Restriction map for
pFN21AE3341
(Circular) (Six-base) MAPSORT of: pFN21AE3341.seq Check: 6972 from: 1 to: 7663
pFN21AE3341 7663 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5473 278
Size: 53 83 186 936 3937 2468
Fragments arranged by size:
3937 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2079 5006 1937
Size: 142 2927 4594
Fragments arranged by size:
4594 2927 142
Acc65I G'GTAC_C
Cuts at: 2767 4984 2767
Size: 2217 5446
AclI AA'CG_TT
Cuts at: 1563 5790 6163 1563
Size: 4227 373 3063
Fragments arranged by size:
4227 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3559 5726 7110 7378 1632
Size: 1927 2167 1384 268 1917
Fragments arranged by size:
2167 1927 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7466
AflIII A'CryG_T
Cuts at: 1203 4632 6562 7539 1203
Size: 3429 1930 977 1327
Fragments arranged by size:
3429 1930 1327 977
AgeI A'CCGG_T
Cuts at: 4782 6494 4782
Size: 1712 5951
AhdI GACnn_n'nnGTC
Cuts at: 6392 6392
Size: 7663
AleI CACnn'nnGTG
Cuts at: 1166 2471 1166
Size: 1305 6358
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3692 1184
Size: 2508 5155
AlwNI CAG_nnn'CTG
Cuts at: 2666 3296 3560 4808 5406 6978 2666
Size: 630 264 1248 598 1572 3351
Fragments arranged by size:
3351 1572 1248 630 598 264
ApaLI G'TGCA_C
Cuts at: 2923 4835 5720 6876 2923
Size: 1912 885 1156 3710
Fragments arranged by size:
3710 1912 1156 885
ApoI r'AATT_y
Cuts at: 1493 2309 3896 4403 4613 4730 4972 5198 5350
Size: 816 1587 507 210 117 242 226 152
Cuts at: 5350 7459 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1587 816 507 242 226 210
152 117
AseI AT'TA_AT
Cuts at: 160 1032 6217 160
Size: 872 5185 1606
Fragments arranged by size:
5185 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7663
AvaI C'yCGr_G
Cuts at: 1870 1943 2274 3664 4308 4988 1870
Size: 73 331 1390 644 680 4545
Fragments arranged by size:
4545 1390 680 644 331 73
BamHI G'GATC_C
Cuts at: 4714 4993 5360 4714
Size: 279 367 7017
Fragments arranged by size:
7017 367 279
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2767 4984 6439 618
Size: 325 217 627 980 2217 1455 1842
Fragments arranged by size:
2217 1842 1455 980 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2287 3585 4691 4982 1391
Size: 896 1298 1106 291 4072
Fragments arranged by size:
4072 1298 1106 896 291
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2327 2667 3191 928
Size: 971 428 340 524 5400
Fragments arranged by size:
5400 971 524 428 340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5854 5888 1903
Size: 34 3917 34 3678
Fragments arranged by size:
3917 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5555 6765 5555
Size: 1210 6453
BclI T'GATC_A
Cuts at: 1548 4092 1548
Size: 2544 5119
BfrBI ATG'CAT
Cuts at: 4130 4130
Size: 7663
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6274 136
Size: 107 122 71 631 605 4602 1525
Fragments arranged by size:
4602 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2114 3945 7658 2114
Size: 1831 3713 2119
Fragments arranged by size:
3713 2119 1831
BlpI GC'TnA_GC
Cuts at: 5080 5080
Size: 7663
Bme1580I G_kGCm'C
Cuts at: 1790 2885 2927 4839 5724 6880 1790
Size: 1095 42 1912 885 1156 2573
Fragments arranged by size:
2573 1912 1156 1095 885 42
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7663
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2635 5394 6352
Size: 416 53 83 399 24 1218 2759 958
Cuts at: 6352 442
Size: 1753
Fragments arranged by size:
2759 1753 1218 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7663
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3072 4135 3072
Size: 1063 6600
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4675 4748 6323 1239
Size: 3436 73 1575 2579
Fragments arranged by size:
3436 2579 1575 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2338 4337 5788 6653 6951 2338
Size: 1999 1451 865 298 3050
Fragments arranged by size:
3050 1999 1451 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3661 6326 882
Size: 617 2162 2665 2219
Fragments arranged by size:
2665 2219 2162 617
BsaAI yAC'GTr
Cuts at: 493 1204 3541 3807 4840 5454 493
Size: 711 2337 266 1033 614 2702
Fragments arranged by size:
2702 2337 1033 711 614 266
BsaBI GATnn'nnATC
Cuts at: 4364 7657 4364
Size: 3293 4370
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2343 3919 5470 5852
Size: 53 83 186 936 810 1576 1551 382
Cuts at: 5852 6487 275
Size: 635 1451
Fragments arranged by size:
1576 1551 1451 936 810 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4327 4368 4782 6096 6494 6768 6915 7326 4327
Size: 41 414 1314 398 274 147 411 4664
Fragments arranged by size:
4664 1314 414 411 398 274 147 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3709 3739 1178
Size: 30 165 30 2306 30 5102
Fragments arranged by size:
5102 2306 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2819 4009 4612 1186
Size: 1633 1190 603 4237
Fragments arranged by size:
4237 1633 1190 603
BseYI C'CCAG_C
Cuts at: 2009 2237 2795 3125 6866 7362 2009
Size: 228 558 330 3741 496 2310
Fragments arranged by size:
3741 2310 558 496 330 228
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2962 1163
Size: 573 1226 5864
Fragments arranged by size:
5864 1226 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5113 5874 6902 1540
Size: 275 186 3112 761 1028 2301
Fragments arranged by size:
3112 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2927 4691 4839 5724 5809 6880 2927
Size: 1764 148 885 85 1071 3710
Fragments arranged by size:
3710 1764 1071 885 148 85
BsiWI C'GTAC_G
Cuts at: 3807 4461 3807
Size: 654 7009
BsmI GAATG_Cn'
Cuts at: 2825 4130 5174 5267 2825
Size: 1305 1044 93 5221
Fragments arranged by size:
5221 1305 1044 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2357 3712 3926 2357
Size: 1355 214 6094
Fragments arranged by size:
6094 1355 214
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2287 2885 2927 3585 4691 4839 4982
Size: 399 497 598 42 658 1106 148 143
Cuts at: 4982 5724 5809 6880 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1106 1071 742 658 598 497 399
148 143 85 42
BspEI T'CCGG_A
Cuts at: 4327 7326 4327
Size: 2999 4664
BspHI T'CATG_A
Cuts at: 2493 5552 7282 2493
Size: 3059 1730 2874
Fragments arranged by size:
3059 2874 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5018 844
Size: 4174 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2809 2814 5550 1394
Size: 429 986 5 2736 3507
Fragments arranged by size:
3507 2736 986 429 5
BsrDI GCAATG_nn'
Cuts at: 65 1248 2230 2445 3333 3693 4554 4923 6158
Size: 1183 982 215 888 360 861 369 1235
Cuts at: 6158 6332 65
Size: 174 1396
Fragments arranged by size:
1396 1235 1183 982 888 861 369 360
215 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4782 6307 6494 1700
Size: 110 2972 1525 187 2869
Fragments arranged by size:
2972 2869 1525 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7663
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7663
BssSI C'ACGA_G
Cuts at: 2920 5723 6735 2920
Size: 2803 1012 3848
Fragments arranged by size:
3848 2803 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4939 794
Size: 4145 3518
BstBI TT'CG_AA
Cuts at: 6467 6467
Size: 7663
BstEII G'GTnAC_C
Cuts at: 1184 3119 4530 1184
Size: 1935 1411 4317
Fragments arranged by size:
4317 1935 1411
BstXI CCAn_nnnn'nTGG
Cuts at: 3340 4024 4777 3340
Size: 684 753 6226
Fragments arranged by size:
6226 753 684
BstYI r'GATC_y
Cuts at: 1972 2114 2511 3814 3945 4714 4993 5360 5745
Size: 142 397 1303 131 769 279 367 385
Cuts at: 5745 5762 7214 7300 7312 7658 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1303 769 397 385 367 346
279 142 131 86 17 12
BstZ17I GTA'TAC
Cuts at: 2080 2080
Size: 7663
Bsu36I CC'TnA_GG
Cuts at: 4136 4136
Size: 7663
BtgI C'CryG_G
Cuts at: 513 1065 2004 2859 513
Size: 552 939 855 5317
Fragments arranged by size:
5317 939 855 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2563 487
Size: 683 56 1337 5587
Fragments arranged by size:
5587 1337 683 56
BtsI GCAGTG_nn'
Cuts at: 3676 3834 3919 4701 4912 5179 5973 5993 3676
Size: 158 85 782 211 267 794 20 5346
Fragments arranged by size:
5346 794 782 267 211 158 85 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7663
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3091 3126 529
Size: 35 2527 35 5066
Fragments arranged by size:
5066 2527 35 35
DraI TTT'AAA
Cuts at: 4967 4967
Size: 7663
DraIII CAC_nnn'GTG
Cuts at: 2922 2922
Size: 7663
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6670 809
Size: 507 5354 1802
Fragments arranged by size:
5354 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3070 4245 5110
Size: 54 1425 50 275 18 1240 1175 865
Cuts at: 5110 5999 7628 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1240 1175 889 865 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5110 1537
Size: 275 3298 4090
Fragments arranged by size:
4090 3298 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2048 2712 3753 4664 5593 1353
Size: 695 664 1041 911 929 3423
Fragments arranged by size:
3423 1041 929 911 695 664
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6250 6624 6770 387
Size: 1064 138 4661 374 146 1280
Fragments arranged by size:
4661 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4689 4689
Size: 7663
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3559 4675 4748 5726 6323 7110 7378
Size: 393 1927 1116 73 978 597 787 268
Cuts at: 7378 1239
Size: 1524
Fragments arranged by size:
1927 1524 1116 978 787 597 393 268
73
EcoNI CCTnn'n_nnAGG
Cuts at: 2154 4010 2154
Size: 1856 5807
EcoO109I rG'GnC_Cy
Cuts at: 2571 4473 4852 2571
Size: 1902 379 5382
Fragments arranged by size:
5382 1902 379
EcoRI G'AATT_C
Cuts at: 4730 4972 5350 4730
Size: 242 378 7043
Fragments arranged by size:
7043 378 242
EcoRV GAT'ATC
Cuts at: 3502 3502
Size: 7663
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4813 4813
Size: 7663
FspI TGC'GCA
Cuts at: 6169 6169
Size: 7663
HaeII r_GCGC'y
Cuts at: 1678 6810 1678
Size: 5132 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2099 2131 2951 2983 4146
Size: 32 135 32 527 32 820 32 1163
Cuts at: 4146 4178 4706 4738 6304 6336 6378 6410 1373
Size: 32 528 32 1566 32 42 32 2626
Fragments arranged by size:
2626 1566 1163 820 528 527 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2707 3022 4630 5007 5253 669
Size: 988 281 769 315 1608 377 246 3079
Fragments arranged by size:
3079 1608 988 769 377 315 281 246
HindIII A'AGCT_T
Cuts at: 748 2826 748
Size: 2078 5585
HpaI GTT'AAC
Cuts at: 5253 5253
Size: 7663
KpnI G_GTAC'C
Cuts at: 2771 4988 2771
Size: 2217 5446
MfeI C'AATT_G
Cuts at: 4389 5262 4389
Size: 873 6790
MluI A'CGCG_T
Cuts at: 7539 7539
Size: 7663
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4617 4692 6777 6961 1305
Size: 3312 75 2085 184 2007
Fragments arranged by size:
3312 2085 2007 184 75
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5841
Fragments arranged by size:
5841 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2471 4262 4775 5621 5980 6139 518
Size: 648 1305 1791 513 846 359 159 2042
Fragments arranged by size:
2042 1791 1305 846 648 513 359 159
MspA1I CmG'CkG
Cuts at: 1606 3485 3560 4171 4570 4805 5079 5756 6904
Size: 1879 75 611 399 235 274 677 1148
Cuts at: 6904 7149 1606
Size: 245 2120
Fragments arranged by size:
2120 1879 1148 677 611 399 274 245
235 75
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6172
Fragments arranged by size:
6172 939 552
NdeI CA'TA_TG
Cuts at: 387 3790 387
Size: 3403 4260
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7663
NotI GC'GGCC_GC
Cuts at: 5110 5110
Size: 7663
NsiI A_TGCA'T
Cuts at: 4132 4132
Size: 7663
NspI r_CATG'y
Cuts at: 1132 1229 4183 4306 4636 5021 6566 1132
Size: 97 2954 123 330 385 1545 2229
Fragments arranged by size:
2954 2229 1545 385 330 123 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7663
PciI A'CATG_T
Cuts at: 4632 6562 4632
Size: 1930 5733
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7663
PfoI T'CCnGG_A
Cuts at: 2514 3417 3614 2514
Size: 903 197 6563
Fragments arranged by size:
6563 903 197
PmeI GTTT'AAAC
Cuts at: 4967 4967
Size: 7663
PmlI CAC'GTG
Cuts at: 3541 4840 3541
Size: 1299 6364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5732 5764 7245 7277 5732
Size: 32 1481 32 6118
Fragments arranged by size:
6118 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4852 4852
Size: 7663
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7663
PsiI TTA'TAA
Cuts at: 5233 5233
Size: 7663
PstI C_TGCA'G
Cuts at: 830 5015 830
Size: 4185 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7663
PvuII CAG'CTG
Cuts at: 3485 3560 4171 4570 4805 3485
Size: 75 611 399 235 6343
Fragments arranged by size:
6343 611 399 235 75
SacI G_AGCT'C
Cuts at: 4691 4691
Size: 7663
SalI G'TCGA_C
Cuts at: 1936 5005 1936
Size: 3069 4594
SbfI CC_TGCA'GG
Cuts at: 5015 5015
Size: 7663
ScaI AGT'ACT
Cuts at: 5911 7623 5911
Size: 1712 5951
SfcI C'TryA_G
Cuts at: 826 1045 5011 6146 6827 7018 826
Size: 219 3966 1135 681 191 1471
Fragments arranged by size:
3966 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7663
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7663
SmaI CCC'GGG
Cuts at: 1872 4990 1872
Size: 3118 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2317 4316 5767 6668 6930 820
Size: 197 926 374 1999 1451 901 262 1553
Fragments arranged by size:
1999 1553 1451 926 901 374 262 197
SnaBI TAC'GTA
Cuts at: 493 3807 5454 493
Size: 3314 1647 2702
Fragments arranged by size:
3314 2702 1647
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7663
SphI G_CATG'C
Cuts at: 1132 4183 5021 1132
Size: 3051 838 3774
Fragments arranged by size:
3774 3051 838
SspI AAT'ATT
Cuts at: 5 52 5587 5
Size: 47 5535 2081
Fragments arranged by size:
5535 2081 47
StuI AGG'CCT
Cuts at: 1517 3510 4609 1517
Size: 1993 1099 4571
Fragments arranged by size:
4571 1993 1099
StyI C'CwwG_G
Cuts at: 513 1065 2004 3585 4122 5102 513
Size: 552 939 1581 537 980 3074
Fragments arranged by size:
3074 1581 980 939 552 537
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2124 2483 2949 5690 5707 5860 6045
Size: 1255 544 359 466 2741 17 153 185
Cuts at: 6045 6490 325
Size: 445 1498
Fragments arranged by size:
2741 1498 1255 544 466 445 359 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2376 2739 4104
Size: 274 80 33 51 1443 399 363 1365
Cuts at: 4104 5909 7621 96
Size: 1805 1712 138
Fragments arranged by size:
1805 1712 1443 1365 399 363 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2147 2179 2610 2642 4242 4274 7623 7655 2147
Size: 32 431 32 1600 32 3349 32 2155
Fragments arranged by size:
3349 2155 1600 431 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2060 1316
Size: 744 6919
XbaI T'CTAG_A
Cuts at: 4999 4999
Size: 7663
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2477 3649 2477
Size: 1172 6491
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7663
XmaI C'CCGG_G
Cuts at: 1870 4988 1870
Size: 3118 4545
XmnI GAAnn'nnTTC
Cuts at: 5792 5792
Size: 7663
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5471 276
Size: 53 83 186 936 3937 2468
Fragments arranged by size:
3937 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI