Restriction map for
pFN21AE3333
(Circular) (Six-base) MAPSORT of: pFN21AE3333.seq Check: 645 from: 1 to: 7606
pFN21AE3333 7606 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2470 3011 2470
Size: 541 7065
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5416 278
Size: 53 83 186 936 3880 2468
Fragments arranged by size:
3880 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2822 3967 4949 1937
Size: 885 1145 982 4594
Fragments arranged by size:
4594 1145 982 885
Acc65I G'GTAC_C
Cuts at: 4927 4927
Size: 7606
AclI AA'CG_TT
Cuts at: 1563 4771 5733 6106 1563
Size: 3208 962 373 3063
Fragments arranged by size:
3208 3063 962 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2131 2246 3080 3365 3659 3888 5669 7053
Size: 499 115 834 285 294 229 1781 1384
Cuts at: 7053 7321 1632
Size: 268 1917
Fragments arranged by size:
1917 1781 1384 834 499 294 285 268
229 115
AfeI AGC'GCT
Cuts at: 3564 3564
Size: 7606
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7409
AflIII A'CryG_T
Cuts at: 1203 6505 7482 1203
Size: 5302 977 1327
Fragments arranged by size:
5302 1327 977
AgeI A'CCGG_T
Cuts at: 4565 6437 4565
Size: 1872 5734
AhdI GACnn_n'nnGTC
Cuts at: 2402 2874 6335 2402
Size: 472 3461 3673
Fragments arranged by size:
3673 3461 472
AleI CACnn'nnGTG
Cuts at: 1166 4566 1166
Size: 3400 4206
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2432 1184
Size: 1248 6358
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2786 2818 2786
Size: 32 7574
AlwNI CAG_nnn'CTG
Cuts at: 5349 6921 5349
Size: 1572 6034
ApaLI G'TGCA_C
Cuts at: 2417 3497 5663 6819 2417
Size: 1080 2166 1156 3204
Fragments arranged by size:
3204 2166 1156 1080
ApoI r'AATT_y
Cuts at: 1493 3437 4453 4889 4915 5141 5293 7402 1493
Size: 1944 1016 436 26 226 152 2109 1697
Fragments arranged by size:
2109 1944 1697 1016 436 226 152 26
AseI AT'TA_AT
Cuts at: 160 1032 4061 6160 160
Size: 872 3029 2099 1606
Fragments arranged by size:
3029 2099 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7606
AvaI C'yCGr_G
Cuts at: 1870 1943 2297 2508 3759 4931 1870
Size: 73 354 211 1251 1172 4545
Fragments arranged by size:
4545 1251 1172 354 211 73
BamHI G'GATC_C
Cuts at: 3847 4015 4936 5303 3847
Size: 168 921 367 6150
Fragments arranged by size:
6150 921 367 168
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3464 4460 4927 6382 618
Size: 325 217 627 1677 996 467 1455 1842
Fragments arranged by size:
1842 1677 1455 996 627 467 325 217
BanII G_rGCy'C
Cuts at: 1391 2103 3980 4535 4925 1391
Size: 712 1877 555 390 4072
Fragments arranged by size:
4072 1877 712 555 390
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2395 3903 4144 928
Size: 971 496 1508 241 4390
Fragments arranged by size:
4390 1508 971 496 241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5797 5831 1903
Size: 34 3860 34 3678
Fragments arranged by size:
3860 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2912 5498 6708 2912
Size: 2586 1210 3810
Fragments arranged by size:
3810 2586 1210
BclI T'GATC_A
Cuts at: 1548 4374 1548
Size: 2826 4780
BfrBI ATG'CAT
Cuts at: 4128 4128
Size: 7606
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6217 136
Size: 107 122 71 631 605 4545 1525
Fragments arranged by size:
4545 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7601 7601
Size: 7606
BlpI GC'TnA_GC
Cuts at: 5023 5023
Size: 7606
Bme1580I G_kGCm'C
Cuts at: 1790 2320 2421 3469 3501 4465 4759 5667 6823
Size: 530 101 1048 32 964 294 908 1156
Cuts at: 6823 1790
Size: 2573
Fragments arranged by size:
2573 1156 1048 964 908 530 294 101
32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7606
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3511 4659 4753
Size: 416 53 83 399 24 2094 1148 94
Cuts at: 4753 5337 6295 442
Size: 584 958 1753
Fragments arranged by size:
2094 1753 1148 958 584 416 399 94
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7606
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 7606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 3188 3945 4755 6266 1239
Size: 824 1125 757 810 1511 2579
Fragments arranged by size:
2579 1511 1125 824 810 757
Bpu10I CC'TnA_GC
Cuts at: 3366 3366
Size: 7606
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2279 5731 6596 6894 2279
Size: 3452 865 298 2991
Fragments arranged by size:
3452 2991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2123 2480 2806 3866 4234 6269 882
Size: 617 624 357 326 1060 368 2035 2219
Fragments arranged by size:
2219 2035 1060 624 617 368 357 326
BsaAI yAC'GTr
Cuts at: 493 1204 2704 3709 5397 493
Size: 711 1500 1005 1688 2702
Fragments arranged by size:
2702 1688 1500 1005 711
BsaBI GATnn'nnATC
Cuts at: 7600 7600
Size: 7606
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5413 5795 6430 275
Size: 53 83 186 936 3880 382 635 1451
Fragments arranged by size:
3880 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3636 4565 4635 6039 6437 6711 6858 7269 3636
Size: 929 70 1404 398 274 147 411 3973
Fragments arranged by size:
3973 1404 929 411 398 274 147 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2786 2816 4273 4303 1178
Size: 30 165 30 1383 30 1457 30 4481
Fragments arranged by size:
4481 1457 1383 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3404 3407 3938 4438 1186
Size: 2218 3 531 500 4354
Fragments arranged by size:
4354 2218 531 500 3
BseYI C'CCAG_C
Cuts at: 2672 3779 3858 4103 6809 7305 2672
Size: 1107 79 245 2706 496 2973
Fragments arranged by size:
2973 2706 1107 496 245 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2835 2985 1163
Size: 573 1099 150 5784
Fragments arranged by size:
5784 1099 573 150
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5056 5817 6845 1540
Size: 275 186 3055 761 1028 2301
Fragments arranged by size:
3055 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2421 3301 3501 4286 4535 5667 5752 6823 2421
Size: 880 200 785 249 1132 85 1071 3204
Fragments arranged by size:
3204 1132 1071 880 785 249 200 85
BsmI GAATG_Cn'
Cuts at: 2593 2881 4130 5117 5210 2593
Size: 288 1249 987 93 4989
Fragments arranged by size:
4989 1249 987 288 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2320 2421 3301 3469 3501 3980
Size: 399 313 217 101 880 168 32 479
Cuts at: 3980 4286 4465 4535 4759 4925 5667 5752 6823
Size: 306 179 70 224 166 742 85 1071
Cuts at: 6823 1391
Size: 2174
Fragments arranged by size:
2174 1071 880 742 479 399 313 306
224 217 179 168 166 101 85 70
32
BspEI T'CCGG_A
Cuts at: 3636 7269 3636
Size: 3633 3973
BspHI T'CATG_A
Cuts at: 5495 7225 5495
Size: 1730 5876
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2392 2470 3011 4961 844
Size: 1548 78 541 1950 3489
Fragments arranged by size:
3489 1950 1548 541 78
BsrBI CCG'CTC
Cuts at: 1394 1823 5493 1394
Size: 429 3670 3507
Fragments arranged by size:
3670 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6101 6275 65
Size: 1183 4853 174 1396
Fragments arranged by size:
4853 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2217 4565 6250 6437 1700
Size: 110 407 2348 1685 187 2869
Fragments arranged by size:
2869 2348 1685 407 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7606
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7606
BssSI C'ACGA_G
Cuts at: 5666 6678 5666
Size: 1012 6594
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2963 3296 794
Size: 2169 333 5104
Fragments arranged by size:
5104 2169 333
BstBI TT'CG_AA
Cuts at: 6410 6410
Size: 7606
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7606
BstXI CCAn_nnnn'nTGG
Cuts at: 3125 3125
Size: 7606
BstYI r'GATC_y
Cuts at: 1972 3847 4015 4936 5303 5688 5705 7157 7243
Size: 1875 168 921 367 385 17 1452 86
Cuts at: 7243 7255 7601 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1875 1452 921 385 367 346 168
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2864 3460 513
Size: 552 939 860 596 4659
Fragments arranged by size:
4659 939 860 596 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6867
Fragments arranged by size:
6867 683 56
BtsI GCAGTG_nn'
Cuts at: 2333 2823 2999 3913 5122 5916 5936 2333
Size: 490 176 914 1209 794 20 4003
Fragments arranged by size:
4003 1209 914 794 490 176 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7606
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7571
DraI TTT'AAA
Cuts at: 4414 4910 4414
Size: 496 7110
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3963 6613 809
Size: 507 2647 2650 1802
Fragments arranged by size:
2650 2647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2706 3232 3703
Size: 54 1425 50 275 18 876 526 471
Cuts at: 3703 5053 5942 7571 8
Size: 1350 889 1629 43
Fragments arranged by size:
1629 1425 1350 889 876 526 471 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5053 1537
Size: 275 3241 4090
Fragments arranged by size:
4090 3241 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3341 3578 3674 3864 4232 5536 1353
Size: 1988 237 96 190 368 1304 3423
Fragments arranged by size:
3423 1988 1304 368 237 190 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6193 6567 6713 387
Size: 1064 138 4604 374 146 1280
Fragments arranged by size:
4604 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4533 4533
Size: 7606
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2131 2246 3080 3188 3365 3659
Size: 393 431 68 115 834 108 177 294
Cuts at: 3659 3888 3945 4755 5669 6266 7053 7321 1239
Size: 229 57 810 914 597 787 268 1524
Fragments arranged by size:
1524 914 834 810 787 597 431 393
294 268 229 177 115 108 68 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2642 2642
Size: 7606
EcoO109I rG'GnC_Cy
Cuts at: 2080 2676 2758 2950 3956 2080
Size: 596 82 192 1006 5730
Fragments arranged by size:
5730 1006 596 192 82
EcoRI G'AATT_C
Cuts at: 4915 5293 4915
Size: 378 7228
EcoRV GAT'ATC
Cuts at: 3535 3535
Size: 7606
FspI TGC'GCA
Cuts at: 6112 6112
Size: 7606
HaeII r_GCGC'y
Cuts at: 1678 3566 4632 6753 1678
Size: 1888 1066 2121 2531
Fragments arranged by size:
2531 2121 1888 1066
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 2388 2420 2722
Size: 32 135 32 506 32 278 32 302
Cuts at: 2722 2754 2860 2892 3742 3774 4273 4305 4522
Size: 32 106 32 850 32 499 32 217
Cuts at: 4522 4554 6247 6279 6321 6353 1373
Size: 32 1693 32 42 32 2626
Fragments arranged by size:
2626 1693 850 506 499 302 278 217
135 106 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4950 5196 669
Size: 988 281 3012 246 3079
Fragments arranged by size:
3079 3012 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2187 4270 4435 748
Size: 1439 2083 165 3919
Fragments arranged by size:
3919 2083 1439 165
HpaI GTT'AAC
Cuts at: 5196 5196
Size: 7606
KpnI G_GTAC'C
Cuts at: 4931 4931
Size: 7606
MfeI C'AATT_G
Cuts at: 5205 5205
Size: 7606
MluI A'CGCG_T
Cuts at: 7482 7482
Size: 7606
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2826 4043 6720 6904 1305
Size: 1521 1217 2677 184 2007
Fragments arranged by size:
2677 2007 1521 1217 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2708 3234 3705 10
Size: 54 1425 343 876 526 471 3911
Fragments arranged by size:
3911 1425 876 526 471 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4566 5564 5923 6082 518
Size: 648 3400 998 359 159 2042
Fragments arranged by size:
3400 2042 998 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2551 5022 5699 6847 7092 1606
Size: 945 2471 677 1148 245 2120
Fragments arranged by size:
2471 2120 1148 945 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2864 3460 513
Size: 552 939 860 596 4659
Fragments arranged by size:
4659 939 860 596 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7606
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7606
NotI GC'GGCC_GC
Cuts at: 5053 5053
Size: 7606
NsiI A_TGCA'T
Cuts at: 4130 4130
Size: 7606
NspI r_CATG'y
Cuts at: 1132 1229 3241 4964 6509 1132
Size: 97 2012 1723 1545 2229
Fragments arranged by size:
2229 2012 1723 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2496 2604 2806 1348
Size: 1148 108 202 6148
Fragments arranged by size:
6148 1148 202 108
PciI A'CATG_T
Cuts at: 6505 6505
Size: 7606
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7606
PmeI GTTT'AAAC
Cuts at: 4910 4910
Size: 7606
PmlI CAC'GTG
Cuts at: 2704 3709 2704
Size: 1005 6601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2786 2818 3932 3964 5675 5707 7188 7220 2786
Size: 32 1114 32 1711 32 1481 32 3172
Fragments arranged by size:
3172 1711 1481 1114 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2080 2676 2758 2950 3956 2080
Size: 596 82 192 1006 5730
Fragments arranged by size:
5730 1006 596 192 82
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7606
PsiI TTA'TAA
Cuts at: 5176 5176
Size: 7606
PstI C_TGCA'G
Cuts at: 830 3292 3406 3975 4958 830
Size: 2462 114 569 983 3478
Fragments arranged by size:
3478 2462 983 569 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7606
PvuII CAG'CTG
Cuts at: 2551 2551
Size: 7606
SacI G_AGCT'C
Cuts at: 4535 4535
Size: 7606
SalI G'TCGA_C
Cuts at: 1936 4948 1936
Size: 3012 4594
SanDI GG'GwC_CC
Cuts at: 2676 2676
Size: 7606
SapI GCTCTTCn'nnn_
Cuts at: 3578 3578
Size: 7606
SbfI CC_TGCA'GG
Cuts at: 4958 4958
Size: 7606
ScaI AGT'ACT
Cuts at: 5854 7566 5854
Size: 1712 5894
SfcI C'TryA_G
Cuts at: 826 1045 2722 3288 3402 3603 3852 3971 4246
Size: 219 1677 566 114 201 249 119 275
Cuts at: 4246 4495 4954 6089 6770 6961 826
Size: 249 459 1135 681 191 1471
Fragments arranged by size:
1677 1471 1135 681 566 459 275 249
249 219 201 191 119 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7606
SgrAI Cr'CCGG_yG
Cuts at: 1700 4565 1700
Size: 2865 4741
SmaI CCC'GGG
Cuts at: 1872 4933 1872
Size: 3061 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2258 5710 6611 6873 820
Size: 197 926 315 3452 901 262 1553
Fragments arranged by size:
3452 1553 926 901 315 262 197
SnaBI TAC'GTA
Cuts at: 493 5397 493
Size: 4904 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7606
SphI G_CATG'C
Cuts at: 1132 4964 1132
Size: 3832 3774
SspI AAT'ATT
Cuts at: 5 52 5530 5
Size: 47 5478 2081
Fragments arranged by size:
5478 2081 47
StuI AGG'CCT
Cuts at: 1517 3101 4225 1517
Size: 1584 1124 4898
Fragments arranged by size:
4898 1584 1124
StyI C'CwwG_G
Cuts at: 513 1065 2004 2244 2864 3460 5045 513
Size: 552 939 240 620 596 1585 3074
Fragments arranged by size:
3074 1585 939 620 596 552 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5633 5650 5803 5988 6433 325
Size: 1255 4053 17 153 185 445 1498
Fragments arranged by size:
4053 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5852 7564 96
Size: 274 80 33 51 1443 3875 1712 138
Fragments arranged by size:
3875 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2072 2104 2650 2682 2802 2834 7566 7598 2072
Size: 32 546 32 120 32 4732 32 2080
Fragments arranged by size:
4732 2080 546 120 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7606
XbaI T'CTAG_A
Cuts at: 4942 4942
Size: 7606
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7606
XmaI C'CCGG_G
Cuts at: 1870 4931 1870
Size: 3061 4545
XmnI GAAnn'nnTTC
Cuts at: 3093 3564 3615 4152 4604 5735 3093
Size: 471 51 537 452 1131 4964
Fragments arranged by size:
4964 1131 537 471 452 51
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5414 276
Size: 53 83 186 936 3880 2468
Fragments arranged by size:
3880 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI
BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI
RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI