Restriction map for
pFN21AE3345
(Circular) (Six-base) MAPSORT of: pFN21AE3345.seq Check: 7693 from: 1 to: 7762
pFN21AE3345 7762 bp
With 182 enzymes: SgfI *
March 28, 2012 12:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5572 278
Size: 53 83 186 936 4036 2468
Fragments arranged by size:
4036 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3144 5105 1937
Size: 1207 1961 4594
Fragments arranged by size:
4594 1961 1207
Acc65I G'GTAC_C
Cuts at: 5083 5083
Size: 7762
AclI AA'CG_TT
Cuts at: 1563 5889 6262 1563
Size: 4326 373 3063
Fragments arranged by size:
4326 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2223 2295 2574 2743 3092 4377 5825 7209
Size: 591 72 279 169 349 1285 1448 1384
Cuts at: 7209 7477 1632
Size: 268 1917
Fragments arranged by size:
1917 1448 1384 1285 591 349 279 268
169 72
AflII C'TTAA_G
Cuts at: 820 1017 2615 2779 820
Size: 197 1598 164 5803
Fragments arranged by size:
5803 1598 197 164
AflIII A'CryG_T
Cuts at: 1203 3351 6661 7638 1203
Size: 2148 3310 977 1327
Fragments arranged by size:
3310 2148 1327 977
AgeI A'CCGG_T
Cuts at: 6593 6593
Size: 7762
AhdI GACnn_n'nnGTC
Cuts at: 6491 6491
Size: 7762
AleI CACnn'nnGTG
Cuts at: 1166 2781 1166
Size: 1615 6147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2653 1184
Size: 1469 6293
AlwNI CAG_nnn'CTG
Cuts at: 2920 3016 3038 3346 4528 5505 7077 2920
Size: 96 22 308 1182 977 1572 3605
Fragments arranged by size:
3605 1572 1182 977 308 96 22
ApaI G_GGCC'C
Cuts at: 2152 2152
Size: 7762
ApaLI G'TGCA_C
Cuts at: 5819 6975 5819
Size: 1156 6606
ApoI r'AATT_y
Cuts at: 1493 2364 2391 3472 3494 4047 4483 5071 5297
Size: 871 27 1081 22 553 436 588 226
Cuts at: 5297 5449 7558 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1081 871 588 553 436 226
152 27 22
AseI AT'TA_AT
Cuts at: 160 1032 6316 160
Size: 872 5284 1606
Fragments arranged by size:
5284 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7762
AvaI C'yCGr_G
Cuts at: 1870 1943 5087 1870
Size: 73 3144 4545
Fragments arranged by size:
4545 3144 73
AvrII C'CTAG_G
Cuts at: 4442 4442
Size: 7762
BamHI G'GATC_C
Cuts at: 2683 5092 5459 2683
Size: 2409 367 4986
Fragments arranged by size:
4986 2409 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5083 6538 618
Size: 325 217 627 3296 1455 1842
Fragments arranged by size:
3296 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2152 4209 5081 1391
Size: 761 2057 872 4072
Fragments arranged by size:
4072 2057 872 761
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3080 3645 3680 3911 5036 928
Size: 971 1181 565 35 231 1125 3654
Fragments arranged by size:
3654 1181 1125 971 565 231 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5953 5987 1903
Size: 34 4016 34 3678
Fragments arranged by size:
4016 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5654 6864 5654
Size: 1210 6552
BclI T'GATC_A
Cuts at: 1548 2461 2482 3718 1548
Size: 913 21 1236 5592
Fragments arranged by size:
5592 1236 913 21
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2773 6373 136
Size: 107 122 71 631 605 1101 3600 1525
Fragments arranged by size:
3600 1525 1101 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4189 7757 4189
Size: 3568 4194
BlpI GC'TnA_GC
Cuts at: 4203 5179 4203
Size: 976 6786
Bme1580I G_kGCm'C
Cuts at: 1790 2152 2492 2778 5823 6979 1790
Size: 362 340 286 3045 1156 2573
Fragments arranged by size:
3045 2573 1156 362 340 286
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7762
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2470 3870 5493
Size: 416 53 83 399 24 1053 1400 1623
Cuts at: 5493 6451 442
Size: 958 1753
Fragments arranged by size:
1753 1623 1400 1053 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3201 3722 4707 6422 1239
Size: 1962 521 985 1715 2579
Fragments arranged by size:
2579 1962 1715 985 521
Bpu10I CC'TnA_GC
Cuts at: 2574 3267 4107 2574
Size: 693 840 6229
Fragments arranged by size:
6229 840 693
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4699 5887 6752 7050 4699
Size: 1188 865 298 5411
Fragments arranged by size:
5411 1188 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4169 6425 882
Size: 617 2670 2256 2219
Fragments arranged by size:
2670 2256 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5553 493
Size: 711 4349 2702
Fragments arranged by size:
4349 2702 711
BsaBI GATnn'nnATC
Cuts at: 7756 7756
Size: 7762
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5569 5951 6586 275
Size: 53 83 186 936 4036 382 635 1451
Fragments arranged by size:
4036 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6195 6593 6867 7014 7425 6195
Size: 398 274 147 411 6532
Fragments arranged by size:
6532 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2393 2423 1178
Size: 30 165 30 990 30 6517
Fragments arranged by size:
6517 990 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3539 4844 1186
Size: 2353 1305 4104
Fragments arranged by size:
4104 2353 1305
BseYI C'CCAG_C
Cuts at: 6965 7461 6965
Size: 496 7266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3548 1163
Size: 573 1812 5377
Fragments arranged by size:
5377 1812 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5212 5973 7001 1540
Size: 275 186 3211 761 1028 2301
Fragments arranged by size:
3211 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5823 5908 6979 5823
Size: 85 1071 6606
Fragments arranged by size:
6606 1071 85
BsmI GAATG_Cn'
Cuts at: 4462 5273 5366 4462
Size: 811 93 6858
Fragments arranged by size:
6858 811 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2492 2778 4209 5081 5823 5908
Size: 399 362 340 286 1431 872 742 85
Cuts at: 5908 6979 1391
Size: 1071 2174
Fragments arranged by size:
2174 1431 1071 872 742 399 362 340
286 85
BspEI T'CCGG_A
Cuts at: 7425 7425
Size: 7762
BspHI T'CATG_A
Cuts at: 2178 4054 4506 5651 7381 2178
Size: 1876 452 1145 1730 2559
Fragments arranged by size:
2559 1876 1730 1145 452
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4723 5117 844
Size: 3879 394 3489
Fragments arranged by size:
3879 3489 394
BsrBI CCG'CTC
Cuts at: 1394 1823 5649 1394
Size: 429 3826 3507
Fragments arranged by size:
3826 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3413 3651 3798 4535 6257 6431 65
Size: 1183 2165 238 147 737 1722 174 1396
Fragments arranged by size:
2165 1722 1396 1183 737 238 174 147
BsrFI r'CCGG_y
Cuts at: 1700 1810 6406 6593 1700
Size: 110 4596 187 2869
Fragments arranged by size:
4596 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3348 96
Size: 3252 4510
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7762
BssSI C'ACGA_G
Cuts at: 5822 6834 5822
Size: 1012 6750
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4537 794
Size: 3743 4019
BstBI TT'CG_AA
Cuts at: 6566 6566
Size: 7762
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7762
BstYI r'GATC_y
Cuts at: 1972 2683 4189 5092 5459 5844 5861 7313 7399
Size: 711 1506 903 367 385 17 1452 86
Cuts at: 7399 7411 7757 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1506 1452 903 711 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7023
Fragments arranged by size:
7023 683 56
BtsI GCAGTG_nn'
Cuts at: 2596 3450 5278 6072 6092 2596
Size: 854 1828 794 20 4266
Fragments arranged by size:
4266 1828 854 794 20
ClaI AT'CG_AT
Cuts at: 1554 2374 1554
Size: 820 6942
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7727
DraI TTT'AAA
Cuts at: 3841 3864 4482 4500 4593 4905 5066 3841
Size: 23 618 18 93 312 161 6537
Fragments arranged by size:
6537 618 312 161 93 23 18
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3152 6769 809
Size: 507 1836 3617 1802
Fragments arranged by size:
3617 1836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5209 6098 7727
Size: 54 1425 50 275 18 3379 889 1629
Cuts at: 7727 8
Size: 43
Fragments arranged by size:
3379 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5209 1537
Size: 275 3397 4090
Fragments arranged by size:
4090 3397 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2029 2601 2967 3960 5692 1353
Size: 676 572 366 993 1732 3423
Fragments arranged by size:
3423 1732 993 676 572 366
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6349 6723 6869 387
Size: 1064 138 4760 374 146 1280
Fragments arranged by size:
4760 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2223 2295 2574 2743 3092 3201 3722
Size: 393 591 72 279 169 349 109 521
Cuts at: 3722 4377 4707 5825 6422 7209 7477 1239
Size: 655 330 1118 597 787 268 1524
Fragments arranged by size:
1524 1118 787 655 597 591 521 393
349 330 279 268 169 109 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2761 4131 2761
Size: 1370 6392
EcoRI G'AATT_C
Cuts at: 5071 5449 5071
Size: 378 7384
EcoRV GAT'ATC
Cuts at: 2176 2176
Size: 7762
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2630 2794 2630
Size: 164 7598
FspI TGC'GCA
Cuts at: 4028 6268 4028
Size: 2240 5522
HaeII r_GCGC'y
Cuts at: 1678 6909 1678
Size: 5231 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3374 3406 6403 6435 6477
Size: 32 135 32 1802 32 2997 32 42
Cuts at: 6477 6509 1373
Size: 32 2626
Fragments arranged by size:
2997 2626 1802 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2693 2754 3145 4297 5106 5352
Size: 988 281 755 61 391 1152 809 246
Cuts at: 5352 669
Size: 3079
Fragments arranged by size:
3079 1152 988 809 755 391 281 246
61
HindIII A'AGCT_T
Cuts at: 748 3916 748
Size: 3168 4594
HpaI GTT'AAC
Cuts at: 5352 5352
Size: 7762
KpnI G_GTAC'C
Cuts at: 5087 5087
Size: 7762
MfeI C'AATT_G
Cuts at: 5361 5361
Size: 7762
MluI A'CGCG_T
Cuts at: 7638 7638
Size: 7762
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2095 3174 4594 6876 7060 1305
Size: 790 1079 1420 2282 184 2007
Fragments arranged by size:
2282 2007 1420 1079 790 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5940
Fragments arranged by size:
5940 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2781 4096 4833 5720 6079 6238 518
Size: 648 1615 1315 737 887 359 159 2042
Fragments arranged by size:
2042 1615 1315 887 737 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3035 4024 5178 5855 7003 7248 1606
Size: 1429 989 1154 677 1148 245 2120
Fragments arranged by size:
2120 1429 1154 1148 989 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7210
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7762
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7762
NotI GC'GGCC_GC
Cuts at: 5209 5209
Size: 7762
NspI r_CATG'y
Cuts at: 1132 1229 3143 3355 4477 5120 6665 1132
Size: 97 1914 212 1122 643 1545 2229
Fragments arranged by size:
2229 1914 1545 1122 643 212 97
PasI CC'CwG_GG
Cuts at: 1348 4522 1348
Size: 3174 4588
PciI A'CATG_T
Cuts at: 3351 6661 3351
Size: 3310 4452
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7762
PmeI GTTT'AAAC
Cuts at: 5066 5066
Size: 7762
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5831 5863 7344 7376 5831
Size: 32 1481 32 6217
Fragments arranged by size:
6217 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7762
PsiI TTA'TAA
Cuts at: 2834 2976 5332 2834
Size: 142 2356 5264
Fragments arranged by size:
5264 2356 142
PspOMI G'GGCC_C
Cuts at: 2148 2148
Size: 7762
PstI C_TGCA'G
Cuts at: 830 2409 3021 5114 830
Size: 1579 612 2093 3478
Fragments arranged by size:
3478 2093 1579 612
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7762
PvuII CAG'CTG
Cuts at: 3035 4024 3035
Size: 989 6773
SalI G'TCGA_C
Cuts at: 1936 3143 5104 1936
Size: 1207 1961 4594
Fragments arranged by size:
4594 1961 1207
SapI GCTCTTCn'nnn_
Cuts at: 2967 2967
Size: 7762
SbfI CC_TGCA'GG
Cuts at: 5114 5114
Size: 7762
ScaI AGT'ACT
Cuts at: 2304 3768 6010 7722 2304
Size: 1464 2242 1712 2344
Fragments arranged by size:
2344 2242 1712 1464
SfcI C'TryA_G
Cuts at: 826 1045 2405 3017 4858 5110 6245 6926 7117
Size: 219 1360 612 1841 252 1135 681 191
Cuts at: 7117 826
Size: 1471
Fragments arranged by size:
1841 1471 1360 1135 681 612 252 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7762
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7762
SmaI CCC'GGG
Cuts at: 1872 5089 1872
Size: 3217 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2615 2779 4678 5866 6767 7029
Size: 197 926 672 164 1899 1188 901 262
Cuts at: 7029 820
Size: 1553
Fragments arranged by size:
1899 1553 1188 926 901 672 262 197
164
SnaBI TAC'GTA
Cuts at: 493 5553 493
Size: 5060 2702
SpeI A'CTAG_T
Cuts at: 152 4082 152
Size: 3930 3832
SphI G_CATG'C
Cuts at: 1132 5120 1132
Size: 3988 3774
SspI AAT'ATT
Cuts at: 5 52 3277 3310 3655 3860 5686 5
Size: 47 3225 33 345 205 1826 2081
Fragments arranged by size:
3225 2081 1826 345 205 47 33
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7762
StyI C'CwwG_G
Cuts at: 513 1065 2769 4308 4442 5201 513
Size: 552 1704 1539 134 759 3074
Fragments arranged by size:
3074 1704 1539 759 552 134
SwaI ATTT'AAAT
Cuts at: 3864 3864
Size: 7762
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5789 5806 5959 6144 6589 325
Size: 1255 4209 17 153 185 445 1498
Fragments arranged by size:
4209 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2302 3348 3766
Size: 274 80 33 51 1443 325 1046 418
Cuts at: 3766 3808 3819 6008 7720 96
Size: 42 11 2189 1712 138
Fragments arranged by size:
2189 1712 1443 1046 418 325 274 138
80 51 42 33 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3188 3220 3878 3910 7722 7754 3188
Size: 32 658 32 3812 32 3196
Fragments arranged by size:
3812 3196 658 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3679 1316
Size: 2363 5399
XbaI T'CTAG_A
Cuts at: 2027 5098 2027
Size: 3071 4691
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2227 2227
Size: 7762
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7762
XmaI C'CCGG_G
Cuts at: 1870 5087 1870
Size: 3217 4545
XmnI GAAnn'nnTTC
Cuts at: 4100 5891 4100
Size: 1791 5971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5570 276
Size: 53 83 186 936 4036 2468
Fragments arranged by size:
4036 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI