CAC'GTC
Cuts at: 1318 2338 5095 1318
Size: 1020 2757 4030
Fragments arranged by size:
4030 2757 1020
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4573 4798 5538
Size: 416 53 83 399 24 3156 225 740
Cuts at: 5538 6496 442
Size: 958 1753
Fragments arranged by size:
3156 1753 958 740 416 399 225 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7807
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4513 4513
Size: 7807
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2196 3637 3909 4034 4144 4429 4483 4929
Size: 957 1441 272 125 110 285 54 446
Cuts at: 4929 6467 1239
Size: 1538 2579
Fragments arranged by size:
2579 1538 1441 957 446 285 272 125
110 54
Bpu10I CC'TnA_GC
Cuts at: 3728 4554 4724 5042 3728
Size: 826 170 318 6493
Fragments arranged by size:
6493 826 318 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3206 3783 4249 5932 6797 7095 3206
Size: 577 466 1683 865 298 3918
Fragments arranged by size:
3918 1683 865 577 466 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4947 6470 882
Size: 617 3448 1523 2219
Fragments arranged by size:
3448 2219 1523 617
BsaAI yAC'GTr
Cuts at: 493 1204 5598 493
Size: 711 4394 2702
Fragments arranged by size:
4394 2702 711
BsaBI GATnn'nnATC
Cuts at: 2985 7801 2985
Size: 4816 2991
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2265 5614 5996 6631
Size: 53 83 186 936 732 3349 382 635
Cuts at: 6631 275
Size: 1451
Fragments arranged by size:
3349 1451 936 732 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6240 6638 6912 7059 7470 6240
Size: 398 274 147 411 6577
Fragments arranged by size:
6577 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2452 2482 4784 4814 1178
Size: 30 165 30 1049 30 2302 30 4171
Fragments arranged by size:
4171 2302 1049 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2090 2108 2111 2114 2117 2174 2473
Size: 840 64 18 3 3 3 57 299
Cuts at: 2473 3977 3980 4429 4432 1186
Size: 1504 3 449 3 4561
Fragments arranged by size:
4561 1504 840 449 299 64 57 18
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2570 3611 3629 3946 4005 7010 7506 2570
Size: 1041 18 317 59 3005 496 2871
Fragments arranged by size:
3005 2871 1041 496 317 59 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2609 2683 1163
Size: 573 873 74 6287
Fragments arranged by size:
6287 873 573 74
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2993 5257 6018 7046 1540
Size: 275 186 992 2264 761 1028 2301
Fragments arranged by size:
2301 2264 1028 992 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2703 3167 5868 5953 7024 2703
Size: 464 2701 85 1071 3486
Fragments arranged by size:
3486 2701 1071 464 85
BsmI GAATG_Cn'
Cuts at: 5318 5411 5318
Size: 93 7714
BsmBI CGTCTCn'nnnn_
Cuts at: 2566 2566
Size: 7807
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2703 3167 3610 3665 4020 4140
Size: 399 387 526 464 443 55 355 120
Cuts at: 4140 4640 5126 5868 5953 7024 1391
Size: 500 486 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 500 486 464 443
399 387 355 120 85 55
BspEI T'CCGG_A
Cuts at: 7470 7470
Size: 7807
BspHI T'CATG_A
Cuts at: 5696 7426 5696
Size: 1730 6077
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4629 5162 844
Size: 3785 533 3489
Fragments arranged by size:
3785 3489 533
BsrBI CCG'CTC
Cuts at: 1394 1823 5694 1394
Size: 429 3871 3507
Fragments arranged by size:
3871 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2146 3735 4042 6302 6476 65
Size: 1183 898 1589 307 2260 174 1396
Fragments arranged by size:
2260 1589 1396 1183 898 307 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4222 4533 6451 6638 1700
Size: 110 2412 311 1918 187 2869
Fragments arranged by size:
2869 2412 1918 311 187 110
BsrGI T'GTAC_A
Cuts at: 96 3362 96
Size: 3266 4541
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7807
BssSI C'ACGA_G
Cuts at: 2761 5867 6879 2761
Size: 3106 1012 3689
Fragments arranged by size:
3689 3106 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2545 2616 4041 794
Size: 1751 71 1425 4560
Fragments arranged by size:
4560 1751 1425 71
BstBI TT'CG_AA
Cuts at: 6611 6611
Size: 7807
BstEII G'GTnAC_C
Cuts at: 1184 2953 3226 1184
Size: 1769 273 5765
Fragments arranged by size:
5765 1769 273
BstXI CCAn_nnnn'nTGG
Cuts at: 3784 4465 3784
Size: 681 7126
BstYI r'GATC_y
Cuts at: 1972 3017 3138 3460 3686 3702 4914 5137 5504
Size: 1045 121 322 226 16 1212 223 367
Cuts at: 5504 5889 5906 7358 7444 7456 7802 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1212 1045 385 367 346 322
226 223 121 86 17 16 12
Bsu36I CC'TnA_GG
Cuts at: 3506 3506
Size: 7807
BtgI C'CryG_G
Cuts at: 513 1065 4131 513
Size: 552 3066 4189
Fragments arranged by size:
4189 3066 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4372 487
Size: 683 56 3146 3922
Fragments arranged by size:
3922 3146 683 56
BtsI GCAGTG_nn'
Cuts at: 3285 4795 5323 6117 6137 3285
Size: 1510 528 794 20 4955
Fragments arranged by size:
4955 1510 794 528 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7807
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7772
DraI TTT'AAA
Cuts at: 5111 5111
Size: 7807
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2684 4223 4879 6814 809
Size: 507 1368 1539 656 1935 1802
Fragments arranged by size:
1935 1802 1539 1368 656 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2990 4704 5254
Size: 54 1425 50 275 18 1160 1714 550
Cuts at: 5254 6143 7772 8
Size: 889 1629 43
Fragments arranged by size:
1714 1629 1425 1160 889 550 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2990 5254 1537
Size: 275 1178 2264 4090
Fragments arranged by size:
4090 2264 1178 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 2085 3861 4601 4959 5737 1353
Size: 714 18 1776 740 358 778 3423
Fragments arranged by size:
3423 1776 778 740 714 358 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6394 6768 6914 387
Size: 1064 138 4805 374 146 1280
Fragments arranged by size:
4805 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3165 3165
Size: 7807
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2179 2196 3637 3909 3977 4034 4144
Size: 393 547 17 1441 272 68 57 110
Cuts at: 4144 4429 4483 4929 5870 6467 7254 7522 1239
Size: 285 54 446 941 597 787 268 1524
Fragments arranged by size:
1524 1441 941 787 597 547 446 393
285 272 268 110 68 57 54 17
EcoNI CCTnn'n_nnAGG
Cuts at: 3509 3959 4243 4444 3509
Size: 450 284 201 6872
Fragments arranged by size:
6872 450 284 201
EcoO109I rG'GnC_Cy
Cuts at: 3307 3606 3607 3950 4450 3307
Size: 299 1 343 500 6664
Fragments arranged by size:
6664 500 343 299 1
EcoRI G'AATT_C
Cuts at: 5116 5494 5116
Size: 378 7429
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4243 4243
Size: 7807
FspI TGC'GCA
Cuts at: 6313 6313
Size: 7807
HaeII r_GCGC'y
Cuts at: 1678 4095 4270 4486 6954 1678
Size: 2417 175 216 2468 2531
Fragments arranged by size:
2531 2468 2417 216 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2264 2266 2296 2507
Size: 32 135 32 662 30 2 30 211
Cuts at: 2507 2539 3860 3892 4007 4039 4784 4816 4961
Size: 32 1321 32 115 32 745 32 145
Cuts at: 4961 4993 6448 6480 6522 6554 1373
Size: 32 1455 32 42 32 2626
Fragments arranged by size:
2626 1455 1321 745 662 211 145 135
115 42 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 5151 5397 669
Size: 988 281 3213 246 3079
Fragments arranged by size:
3213 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7807
HpaI GTT'AAC
Cuts at: 5397 5397
Size: 7807
KpnI G_GTAC'C
Cuts at: 5132 5132
Size: 7807
MfeI C'AATT_G
Cuts at: 2362 5406 2362
Size: 3044 4763
MluI A'CGCG_T
Cuts at: 2971 7683 2971
Size: 4712 3095
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2262 2385 3096 4566 4985 6921 7105 1305
Size: 957 123 711 1470 419 1936 184 2007
Fragments arranged by size:
2007 1936 1470 957 711 419 184 123
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4706 10
Size: 54 1425 343 2874 3111
Fragments arranged by size:
3111 2874 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2706 3197 3745 5765 6124 6283 518
Size: 648 1540 491 548 2020 359 159 2042
Fragments arranged by size:
2042 2020 1540 648 548 491 359 159
MspA1I CmG'CkG
Cuts at: 1606 2065 2112 2384 3259 3615 3652 5223 5900
Size: 459 47 272 875 356 37 1571 677
Cuts at: 5900 7048 7293 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1571 1148 875 677 459 356 272
245 47 37
NaeI GCC'GGC
Cuts at: 4535 4535
Size: 7807
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7255
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7807
NgoMIV G'CCGG_C
Cuts at: 4533 4533
Size: 7807
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7807
NotI GC'GGCC_GC
Cuts at: 5254 5254
Size: 7807
NsiI A_TGCA'T
Cuts at: 4079 4079
Size: 7807
NspI r_CATG'y
Cuts at: 1132 1229 5027 5165 6710 1132
Size: 97 3798 138 1545 2229
Fragments arranged by size:
3798 2229 1545 138 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7807
PciI A'CATG_T
Cuts at: 6706 6706
Size: 7807
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2800 3202 4012 1108
Size: 1692 402 810 4903
Fragments arranged by size:
4903 1692 810 402
PfoI T'CCnGG_A
Cuts at: 2212 2793 2212
Size: 581 7226
PmeI GTTT'AAAC
Cuts at: 5111 5111
Size: 7807
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5876 5908 7389 7421 5876
Size: 32 1481 32 6262
Fragments arranged by size:
6262 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3950 4450 3950
Size: 500 7307
PshAI GACnn'nnGTC
Cuts at: 1613 2384 1613
Size: 771 7036
PsiI TTA'TAA
Cuts at: 5377 5377
Size: 7807
PspOMI G'GGCC_C
Cuts at: 2173 3606 4636 2173
Size: 1433 1030 5344
Fragments arranged by size:
5344 1433 1030
PspXI vC'TCGA_Gb
Cuts at: 4588 4588
Size: 7807
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4804 4836 4804
Size: 32 7775
PstI C_TGCA'G
Cuts at: 830 2133 2538 3702 4101 5159 830
Size: 1303 405 1164 399 1058 3478
Fragments arranged by size:
3478 1303 1164 1058 405 399
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7807
PvuII CAG'CTG
Cuts at: 2065 3259 3615 3652 2065
Size: 1194 356 37 6220
Fragments arranged by size:
6220 1194 356 37
RsrII CG'GwC_CG
Cuts at: 2556 2556
Size: 7807
SacI G_AGCT'C
Cuts at: 3167 3167
Size: 7807
SalI G'TCGA_C
Cuts at: 1936 5149 1936
Size: 3213 4594
SanDI GG'GwC_CC
Cuts at: 3950 4450 3950
Size: 500 7307
SbfI CC_TGCA'GG
Cuts at: 5159 5159
Size: 7807
ScaI AGT'ACT
Cuts at: 6055 7767 6055
Size: 1712 6095
SfcI C'TryA_G
Cuts at: 826 1045 2129 2534 3698 4097 5155 6290 6971
Size: 219 1084 405 1164 399 1058 1135 681
Cuts at: 6971 7162 826
Size: 191 1471
Fragments arranged by size:
1471 1164 1135 1084 1058 681 405 399
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7807
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7807
SmaI CCC'GGG
Cuts at: 1872 4922 5134 1872
Size: 3050 212 4545
Fragments arranged by size:
4545 3050 212
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3221 3798 4228 4588 5911 6812
Size: 197 926 1278 577 430 360 1323 901
Cuts at: 6812 7074 820
Size: 262 1553
Fragments arranged by size:
1553 1323 1278 926 901 577 430 360
262 197
SnaBI TAC'GTA
Cuts at: 493 5598 493
Size: 5105 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7807
SphI G_CATG'C
Cuts at: 1132 5165 1132
Size: 4033 3774
SspI AAT'ATT
Cuts at: 5 52 5731 5
Size: 47 5679 2081
Fragments arranged by size:
5679 2081 47
StuI AGG'CCT
Cuts at: 1517 2424 3125 3872 1517
Size: 907 701 747 5452
Fragments arranged by size:
5452 907 747 701
StyI C'CwwG_G
Cuts at: 513 1065 2431 5246 513
Size: 552 1366 2815 3074
Fragments arranged by size:
3074 2815 1366 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2044 2544 3209 3956 4029 5834 5851
Size: 1255 464 500 665 747 73 1805 17
Cuts at: 5851 6004 6189 6634 325
Size: 153 185 445 1498
Fragments arranged by size:
1805 1498 1255 747 665 500 464 445
185 153 73 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3362 6053 7765
Size: 274 80 33 51 1443 1385 2691 1712
Cuts at: 7765 96
Size: 138
Fragments arranged by size:
2691 1712 1443 1385 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2452 2484 7767 7799 2452
Size: 32 5283 32 2460
Fragments arranged by size:
5283 2460 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4309 4397 1316
Size: 2993 88 4726
Fragments arranged by size:
4726 2993 88
XbaI T'CTAG_A
Cuts at: 5143 5143
Size: 7807
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3199 3199
Size: 7807
XhoI C'TCGA_G
Cuts at: 1943 4588 1943
Size: 2645 5162
XmaI C'CCGG_G
Cuts at: 1870 4920 5132 1870
Size: 3050 212 4545
Fragments arranged by size:
4545 3050 212
XmnI GAAnn'nnTTC
Cuts at: 4816 5936 4816
Size: 1120 6687
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5615 276
Size: 53 83 186 936 4081 2468
Fragments arranged by size:
4081 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI
PacI PmlI SacII SapI SexAI SfiI SfoI SrfI
SwaI