Restriction map for
pFN21AE3339
(Circular) (Six-base) MAPSORT of: pFN21AE3339.seq Check: 1572 from: 1 to: 7633
pFN21AE3339 7633 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3217 3217
Size: 7633
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5443 278
Size: 53 83 186 936 3907 2468
Fragments arranged by size:
3907 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4976 1937
Size: 3039 4594
Acc65I G'GTAC_C
Cuts at: 3480 4530 4954 3480
Size: 1050 424 6159
Fragments arranged by size:
6159 1050 424
AclI AA'CG_TT
Cuts at: 1563 5760 6133 1563
Size: 4197 373 3063
Fragments arranged by size:
4197 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3812 4410 4616 5696 7080 7348 1632
Size: 2180 598 206 1080 1384 268 1917
Fragments arranged by size:
2180 1917 1384 1080 598 268 206
AfeI AGC'GCT
Cuts at: 2513 2612 2513
Size: 99 7534
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7436
AflIII A'CryG_T
Cuts at: 1203 2196 6532 7509 1203
Size: 993 4336 977 1327
Fragments arranged by size:
4336 1327 993 977
AgeI A'CCGG_T
Cuts at: 4533 6464 4533
Size: 1931 5702
AhdI GACnn_n'nnGTC
Cuts at: 2482 6362 2482
Size: 3880 3753
AleI CACnn'nnGTG
Cuts at: 1166 3764 1166
Size: 2598 5035
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2925 4671 1184
Size: 1741 1746 4146
Fragments arranged by size:
4146 1746 1741
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2288 2320 2288
Size: 32 7601
AlwNI CAG_nnn'CTG
Cuts at: 2176 4388 5376 6948 2176
Size: 2212 988 1572 2861
Fragments arranged by size:
2861 2212 1572 988
ApaI G_GGCC'C
Cuts at: 2978 3150 3193 2978
Size: 172 43 7418
Fragments arranged by size:
7418 172 43
ApaLI G'TGCA_C
Cuts at: 2733 3173 5690 6846 2733
Size: 440 2517 1156 3520
Fragments arranged by size:
3520 2517 1156 440
ApoI r'AATT_y
Cuts at: 1493 4112 4942 5168 5320 7429 1493
Size: 2619 830 226 152 2109 1697
Fragments arranged by size:
2619 2109 1697 830 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6187 160
Size: 872 5155 1606
Fragments arranged by size:
5155 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7633
AvaI C'yCGr_G
Cuts at: 1870 1943 2065 2566 2966 3192 3852 4769 4886
Size: 73 122 501 400 226 660 917 117
Cuts at: 4886 4958 1870
Size: 72 4545
Fragments arranged by size:
4545 917 660 501 400 226 122 117
73 72
BamHI G'GATC_C
Cuts at: 2137 4740 4963 5330 2137
Size: 2603 223 367 4440
Fragments arranged by size:
4440 2603 367 223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2570 2773 2787 3480 4530
Size: 325 217 627 783 203 14 693 1050
Cuts at: 4530 4890 4954 6409 618
Size: 360 64 1455 1842
Fragments arranged by size:
1842 1455 1050 783 693 627 360 325
217 203 64 14
BanII G_rGCy'C
Cuts at: 1391 2965 2978 3150 3193 3201 3724 4132 4578
Size: 1574 13 172 43 8 523 408 446
Cuts at: 4578 4952 1391
Size: 374 4072
Fragments arranged by size:
4072 1574 523 446 408 374 172 43
13 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2593 928
Size: 971 694 5968
Fragments arranged by size:
5968 971 694
BbvCI CC'TCA_GC
Cuts at: 4854 4854
Size: 7633
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3809 3843 5824 5858 1903
Size: 34 1872 34 1981 34 3678
Fragments arranged by size:
3678 1981 1872 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5525 6735 5525
Size: 1210 6423
BclI T'GATC_A
Cuts at: 1548 3592 4101 1548
Size: 2044 509 5080
Fragments arranged by size:
5080 2044 509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2026 2579 2760
Size: 107 122 71 631 605 354 553 181
Cuts at: 2760 2850 4728 6244 136
Size: 90 1878 1516 1525
Fragments arranged by size:
1878 1525 1516 631 605 553 354 181
122 107 90 71
BglII A'GATC_T
Cuts at: 7628 7628
Size: 7633
BlpI GC'TnA_GC
Cuts at: 4463 5050 4463
Size: 587 7046
Bme1580I G_kGCm'C
Cuts at: 1790 2737 2897 2978 3131 3150 3177 3193 4609
Size: 947 160 81 153 19 27 16 1416
Cuts at: 4609 4893 5694 6850 1790
Size: 284 801 1156 2573
Fragments arranged by size:
2573 1416 1156 947 801 284 160 153
81 27 19 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7633
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3112 3578 5364
Size: 416 53 83 399 24 1695 466 1786
Cuts at: 5364 6322 442
Size: 958 1753
Fragments arranged by size:
1786 1753 1695 958 466 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7633
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 7633
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3657 3794 4664 4862 6293 1239
Size: 2418 137 870 198 1431 2579
Fragments arranged by size:
2579 2418 1431 870 198 137
Bpu10I CC'TnA_GC
Cuts at: 3020 4854 3020
Size: 1834 5799
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5758 6623 6921 5758
Size: 865 298 6470
Fragments arranged by size:
6470 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2357 2469 2615 6296 882
Size: 617 858 112 146 3681 2219
Fragments arranged by size:
3681 2219 858 617 146 112
BsaAI yAC'GTr
Cuts at: 493 1204 2293 2892 3457 5424 493
Size: 711 1089 599 565 1967 2702
Fragments arranged by size:
2702 1967 1089 711 599 565
BsaBI GATnn'nnATC
Cuts at: 3768 4100 4370 4874 7627 3768
Size: 332 270 504 2753 3774
Fragments arranged by size:
3774 2753 504 332 270
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3085 4074 5440 5822
Size: 53 83 186 936 1552 989 1366 382
Cuts at: 5822 6457 275
Size: 635 1451
Fragments arranged by size:
1552 1451 1366 989 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2859 3708 4533 6066 6464 6738 6885 7296 2859
Size: 849 825 1533 398 274 147 411 3196
Fragments arranged by size:
3196 1533 849 825 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2288 2318 3633 3663 4652
Size: 30 165 30 885 30 1315 30 989
Cuts at: 4652 4682 1178
Size: 30 4129
Fragments arranged by size:
4129 1315 989 885 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 3654 1186
Size: 1102 1366 5165
Fragments arranged by size:
5165 1366 1102
BseYI C'CCAG_C
Cuts at: 2122 2669 2898 3067 3286 3723 3941 4590 4718
Size: 547 229 169 219 437 218 649 128
Cuts at: 4718 4814 6836 7332 2122
Size: 96 2022 496 2423
Fragments arranged by size:
2423 2022 649 547 496 437 229 219
218 169 128 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3437 3699 3813 4784 1163
Size: 573 1701 262 114 971 4012
Fragments arranged by size:
4012 1701 971 573 262 114
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5083 5844 6872 1540
Size: 275 186 3082 761 1028 2301
Fragments arranged by size:
3082 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2737 2909 2965 3177 4578 5694 5779 6850 2737
Size: 172 56 212 1401 1116 85 1071 3520
Fragments arranged by size:
3520 1401 1116 1071 212 172 85 56
BsmI GAATG_Cn'
Cuts at: 2406 3561 4902 5144 5237 2406
Size: 1155 1341 242 93 4802
Fragments arranged by size:
4802 1341 1155 242 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4762 4762
Size: 7633
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2737 2897 2909 2965 2978 3131 3150
Size: 399 947 160 12 56 13 153 19
Cuts at: 3150 3177 3193 3201 3724 4132 4578 4609 4893
Size: 27 16 8 523 408 446 31 284
Cuts at: 4893 4952 5694 5779 6850 1391
Size: 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 947 742 523 446 408 399
284 160 153 85 59 56 31 27
19 16 13 12 8
BspEI T'CCGG_A
Cuts at: 7296 7296
Size: 7633
BspHI T'CATG_A
Cuts at: 3595 4098 4578 5522 7252 3595
Size: 503 480 944 1730 3976
Fragments arranged by size:
3976 1730 944 503 480
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2702 3217 4988 844
Size: 1858 515 1771 3489
Fragments arranged by size:
3489 1858 1771 515
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 2949 4153 5520 1394
Size: 429 188 938 1204 1367 3507
Fragments arranged by size:
3507 1367 1204 938 429 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 4395 4680 6128 6302 65
Size: 1183 3147 285 1448 174 1396
Fragments arranged by size:
3147 1448 1396 1183 285 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4533 4611 6277 6464 1700
Size: 110 2723 78 1666 187 2869
Fragments arranged by size:
2869 2723 1666 187 110 78
BsrGI T'GTAC_A
Cuts at: 96 2193 2379 96
Size: 2097 186 5350
Fragments arranged by size:
5350 2097 186
BssHII G'CGCG_C
Cuts at: 1925 2072 2074 1925
Size: 147 2 7484
Fragments arranged by size:
7484 147 2
BssSI C'ACGA_G
Cuts at: 5693 6705 5693
Size: 1012 6621
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2748 2837 4060 4908 794
Size: 1954 89 1223 848 3519
Fragments arranged by size:
3519 1954 1223 848 89
BstBI TT'CG_AA
Cuts at: 6437 6437
Size: 7633
BstEII G'GTnAC_C
Cuts at: 1184 2437 1184
Size: 1253 6380
BstXI CCAn_nnnn'nTGG
Cuts at: 3161 3766 4814 3161
Size: 605 1048 5980
Fragments arranged by size:
5980 1048 605
BstYI r'GATC_y
Cuts at: 1972 2137 2259 4365 4407 4740 4963 5330 5715
Size: 165 122 2106 42 333 223 367 385
Cuts at: 5715 5732 7184 7270 7282 7628 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2106 1977 1452 385 367 346 333 223
165 122 86 42 17 12
Bsu36I CC'TnA_GG
Cuts at: 2728 3034 3932 4145 2728
Size: 306 898 213 6216
Fragments arranged by size:
6216 898 306 213
BtgI C'CryG_G
Cuts at: 513 1065 2004 2266 2442 2539 4827 513
Size: 552 939 262 176 97 2288 3319
Fragments arranged by size:
3319 2288 939 552 262 176 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4440 487
Size: 683 56 3214 3680
Fragments arranged by size:
3680 3214 683 56
BtsI GCAGTG_nn'
Cuts at: 2746 2827 2907 3219 4880 5149 5943 5963 2746
Size: 81 80 312 1661 269 794 20 4416
Fragments arranged by size:
4416 1661 794 312 269 81 80 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7633
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7598
DraI TTT'AAA
Cuts at: 4937 4937
Size: 7633
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4166 6640 809
Size: 507 2850 2474 1802
Fragments arranged by size:
2850 2474 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2950 3298 3963
Size: 54 1425 50 275 18 1120 348 665
Cuts at: 3963 3997 4185 4613 5080 5969 7598 8
Size: 34 188 428 467 889 1629 43
Fragments arranged by size:
1629 1425 1120 889 665 467 428 348
275 188 54 50 43 34 18
EagI C'GGCC_G
Cuts at: 1537 1812 5080 1537
Size: 275 3268 4090
Fragments arranged by size:
4090 3268 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4508 4713 5563 1353
Size: 3155 205 850 3423
Fragments arranged by size:
3423 3155 850 205
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6220 6594 6740 387
Size: 1064 138 4631 374 146 1280
Fragments arranged by size:
4631 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2963 4576 2963
Size: 1613 6020
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3657 3794 3812 4410 4616 4664 4862
Size: 393 2025 137 18 598 206 48 198
Cuts at: 4862 5696 6293 7080 7348 1239
Size: 834 597 787 268 1524
Fragments arranged by size:
2025 1524 834 787 598 597 393 268
206 198 137 48 18
EcoNI CCTnn'n_nnAGG
Cuts at: 4019 4144 4019
Size: 125 7508
EcoO109I rG'GnC_Cy
Cuts at: 2691 2724 2974 2975 2996 3146 3147 3189 3411
Size: 33 250 1 21 150 1 42 222
Cuts at: 3411 3572 3628 3823 3927 4249 4858 2691
Size: 161 56 195 104 322 609 5466
Fragments arranged by size:
5466 609 322 250 222 195 161 150
104 56 42 33 21 1 1
EcoRI G'AATT_C
Cuts at: 4942 5320 4942
Size: 378 7255
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 7633
FspI TGC'GCA
Cuts at: 6139 6139
Size: 7633
HaeII r_GCGC'y
Cuts at: 1678 2017 2515 2614 6780 1678
Size: 339 498 99 4166 2531
Fragments arranged by size:
4166 2531 498 339 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 3332 3364 4350
Size: 32 135 32 896 32 832 32 986
Cuts at: 4350 4382 4483 4515 4732 4764 6274 6306 6348
Size: 32 101 32 217 32 1510 32 42
Cuts at: 6348 6380 1373
Size: 32 2626
Fragments arranged by size:
2626 1510 986 896 832 217 135 101
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4977 5223 669
Size: 988 281 3039 246 3079
Fragments arranged by size:
3079 3039 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4628 748
Size: 3880 3753
HpaI GTT'AAC
Cuts at: 5223 5223
Size: 7633
KpnI G_GTAC'C
Cuts at: 3484 4534 4958 3484
Size: 1050 424 6159
Fragments arranged by size:
6159 1050 424
MfeI C'AATT_G
Cuts at: 2254 5232 2254
Size: 2978 4655
MluI A'CGCG_T
Cuts at: 7509 7509
Size: 7633
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2440 4358 6747 6931 1305
Size: 1135 1918 2389 184 2007
Fragments arranged by size:
2389 2007 1918 1135 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3300 3965 3999 4187 10
Size: 54 1425 343 1468 665 34 188 3456
Fragments arranged by size:
3456 1468 1425 665 343 188 54 34
MslI CAynn'nnrTG
Cuts at: 518 1166 3222 3326 3764 4236 4271 4781 5591
Size: 648 2056 104 438 472 35 510 810
Cuts at: 5591 5950 6109 518
Size: 359 159 2042
Fragments arranged by size:
2056 2042 810 648 510 472 438 359
159 104 35
MspA1I CmG'CkG
Cuts at: 1606 2059 2268 2669 3727 4165 4853 5049 5726
Size: 453 209 401 1058 438 688 196 677
Cuts at: 5726 6874 7119 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1058 688 677 453 438 401
245 209 196
NaeI GCC'GGC
Cuts at: 4613 4613
Size: 7633
NcoI C'CATG_G
Cuts at: 513 1065 2004 4827 513
Size: 552 939 2823 3319
Fragments arranged by size:
3319 2823 939 552
NdeI CA'TA_TG
Cuts at: 387 4597 387
Size: 4210 3423
NgoMIV G'CCGG_C
Cuts at: 4611 4611
Size: 7633
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7633
NotI GC'GGCC_GC
Cuts at: 5080 5080
Size: 7633
NspI r_CATG'y
Cuts at: 1132 1229 2200 2746 2835 2991 3221 3338 4991
Size: 97 971 546 89 156 230 117 1653
Cuts at: 4991 6536 1132
Size: 1545 2229
Fragments arranged by size:
2229 1653 1545 971 546 230 156 117
97 89
PasI CC'CwG_GG
Cuts at: 1348 2023 2333 3428 3501 4172 4354 4698 1348
Size: 675 310 1095 73 671 182 344 4283
Fragments arranged by size:
4283 1095 675 671 344 310 182 73
PciI A'CATG_T
Cuts at: 2196 6532 2196
Size: 4336 3297
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2150 2401 1108
Size: 1042 251 6340
Fragments arranged by size:
6340 1042 251
PfoI T'CCnGG_A
Cuts at: 3713 4323 3713
Size: 610 7023
PmeI GTTT'AAAC
Cuts at: 4937 4937
Size: 7633
PmlI CAC'GTG
Cuts at: 2293 2892 3457 2293
Size: 599 565 6469
Fragments arranged by size:
6469 599 565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2288 2320 5702 5734 7215 7247 2288
Size: 32 3382 32 1481 32 2674
Fragments arranged by size:
3382 2674 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2691 2996 3411 3572 3823 3927 4249 4858 2691
Size: 305 415 161 251 104 322 609 5466
Fragments arranged by size:
5466 609 415 322 305 251 161 104
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7633
PsiI TTA'TAA
Cuts at: 5203 5203
Size: 7633
PspOMI G'GGCC_C
Cuts at: 2974 3146 3189 2974
Size: 172 43 7418
Fragments arranged by size:
7418 172 43
PspXI vC'TCGA_Gb
Cuts at: 4769 4769
Size: 7633
PstI C_TGCA'G
Cuts at: 830 2304 2455 2753 3214 3525 4197 4985 830
Size: 1474 151 298 461 311 672 788 3478
Fragments arranged by size:
3478 1474 788 672 461 311 298 151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7633
PvuII CAG'CTG
Cuts at: 4165 4165
Size: 7633
SacI G_AGCT'C
Cuts at: 2965 4578 2965
Size: 1613 6020
SacII CC_GC'GG
Cuts at: 2269 2269
Size: 7633
SalI G'TCGA_C
Cuts at: 1936 4975 1936
Size: 3039 4594
SanDI GG'GwC_CC
Cuts at: 3411 3927 3411
Size: 516 7117
SapI GCTCTTCn'nnn_
Cuts at: 4508 4508
Size: 7633
SbfI CC_TGCA'GG
Cuts at: 4985 4985
Size: 7633
ScaI AGT'ACT
Cuts at: 5881 7593 5881
Size: 1712 5921
SfcI C'TryA_G
Cuts at: 826 1045 2300 2451 2749 2778 3210 3521 4193
Size: 219 1255 151 298 29 432 311 672
Cuts at: 4193 4981 6116 6797 6988 826
Size: 788 1135 681 191 1471
Fragments arranged by size:
1471 1255 1135 788 681 672 432 311
298 219 191 151 29
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7633
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7633
SmaI CCC'GGG
Cuts at: 1872 2067 2568 2968 3194 3854 4888 4960 1872
Size: 195 501 400 226 660 1034 72 4545
Fragments arranged by size:
4545 1034 660 501 400 226 195 72
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4769 5737 6638 6900 820
Size: 197 926 2826 968 901 262 1553
Fragments arranged by size:
2826 1553 968 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5424 493
Size: 4931 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7633
SphI G_CATG'C
Cuts at: 1132 2746 2991 3338 4991 1132
Size: 1614 245 347 1653 3774
Fragments arranged by size:
3774 1653 1614 347 245
SrfI GCCC'GGGC
Cuts at: 4888 4888
Size: 7633
SspI AAT'ATT
Cuts at: 5 52 5557 5
Size: 47 5505 2081
Fragments arranged by size:
5505 2081 47
StuI AGG'CCT
Cuts at: 1517 3354 1517
Size: 1837 5796
StyI C'CwwG_G
Cuts at: 513 1065 2004 2079 3840 4827 5072 513
Size: 552 939 75 1761 987 245 3074
Fragments arranged by size:
3074 1761 987 939 552 245 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2142 5660 5677 5830 6015 6460 325
Size: 1255 562 3518 17 153 185 445 1498
Fragments arranged by size:
3518 1498 1255 562 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2193 2379 5879
Size: 274 80 33 51 1443 216 186 3500
Cuts at: 5879 7591 96
Size: 1712 138
Fragments arranged by size:
3500 1712 1443 274 216 186 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3770 3802 7593 7625 3770
Size: 32 3791 32 3778
Fragments arranged by size:
3791 3778 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3887 1316
Size: 2571 5062
XbaI T'CTAG_A
Cuts at: 3493 4969 3493
Size: 1476 6157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2151 2798 3999 4169 4267 2151
Size: 647 1201 170 98 5517
Fragments arranged by size:
5517 1201 647 170 98
XhoI C'TCGA_G
Cuts at: 1943 4769 1943
Size: 2826 4807
XmaI C'CCGG_G
Cuts at: 1870 2065 2566 2966 3192 3852 4886 4958 1870
Size: 195 501 400 226 660 1034 72 4545
Fragments arranged by size:
4545 1034 660 501 400 226 195 72
XmnI GAAnn'nnTTC
Cuts at: 5762 5762
Size: 7633
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5441 276
Size: 53 83 186 936 3907 2468
Fragments arranged by size:
3907 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PsrI RsrII SexAI SfiI SfoI SwaI