Restriction map for
pFN21AE3347
(Circular) (Six-base) MAPSORT of: pFN21AE3347.seq Check: 8977 from: 1 to: 7786
pFN21AE3347 7786 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2770 2770
Size: 7786
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5596 278
Size: 53 83 186 936 4060 2468
Fragments arranged by size:
4060 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2491 5129 1937
Size: 554 2638 4594
Fragments arranged by size:
4594 2638 554
Acc65I G'GTAC_C
Cuts at: 5107 5107
Size: 7786
AclI AA'CG_TT
Cuts at: 1563 5913 6286 1563
Size: 4350 373 3063
Fragments arranged by size:
4350 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2949 4304 4349 5849 7233 7501 1632
Size: 1317 1355 45 1500 1384 268 1917
Fragments arranged by size:
1917 1500 1384 1355 1317 268 45
AfeI AGC'GCT
Cuts at: 2846 3879 2846
Size: 1033 6753
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7589
AflIII A'CryG_T
Cuts at: 1203 2471 6685 7662 1203
Size: 1268 4214 977 1327
Fragments arranged by size:
4214 1327 1268 977
AgeI A'CCGG_T
Cuts at: 6617 6617
Size: 7786
AhdI GACnn_n'nnGTC
Cuts at: 6515 6515
Size: 7786
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7786
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7786
AlwNI CAG_nnn'CTG
Cuts at: 2456 3911 5529 7101 2456
Size: 1455 1618 1572 3141
Fragments arranged by size:
3141 1618 1572 1455
ApaI G_GGCC'C
Cuts at: 2308 2308
Size: 7786
ApaLI G'TGCA_C
Cuts at: 5843 6999 5843
Size: 1156 6630
ApoI r'AATT_y
Cuts at: 1493 2881 3307 3627 3743 3994 4987 5095 5321
Size: 1388 426 320 116 251 993 108 226
Cuts at: 5321 5473 7582 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1388 993 426 320 251 226
152 116 108
AseI AT'TA_AT
Cuts at: 160 1032 4779 6340 160
Size: 872 3747 1561 1606
Fragments arranged by size:
3747 1606 1561 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7786
AvaI C'yCGr_G
Cuts at: 1870 1943 3805 4095 5111 1870
Size: 73 1862 290 1016 4545
Fragments arranged by size:
4545 1862 1016 290 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4848 4881 4848
Size: 33 7753
BamHI G'GATC_C
Cuts at: 5116 5483 5116
Size: 367 7419
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2555 3964 5016 5107 6562
Size: 325 217 627 768 1409 1052 91 1455
Cuts at: 6562 618
Size: 1842
Fragments arranged by size:
1842 1455 1409 1052 768 627 325 217
91
BanII G_rGCy'C
Cuts at: 1391 2308 3364 4496 5105 1391
Size: 917 1056 1132 609 4072
Fragments arranged by size:
4072 1132 1056 917 609
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3843 928
Size: 971 1944 4871
Fragments arranged by size:
4871 1944 971
BbvCI CC'TCA_GC
Cuts at: 3274 3843 3889 3274
Size: 569 46 7171
Fragments arranged by size:
7171 569 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5977 6011 1903
Size: 34 4040 34 3678
Fragments arranged by size:
4040 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5678 6888 5678
Size: 1210 6576
BclI T'GATC_A
Cuts at: 1548 2641 4147 1548
Size: 1093 1506 5187
Fragments arranged by size:
5187 1506 1093
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6397 136
Size: 107 122 71 631 605 4725 1525
Fragments arranged by size:
4725 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3145 4048 7781 3145
Size: 903 3733 3150
Fragments arranged by size:
3733 3150 903
BlpI GC'TnA_GC
Cuts at: 5203 5203
Size: 7786
Bme1580I G_kGCm'C
Cuts at: 1790 2139 2308 2323 4684 5847 7003 1790
Size: 349 169 15 2361 1163 1156 2573
Fragments arranged by size:
2573 2361 1163 1156 349 169 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7786
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5517 6475 442
Size: 416 53 83 399 24 4100 958 1753
Fragments arranged by size:
4100 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3042 1056
Size: 1986 5800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4121 4641 4121
Size: 520 7266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 3057 3336 3597 3811 4167 4543 6446
Size: 815 1003 279 261 214 356 376 1903
Cuts at: 6446 1239
Size: 2579
Fragments arranged by size:
2579 1903 1003 815 376 356 279 261
214
Bpu10I CC'TnA_GC
Cuts at: 2977 3128 3274 3572 3843 3889 4636 2977
Size: 151 146 298 271 46 747 6127
Fragments arranged by size:
6127 747 298 271 151 146 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3036 5911 6776 7074 3036
Size: 2875 865 298 3748
Fragments arranged by size:
3748 2875 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2014 2050 6449 882
Size: 617 515 36 4399 2219
Fragments arranged by size:
4399 2219 617 515 36
BsaAI yAC'GTr
Cuts at: 493 1204 5577 493
Size: 711 4373 2702
Fragments arranged by size:
4373 2702 711
BsaBI GATnn'nnATC
Cuts at: 2682 4047 7780 2682
Size: 1365 3733 2688
Fragments arranged by size:
3733 2688 1365
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5593 5975 6610 275
Size: 53 83 186 936 4060 382 635 1451
Fragments arranged by size:
4060 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6219 6617 6891 7038 7449 6219
Size: 398 274 147 411 6556
Fragments arranged by size:
6556 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2030 2060 3155 3185 4205
Size: 30 165 30 627 30 1095 30 1020
Cuts at: 4205 4235 4541 4571 4588 4618 1178
Size: 30 306 30 17 30 4346
Fragments arranged by size:
4346 1095 1020 627 306 165 30 30
30 30 30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7786
BseYI C'CCAG_C
Cuts at: 2103 4610 6989 7485 2103
Size: 2507 2379 496 2404
Fragments arranged by size:
2507 2404 2379 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2847 2883 3128 1163
Size: 573 1111 36 245 5821
Fragments arranged by size:
5821 1111 573 245 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2020 5236 5997 7025 1540
Size: 275 186 19 3216 761 1028 2301
Fragments arranged by size:
3216 2301 1028 761 275 186 19
BsiHKAI G_wGCw'C
Cuts at: 5847 5932 7003 5847
Size: 85 1071 6630
Fragments arranged by size:
6630 1071 85
BsmI GAATG_Cn'
Cuts at: 3694 4284 4381 4701 5297 5390 3694
Size: 590 97 320 596 93 6090
Fragments arranged by size:
6090 596 590 320 97 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4431 4431
Size: 7786
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 2308 2323 3364 4496 4684 5105
Size: 399 349 169 15 1041 1132 188 421
Cuts at: 5105 5847 5932 7003 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1132 1071 1041 742 421 399 349
188 169 85 15
BspEI T'CCGG_A
Cuts at: 7449 7449
Size: 7786
BspHI T'CATG_A
Cuts at: 2686 5675 7405 2686
Size: 2989 1730 3067
Fragments arranged by size:
3067 2989 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2770 4648 5141 844
Size: 1926 1878 493 3489
Fragments arranged by size:
3489 1926 1878 493
BsrBI CCG'CTC
Cuts at: 1394 1823 5673 1394
Size: 429 3850 3507
Fragments arranged by size:
3850 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3566 3772 6281 6455 65
Size: 1183 2318 206 2509 174 1396
Fragments arranged by size:
2509 2318 1396 1183 206 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2019 6430 6617 1700
Size: 110 209 4411 187 2869
Fragments arranged by size:
4411 2869 209 187 110
BsrGI T'GTAC_A
Cuts at: 96 3199 96
Size: 3103 4683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7786
BssSI C'ACGA_G
Cuts at: 5846 6858 5846
Size: 1012 6774
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3524 3911 4457 794
Size: 2730 387 546 4123
Fragments arranged by size:
4123 2730 546 387
BstBI TT'CG_AA
Cuts at: 6590 6590
Size: 7786
BstEII G'GTnAC_C
Cuts at: 1184 4763 1184
Size: 3579 4207
BstXI CCAn_nnnn'nTGG
Cuts at: 2270 2576 2270
Size: 306 7480
BstYI r'GATC_y
Cuts at: 1972 2624 3145 3809 3947 4048 5116 5483 5868
Size: 652 521 664 138 101 1068 367 385
Cuts at: 5868 5885 7337 7423 7435 7781 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1068 664 652 521 385 367
346 138 101 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4915 4915
Size: 7786
BtgI C'CryG_G
Cuts at: 513 1065 2004 2198 5061 513
Size: 552 939 194 2863 3238
Fragments arranged by size:
3238 2863 939 552 194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7047
Fragments arranged by size:
7047 683 56
BtsI GCAGTG_nn'
Cuts at: 3085 4226 4740 5302 6096 6116 3085
Size: 1141 514 562 794 20 4755
Fragments arranged by size:
4755 1141 794 562 514 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7786
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7751
DraI TTT'AAA
Cuts at: 2280 4800 5090 2280
Size: 2520 290 4976
Fragments arranged by size:
4976 2520 290
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2938 6793 809
Size: 507 1622 3855 1802
Fragments arranged by size:
3855 1802 1622 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5233 6122 7751
Size: 54 1425 50 275 18 3403 889 1629
Cuts at: 7751 8
Size: 43
Fragments arranged by size:
3403 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5233 1537
Size: 275 3421 4090
Fragments arranged by size:
4090 3421 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2431 4536 5716 1353
Size: 1078 2105 1180 3423
Fragments arranged by size:
3423 2105 1180 1078
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6373 6747 6893 387
Size: 1064 138 4784 374 146 1280
Fragments arranged by size:
4784 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2949 3057 3336 3597 3811 4167
Size: 393 422 895 108 279 261 214 356
Cuts at: 4167 4304 4349 4543 5849 6446 7233 7501 1239
Size: 137 45 194 1306 597 787 268 1524
Fragments arranged by size:
1524 1306 895 787 597 422 393 356
279 268 261 214 194 137 108 45
EcoNI CCTnn'n_nnAGG
Cuts at: 3345 4500 3345
Size: 1155 6631
EcoRI G'AATT_C
Cuts at: 5095 5473 5095
Size: 378 7408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2676 2676
Size: 7786
FspI TGC'GCA
Cuts at: 6292 6292
Size: 7786
HaeII r_GCGC'y
Cuts at: 1678 2848 3881 6933 1678
Size: 1170 1033 3052 2531
Fragments arranged by size:
3052 2531 1170 1033
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 2648 2680 3149
Size: 32 135 32 458 32 586 32 469
Cuts at: 3149 3181 3939 3971 4541 4573 6427 6459 6501
Size: 32 758 32 570 32 1854 32 42
Cuts at: 6501 6533 1373
Size: 32 2626
Fragments arranged by size:
2626 1854 758 586 570 469 458 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2492 5130 5376 669
Size: 988 281 554 2638 246 3079
Fragments arranged by size:
3079 2638 988 554 281 246
HindIII A'AGCT_T
Cuts at: 748 2163 3298 748
Size: 1415 1135 5236
Fragments arranged by size:
5236 1415 1135
HpaI GTT'AAC
Cuts at: 5376 5376
Size: 7786
KpnI G_GTAC'C
Cuts at: 5111 5111
Size: 7786
MfeI C'AATT_G
Cuts at: 2370 5385 2370
Size: 3015 4771
MluI A'CGCG_T
Cuts at: 7662 7662
Size: 7786
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4424 6900 7084 1305
Size: 3119 2476 184 2007
Fragments arranged by size:
3119 2476 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5964
Fragments arranged by size:
5964 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2268 3978 5744 6103 6262 518
Size: 648 1102 1710 1766 359 159 2042
Fragments arranged by size:
2042 1766 1710 1102 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2119 3278 3524 3893 5202 5879 7027 7272
Size: 513 1159 246 369 1309 677 1148 245
Cuts at: 7272 1606
Size: 2120
Fragments arranged by size:
2120 1309 1159 1148 677 513 369 246
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 5061 513
Size: 552 939 3057 3238
Fragments arranged by size:
3238 3057 939 552
NdeI CA'TA_TG
Cuts at: 387 2638 3975 387
Size: 2251 1337 4198
Fragments arranged by size:
4198 2251 1337
NheI G'CTAG_C
Cuts at: 1052 3038 1052
Size: 1986 5800
NotI GC'GGCC_GC
Cuts at: 5233 5233
Size: 7786
NspI r_CATG'y
Cuts at: 1132 1229 2475 2514 5144 6689 1132
Size: 97 1246 39 2630 1545 2229
Fragments arranged by size:
2630 2229 1545 1246 97 39
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7786
PciI A'CATG_T
Cuts at: 2471 6685 2471
Size: 4214 3572
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7786
PfoI T'CCnGG_A
Cuts at: 2886 3016 2886
Size: 130 7656
PmeI GTTT'AAAC
Cuts at: 5090 5090
Size: 7786
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5855 5887 7368 7400 5855
Size: 32 1481 32 6241
Fragments arranged by size:
6241 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4820 1613
Size: 3207 4579
PsiI TTA'TAA
Cuts at: 2403 3759 5356 2403
Size: 1356 1597 4833
Fragments arranged by size:
4833 1597 1356
PspOMI G'GGCC_C
Cuts at: 2304 2304
Size: 7786
PstI C_TGCA'G
Cuts at: 830 2147 3529 3555 3907 4735 5138 830
Size: 1317 1382 26 352 828 403 3478
Fragments arranged by size:
3478 1382 1317 828 403 352 26
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7786
PvuII CAG'CTG
Cuts at: 3278 3524 3893 3278
Size: 246 369 7171
Fragments arranged by size:
7171 369 246
SalI G'TCGA_C
Cuts at: 1936 2490 5128 1936
Size: 554 2638 4594
Fragments arranged by size:
4594 2638 554
SbfI CC_TGCA'GG
Cuts at: 5138 5138
Size: 7786
ScaI AGT'ACT
Cuts at: 3817 6034 7746 3817
Size: 2217 1712 3857
Fragments arranged by size:
3857 2217 1712
SfcI C'TryA_G
Cuts at: 826 1045 2143 2790 3342 3513 3525 3551 3903
Size: 219 1098 647 552 171 12 26 352
Cuts at: 3903 4731 5134 6269 6950 7141 826
Size: 828 403 1135 681 191 1471
Fragments arranged by size:
1471 1135 1098 828 681 647 552 403
352 219 191 171 26 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7786
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7786
SmaI CCC'GGG
Cuts at: 1872 5113 1872
Size: 3241 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3051 5890 6791 7053 820
Size: 197 926 1108 2839 901 262 1553
Fragments arranged by size:
2839 1553 1108 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5577 493
Size: 5084 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7786
SphI G_CATG'C
Cuts at: 1132 5144 1132
Size: 4012 3774
SspI AAT'ATT
Cuts at: 5 52 5710 5
Size: 47 5658 2081
Fragments arranged by size:
5658 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7786
StyI C'CwwG_G
Cuts at: 513 1065 2004 5061 5225 513
Size: 552 939 3057 164 3074
Fragments arranged by size:
3074 3057 939 552 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4727 4868 5813 5830 5983 6168 6613
Size: 1255 3147 141 945 17 153 185 445
Cuts at: 6613 325
Size: 1498
Fragments arranged by size:
3147 1498 1255 945 445 185 153 141
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3199 3815 5008
Size: 274 80 33 51 1443 1222 616 1193
Cuts at: 5008 6032 7744 96
Size: 1024 1712 138
Fragments arranged by size:
1712 1443 1222 1193 1024 616 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3012 3044 3090 3122 4205 4237 7746 7778 3012
Size: 32 46 32 1083 32 3509 32 3020
Fragments arranged by size:
3509 3020 1083 46 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2042 1316
Size: 726 7060
XbaI T'CTAG_A
Cuts at: 5122 5122
Size: 7786
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7786
XmaI C'CCGG_G
Cuts at: 1870 5111 1870
Size: 3241 4545
XmnI GAAnn'nnTTC
Cuts at: 4362 5915 4362
Size: 1553 6233
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5594 276
Size: 53 83 186 936 4060 2468
Fragments arranged by size:
4060 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I DraIII
EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI