Restriction map for
pFN21AE3329
(Circular) (Six-base) MAPSORT of: pFN21AE3329.seq Check: 9318 from: 1 to: 7516
pFN21AE3329 7516 bp
With 182 enzymes: SgfI *
March 28, 2012 12:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5326 278
Size: 53 83 186 936 3790 2468
Fragments arranged by size:
3790 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4589 4859 1937
Size: 2652 270 4594
Fragments arranged by size:
4594 2652 270
Acc65I G'GTAC_C
Cuts at: 4837 4837
Size: 7516
AclI AA'CG_TT
Cuts at: 1563 5643 6016 1563
Size: 4080 373 3063
Fragments arranged by size:
4080 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4008 4167 4272 5579 6963 7231 1632
Size: 2376 159 105 1307 1384 268 1917
Fragments arranged by size:
2376 1917 1384 1307 268 159 105
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7319
AflIII A'CryG_T
Cuts at: 1203 3337 4371 4665 6415 7392 1203
Size: 2134 1034 294 1750 977 1327
Fragments arranged by size:
2134 1750 1327 1034 977 294
AgeI A'CCGG_T
Cuts at: 6347 6347
Size: 7516
AhdI GACnn_n'nnGTC
Cuts at: 6245 6245
Size: 7516
AleI CACnn'nnGTG
Cuts at: 1166 2957 1166
Size: 1791 5725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7516
AlwNI CAG_nnn'CTG
Cuts at: 2882 5259 6831 2882
Size: 2377 1572 3567
Fragments arranged by size:
3567 2377 1572
ApaLI G'TGCA_C
Cuts at: 5573 6729 5573
Size: 1156 6360
ApoI r'AATT_y
Cuts at: 1493 2904 3016 3560 3921 4007 4825 5051 5203
Size: 1411 112 544 361 86 818 226 152
Cuts at: 5203 7312 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1411 818 544 361 226 152
112 86
AseI AT'TA_AT
Cuts at: 160 1032 3669 6070 160
Size: 872 2637 2401 1606
Fragments arranged by size:
2637 2401 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7516
AvaI C'yCGr_G
Cuts at: 1870 1943 4378 4841 1870
Size: 73 2435 463 4545
Fragments arranged by size:
4545 2435 463 73
BamHI G'GATC_C
Cuts at: 4628 4846 5213 4628
Size: 218 367 6931
Fragments arranged by size:
6931 367 218
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2997 3313 3623 4837 6292
Size: 325 217 627 1210 316 310 1214 1455
Cuts at: 6292 618
Size: 1842
Fragments arranged by size:
1842 1455 1214 1210 627 325 316 310
217
BanII G_rGCy'C
Cuts at: 1391 2310 2876 4835 1391
Size: 919 566 1959 4072
Fragments arranged by size:
4072 1959 919 566
BbeI G_GCGC'C
Cuts at: 3001 3001
Size: 7516
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4155 928
Size: 971 2256 4289
Fragments arranged by size:
4289 2256 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5707 5741 1903
Size: 34 50 34 3686 34 3678
Fragments arranged by size:
3686 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2474 2903 3036 4460 5408 6618 2474
Size: 429 133 1424 948 1210 3372
Fragments arranged by size:
3372 1424 1210 948 429 133
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7516
BfrBI ATG'CAT
Cuts at: 3377 3377
Size: 7516
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2314 6127 136
Size: 107 122 71 631 605 642 3813 1525
Fragments arranged by size:
3813 1525 642 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2854 7511 2854
Size: 4657 2859
BlpI GC'TnA_GC
Cuts at: 3504 4933 3504
Size: 1429 6087
Bme1580I G_kGCm'C
Cuts at: 1790 5577 6733 1790
Size: 3787 1156 2573
Fragments arranged by size:
3787 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2211 3340 4234 1318
Size: 893 1129 894 4600
Fragments arranged by size:
4600 1129 894 893
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3016 4328 5247
Size: 416 53 83 399 24 1599 1312 919
Cuts at: 5247 6205 442
Size: 958 1753
Fragments arranged by size:
1753 1599 1312 958 919 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7516
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4276 4276
Size: 7516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2454 2789 4058 6176 1239
Size: 1215 335 1269 2118 2579
Fragments arranged by size:
2579 2118 1269 1215 335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2134 4246 5641 6506 6804 2134
Size: 2112 1395 865 298 2846
Fragments arranged by size:
2846 2112 1395 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4204 6179 882
Size: 617 2705 1975 2219
Fragments arranged by size:
2705 2219 1975 617
BsaAI yAC'GTr
Cuts at: 493 1204 5307 493
Size: 711 4103 2702
Fragments arranged by size:
4103 2702 711
BsaBI GATnn'nnATC
Cuts at: 7510 7510
Size: 7516
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2998 4770 5323 5705
Size: 53 83 186 936 1465 1772 553 382
Cuts at: 5705 6340 275
Size: 635 1451
Fragments arranged by size:
1772 1465 1451 936 635 553 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2895 5949 6347 6621 6768 7179 2895
Size: 3054 398 274 147 411 3232
Fragments arranged by size:
3232 3054 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4510 4540 1178
Size: 30 165 30 3107 30 4154
Fragments arranged by size:
4154 3107 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7516
BseYI C'CCAG_C
Cuts at: 2862 3169 3546 6719 7215 2862
Size: 307 377 3173 496 3163
Fragments arranged by size:
3173 3163 496 377 307
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 3578 3767 1163
Size: 573 483 1359 189 4912
Fragments arranged by size:
4912 1359 573 483 189
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2015 4966 5727 6755 1540
Size: 275 186 14 2951 761 1028 2301
Fragments arranged by size:
2951 2301 1028 761 275 186 14
BsiHKAI G_wGCw'C
Cuts at: 5577 5662 6733 5577
Size: 85 1071 6360
Fragments arranged by size:
6360 1071 85
BsmI GAATG_Cn'
Cuts at: 3379 5027 5120 3379
Size: 1648 93 5775
Fragments arranged by size:
5775 1648 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2217 4761 2217
Size: 2544 4972
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2310 2876 4835 5577 5662 6733 1391
Size: 399 520 566 1959 742 85 1071 2174
Fragments arranged by size:
2174 1959 1071 742 566 520 399 85
BspEI T'CCGG_A
Cuts at: 2895 7179 2895
Size: 4284 3232
BspHI T'CATG_A
Cuts at: 3699 4176 5405 7135 3699
Size: 477 1229 1730 4080
Fragments arranged by size:
4080 1730 1229 477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4871 844
Size: 4027 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2041 5403 1394
Size: 429 218 3362 3507
Fragments arranged by size:
3507 3362 429 218
BsrDI GCAATG_nn'
Cuts at: 65 1248 3255 3557 3786 6011 6185 65
Size: 1183 2007 302 229 2225 174 1396
Fragments arranged by size:
2225 2007 1396 1183 302 229 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2010 6160 6347 1700
Size: 110 200 4150 187 2869
Fragments arranged by size:
4150 2869 200 187 110
BsrGI T'GTAC_A
Cuts at: 96 3219 4167 96
Size: 3123 948 3445
Fragments arranged by size:
3445 3123 948
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7516
BssSI C'ACGA_G
Cuts at: 5576 6588 5576
Size: 1012 6504
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7516
BstBI TT'CG_AA
Cuts at: 6320 6320
Size: 7516
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7516
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 3250 4412 2215
Size: 1035 1162 5319
Fragments arranged by size:
5319 1162 1035
BstYI r'GATC_y
Cuts at: 1972 2854 2917 3679 3704 4628 4846 5213 5598
Size: 882 63 762 25 924 218 367 385
Cuts at: 5598 5615 7067 7153 7165 7511 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 924 882 762 385 367 346
218 86 63 25 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2023 2910 3011 3769 513
Size: 552 958 887 101 758 4260
Fragments arranged by size:
4260 958 887 758 552 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2724 2766 487
Size: 683 56 1498 42 5237
Fragments arranged by size:
5237 1498 683 56 42
BtsI GCAGTG_nn'
Cuts at: 2236 2466 3739 4543 4582 5032 5826 5846 2236
Size: 230 1273 804 39 450 794 20 3906
Fragments arranged by size:
3906 1273 804 794 450 230 39 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7516
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7481
DraI TTT'AAA
Cuts at: 4222 4820 4222
Size: 598 6918
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4263 6523 809
Size: 507 2947 2260 1802
Fragments arranged by size:
2947 2260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2012 2055 2070
Size: 54 1425 50 275 18 182 43 15
Cuts at: 2070 3240 3252 4600 4963 5852 7481 8
Size: 1170 12 1348 363 889 1629 43
Fragments arranged by size:
1629 1425 1348 1170 889 363 275 182
54 50 43 43 18 15 12
EagI C'GGCC_G
Cuts at: 1537 1812 2012 4963 1537
Size: 275 200 2951 4090
Fragments arranged by size:
4090 2951 275 200
EarI CTCTTCn'nnn_
Cuts at: 1353 3272 4018 5446 1353
Size: 1919 746 1428 3423
Fragments arranged by size:
3423 1919 1428 746
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6103 6477 6623 387
Size: 1064 138 4514 374 146 1280
Fragments arranged by size:
4514 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2454 2789 4008 4058 4167 4272 5579
Size: 393 822 335 1219 50 109 105 1307
Cuts at: 5579 6176 6963 7231 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1307 1219 822 787 597 393 335
268 109 105 50
EcoO109I rG'GnC_Cy
Cuts at: 3847 4523 3847
Size: 676 6840
EcoRI G'AATT_C
Cuts at: 2904 3016 4825 5203 2904
Size: 112 1809 378 5217
Fragments arranged by size:
5217 1809 378 112
EcoRV GAT'ATC
Cuts at: 4027 4361 4027
Size: 334 7182
FspI TGC'GCA
Cuts at: 6022 6022
Size: 7516
HaeII r_GCGC'y
Cuts at: 1678 2051 3001 6663 1678
Size: 373 950 3662 2531
Fragments arranged by size:
3662 2531 950 373
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4016 4048 6157 6189 6231
Size: 32 135 32 2444 32 2109 32 42
Cuts at: 6231 6263 1373
Size: 32 2626
Fragments arranged by size:
2626 2444 2109 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4590 4860 5106 669
Size: 988 281 2652 270 246 3079
Fragments arranged by size:
3079 2652 988 281 270 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7516
HpaI GTT'AAC
Cuts at: 5106 5106
Size: 7516
KasI G'GCGC_C
Cuts at: 2997 2997
Size: 7516
KpnI G_GTAC'C
Cuts at: 4841 4841
Size: 7516
MfeI C'AATT_G
Cuts at: 5115 5115
Size: 7516
MluI A'CGCG_T
Cuts at: 7392 7392
Size: 7516
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3269 6630 6814 1305
Size: 1964 3361 184 2007
Fragments arranged by size:
3361 2007 1964 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3242 3254 4602 10
Size: 54 1425 343 1410 12 1348 2924
Fragments arranged by size:
2924 1425 1410 1348 343 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2957 3227 3717 4686 5474 5833 5992
Size: 648 1791 270 490 969 788 359 159
Cuts at: 5992 518
Size: 2042
Fragments arranged by size:
2042 1791 969 788 648 490 359 270
159
MspA1I CmG'CkG
Cuts at: 1606 2025 2274 2615 3508 3806 3932 4502 4932
Size: 419 249 341 893 298 126 570 430
Cuts at: 4932 5609 6757 7002 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 893 677 570 430 419 341
298 249 245 126
NarI GG'CG_CC
Cuts at: 2998 2998
Size: 7516
NcoI C'CATG_G
Cuts at: 513 1065 2910 3011 513
Size: 552 1845 101 5018
Fragments arranged by size:
5018 1845 552 101
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7516
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7516
NotI GC'GGCC_GC
Cuts at: 4963 4963
Size: 7516
NruI TCG'CGA
Cuts at: 2751 2751
Size: 7516
NsiI A_TGCA'T
Cuts at: 3379 3379
Size: 7516
NspI r_CATG'y
Cuts at: 1132 1229 2538 2587 3655 4375 4669 4874 6419
Size: 97 1309 49 1068 720 294 205 1545
Cuts at: 6419 1132
Size: 2229
Fragments arranged by size:
2229 1545 1309 1068 720 294 205 97
49
PasI CC'CwG_GG
Cuts at: 1348 3098 1348
Size: 1750 5766
PciI A'CATG_T
Cuts at: 4371 4665 6415 4371
Size: 294 1750 5472
Fragments arranged by size:
5472 1750 294
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2274 2398 3975 4085 1108
Size: 1166 124 1577 110 4539
Fragments arranged by size:
4539 1577 1166 124 110
PfoI T'CCnGG_A
Cuts at: 2301 2476 2766 4480 2301
Size: 175 290 1714 5337
Fragments arranged by size:
5337 1714 290 175
PmeI GTTT'AAAC
Cuts at: 4820 4820
Size: 7516
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5585 5617 7098 7130 5585
Size: 32 1481 32 5971
Fragments arranged by size:
5971 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3847 4523 3847
Size: 676 6840
PshAI GACnn'nnGTC
Cuts at: 1613 2745 1613
Size: 1132 6384
PsiI TTA'TAA
Cuts at: 2946 5086 2946
Size: 2140 5376
PstI C_TGCA'G
Cuts at: 830 3731 4810 4868 830
Size: 2901 1079 58 3478
Fragments arranged by size:
3478 2901 1079 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7516
PvuII CAG'CTG
Cuts at: 2615 3508 3806 4502 2615
Size: 893 298 696 5629
Fragments arranged by size:
5629 893 696 298
SacII CC_GC'GG
Cuts at: 2026 2026
Size: 7516
SalI G'TCGA_C
Cuts at: 1936 4588 4858 1936
Size: 2652 270 4594
Fragments arranged by size:
4594 2652 270
SbfI CC_TGCA'GG
Cuts at: 4868 4868
Size: 7516
ScaI AGT'ACT
Cuts at: 5764 7476 5764
Size: 1712 5804
SfcI C'TryA_G
Cuts at: 826 1045 3727 3940 4806 4864 5999 6680 6871
Size: 219 2682 213 866 58 1135 681 191
Cuts at: 6871 826
Size: 1471
Fragments arranged by size:
2682 1471 1135 866 681 219 213 191
58
SfoI GGC'GCC
Cuts at: 2999 2999
Size: 7516
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7516
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7516
SmaI CCC'GGG
Cuts at: 1872 4843 1872
Size: 2971 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2149 4261 5620 6521 6783 820
Size: 197 926 206 2112 1359 901 262 1553
Fragments arranged by size:
2112 1553 1359 926 901 262 206 197
SnaBI TAC'GTA
Cuts at: 493 5307 493
Size: 4814 2702
SpeI A'CTAG_T
Cuts at: 152 3226 152
Size: 3074 4442
SphI G_CATG'C
Cuts at: 1132 3655 4874 1132
Size: 2523 1219 3774
Fragments arranged by size:
3774 2523 1219
SspI AAT'ATT
Cuts at: 5 52 3607 3865 4648 4777 5440 5
Size: 47 3555 258 783 129 663 2081
Fragments arranged by size:
3555 2081 783 663 258 129 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7516
StyI C'CwwG_G
Cuts at: 513 1065 2910 3011 3132 3192 4955 513
Size: 552 1845 101 121 60 1763 3074
Fragments arranged by size:
3074 1845 1763 552 121 101 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2316 3431 4174 4233 4554 5543 5560
Size: 1255 736 1115 743 59 321 989 17
Cuts at: 5560 5713 5898 6343 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1115 989 743 736 445 321
185 153 59 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2793 3219 4167
Size: 274 80 33 51 1443 816 426 948
Cuts at: 4167 5762 7474 96
Size: 1595 1712 138
Fragments arranged by size:
1712 1595 1443 948 816 426 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2458 2490 2521 2553 2965 2997 7476 7508 2458
Size: 32 31 32 412 32 4479 32 2466
Fragments arranged by size:
4479 2466 412 32 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7516
XbaI T'CTAG_A
Cuts at: 4852 4852
Size: 7516
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4082 4082
Size: 7516
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7516
XmaI C'CCGG_G
Cuts at: 1870 4841 1870
Size: 2971 4545
XmnI GAAnn'nnTTC
Cuts at: 5645 5645
Size: 7516
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5324 276
Size: 53 83 186 936 3790 2468
Fragments arranged by size:
3790 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI
FseI FspAI NaeI NgoMIV PacI PmlI PspOMI PspXI
PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI
SwaI