Restriction map for
pFN21AE3330
(Circular) (Six-base) MAPSORT of: pFN21AE3330.seq Check: 4391 from: 1 to: 7534
pFN21AE3330 7534 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4120 4543 4792 4120
Size: 423 249 6862
Fragments arranged by size:
6862 423 249
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5344 278
Size: 53 83 186 936 3808 2468
Fragments arranged by size:
3808 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2651 3465 4877 1937
Size: 714 814 1412 4594
Fragments arranged by size:
4594 1412 814 714
Acc65I G'GTAC_C
Cuts at: 4855 4855
Size: 7534
AclI AA'CG_TT
Cuts at: 1563 5661 6034 1563
Size: 4098 373 3063
Fragments arranged by size:
4098 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2082 2576 2831 3551 3646 4045 5597 6981
Size: 450 494 255 720 95 399 1552 1384
Cuts at: 6981 7249 1632
Size: 268 1917
Fragments arranged by size:
1917 1552 1384 720 494 450 399 268
255 95
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7337
AflIII A'CryG_T
Cuts at: 1203 6433 7410 1203
Size: 5230 977 1327
Fragments arranged by size:
5230 1327 977
AgeI A'CCGG_T
Cuts at: 3450 6365 3450
Size: 2915 4619
AhdI GACnn_n'nnGTC
Cuts at: 4030 6263 4030
Size: 2233 5301
AleI CACnn'nnGTG
Cuts at: 1166 4593 1166
Size: 3427 4107
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7534
AlwNI CAG_nnn'CTG
Cuts at: 2377 2393 3649 3669 4135 4772 5277 6849 2377
Size: 16 1256 20 466 637 505 1572 3062
Fragments arranged by size:
3062 1572 1256 637 505 466 20 16
ApaI G_GGCC'C
Cuts at: 3028 3128 3552 4371 4720 3028
Size: 100 424 819 349 5842
Fragments arranged by size:
5842 819 424 349 100
ApaLI G'TGCA_C
Cuts at: 2550 3782 5591 6747 2550
Size: 1232 1809 1156 3337
Fragments arranged by size:
3337 1809 1232 1156
ApoI r'AATT_y
Cuts at: 1493 2191 3692 4843 5069 5221 7330 1493
Size: 698 1501 1151 226 152 2109 1697
Fragments arranged by size:
2109 1697 1501 1151 698 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6088 160
Size: 872 5056 1606
Fragments arranged by size:
5056 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7534
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2733 3192 3493 4288 4859 1870
Size: 73 67 723 459 301 795 571 4545
Fragments arranged by size:
4545 795 723 571 459 301 73 67
BamHI G'GATC_C
Cuts at: 2119 4864 5231 2119
Size: 2745 367 4422
Fragments arranged by size:
4422 2745 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2737 4438 4483 4555 4855
Size: 325 217 627 950 1701 45 72 300
Cuts at: 4855 6310 618
Size: 1455 1842
Fragments arranged by size:
1842 1701 1455 950 627 325 300 217
72 45
BanII G_rGCy'C
Cuts at: 1391 2018 2888 3028 3128 3552 3771 4371 4720
Size: 627 870 140 100 424 219 600 349
Cuts at: 4720 4853 1391
Size: 133 4072
Fragments arranged by size:
4072 870 627 600 424 349 219 140
133 100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2348 2435 4051 928
Size: 971 449 87 1616 4411
Fragments arranged by size:
4411 1616 971 449 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5725 5759 1903
Size: 34 3788 34 3678
Fragments arranged by size:
3788 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5426 6636 5426
Size: 1210 6324
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7534
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 3624 3989
Size: 107 122 71 631 605 388 1564 365
Cuts at: 3989 4496 6145 136
Size: 507 1649 1525
Fragments arranged by size:
1649 1564 1525 631 605 507 388 365
122 107 71
BglII A'GATC_T
Cuts at: 3894 7529 3894
Size: 3635 3899
BlpI GC'TnA_GC
Cuts at: 4951 4951
Size: 7534
Bme1580I G_kGCm'C
Cuts at: 1790 2554 2740 3028 3090 3128 3552 3786 4371
Size: 764 186 288 62 38 424 234 585
Cuts at: 4371 4443 4720 5595 6751 1790
Size: 72 277 875 1156 2573
Fragments arranged by size:
2573 1156 875 764 585 424 288 277
234 186 72 62 38
BmgBI CAC'GTC
Cuts at: 1318 2649 3724 1318
Size: 1331 1075 5128
Fragments arranged by size:
5128 1331 1075
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4328 4396 5265
Size: 416 53 83 399 24 2911 68 869
Cuts at: 5265 6223 442
Size: 958 1753
Fragments arranged by size:
2911 1753 958 869 416 399 83 68
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7534
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4774 4774
Size: 7534
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2801 3785 6194 1239
Size: 980 582 984 2409 2579
Fragments arranged by size:
2579 2409 984 980 582
Bpu10I CC'TnA_GC
Cuts at: 2962 3072 3650 4322 2962
Size: 110 578 672 6174
Fragments arranged by size:
6174 672 578 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5659 6524 6822 5659
Size: 865 298 6371
Fragments arranged by size:
6371 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6197 882
Size: 617 4698 2219
Fragments arranged by size:
4698 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3835 4363 5325 493
Size: 711 2631 528 962 2702
Fragments arranged by size:
2702 2631 962 711 528
BsaBI GATnn'nnATC
Cuts at: 2333 7528 2333
Size: 5195 2339
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2448 5341 5723 6358
Size: 53 83 186 936 915 2893 382 635
Cuts at: 6358 275
Size: 1451
Fragments arranged by size:
2893 1451 936 915 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2337 2568 3450 4119 5967 6365 6639 6786 7197
Size: 231 882 669 1848 398 274 147 411
Cuts at: 7197 2337
Size: 2674
Fragments arranged by size:
2674 1848 882 669 411 398 274 231
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2714 2744 1178
Size: 30 165 30 1311 30 5968
Fragments arranged by size:
5968 1311 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3449 3575 4551 4757 4760 4763 1186
Size: 2263 126 976 206 3 3 3957
Fragments arranged by size:
3957 2263 976 206 126 3 3
BseYI C'CCAG_C
Cuts at: 2464 2920 2932 3233 3347 3439 3622 3949 4202
Size: 456 12 301 114 92 183 327 253
Cuts at: 4202 4580 4780 6737 7233 2464
Size: 378 200 1957 496 2765
Fragments arranged by size:
2765 1957 496 456 378 327 301 253
200 183 114 92 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2231 2333 4544 4751 1163
Size: 573 495 102 2211 207 3946
Fragments arranged by size:
3946 2211 573 495 207 102
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2036 2055 2067 4984 5745 6773
Size: 275 186 35 19 12 2917 761 1028
Cuts at: 6773 1540
Size: 2301
Fragments arranged by size:
2917 2301 1028 761 275 186 35 19
12
BsiHKAI G_wGCw'C
Cuts at: 2554 2675 3592 3786 4328 4727 5595 5680 6751
Size: 121 917 194 542 399 868 85 1071
Cuts at: 6751 2554
Size: 3337
Fragments arranged by size:
3337 1071 917 868 542 399 194 121
85
BsiWI C'GTAC_G
Cuts at: 3154 3154
Size: 7534
BsmI GAATG_Cn'
Cuts at: 5045 5138 5045
Size: 93 7441
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2554 2675 2740 2888 3028 3090
Size: 399 228 536 121 65 148 140 62
Cuts at: 3090 3128 3552 3592 3771 3786 4328 4371 4443
Size: 38 424 40 179 15 542 43 72
Cuts at: 4443 4720 4727 4853 5595 5680 6751 1391
Size: 277 7 126 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 542 536 424 399 277
228 179 148 140 126 121 85 72
65 62 43 40 38 15 7
BspEI T'CCGG_A
Cuts at: 2337 4119 7197 2337
Size: 1782 3078 2674
Fragments arranged by size:
3078 2674 1782
BspHI T'CATG_A
Cuts at: 4176 5423 7153 4176
Size: 1247 1730 4557
Fragments arranged by size:
4557 1730 1247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4120 4512 4543 4792 4889 844
Size: 3276 392 31 249 97 3489
Fragments arranged by size:
3489 3276 392 249 97 31
BsrBI CCG'CTC
Cuts at: 1394 1823 2820 2830 5421 1394
Size: 429 997 10 2591 3507
Fragments arranged by size:
3507 2591 997 429 10
BsrDI GCAATG_nn'
Cuts at: 65 1248 3868 4348 6029 6203 65
Size: 1183 2620 480 1681 174 1396
Fragments arranged by size:
2620 1681 1396 1183 480 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3450 4125 4206 4236 4480 4486 6178
Size: 110 1640 675 81 30 244 6 1692
Cuts at: 6178 6365 1700
Size: 187 2869
Fragments arranged by size:
2869 1692 1640 675 244 187 110 81
30 6
BsrGI T'GTAC_A
Cuts at: 96 3219 96
Size: 3123 4411
BssHII G'CGCG_C
Cuts at: 1925 2726 1925
Size: 801 6733
BssSI C'ACGA_G
Cuts at: 2510 3181 5594 6606 2510
Size: 671 2413 1012 3438
Fragments arranged by size:
3438 2413 1012 671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4135 4555 794
Size: 3341 420 3773
Fragments arranged by size:
3773 3341 420
BstBI TT'CG_AA
Cuts at: 6338 6338
Size: 7534
BstEII G'GTnAC_C
Cuts at: 1184 3473 3631 1184
Size: 2289 158 5087
Fragments arranged by size:
5087 2289 158
BstXI CCAn_nnnn'nTGG
Cuts at: 2516 2581 2516
Size: 65 7469
BstYI r'GATC_y
Cuts at: 1972 2119 3894 4527 4864 5231 5616 5633 7085
Size: 147 1775 633 337 367 385 17 1452
Cuts at: 7085 7171 7183 7529 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1775 1452 633 385 367 346 337
147 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2027 2321 3020 4434 4629 4650
Size: 552 939 23 294 699 1414 195 21
Cuts at: 4650 513
Size: 3397
Fragments arranged by size:
3397 1414 939 699 552 294 195 23
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2797 3223 3241 487
Size: 683 56 1571 426 18 4780
Fragments arranged by size:
4780 1571 683 426 56 18
BtsI GCAGTG_nn'
Cuts at: 2248 4606 5050 5844 5864 2248
Size: 2358 444 794 20 3918
Fragments arranged by size:
3918 2358 794 444 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7534
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7499
DraI TTT'AAA
Cuts at: 4838 4838
Size: 7534
DraIII CAC_nnn'GTG
Cuts at: 2707 3790 3835 2707
Size: 1083 45 6406
Fragments arranged by size:
6406 1083 45
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6541 809
Size: 507 5225 1802
Fragments arranged by size:
5225 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2033 2052 2064
Size: 54 1425 50 275 18 203 19 12
Cuts at: 2064 2412 3516 3852 4981 5870 7499 8
Size: 348 1104 336 1129 889 1629 43
Fragments arranged by size:
1629 1425 1129 1104 889 348 336 275
203 54 50 43 19 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2033 2052 2064 4981 1537
Size: 275 221 19 12 2917 4090
Fragments arranged by size:
4090 2917 275 221 19 12
EarI CTCTTCn'nnn_
Cuts at: 1353 3917 5464 1353
Size: 2564 1547 3423
Fragments arranged by size:
3423 2564 1547
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2341 3748 6121 6495 6641 387
Size: 1064 138 752 1407 2373 374 146 1280
Fragments arranged by size:
2373 1407 1280 1064 752 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2219 2576 2801 2831 3551 3646
Size: 393 450 137 357 225 30 720 95
Cuts at: 3646 3785 4045 5597 6194 6981 7249 1239
Size: 139 260 1552 597 787 268 1524
Fragments arranged by size:
1552 1524 787 720 597 450 393 357
268 260 225 139 137 95 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3557 4052 3557
Size: 495 7039
EcoO109I rG'GnC_Cy
Cuts at: 2138 2374 3025 3124 3125 3548 3549 3847 4367
Size: 236 651 99 1 423 1 298 520
Cuts at: 4367 4381 4497 4716 2138
Size: 14 116 219 4956
Fragments arranged by size:
4956 651 520 423 298 236 219 116
99 14 1 1
EcoRI G'AATT_C
Cuts at: 3692 4843 5221 3692
Size: 1151 378 6005
Fragments arranged by size:
6005 1151 378
FspI TGC'GCA
Cuts at: 2909 6040 2909
Size: 3131 4403
FspAI rTGC'GCAy
Cuts at: 2909 2909
Size: 7534
HaeII r_GCGC'y
Cuts at: 1678 2024 2870 4686 6681 1678
Size: 346 846 1816 1995 2531
Fragments arranged by size:
2531 1995 1816 846 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2155 2187 2352 2384 2657
Size: 32 135 32 583 32 165 32 273
Cuts at: 2657 2689 2714 2746 3164 3196 3806 3838 4016
Size: 32 25 32 418 32 610 32 178
Cuts at: 4016 4048 6175 6207 6249 6281 1373
Size: 32 2127 32 42 32 2626
Fragments arranged by size:
2626 2127 610 583 418 273 178 165
135 42 32 32 32 32 32 32
32 32 32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 4468 4878 5124 669
Size: 988 281 2530 410 246 3079
Fragments arranged by size:
3079 2530 988 410 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7534
HpaI GTT'AAC
Cuts at: 5124 5124
Size: 7534
KpnI G_GTAC'C
Cuts at: 4859 4859
Size: 7534
MfeI C'AATT_G
Cuts at: 5133 5133
Size: 7534
MluI A'CGCG_T
Cuts at: 7410 7410
Size: 7534
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2192 2495 2643 2847 4119 4287 4665 6648
Size: 887 303 148 204 1272 168 378 1983
Cuts at: 6648 6832 1305
Size: 184 2007
Fragments arranged by size:
2007 1983 1272 887 378 303 204 184
168 148
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2414 3518 3854 10
Size: 54 1425 343 582 1104 336 3690
Fragments arranged by size:
3690 1425 1104 582 343 336 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2514 2579 2837 3362 4412 4593 4649
Size: 648 1348 65 258 525 1050 181 56
Cuts at: 4649 5492 5851 6010 518
Size: 843 359 159 2042
Fragments arranged by size:
2042 1348 1050 843 648 525 359 258
181 159 65 56
MspA1I CmG'CkG
Cuts at: 1606 2029 2038 2323 2627 2851 3325 3334 3975
Size: 423 9 285 304 224 474 9 641
Cuts at: 3975 4825 4950 5627 6775 7020 1606
Size: 850 125 677 1148 245 2120
Fragments arranged by size:
2120 1148 850 677 641 474 423 304
285 245 224 125 9 9
NaeI GCC'GGC
Cuts at: 4208 4238 4488 4208
Size: 30 250 7254
Fragments arranged by size:
7254 250 30
NcoI C'CATG_G
Cuts at: 513 1065 2004 4434 4650 513
Size: 552 939 2430 216 3397
Fragments arranged by size:
3397 2430 939 552 216
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7534
NgoMIV G'CCGG_C
Cuts at: 4206 4236 4486 4206
Size: 30 250 7254
Fragments arranged by size:
7254 250 30
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7534
NotI GC'GGCC_GC
Cuts at: 2033 2064 4981 2033
Size: 31 2917 4586
Fragments arranged by size:
4586 2917 31
NspI r_CATG'y
Cuts at: 1132 1229 2908 3286 4892 6437 1132
Size: 97 1679 378 1606 1545 2229
Fragments arranged by size:
2229 1679 1606 1545 378 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7534
PciI A'CATG_T
Cuts at: 6433 6433
Size: 7534
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 2410 3358 3514 3835 4105 4712 1108
Size: 1269 33 948 156 321 270 607 3930
Fragments arranged by size:
3930 1269 948 607 321 270 156 33
PfoI T'CCnGG_A
Cuts at: 2090 2370 2090
Size: 280 7254
PmeI GTTT'AAAC
Cuts at: 4838 4838
Size: 7534
PmlI CAC'GTG
Cuts at: 3835 3835
Size: 7534
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2504 2536 5603 5635 7116 7148 2504
Size: 32 3067 32 1481 32 2890
Fragments arranged by size:
3067 2890 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2138 2374 3847 2138
Size: 236 1473 5825
Fragments arranged by size:
5825 1473 236
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7534
PsiI TTA'TAA
Cuts at: 5104 5104
Size: 7534
PspOMI G'GGCC_C
Cuts at: 3024 3124 3548 4367 4716 3024
Size: 100 424 819 349 5842
Fragments arranged by size:
5842 819 424 349 100
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3992 4024 3992
Size: 32 7502
PstI C_TGCA'G
Cuts at: 830 3033 4077 4803 4886 830
Size: 2203 1044 726 83 3478
Fragments arranged by size:
3478 2203 1044 726 83
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7534
PvuII CAG'CTG
Cuts at: 2851 3334 4825 2851
Size: 483 1491 5560
Fragments arranged by size:
5560 1491 483
SacII CC_GC'GG
Cuts at: 2030 2324 2030
Size: 294 7240
SalI G'TCGA_C
Cuts at: 1936 4876 1936
Size: 2940 4594
SbfI CC_TGCA'GG
Cuts at: 4886 4886
Size: 7534
ScaI AGT'ACT
Cuts at: 5782 7494 5782
Size: 1712 5822
SfcI C'TryA_G
Cuts at: 826 1045 3029 4073 4799 4882 6017 6698 6889
Size: 219 1984 1044 726 83 1135 681 191
Cuts at: 6889 826
Size: 1471
Fragments arranged by size:
1984 1471 1135 1044 726 681 219 191
83
SfiI GGCCn_nnn'nGGCC
Cuts at: 3989 4496 3989
Size: 507 7027
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7534
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7534
SmaI CCC'GGG
Cuts at: 1872 2012 2735 3495 4861 1872
Size: 140 723 760 1366 4545
Fragments arranged by size:
4545 1366 760 723 140
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5638 6539 6801 820
Size: 197 926 3695 901 262 1553
Fragments arranged by size:
3695 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5325 493
Size: 4832 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7534
SphI G_CATG'C
Cuts at: 1132 2908 4892 1132
Size: 1776 1984 3774
Fragments arranged by size:
3774 1984 1776
SspI AAT'ATT
Cuts at: 5 52 5458 5
Size: 47 5406 2081
Fragments arranged by size:
5406 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7534
StyI C'CwwG_G
Cuts at: 513 1065 2004 2227 3636 4434 4650 4973 513
Size: 552 939 223 1409 798 216 323 3074
Fragments arranged by size:
3074 1409 939 798 552 323 223 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2755 4153 5561 5578 5731 5916 6361
Size: 1255 1175 1398 1408 17 153 185 445
Cuts at: 6361 325
Size: 1498
Fragments arranged by size:
1498 1408 1398 1255 1175 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2805 3219 5780
Size: 274 80 33 51 1443 828 414 2561
Cuts at: 5780 7492 96
Size: 1712 138
Fragments arranged by size:
2561 1712 1443 828 414 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2086 2118 3038 3070 7494 7526 2086
Size: 32 920 32 4424 32 2094
Fragments arranged by size:
4424 2094 920 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7534
XbaI T'CTAG_A
Cuts at: 4870 4870
Size: 7534
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3355 3832 4102 4598 4648 3355
Size: 477 270 496 50 6241
Fragments arranged by size:
6241 496 477 270 50
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7534
XmaI C'CCGG_G
Cuts at: 1870 2010 2733 3493 4859 1870
Size: 140 723 760 1366 4545
Fragments arranged by size:
4545 1366 760 723 140
XmnI GAAnn'nnTTC
Cuts at: 4196 5663 4196
Size: 1467 6067
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5342 276
Size: 53 83 186 936 3808 2468
Fragments arranged by size:
3808 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI KasI
NarI NruI NsiI PacI PspXI RsrII SacI SanDI
SapI SexAI SfoI SrfI SwaI