Restriction map for
pFN21AE3325
(Circular) (Six-base) MAPSORT of: pFN21AE3325.seq Check: 6411 from: 1 to: 7468
pFN21AE3325 7468 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3494 4488 5278 278
Size: 53 83 186 936 1958 994 790 2468
Fragments arranged by size:
2468 1958 994 936 790 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4811 1937
Size: 2874 4594
Acc65I G'GTAC_C
Cuts at: 4789 4789
Size: 7468
AclI AA'CG_TT
Cuts at: 1563 5595 5968 1563
Size: 4032 373 3063
Fragments arranged by size:
4032 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2101 2792 3869 4321 5531 6915 7183 1632
Size: 469 691 1077 452 1210 1384 268 1917
Fragments arranged by size:
1917 1384 1210 1077 691 469 452 268
AfeI AGC'GCT
Cuts at: 2984 3005 2984
Size: 21 7447
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7271
AflIII A'CryG_T
Cuts at: 1203 2382 6367 7344 1203
Size: 1179 3985 977 1327
Fragments arranged by size:
3985 1327 1179 977
AgeI A'CCGG_T
Cuts at: 6299 6299
Size: 7468
AhdI GACnn_n'nnGTC
Cuts at: 4279 6197 4279
Size: 1918 5550
AleI CACnn'nnGTG
Cuts at: 1166 2585 2726 1166
Size: 1419 141 5908
Fragments arranged by size:
5908 1419 141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7468
AlwNI CAG_nnn'CTG
Cuts at: 4140 5211 6783 4140
Size: 1071 1572 4825
Fragments arranged by size:
4825 1572 1071
ApaI G_GGCC'C
Cuts at: 2572 2572
Size: 7468
ApaLI G'TGCA_C
Cuts at: 3190 3571 5525 6681 3190
Size: 381 1954 1156 3977
Fragments arranged by size:
3977 1954 1156 381
ApoI r'AATT_y
Cuts at: 1493 4777 5003 5155 7264 1493
Size: 3284 226 152 2109 1697
Fragments arranged by size:
3284 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6022 160
Size: 872 4990 1606
Fragments arranged by size:
4990 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7468
AvaI C'yCGr_G
Cuts at: 1870 1943 2564 2614 2779 2824 3675 4793 1870
Size: 73 621 50 165 45 851 1118 4545
Fragments arranged by size:
4545 1118 851 621 165 73 50 45
BamHI G'GATC_C
Cuts at: 2487 4798 5165 2487
Size: 2311 367 4790
Fragments arranged by size:
4790 2311 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2874 3652 3658 3679 4097
Size: 325 217 627 1087 778 6 21 418
Cuts at: 4097 4414 4789 6244 618
Size: 317 375 1455 1842
Fragments arranged by size:
1842 1455 1087 778 627 418 375 325
317 217 21 6
BanII G_rGCy'C
Cuts at: 1391 2478 2513 2572 3930 4112 4787 1391
Size: 1087 35 59 1358 182 675 4072
Fragments arranged by size:
4072 1358 1087 675 182 59 35
BbeI G_GCGC'C
Cuts at: 3683 3683
Size: 7468
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4285 928
Size: 971 2386 4111
Fragments arranged by size:
4111 2386 971
BbvCI CC'TCA_GC
Cuts at: 4598 4598
Size: 7468
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3107 3141 5659 5693 1903
Size: 34 1170 34 2518 34 3678
Fragments arranged by size:
3678 2518 1170 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5360 6570 5360
Size: 1210 6258
BclI T'GATC_A
Cuts at: 1548 3294 1548
Size: 1746 5722
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4608 6079 136
Size: 107 122 71 631 605 2936 1471 1525
Fragments arranged by size:
2936 1525 1471 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3133 7463 3133
Size: 4330 3138
BlpI GC'TnA_GC
Cuts at: 2258 4885 2258
Size: 2627 4841
Bme1580I G_kGCm'C
Cuts at: 1790 2344 2468 2572 2627 2744 2877 3194 3575
Size: 554 124 104 55 117 133 317 381
Cuts at: 3575 4580 5529 6685 1790
Size: 1005 949 1156 2573
Fragments arranged by size:
2573 1156 1005 949 554 381 317 133
124 117 104 55
BmgBI CAC'GTC
Cuts at: 1318 3189 1318
Size: 1871 5597
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5199 6157 442
Size: 416 53 83 399 24 3782 958 1753
Fragments arranged by size:
3782 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7468
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2260 4131 2260
Size: 1871 5597
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2255 2285 2660 3451 3935 4721 6128
Size: 875 141 30 375 791 484 786 1407
Cuts at: 6128 1239
Size: 2579
Fragments arranged by size:
2579 1407 875 791 786 484 375 141
30
Bpu10I CC'TnA_GC
Cuts at: 2039 4598 2039
Size: 2559 4909
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2035 2918 3209 4013 4203 4262 5593 6458 6756
Size: 883 291 804 190 59 1331 865 298
Cuts at: 6756 2035
Size: 2747
Fragments arranged by size:
2747 1331 883 865 804 298 291 190
59
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4663 6131 882
Size: 617 3164 1468 2219
Fragments arranged by size:
3164 2219 1468 617
BsaAI yAC'GTr
Cuts at: 493 1204 2587 5259 493
Size: 711 1383 2672 2702
Fragments arranged by size:
2702 2672 1383 711
BsaBI GATnn'nnATC
Cuts at: 7462 7462
Size: 7468
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2607 3491 3668 3680
Size: 53 83 186 936 1074 884 177 12
Cuts at: 3680 4485 5275 5657 6292 275
Size: 805 790 382 635 1451
Fragments arranged by size:
1451 1074 936 884 805 790 635 382
186 177 83 53 12
BsaWI w'CCGG_w
Cuts at: 2085 3327 3700 5901 6299 6573 6720 7131 2085
Size: 1242 373 2201 398 274 147 411 2422
Fragments arranged by size:
2422 2201 1242 411 398 373 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3467 3497 3507 3537 3746
Size: 30 165 30 2064 30 10 30 209
Cuts at: 3746 3776 1178
Size: 30 4870
Fragments arranged by size:
4870 2064 209 165 30 30 30 30
30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2788 3321 3472 3496 3824 3830 4466
Size: 1162 440 533 151 24 328 6 636
Cuts at: 4466 1186
Size: 4188
Fragments arranged by size:
4188 1162 636 533 440 328 151 24
6
BseYI C'CCAG_C
Cuts at: 2187 2869 4647 6671 7167 2187
Size: 682 1778 2024 496 2488
Fragments arranged by size:
2488 2024 1778 682 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3377 1163
Size: 573 1641 5254
Fragments arranged by size:
5254 1641 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3967 4918 5679 6707 1540
Size: 275 186 1966 951 761 1028 2301
Fragments arranged by size:
2301 1966 1028 951 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3194 3575 3930 5529 5614 6685 3194
Size: 381 355 1599 85 1071 3977
Fragments arranged by size:
3977 1599 1071 381 355 85
BsmI GAATG_Cn'
Cuts at: 4979 5072 4979
Size: 93 7375
BsmBI CGTCTCn'nnnn_
Cuts at: 2432 3659 2432
Size: 1227 6241
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2344 2468 2478 2513 2572 2627 2744
Size: 399 554 124 10 35 59 55 117
Cuts at: 2744 2877 3194 3575 3930 4112 4580 4787 5529
Size: 133 317 381 355 182 468 207 742
Cuts at: 5529 5614 6685 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 554 468 399 381 355
317 207 182 133 124 117 85 59
55 35 10
BspEI T'CCGG_A
Cuts at: 2085 3327 7131 2085
Size: 1242 3804 2422
Fragments arranged by size:
3804 2422 1242
BspHI T'CATG_A
Cuts at: 5357 7087 5357
Size: 1730 5738
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3399 3606 4823 844
Size: 2555 207 1217 3489
Fragments arranged by size:
3489 2555 1217 207
BsrBI CCG'CTC
Cuts at: 1394 1823 3015 5355 1394
Size: 429 1192 2340 3507
Fragments arranged by size:
3507 2340 1192 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5963 6137 65
Size: 1183 4715 174 1396
Fragments arranged by size:
4715 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2121 3655 6112 6299 1700
Size: 110 311 1534 2457 187 2869
Fragments arranged by size:
2869 2457 1534 311 187 110
BsrGI T'GTAC_A
Cuts at: 96 2577 96
Size: 2481 4987
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7468
BssSI C'ACGA_G
Cuts at: 2858 5528 6540 2858
Size: 2670 1012 3786
Fragments arranged by size:
3786 2670 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7468
BstBI TT'CG_AA
Cuts at: 6272 6272
Size: 7468
BstEII G'GTnAC_C
Cuts at: 1184 3703 1184
Size: 2519 4949
BstXI CCAn_nnnn'nTGG
Cuts at: 4690 4690
Size: 7468
BstYI r'GATC_y
Cuts at: 1972 2487 2937 3120 3133 3310 4798 5165 5550
Size: 515 450 183 13 177 1488 367 385
Cuts at: 5550 5567 7019 7105 7117 7463 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1488 1452 515 450 385 367 346
183 177 86 17 13 12
Bsu36I CC'TnA_GG
Cuts at: 2369 2369
Size: 7468
BtgI C'CryG_G
Cuts at: 513 1065 2483 2601 3961 513
Size: 552 1418 118 1360 4020
Fragments arranged by size:
4020 1418 1360 552 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2652 487
Size: 683 56 1426 5303
Fragments arranged by size:
5303 1426 683 56
BtsI GCAGTG_nn'
Cuts at: 4984 5778 5798 4984
Size: 794 20 6654
Fragments arranged by size:
6654 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7468
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7433
DraI TTT'AAA
Cuts at: 4772 4772
Size: 7468
DraIII CAC_nnn'GTG
Cuts at: 2484 2881 2484
Size: 397 7071
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6475 809
Size: 507 5159 1802
Fragments arranged by size:
5159 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2251 3964 3984
Size: 54 1425 50 275 18 421 1713 20
Cuts at: 3984 4122 4915 5804 7433 8
Size: 138 793 889 1629 43
Fragments arranged by size:
1713 1629 1425 889 793 421 275 138
54 50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 3964 4915 1537
Size: 275 2152 951 4090
Fragments arranged by size:
4090 2152 951 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2909 3768 3789 4340 4646 4659 5398 1353
Size: 1556 859 21 551 306 13 739 3423
Fragments arranged by size:
3423 1556 859 739 551 306 21 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4418 6055 6429 6575 387
Size: 1064 138 2829 1637 374 146 1280
Fragments arranged by size:
2829 1637 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3928 3928
Size: 7468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2101 2114 2255 2285 2660 2792 3451
Size: 393 469 13 141 30 375 132 659
Cuts at: 3451 3869 3935 4321 4721 5531 6128 6915 7183
Size: 418 66 386 400 810 597 787 268
Cuts at: 7183 1239
Size: 1524
Fragments arranged by size:
1524 810 787 659 597 469 418 400
393 386 375 268 141 132 66 30
13
EcoNI CCTnn'n_nnAGG
Cuts at: 2368 4142 4268 2368
Size: 1774 126 5568
Fragments arranged by size:
5568 1774 126
EcoO109I rG'GnC_Cy
Cuts at: 2230 2269 2518 2568 2569 2784 4273 2230
Size: 39 249 50 1 215 1489 5425
Fragments arranged by size:
5425 1489 249 215 50 39 1
EcoRI G'AATT_C
Cuts at: 4777 5155 4777
Size: 378 7090
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 3230 3961 4309 4483 2167
Size: 1063 731 348 174 5152
Fragments arranged by size:
5152 1063 731 348 174
FspI TGC'GCA
Cuts at: 2222 4385 5974 2222
Size: 2163 1589 3716
Fragments arranged by size:
3716 2163 1589
HaeII r_GCGC'y
Cuts at: 1678 2986 3007 3683 6615 1678
Size: 1308 21 676 2932 2531
Fragments arranged by size:
2932 2531 1308 676 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3697 3729 3746 3778 4140
Size: 32 135 32 2125 32 17 32 362
Cuts at: 4140 4172 4265 4297 6109 6141 6183 6215 1373
Size: 32 93 32 1812 32 42 32 2626
Fragments arranged by size:
2626 2125 1812 362 135 93 42 32
32 32 32 32 32 32 32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 4198 4812 5058 669
Size: 988 281 2260 614 246 3079
Fragments arranged by size:
3079 2260 988 614 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7468
HpaI GTT'AAC
Cuts at: 5058 5058
Size: 7468
KasI G'GCGC_C
Cuts at: 3679 3679
Size: 7468
KpnI G_GTAC'C
Cuts at: 4793 4793
Size: 7468
MfeI C'AATT_G
Cuts at: 5067 5067
Size: 7468
MluI A'CGCG_T
Cuts at: 7344 7344
Size: 7468
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2049 2085 3744 6582 6766 1305
Size: 744 36 1659 2838 184 2007
Fragments arranged by size:
2838 2007 1659 744 184 36
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3986 4124 10
Size: 54 1425 343 2154 138 3354
Fragments arranged by size:
3354 2154 1425 343 138 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 2726 4583 4694 5426 5785 5944
Size: 648 1419 141 1857 111 732 359 159
Cuts at: 5944 518
Size: 2042
Fragments arranged by size:
2042 1857 1419 732 648 359 159 141
111
MspA1I CmG'CkG
Cuts at: 1606 2128 2257 2680 2869 2958 2992 3913 3969
Size: 522 129 423 189 89 34 921 56
Cuts at: 3969 4435 4884 5561 6709 6954 1606
Size: 466 449 677 1148 245 2120
Fragments arranged by size:
2120 1148 921 677 522 466 449 423
245 189 129 89 56 34
NaeI GCC'GGC
Cuts at: 2123 2123
Size: 7468
NarI GG'CG_CC
Cuts at: 3680 3680
Size: 7468
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6916
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7468
NgoMIV G'CCGG_C
Cuts at: 2121 2121
Size: 7468
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7468
NotI GC'GGCC_GC
Cuts at: 4915 4915
Size: 7468
NspI r_CATG'y
Cuts at: 1132 1229 2386 4699 4826 6371 1132
Size: 97 1157 2313 127 1545 2229
Fragments arranged by size:
2313 2229 1545 1157 127 97
PasI CC'CwG_GG
Cuts at: 1348 2514 1348
Size: 1166 6302
PciI A'CATG_T
Cuts at: 2382 6367 2382
Size: 3985 3483
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2424 2484 3174 4530 1108
Size: 1316 60 690 1356 4046
Fragments arranged by size:
4046 1356 1316 690 60
PfoI T'CCnGG_A
Cuts at: 2232 2232
Size: 7468
PmeI GTTT'AAAC
Cuts at: 4772 4772
Size: 7468
PmlI CAC'GTG
Cuts at: 2587 2587
Size: 7468
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 2989 5537 5569 7050 7082 2957
Size: 32 2548 32 1481 32 3343
Fragments arranged by size:
3343 2548 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2230 2269 2518 2784 4273 2230
Size: 39 249 266 1489 5425
Fragments arranged by size:
5425 1489 266 249 39
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7468
PsiI TTA'TAA
Cuts at: 5038 5038
Size: 7468
PspOMI G'GGCC_C
Cuts at: 2568 2568
Size: 7468
PstI C_TGCA'G
Cuts at: 830 4359 4762 4820 830
Size: 3529 403 58 3478
Fragments arranged by size:
3529 3478 403 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7468
PvuII CAG'CTG
Cuts at: 2128 2257 2869 2958 3913 4435 2128
Size: 129 612 89 955 522 5161
Fragments arranged by size:
5161 955 612 522 129 89
RsrII CG'GwC_CG
Cuts at: 2689 2689
Size: 7468
SacI G_AGCT'C
Cuts at: 3930 3930
Size: 7468
SalI G'TCGA_C
Cuts at: 1936 4810 1936
Size: 2874 4594
SanDI GG'GwC_CC
Cuts at: 2518 2784 2518
Size: 266 7202
SapI GCTCTTCn'nnn_
Cuts at: 2909 2909
Size: 7468
SbfI CC_TGCA'GG
Cuts at: 4820 4820
Size: 7468
ScaI AGT'ACT
Cuts at: 2114 3173 5716 7428 2114
Size: 1059 2543 1712 2154
Fragments arranged by size:
2543 2154 1712 1059
SfcI C'TryA_G
Cuts at: 826 1045 4057 4355 4758 4816 5951 6632 6823
Size: 219 3012 298 403 58 1135 681 191
Cuts at: 6823 826
Size: 1471
Fragments arranged by size:
3012 1471 1135 681 403 298 219 191
58
SfoI GGC'GCC
Cuts at: 3681 3681
Size: 7468
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7468
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7468
SmaI CCC'GGG
Cuts at: 1872 2566 2616 2781 2826 3677 4795 1872
Size: 694 50 165 45 851 1118 4545
Fragments arranged by size:
4545 1118 851 694 165 50 45
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2014 2933 3224 4028 4218 4277
Size: 197 926 71 919 291 804 190 59
Cuts at: 4277 5572 6473 6735 820
Size: 1295 901 262 1553
Fragments arranged by size:
1553 1295 926 919 901 804 291 262
197 190 71 59
SnaBI TAC'GTA
Cuts at: 493 5259 493
Size: 4766 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7468
SphI G_CATG'C
Cuts at: 1132 4826 1132
Size: 3694 3774
SspI AAT'ATT
Cuts at: 5 52 5392 5
Size: 47 5340 2081
Fragments arranged by size:
5340 2081 47
StuI AGG'CCT
Cuts at: 1517 4034 1517
Size: 2517 4951
StyI C'CwwG_G
Cuts at: 513 1065 4254 4495 4907 513
Size: 552 3189 241 412 3074
Fragments arranged by size:
3189 3074 552 412 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2510 2597 4085 5495 5512 5665 5850
Size: 1255 930 87 1488 1410 17 153 185
Cuts at: 5850 6295 325
Size: 445 1498
Fragments arranged by size:
1498 1488 1410 1255 930 445 185 153
87 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 2577 3171
Size: 274 80 33 51 1443 135 465 594
Cuts at: 3171 5714 7426 96
Size: 2543 1712 138
Fragments arranged by size:
2543 1712 1443 594 465 274 138 135
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2035 2067 3146 3178 3507 3539 7428 7460 2035
Size: 32 1079 32 329 32 3889 32 2043
Fragments arranged by size:
3889 2043 1079 329 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3334 1316
Size: 2018 5450
XbaI T'CTAG_A
Cuts at: 4804 4804
Size: 7468
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4186 4186
Size: 7468
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7468
XmaI C'CCGG_G
Cuts at: 1870 2564 2614 2779 2824 3675 4793 1870
Size: 694 50 165 45 851 1118 4545
Fragments arranged by size:
4545 1118 851 694 165 50 45
XmnI GAAnn'nnTTC
Cuts at: 2114 3590 4292 5597 2114
Size: 1476 702 1305 3985
Fragments arranged by size:
3985 1476 1305 702
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3492 4486 5276 276
Size: 53 83 186 936 1958 994 790 2468
Fragments arranged by size:
2468 1958 994 936 790 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI
SacII SexAI SfiI SrfI SwaI