Restriction map for
pFN21AE3328
(Circular) (Six-base) MAPSORT of: pFN21AE3328.seq Check: 7547 from: 1 to: 7513
pFN21AE3328 7513 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5323 278
Size: 53 83 186 936 3787 2468
Fragments arranged by size:
3787 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4856 1937
Size: 2919 4594
Acc65I G'GTAC_C
Cuts at: 4834 4834
Size: 7513
AclI AA'CG_TT
Cuts at: 1563 3778 5640 6013 1563
Size: 2215 1862 373 3063
Fragments arranged by size:
3063 2215 1862 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2455 3202 3530 3665 4237 4446 4467 5576
Size: 823 747 328 135 572 209 21 1109
Cuts at: 5576 6960 7228 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1109 823 747 572 328 268
209 135 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7316
AflIII A'CryG_T
Cuts at: 1203 3458 6412 7389 1203
Size: 2255 2954 977 1327
Fragments arranged by size:
2954 2255 1327 977
AgeI A'CCGG_T
Cuts at: 6344 6344
Size: 7513
AhdI GACnn_n'nnGTC
Cuts at: 6242 6242
Size: 7513
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7513
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7513
AlwNI CAG_nnn'CTG
Cuts at: 2138 4153 4322 5256 6828 2138
Size: 2015 169 934 1572 2823
Fragments arranged by size:
2823 2015 1572 934 169
ApaLI G'TGCA_C
Cuts at: 4222 5570 6726 4222
Size: 1348 1156 5009
Fragments arranged by size:
5009 1348 1156
ApoI r'AATT_y
Cuts at: 1493 2108 2317 2870 3152 3275 4278 4293 4439
Size: 615 209 553 282 123 1003 15 146
Cuts at: 4439 4822 5048 5200 7309 1493
Size: 383 226 152 2109 1697
Fragments arranged by size:
2109 1697 1003 615 553 383 282 226
209 152 146 123 15
AseI AT'TA_AT
Cuts at: 160 1032 2336 3998 6067 160
Size: 872 1304 1662 2069 1606
Fragments arranged by size:
2069 1662 1606 1304 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7513
AvaI C'yCGr_G
Cuts at: 1870 1943 4838 1870
Size: 73 2895 4545
Fragments arranged by size:
4545 2895 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4394 4427 4394
Size: 33 7480
BamHI G'GATC_C
Cuts at: 2233 4843 5210 2233
Size: 2610 367 4536
Fragments arranged by size:
4536 2610 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4834 6289 618
Size: 325 217 627 3047 1455 1842
Fragments arranged by size:
3047 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2583 4763 4832 1391
Size: 1192 2180 69 4072
Fragments arranged by size:
4072 2180 1192 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3015 4167 4243 4438 4703 928
Size: 971 1116 1152 76 195 265 3738
Fragments arranged by size:
3738 1152 1116 971 265 195 76
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4208 4242 5704 5738 1903
Size: 34 2271 34 1462 34 3678
Fragments arranged by size:
3678 2271 1462 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2693 4756 5405 6615 2693
Size: 2063 649 1210 3591
Fragments arranged by size:
3591 2063 1210 649
BclI T'GATC_A
Cuts at: 1548 2655 3286 1548
Size: 1107 631 5775
Fragments arranged by size:
5775 1107 631
BfrBI ATG'CAT
Cuts at: 4768 4768
Size: 7513
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6124 136
Size: 107 122 71 631 605 4452 1525
Fragments arranged by size:
4452 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7508 7508
Size: 7513
BlpI GC'TnA_GC
Cuts at: 4930 4930
Size: 7513
Bme1580I G_kGCm'C
Cuts at: 1790 2281 3330 4226 5574 6730 1790
Size: 491 1049 896 1348 1156 2573
Fragments arranged by size:
2573 1348 1156 1049 896 491
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7513
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5244 6202 442
Size: 416 53 83 399 24 3827 958 1753
Fragments arranged by size:
3827 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7513
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2262 3585 6173 1239
Size: 1023 1323 2588 2579
Fragments arranged by size:
2588 2579 1323 1023
Bpu10I CC'TnA_GC
Cuts at: 3718 3718
Size: 7513
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2982 4295 4662 5638 6503 6801 2982
Size: 1313 367 976 865 298 3694
Fragments arranged by size:
3694 1313 976 865 367 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3517 4527 6176 882
Size: 617 2018 1010 1649 2219
Fragments arranged by size:
2219 2018 1649 1010 617
BsaAI yAC'GTr
Cuts at: 493 1204 2102 5304 493
Size: 711 898 3202 2702
Fragments arranged by size:
3202 2702 898 711
BsaBI GATnn'nnATC
Cuts at: 3405 7507 3405
Size: 4102 3411
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5320 5702 6337 275
Size: 53 83 186 936 3787 382 635 1451
Fragments arranged by size:
3787 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2026 5946 6344 6618 6765 7176 2026
Size: 3920 398 274 147 411 2363
Fragments arranged by size:
3920 2363 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2769 2799 1178
Size: 30 165 30 1366 30 5892
Fragments arranged by size:
5892 1366 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3582 3635 4709 1186
Size: 2396 53 1074 3990
Fragments arranged by size:
3990 2396 1074 53
BseYI C'CCAG_C
Cuts at: 2052 3425 3902 6716 7212 2052
Size: 1373 477 2814 496 2353
Fragments arranged by size:
2814 2353 1373 496 477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6940
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4963 5724 6752 1540
Size: 275 186 2962 761 1028 2301
Fragments arranged by size:
2962 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4226 4763 5574 5659 6730 4226
Size: 537 811 85 1071 5009
Fragments arranged by size:
5009 1071 811 537 85
BsmI GAATG_Cn'
Cuts at: 2998 5024 5117 2998
Size: 2026 93 5394
Fragments arranged by size:
5394 2026 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2281 2583 3330 4226 4763 4832 5574
Size: 399 491 302 747 896 537 69 742
Cuts at: 5574 5659 6730 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 896 747 742 537 491 399
302 85 69
BspEI T'CCGG_A
Cuts at: 2026 7176 2026
Size: 5150 2363
BspHI T'CATG_A
Cuts at: 3961 4455 5402 7132 3961
Size: 494 947 1730 4342
Fragments arranged by size:
4342 1730 947 494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2418 2683 3420 4868 844
Size: 1574 265 737 1448 3489
Fragments arranged by size:
3489 1574 1448 737 265
BsrBI CCG'CTC
Cuts at: 1394 1823 5400 1394
Size: 429 3577 3507
Fragments arranged by size:
3577 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2816 6008 6182 65
Size: 1183 1568 3192 174 1396
Fragments arranged by size:
3192 1568 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6157 6344 1700
Size: 110 4347 187 2869
Fragments arranged by size:
4347 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7513
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7513
BssSI C'ACGA_G
Cuts at: 5573 6585 5573
Size: 1012 6501
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7513
BstBI TT'CG_AA
Cuts at: 2560 4608 6317 2560
Size: 2048 1709 3756
Fragments arranged by size:
3756 2048 1709
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7513
BstXI CCAn_nnnn'nTGG
Cuts at: 4027 4661 4027
Size: 634 6879
BstYI r'GATC_y
Cuts at: 1972 2233 4843 5210 5595 5612 7064 7150 7162
Size: 261 2610 367 385 17 1452 86 12
Cuts at: 7162 7508 1972
Size: 346 1977
Fragments arranged by size:
2610 1977 1452 385 367 346 261 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4206 4206
Size: 7513
BtgI C'CryG_G
Cuts at: 513 1065 2004 3355 513
Size: 552 939 1351 4671
Fragments arranged by size:
4671 1351 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6774
Fragments arranged by size:
6774 683 56
BtsI GCAGTG_nn'
Cuts at: 2712 4226 4522 5029 5823 5843 2712
Size: 1514 296 507 794 20 4382
Fragments arranged by size:
4382 1514 794 507 296 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7513
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7478
DraI TTT'AAA
Cuts at: 2487 4817 2487
Size: 2330 5183
DraIII CAC_nnn'GTG
Cuts at: 2276 4221 2276
Size: 1945 5568
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6520 809
Size: 507 5204 1802
Fragments arranged by size:
5204 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2740 4573 4960
Size: 54 1425 50 275 18 910 1833 387
Cuts at: 4960 5849 7478 8
Size: 889 1629 43
Fragments arranged by size:
1833 1629 1425 910 889 387 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4960 1537
Size: 275 3148 4090
Fragments arranged by size:
4090 3148 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3965 4461 5443 1353
Size: 2612 496 982 3423
Fragments arranged by size:
3423 2612 982 496
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2573 6100 6474 6620 387
Size: 1064 138 984 3527 374 146 1280
Fragments arranged by size:
3527 1280 1064 984 374 146 138
EcoICRI GAG'CTC
Cuts at: 4761 4761
Size: 7513
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2262 2455 3202 3530 3585 3665 4237
Size: 393 630 193 747 328 55 80 572
Cuts at: 4237 4446 4467 5576 6173 6960 7228 1239
Size: 209 21 1109 597 787 268 1524
Fragments arranged by size:
1524 1109 787 747 630 597 572 393
328 268 209 193 80 55 21
EcoO109I rG'GnC_Cy
Cuts at: 2020 4568 4635 2020
Size: 2548 67 4898
Fragments arranged by size:
4898 2548 67
EcoRI G'AATT_C
Cuts at: 2108 2317 4822 5200 2108
Size: 209 2505 378 4421
Fragments arranged by size:
4421 2505 378 209
FspI TGC'GCA
Cuts at: 6019 6019
Size: 7513
HaeII r_GCGC'y
Cuts at: 1678 6660 1678
Size: 4982 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2647 2679 2769 2801 3974
Size: 32 135 32 1075 32 90 32 1173
Cuts at: 3974 4006 4478 4510 6154 6186 6228 6260 1373
Size: 32 472 32 1644 32 42 32 2626
Fragments arranged by size:
2626 1644 1173 1075 472 135 90 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4857 5103 669
Size: 988 281 2919 246 3079
Fragments arranged by size:
3079 2919 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7513
HpaI GTT'AAC
Cuts at: 5103 5103
Size: 7513
KpnI G_GTAC'C
Cuts at: 4838 4838
Size: 7513
MfeI C'AATT_G
Cuts at: 5112 5112
Size: 7513
MluI A'CGCG_T
Cuts at: 7389 7389
Size: 7513
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2677 2884 4175 6627 6811 1305
Size: 1372 207 1291 2452 184 2007
Fragments arranged by size:
2452 2007 1372 1291 207 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2742 10
Size: 54 1425 343 910 4781
Fragments arranged by size:
4781 1425 910 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2346 5471 5830 5989 518
Size: 648 1180 3125 359 159 2042
Fragments arranged by size:
3125 2042 1180 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2135 2173 4319 4929 5606 6754 6999
Size: 406 123 38 2146 610 677 1148 245
Cuts at: 6999 1606
Size: 2120
Fragments arranged by size:
2146 2120 1148 677 610 406 245 123
38
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6022
Fragments arranged by size:
6022 939 552
NdeI CA'TA_TG
Cuts at: 387 2377 387
Size: 1990 5523
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7513
NotI GC'GGCC_GC
Cuts at: 4960 4960
Size: 7513
NsiI A_TGCA'T
Cuts at: 4770 4770
Size: 7513
NspI r_CATG'y
Cuts at: 1132 1229 3141 3462 4112 4422 4871 6416 1132
Size: 97 1912 321 650 310 449 1545 2229
Fragments arranged by size:
2229 1912 1545 650 449 321 310 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7513
PciI A'CATG_T
Cuts at: 3458 6412 3458
Size: 2954 4559
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7513
PmeI GTTT'AAAC
Cuts at: 4817 4817
Size: 7513
PmlI CAC'GTG
Cuts at: 2102 2102
Size: 7513
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5582 5614 7095 7127 5582
Size: 32 1481 32 5968
Fragments arranged by size:
5968 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2020 4568 4635 2020
Size: 2548 67 4898
Fragments arranged by size:
4898 2548 67
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7513
PsiI TTA'TAA
Cuts at: 3519 5083 3519
Size: 1564 5949
PstI C_TGCA'G
Cuts at: 830 2415 4865 830
Size: 1585 2450 3478
Fragments arranged by size:
3478 2450 1585
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7513
PvuII CAG'CTG
Cuts at: 2012 2173 2012
Size: 161 7352
SacI G_AGCT'C
Cuts at: 4763 4763
Size: 7513
SalI G'TCGA_C
Cuts at: 1936 4855 1936
Size: 2919 4594
SanDI GG'GwC_CC
Cuts at: 2020 2020
Size: 7513
SbfI CC_TGCA'GG
Cuts at: 4865 4865
Size: 7513
ScaI AGT'ACT
Cuts at: 5761 7473 5761
Size: 1712 5801
SfcI C'TryA_G
Cuts at: 826 1045 2411 4145 4861 5996 6677 6868 826
Size: 219 1366 1734 716 1135 681 191 1471
Fragments arranged by size:
1734 1471 1366 1135 716 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7513
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7513
SmaI CCC'GGG
Cuts at: 1872 4840 1872
Size: 2968 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2997 4310 4677 5617 6518 6780
Size: 197 926 1054 1313 367 940 901 262
Cuts at: 6780 820
Size: 1553
Fragments arranged by size:
1553 1313 1054 940 926 901 367 262
197
SnaBI TAC'GTA
Cuts at: 493 5304 493
Size: 4811 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7513
SphI G_CATG'C
Cuts at: 1132 3141 4112 4871 1132
Size: 2009 971 759 3774
Fragments arranged by size:
3774 2009 971 759
SspI AAT'ATT
Cuts at: 5 52 2791 2950 3554 4087 4670 5437 5
Size: 47 2739 159 604 533 583 767 2081
Fragments arranged by size:
2739 2081 767 604 583 533 159 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7513
StyI C'CwwG_G
Cuts at: 513 1065 2004 2206 3165 3313 3600 4952 513
Size: 552 939 202 959 148 287 1352 3074
Fragments arranged by size:
3074 1352 959 939 552 287 202 148
SwaI ATTT'AAAT
Cuts at: 2487 2487
Size: 7513
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2066 3872 4738 5540 5557 5710 5895
Size: 1255 486 1806 866 802 17 153 185
Cuts at: 5895 6340 325
Size: 445 1498
Fragments arranged by size:
1806 1498 1255 866 802 486 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5759 7471 96
Size: 274 80 33 51 1443 3782 1712 138
Fragments arranged by size:
3782 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7473 7505 7473
Size: 32 7481
Tth111I GACn'n_nGTC
Cuts at: 1316 3014 3269 1316
Size: 1698 255 5560
Fragments arranged by size:
5560 1698 255
XbaI T'CTAG_A
Cuts at: 4849 4849
Size: 7513
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3320 3710 3811 3320
Size: 390 101 7022
Fragments arranged by size:
7022 390 101
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7513
XmaI C'CCGG_G
Cuts at: 1870 4838 1870
Size: 2968 4545
XmnI GAAnn'nnTTC
Cuts at: 5642 5642
Size: 7513
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5321 276
Size: 53 83 186 936 3787 2468
Fragments arranged by size:
3787 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI
SfoI SrfI