Restriction map for
pFN21AE3331
(Circular) (Six-base) MAPSORT of: pFN21AE3331.seq Check: 1515 from: 1 to: 7546
pFN21AE3331 7546 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5356 278
Size: 53 83 186 936 3820 2468
Fragments arranged by size:
3820 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4889 1937
Size: 2952 4594
Acc65I G'GTAC_C
Cuts at: 2367 4867 2367
Size: 2500 5046
AclI AA'CG_TT
Cuts at: 1563 5673 6046 1563
Size: 4110 373 3063
Fragments arranged by size:
4110 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2842 2926 3094 3514 3598 3682 4102 4522
Size: 1210 84 168 420 84 84 420 420
Cuts at: 4522 5609 6993 7261 1632
Size: 1087 1384 268 1917
Fragments arranged by size:
1917 1384 1210 1087 420 420 420 268
168 84 84 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7349
AflIII A'CryG_T
Cuts at: 1203 4843 6445 7422 1203
Size: 3640 1602 977 1327
Fragments arranged by size:
3640 1602 1327 977
AgeI A'CCGG_T
Cuts at: 6377 6377
Size: 7546
AhdI GACnn_n'nnGTC
Cuts at: 2124 6275 2124
Size: 4151 3395
AleI CACnn'nnGTG
Cuts at: 1166 2311 3069 4177 1166
Size: 1145 758 1108 4535
Fragments arranged by size:
4535 1145 1108 758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2329 2913 1184
Size: 1145 584 5817
Fragments arranged by size:
5817 1145 584
AlwNI CAG_nnn'CTG
Cuts at: 5289 6861 5289
Size: 1572 5974
ApaLI G'TGCA_C
Cuts at: 3986 4728 4768 5603 6759 3986
Size: 742 40 835 1156 4773
Fragments arranged by size:
4773 1156 835 742 40
ApoI r'AATT_y
Cuts at: 1493 2976 3060 3228 4236 4630 4855 5081 5233
Size: 1483 84 168 1008 394 225 226 152
Cuts at: 5233 7342 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1483 1008 394 226 225 168
152 84
AseI AT'TA_AT
Cuts at: 160 1032 6100 160
Size: 872 5068 1606
Fragments arranged by size:
5068 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7546
AvaI C'yCGr_G
Cuts at: 1870 1943 4871 1870
Size: 73 2928 4545
Fragments arranged by size:
4545 2928 73
AvrII C'CTAG_G
Cuts at: 2362 2362
Size: 7546
BamHI G'GATC_C
Cuts at: 4876 5243 4876
Size: 367 7179
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2367 4867 6322 618
Size: 325 217 627 580 2500 1455 1842
Fragments arranged by size:
2500 1842 1455 627 580 325 217
BanII G_rGCy'C
Cuts at: 1391 4865 1391
Size: 3474 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2561 3100 928
Size: 971 662 539 5374
Fragments arranged by size:
5374 971 662 539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2150 2184 5737 5771 1903
Size: 34 213 34 3553 34 3678
Fragments arranged by size:
3678 3553 213 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5438 6648 5438
Size: 1210 6336
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7546
BfrBI ATG'CAT
Cuts at: 4816 4816
Size: 7546
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6157 136
Size: 107 122 71 631 605 4485 1525
Fragments arranged by size:
4485 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7541 7541
Size: 7546
BlpI GC'TnA_GC
Cuts at: 4963 4963
Size: 7546
Bme1580I G_kGCm'C
Cuts at: 1790 3990 4732 4772 5607 6763 1790
Size: 2200 742 40 835 1156 2573
Fragments arranged by size:
2573 2200 1156 835 742 40
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7546
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5277 6235 442
Size: 416 53 83 399 24 3860 958 1753
Fragments arranged by size:
3860 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7546
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3599
Size: 7546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4014 6206 1239
Size: 2775 2192 2579
Fragments arranged by size:
2775 2579 2192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5671 6536 6834 5671
Size: 865 298 6383
Fragments arranged by size:
6383 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6209 882
Size: 617 4710 2219
Fragments arranged by size:
4710 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5337 493
Size: 711 4133 2702
Fragments arranged by size:
4133 2702 711
BsaBI GATnn'nnATC
Cuts at: 2496 7540 2496
Size: 5044 2502
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5353 5735 6370 275
Size: 53 83 186 936 3820 382 635 1451
Fragments arranged by size:
3820 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2019 5979 6377 6651 6798 7209 2019
Size: 3960 398 274 147 411 2356
Fragments arranged by size:
3960 2356 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7321
Fragments arranged by size:
7321 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 1186
Size: 1111 6435
BseYI C'CCAG_C
Cuts at: 2302 2518 4047 6749 7245 2302
Size: 216 1529 2702 496 2603
Fragments arranged by size:
2702 2603 1529 496 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2450 4201 4369 1163
Size: 573 714 1751 168 4340
Fragments arranged by size:
4340 1751 714 573 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4996 5757 6785 1540
Size: 275 186 2995 761 1028 2301
Fragments arranged by size:
2995 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3990 4732 4772 5607 5692 6763 3990
Size: 742 40 835 85 1071 4773
Fragments arranged by size:
4773 1071 835 742 85 40
BsmI GAATG_Cn'
Cuts at: 2549 3008 3017 3260 3596 3680 3764 4193 4268
Size: 459 9 243 336 84 84 429 75
Cuts at: 4268 4578 4604 5057 5150 2549
Size: 310 26 453 93 4945
Fragments arranged by size:
4945 459 453 429 336 310 243 93
84 84 75 26 9
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3990 4732 4772 4865 5607 5692 6763
Size: 399 2200 742 40 93 742 85 1071
Cuts at: 6763 1391
Size: 2174
Fragments arranged by size:
2200 2174 1071 742 742 399 93 85
40
BspEI T'CCGG_A
Cuts at: 2019 7209 2019
Size: 5190 2356
BspHI T'CATG_A
Cuts at: 5435 7165 5435
Size: 1730 5816
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4901 844
Size: 4057 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5433 1394
Size: 429 3610 3507
Fragments arranged by size:
3610 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3265 3517 4693 6041 6215 65
Size: 1183 2017 252 1176 1348 174 1396
Fragments arranged by size:
2017 1396 1348 1183 1176 252 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6190 6377 1700
Size: 110 4380 187 2869
Fragments arranged by size:
4380 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2319 2965 3385 3469 3889 3973 4057 4477
Size: 2223 646 420 84 420 84 84 420
Cuts at: 4477 4561 4645 96
Size: 84 84 2997
Fragments arranged by size:
2997 2223 646 420 420 420 84 84
84 84 84
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7546
BssSI C'ACGA_G
Cuts at: 5606 6618 5606
Size: 1012 6534
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3012 4188 794
Size: 2218 1176 4152
Fragments arranged by size:
4152 2218 1176
BstBI TT'CG_AA
Cuts at: 6350 6350
Size: 7546
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7546
BstXI CCAn_nnnn'nTGG
Cuts at: 3686 3686
Size: 7546
BstYI r'GATC_y
Cuts at: 1972 4876 5243 5628 5645 7097 7183 7195 7541
Size: 2904 367 385 17 1452 86 12 346
Cuts at: 7541 1972
Size: 1977
Fragments arranged by size:
2904 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6055
Fragments arranged by size:
6055 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6807
Fragments arranged by size:
6807 683 56
BtsI GCAGTG_nn'
Cuts at: 3013 4105 4273 4357 4609 5062 5856 5876 3013
Size: 1092 168 84 252 453 794 20 4683
Fragments arranged by size:
4683 1092 794 453 252 168 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7546
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3499 3534 3583 3618 4675 4710 529
Size: 35 2935 35 49 35 1057 35 3365
Fragments arranged by size:
3365 2935 1057 49 35 35 35 35
DraI TTT'AAA
Cuts at: 3201 3622 3789 4850 3201
Size: 421 167 1061 5897
Fragments arranged by size:
5897 1061 421 167
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6553 809
Size: 507 5237 1802
Fragments arranged by size:
5237 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 4993 5882
Size: 54 1425 50 275 18 177 2986 889
Cuts at: 5882 7511 8
Size: 1629 43
Fragments arranged by size:
2986 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4993 1537
Size: 275 3181 4090
Fragments arranged by size:
4090 3181 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2159 2409 2898 5476 1353
Size: 806 250 489 2578 3423
Fragments arranged by size:
3423 2578 806 489 250
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6133 6507 6653 387
Size: 1064 138 4544 374 146 1280
Fragments arranged by size:
4544 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2842 2926 3094 3514 3598 3682 4014
Size: 393 1210 84 168 420 84 84 332
Cuts at: 4014 4102 4522 5609 6206 6993 7261 1239
Size: 88 420 1087 597 787 268 1524
Fragments arranged by size:
1524 1210 1087 787 597 420 420 393
332 268 168 88 84 84 84
EcoNI CCTnn'n_nnAGG
Cuts at: 4288 4624 4288
Size: 336 7210
EcoO109I rG'GnC_Cy
Cuts at: 2038 2240 2038
Size: 202 7344
EcoRI G'AATT_C
Cuts at: 2976 3060 3228 4236 4630 4855 5233 2976
Size: 84 168 1008 394 225 378 5289
Fragments arranged by size:
5289 1008 394 378 225 168 84
EcoRV GAT'ATC
Cuts at: 2389 2389
Size: 7546
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3143 3143
Size: 7546
FspI TGC'GCA
Cuts at: 6052 6052
Size: 7546
HaeII r_GCGC'y
Cuts at: 1678 6693 1678
Size: 5015 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2110 2142 3877 3909 6187
Size: 32 135 32 538 32 1735 32 2278
Cuts at: 6187 6219 6261 6293 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2278 1735 538 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4504 4890 5136 669
Size: 988 281 2566 386 246 3079
Fragments arranged by size:
3079 2566 988 386 281 246
HindIII A'AGCT_T
Cuts at: 748 2536 3189 4365 748
Size: 1788 653 1176 3929
Fragments arranged by size:
3929 1788 1176 653
HpaI GTT'AAC
Cuts at: 5136 5136
Size: 7546
KpnI G_GTAC'C
Cuts at: 2371 4871 2371
Size: 2500 5046
MfeI C'AATT_G
Cuts at: 3598 5145 3598
Size: 1547 5999
MluI A'CGCG_T
Cuts at: 7422 7422
Size: 7546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2316 2382 3683 4775 6660 6844 1305
Size: 1011 66 1301 1092 1885 184 2007
Fragments arranged by size:
2007 1885 1301 1092 1011 184 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 5547
Fragments arranged by size:
5547 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2258 2311 2610 3069 3169 3253 3491
Size: 648 1092 53 299 459 100 84 238
Cuts at: 3491 3684 3841 4093 4177 4413 4429 4597 4833
Size: 193 157 252 84 236 16 168 236
Cuts at: 4833 5504 5863 6022 518
Size: 671 359 159 2042
Fragments arranged by size:
2042 1092 671 648 459 359 299 252
238 236 236 193 168 159 157 100
84 84 53 16
MspA1I CmG'CkG
Cuts at: 1606 2293 2841 4962 5639 6787 7032 1606
Size: 687 548 2121 677 1148 245 2120
Fragments arranged by size:
2121 2120 1148 687 677 548 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6055
Fragments arranged by size:
6055 939 552
NdeI CA'TA_TG
Cuts at: 387 3166 3250 4090 4146 4426 4594 387
Size: 2779 84 840 56 280 168 3339
Fragments arranged by size:
3339 2779 840 280 168 84 56
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7546
NotI GC'GGCC_GC
Cuts at: 4993 4993
Size: 7546
NsiI A_TGCA'T
Cuts at: 4818 4818
Size: 7546
NspI r_CATG'y
Cuts at: 1132 1229 4847 4904 6449 1132
Size: 97 3618 57 1545 2229
Fragments arranged by size:
3618 2229 1545 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7546
PciI A'CATG_T
Cuts at: 4843 6445 4843
Size: 1602 5944
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2235 1108
Size: 1127 6419
PmeI GTTT'AAAC
Cuts at: 4850 4850
Size: 7546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5615 5647 7128 7160 5615
Size: 32 1481 32 6001
Fragments arranged by size:
6001 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2038 2240 2038
Size: 202 7344
PshAI GACnn'nnGTC
Cuts at: 1613 2245 1613
Size: 632 6914
PsiI TTA'TAA
Cuts at: 4512 5116 4512
Size: 604 6942
PstI C_TGCA'G
Cuts at: 830 4898 830
Size: 4068 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7546
PvuII CAG'CTG
Cuts at: 2293 2841 2293
Size: 548 6998
SalI G'TCGA_C
Cuts at: 1936 4888 1936
Size: 2952 4594
SanDI GG'GwC_CC
Cuts at: 2038 2038
Size: 7546
SapI GCTCTTCn'nnn_
Cuts at: 2409 2409
Size: 7546
SbfI CC_TGCA'GG
Cuts at: 4898 4898
Size: 7546
ScaI AGT'ACT
Cuts at: 5794 7506 5794
Size: 1712 5834
SexAI A'CCwGG_T
Cuts at: 2352 2352
Size: 7546
SfcI C'TryA_G
Cuts at: 826 1045 2344 3922 4894 6029 6710 6901 826
Size: 219 1299 1578 972 1135 681 191 1471
Fragments arranged by size:
1578 1471 1299 1135 972 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7546
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7546
SmaI CCC'GGG
Cuts at: 1872 4873 1872
Size: 3001 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5650 6551 6813 820
Size: 197 926 3707 901 262 1553
Fragments arranged by size:
3707 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5337 493
Size: 4844 2702
SpeI A'CTAG_T
Cuts at: 152 2651 152
Size: 2499 5047
SphI G_CATG'C
Cuts at: 1132 4904 1132
Size: 3772 3774
SspI AAT'ATT
Cuts at: 5 52 5470 5
Size: 47 5418 2081
Fragments arranged by size:
5418 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7546
StyI C'CwwG_G
Cuts at: 513 1065 2004 2089 2113 2362 4985 513
Size: 552 939 85 24 249 2623 3074
Fragments arranged by size:
3074 2623 939 552 249 85 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2164 3411 4335 4671 5573 5590 5743
Size: 1255 584 1247 924 336 902 17 153
Cuts at: 5743 5928 6373 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1247 924 902 584 445 336
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2319 2576 2965
Size: 274 80 33 51 1443 342 257 389
Cuts at: 2965 3385 3469 3889 3973 4057 4477 4561 4645
Size: 420 84 420 84 84 420 84 84
Cuts at: 4645 5792 7504 96
Size: 1147 1712 138
Fragments arranged by size:
1712 1443 1147 420 420 420 389 342
274 257 138 84 84 84 84 84
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7506 7538 7506
Size: 32 7514
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7546
XbaI T'CTAG_A
Cuts at: 4882 4882
Size: 7546
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2120 2466 2120
Size: 346 7200
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7546
XmaI C'CCGG_G
Cuts at: 1870 4871 1870
Size: 3001 4545
XmnI GAAnn'nnTTC
Cuts at: 2062 2855 2939 3023 3107 3228 3611 3695 4115
Size: 793 84 84 84 121 383 84 420
Cuts at: 4115 4408 4451 4535 4660 4703 5675 2062
Size: 293 43 84 125 43 972 3933
Fragments arranged by size:
3933 972 793 420 383 293 125 121
84 84 84 84 84 43 43
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5354 276
Size: 53 83 186 936 3820 2468
Fragments arranged by size:
3820 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SfiI
SfoI SrfI SwaI