Restriction map for
pFN21AE3348
(Circular) (Six-base) MAPSORT of: pFN21AE3348.seq Check: 2844 from: 1 to: 7801
pFN21AE3348 7801 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2268 3504 3565 3732 2268
Size: 1236 61 167 6337
Fragments arranged by size:
6337 1236 167 61
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4899 5611 278
Size: 53 83 186 936 3363 712 2468
Fragments arranged by size:
3363 2468 936 712 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5144 1937
Size: 3207 4594
Acc65I G'GTAC_C
Cuts at: 2084 5122 2084
Size: 3038 4763
AclI AA'CG_TT
Cuts at: 1563 5928 6301 1563
Size: 4365 373 3063
Fragments arranged by size:
4365 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2174 2827 3122 3220 3385 3414 3691 3802
Size: 542 653 295 98 165 29 277 111
Cuts at: 3802 4091 5864 7248 7516 1632
Size: 289 1773 1384 268 1917
Fragments arranged by size:
1917 1773 1384 653 542 295 289 277
268 165 111 98 29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7604
AflIII A'CryG_T
Cuts at: 1203 6700 7677 1203
Size: 5497 977 1327
Fragments arranged by size:
5497 1327 977
AgeI A'CCGG_T
Cuts at: 3566 6632 3566
Size: 3066 4735
AhdI GACnn_n'nnGTC
Cuts at: 3192 6530 3192
Size: 3338 4463
AleI CACnn'nnGTG
Cuts at: 1166 2642 4518 1166
Size: 1476 1876 4449
Fragments arranged by size:
4449 1876 1476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2042 1184
Size: 858 6943
AlwNI CAG_nnn'CTG
Cuts at: 2566 3190 3505 3556 3976 4420 4876 5019 5544
Size: 624 315 51 420 444 456 143 525
Cuts at: 5544 7116 2566
Size: 1572 3251
Fragments arranged by size:
3251 1572 624 525 456 444 420 315
143 51
ApaI G_GGCC'C
Cuts at: 3811 4245 5026 3811
Size: 434 781 6586
Fragments arranged by size:
6586 781 434
ApaLI G'TGCA_C
Cuts at: 5858 7014 5858
Size: 1156 6645
ApoI r'AATT_y
Cuts at: 1493 5110 5336 5488 7597 1493
Size: 3617 226 152 2109 1697
Fragments arranged by size:
3617 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6355 160
Size: 872 5323 1606
Fragments arranged by size:
5323 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7801
AvaI C'yCGr_G
Cuts at: 1870 1943 2578 5126 1870
Size: 73 635 2548 4545
Fragments arranged by size:
4545 2548 635 73
BamHI G'GATC_C
Cuts at: 5131 5498 5131
Size: 367 7434
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2582 2644 2710 4103
Size: 325 217 627 297 498 62 66 1393
Cuts at: 4103 5122 6577 618
Size: 1019 1455 1842
Fragments arranged by size:
1842 1455 1393 1019 627 498 325 297
217 66 62
BanII G_rGCy'C
Cuts at: 1391 2115 2352 3811 4194 4245 4922 5026 5120
Size: 724 237 1459 383 51 677 104 94
Cuts at: 5120 1391
Size: 4072
Fragments arranged by size:
4072 1459 724 677 383 237 104 94
51
BbeI G_GCGC'C
Cuts at: 2714 2714
Size: 7801
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3402 4621 928
Size: 971 1503 1219 4108
Fragments arranged by size:
4108 1503 1219 971
BbvCI CC'TCA_GC
Cuts at: 2312 2453 3659 3743 4400 2312
Size: 141 1206 84 657 5713
Fragments arranged by size:
5713 1206 657 141 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4129 4163 4240 4274 4879 4913 5992
Size: 34 2192 34 77 34 605 34 1079
Cuts at: 5992 6026 1903
Size: 34 3678
Fragments arranged by size:
3678 2192 1079 605 77 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5693 6903 5693
Size: 1210 6591
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7801
BfrBI ATG'CAT
Cuts at: 2973 2973
Size: 7801
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2496 4304 4926
Size: 107 122 71 631 605 824 1808 622
Cuts at: 4926 6412 136
Size: 1486 1525
Fragments arranged by size:
1808 1525 1486 824 631 622 605 122
107 71
BglII A'GATC_T
Cuts at: 7796 7796
Size: 7801
BlpI GC'TnA_GC
Cuts at: 5218 5218
Size: 7801
Bme1580I G_kGCm'C
Cuts at: 1790 2166 2373 2587 2649 2810 3811 4245 4258
Size: 376 207 214 62 161 1001 434 13
Cuts at: 4258 4350 4503 4714 5026 5862 7018 1790
Size: 92 153 211 312 836 1156 2573
Fragments arranged by size:
2573 1156 1001 836 434 376 312 214
211 207 161 153 92 62 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7801
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3368 3544 3842
Size: 416 53 83 399 24 1951 176 298
Cuts at: 3842 4669 4995 5532 6490 442
Size: 827 326 537 958 1753
Fragments arranged by size:
1951 1753 958 827 537 416 399 326
298 176 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7801
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4462 4462
Size: 7801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2588 2690 3167 3665 4349 4376 4709
Size: 860 489 102 477 498 684 27 333
Cuts at: 4709 4748 4901 6461 1239
Size: 39 153 1560 2579
Fragments arranged by size:
2579 1560 860 684 498 489 477 333
153 102 39 27
Bpu10I CC'TnA_GC
Cuts at: 2312 2393 2453 2852 3512 3659 3743 3884 4199
Size: 81 60 399 660 147 84 141 315
Cuts at: 4199 4400 4916 2312
Size: 201 516 5197
Fragments arranged by size:
5197 660 516 399 315 201 147 141
84 81 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2270 2606 4199 5926 6791 7089 2270
Size: 336 1593 1727 865 298 2982
Fragments arranged by size:
2982 1727 1593 865 336 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3140 3849 4844 6464 882
Size: 617 1641 709 995 1620 2219
Fragments arranged by size:
2219 1641 1620 995 709 617
BsaAI yAC'GTr
Cuts at: 493 1204 3262 4945 5592 493
Size: 711 2058 1683 647 2702
Fragments arranged by size:
2702 2058 1683 711 647
BsaBI GATnn'nnATC
Cuts at: 7795 7795
Size: 7801
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2711 4896 5608 5990
Size: 53 83 186 936 1178 2185 712 382
Cuts at: 5990 6625 275
Size: 635 1451
Fragments arranged by size:
2185 1451 1178 936 712 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3566 4113 5070 6234 6632 6906 7053 7464 3566
Size: 547 957 1164 398 274 147 411 3903
Fragments arranged by size:
3903 1164 957 547 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7576
Fragments arranged by size:
7576 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4706 4973 1186
Size: 3520 267 4014
Fragments arranged by size:
4014 3520 267
BseYI C'CCAG_C
Cuts at: 2264 2351 2823 2923 3222 3386 3483 3780 4358
Size: 87 472 100 299 164 97 297 578
Cuts at: 4358 4524 4707 4866 5025 5029 7004 7500 2264
Size: 166 183 159 159 4 1975 496 2565
Fragments arranged by size:
2565 1975 578 496 472 299 297 183
166 164 159 159 100 97 87 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2012 2069 3485 3851 3908 4274 4355
Size: 573 276 57 1416 366 57 366 81
Cuts at: 4355 1163
Size: 4609
Fragments arranged by size:
4609 1416 573 366 366 276 81 57
57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4930 5097 5251 6012 7040 1540
Size: 275 186 2929 167 154 761 1028 2301
Fragments arranged by size:
2929 2301 1028 761 275 186 167 154
BsiHKAI G_wGCw'C
Cuts at: 2115 2751 3621 4194 5862 5947 7018 2115
Size: 636 870 573 1668 85 1071 2898
Fragments arranged by size:
2898 1668 1071 870 636 573 85
BsmI GAATG_Cn'
Cuts at: 2908 5312 5405 2908
Size: 2404 93 5304
Fragments arranged by size:
5304 2404 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3986 3986
Size: 7801
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2166 2352 2373 2587 2649 2751
Size: 399 325 51 186 21 214 62 102
Cuts at: 2751 2810 3621 3811 4194 4245 4258 4350 4503
Size: 59 811 190 383 51 13 92 153
Cuts at: 4503 4714 4922 5026 5120 5862 5947 7018 1391
Size: 211 208 104 94 742 85 1071 2174
Fragments arranged by size:
2174 1071 811 742 399 383 325 214
211 208 190 186 153 104 102 94
92 85 62 59 51 51 21 13
BspEI T'CCGG_A
Cuts at: 4113 7464 4113
Size: 3351 4450
BspHI T'CATG_A
Cuts at: 2409 5690 7420 2409
Size: 3281 1730 2790
Fragments arranged by size:
3281 2790 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2268 2664 3504 3565 3732 4428 5156 844
Size: 1424 396 840 61 167 696 728 3489
Fragments arranged by size:
3489 1424 840 728 696 396 167 61
BsrBI CCG'CTC
Cuts at: 1394 1823 3135 3888 4935 5688 1394
Size: 429 1312 753 1047 753 3507
Fragments arranged by size:
3507 1312 1047 753 753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2200 3832 6296 6470 65
Size: 1183 952 1632 2464 174 1396
Fragments arranged by size:
2464 1632 1396 1183 952 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2302 2495 3566 6445 6632 1700
Size: 110 492 193 1071 2879 187 2869
Fragments arranged by size:
2879 2869 1071 492 193 187 110
BsrGI T'GTAC_A
Cuts at: 96 4665 96
Size: 4569 3232
BssHII G'CGCG_C
Cuts at: 1925 4846 1925
Size: 2921 4880
BssSI C'ACGA_G
Cuts at: 3614 5861 6873 3614
Size: 2247 1012 4542
Fragments arranged by size:
4542 2247 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2509 2805 3505 4876 794
Size: 1715 296 700 1371 3719
Fragments arranged by size:
3719 1715 1371 700 296
BstBI TT'CG_AA
Cuts at: 6605 6605
Size: 7801
BstEII G'GTnAC_C
Cuts at: 1184 3457 1184
Size: 2273 5528
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2074 3680 5003 2062
Size: 12 1606 1323 4860
Fragments arranged by size:
4860 1606 1323 12
BstYI r'GATC_y
Cuts at: 1972 3436 5131 5498 5883 5900 7352 7438 7450
Size: 1464 1695 367 385 17 1452 86 12
Cuts at: 7450 7796 1972
Size: 346 1977
Fragments arranged by size:
1977 1695 1464 1452 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2715 2715
Size: 7801
BtgI C'CryG_G
Cuts at: 513 1065 2004 2122 2175 2188 2917 3312 4699
Size: 552 939 118 53 13 729 395 1387
Cuts at: 4699 5091 513
Size: 392 3223
Fragments arranged by size:
3223 1387 939 729 552 395 392 118
53 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7062
Fragments arranged by size:
7062 683 56
BtsI GCAGTG_nn'
Cuts at: 3506 4291 4656 5317 6111 6131 3506
Size: 785 365 661 794 20 5176
Fragments arranged by size:
5176 794 785 661 365 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7801
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7766
DraI TTT'AAA
Cuts at: 5105 5105
Size: 7801
DraIII CAC_nnn'GTG
Cuts at: 2189 2189
Size: 7801
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6808 809
Size: 507 5492 1802
Fragments arranged by size:
5492 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2724 3158
Size: 54 1425 50 275 18 234 660 434
Cuts at: 3158 4927 5094 5248 6137 7766 8
Size: 1769 167 154 889 1629 43
Fragments arranged by size:
1769 1629 1425 889 660 434 275 234
167 154 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4927 5094 5248 1537
Size: 275 3115 167 154 4090
Fragments arranged by size:
4090 3115 275 167 154
EarI CTCTTCn'nnn_
Cuts at: 1353 2376 5731 1353
Size: 1023 3355 3423
Fragments arranged by size:
3423 3355 1023
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2775 4706 6388 6762 6908 387
Size: 1064 138 1186 1931 1682 374 146 1280
Fragments arranged by size:
1931 1682 1280 1186 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2113 4192 2113
Size: 2079 5722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2174 2588 2690 2827 3122 3167
Size: 393 467 75 414 102 137 295 45
Cuts at: 3167 3220 3385 3414 3665 3691 3802 4091 4349
Size: 53 165 29 251 26 111 289 258
Cuts at: 4349 4376 4709 4748 4901 5864 6461 7248 7516
Size: 27 333 39 153 963 597 787 268
Cuts at: 7516 1239
Size: 1524
Fragments arranged by size:
1524 963 787 597 467 414 393 333
295 289 268 258 251 165 153 137
111 102 75 53 45 39 29 27
26
EcoNI CCTnn'n_nnAGG
Cuts at: 2048 4328 4436 4880 2048
Size: 2280 108 444 4969
Fragments arranged by size:
4969 2280 444 108
EcoO109I rG'GnC_Cy
Cuts at: 2719 2998 3240 3358 3649 3808 4075 4242 4396
Size: 279 242 118 291 159 267 167 154
Cuts at: 4396 4417 4441 4528 5087 2719
Size: 21 24 87 559 5433
Fragments arranged by size:
5433 559 291 279 267 242 167 159
154 118 87 24 21
EcoRI G'AATT_C
Cuts at: 5110 5488 5110
Size: 378 7423
FspI TGC'GCA
Cuts at: 3014 3728 3950 6307 3014
Size: 714 222 2357 4508
Fragments arranged by size:
4508 2357 714 222
HaeII r_GCGC'y
Cuts at: 1678 2025 2714 4206 4281 4708 4876 4963 6948
Size: 347 689 1492 75 427 168 87 1985
Cuts at: 6948 1678
Size: 2531
Fragments arranged by size:
2531 1985 1492 689 427 347 168 87
75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3178 3210 6442 6474 6516
Size: 32 135 32 1606 32 3232 32 42
Cuts at: 6516 6548 1373
Size: 32 2626
Fragments arranged by size:
3232 2626 1606 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5145 5391 669
Size: 988 281 3207 246 3079
Fragments arranged by size:
3207 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3017 748
Size: 2269 5532
HpaI GTT'AAC
Cuts at: 5391 5391
Size: 7801
KasI G'GCGC_C
Cuts at: 2710 2710
Size: 7801
KpnI G_GTAC'C
Cuts at: 2088 5126 2088
Size: 3038 4763
MfeI C'AATT_G
Cuts at: 5400 5400
Size: 7801
MluI A'CGCG_T
Cuts at: 7677 7677
Size: 7801
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 3252 6915 7099 1305
Size: 939 1008 3663 184 2007
Fragments arranged by size:
3663 2007 1008 939 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2066 2726 10
Size: 54 1425 343 234 660 5085
Fragments arranged by size:
5085 1425 660 343 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2238 2414 2471 2642 4518 5759 6118
Size: 648 1072 176 57 171 1876 1241 359
Cuts at: 6118 6277 518
Size: 159 2042
Fragments arranged by size:
2042 1876 1241 1072 648 359 176 171
159 57
MspA1I CmG'CkG
Cuts at: 1606 2035 2775 3010 3288 3562 3822 4069 4131
Size: 429 740 235 278 274 260 247 62
Cuts at: 4131 4687 5029 5217 5894 7042 7287 1606
Size: 556 342 188 677 1148 245 2120
Fragments arranged by size:
2120 1148 740 677 556 429 342 278
274 260 247 245 235 188 62
NaeI GCC'GGC
Cuts at: 2497 2497
Size: 7801
NarI GG'CG_CC
Cuts at: 2711 2711
Size: 7801
NcoI C'CATG_G
Cuts at: 513 1065 2004 2175 3312 513
Size: 552 939 171 1137 5002
Fragments arranged by size:
5002 1137 939 552 171
NdeI CA'TA_TG
Cuts at: 387 3840 387
Size: 3453 4348
NgoMIV G'CCGG_C
Cuts at: 2495 2495
Size: 7801
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7801
NotI GC'GGCC_GC
Cuts at: 5248 5248
Size: 7801
NsiI A_TGCA'T
Cuts at: 2975 2975
Size: 7801
NspI r_CATG'y
Cuts at: 1132 1229 2507 3118 3688 5159 6704 1132
Size: 97 1278 611 570 1471 1545 2229
Fragments arranged by size:
2229 1545 1471 1278 611 570 97
PasI CC'CwG_GG
Cuts at: 1348 2080 1348
Size: 732 7069
PciI A'CATG_T
Cuts at: 6700 6700
Size: 7801
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2128 1108
Size: 1020 6781
PmeI GTTT'AAAC
Cuts at: 5105 5105
Size: 7801
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5870 5902 7383 7415 5870
Size: 32 1481 32 6256
Fragments arranged by size:
6256 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2719 3358 4417 4528 5087 2719
Size: 639 1059 111 559 5433
Fragments arranged by size:
5433 1059 639 559 111
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7801
PsiI TTA'TAA
Cuts at: 5371 5371
Size: 7801
PspOMI G'GGCC_C
Cuts at: 3807 4241 5022 3807
Size: 434 781 6586
Fragments arranged by size:
6586 781 434
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2948 2980 2948
Size: 32 7769
PstI C_TGCA'G
Cuts at: 830 2558 3201 3351 3416 3501 3576 3794 5153
Size: 1728 643 150 65 85 75 218 1359
Cuts at: 5153 830
Size: 3478
Fragments arranged by size:
3478 1728 1359 643 218 150 85 75
65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7801
PvuII CAG'CTG
Cuts at: 3010 4069 4131 4687 5029 3010
Size: 1059 62 556 342 5782
Fragments arranged by size:
5782 1059 556 342 62
RsrII CG'GwC_CG
Cuts at: 4825 4825
Size: 7801
SacI G_AGCT'C
Cuts at: 2115 4194 2115
Size: 2079 5722
SalI G'TCGA_C
Cuts at: 1936 5143 1936
Size: 3207 4594
SanDI GG'GwC_CC
Cuts at: 5087 5087
Size: 7801
SapI GCTCTTCn'nnn_
Cuts at: 2376 2376
Size: 7801
SbfI CC_TGCA'GG
Cuts at: 2558 5153 2558
Size: 2595 5206
ScaI AGT'ACT
Cuts at: 6049 7761 6049
Size: 1712 6089
SexAI A'CCwGG_T
Cuts at: 3453 3453
Size: 7801
SfcI C'TryA_G
Cuts at: 826 1045 2554 3197 3347 3412 3497 3572 3790
Size: 219 1509 643 150 65 85 75 218
Cuts at: 3790 3968 5149 6284 6965 7156 826
Size: 178 1181 1135 681 191 1471
Fragments arranged by size:
1509 1471 1181 1135 681 643 219 218
191 178 150 85 75 65
SfoI GGC'GCC
Cuts at: 2712 2712
Size: 7801
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7801
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7801
SmaI CCC'GGG
Cuts at: 1872 2580 5128 1872
Size: 708 2548 4545
Fragments arranged by size:
4545 2548 708
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2285 2621 4214 5905 6806 7068
Size: 197 926 342 336 1593 1691 901 262
Cuts at: 7068 820
Size: 1553
Fragments arranged by size:
1691 1593 1553 926 901 342 336 262
197
SnaBI TAC'GTA
Cuts at: 493 5592 493
Size: 5099 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7801
SphI G_CATG'C
Cuts at: 1132 2507 3688 5159 1132
Size: 1375 1181 1471 3774
Fragments arranged by size:
3774 1471 1375 1181
SspI AAT'ATT
Cuts at: 5 52 5725 5
Size: 47 5673 2081
Fragments arranged by size:
5673 2081 47
StuI AGG'CCT
Cuts at: 1517 2868 2990 3882 1517
Size: 1351 122 892 5436
Fragments arranged by size:
5436 1351 892 122
StyI C'CwwG_G
Cuts at: 513 1065 2004 2175 2590 2985 3312 3432 3877
Size: 552 939 171 415 395 327 120 445
Cuts at: 3877 4170 4176 5240 513
Size: 293 6 1064 3074
Fragments arranged by size:
3074 1064 939 552 445 415 395 327
293 171 120 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2624 4484 4651 4771 5828 5845 5998
Size: 1255 1044 1860 167 120 1057 17 153
Cuts at: 5998 6183 6628 325
Size: 185 445 1498
Fragments arranged by size:
1860 1498 1255 1057 1044 445 185 167
153 120 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2625 4665 6047
Size: 274 80 33 51 1443 648 2040 1382
Cuts at: 6047 7759 96
Size: 1712 138
Fragments arranged by size:
2040 1712 1443 1382 648 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7761 7793 7761
Size: 32 7769
Tth111I GACn'n_nGTC
Cuts at: 1316 4829 1316
Size: 3513 4288
XbaI T'CTAG_A
Cuts at: 5137 5137
Size: 7801
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2359 2797 4235 4858 5000 2359
Size: 438 1438 623 142 5160
Fragments arranged by size:
5160 1438 623 438 142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7801
XmaI C'CCGG_G
Cuts at: 1870 2578 5126 1870
Size: 708 2548 4545
Fragments arranged by size:
4545 2548 708
XmnI GAAnn'nnTTC
Cuts at: 5930 5930
Size: 7801
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4897 5609 276
Size: 53 83 186 936 3363 712 2468
Fragments arranged by size:
3363 2468 936 712 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BstZ17I EcoRV
FalI FseI FspAI NruI PacI PfoI PmlI PspXI
SacII SfiI SrfI SwaI