Restriction map for
pFN21AE3321
(Circular) (Six-base) MAPSORT of: pFN21AE3321.seq Check: 7597 from: 1 to: 7417
pFN21AE3321 7417 bp
With 182 enzymes: SgfI *
March 28, 2012 12:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5227 278
Size: 53 83 186 936 3691 2468
Fragments arranged by size:
3691 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4760 1937
Size: 2823 4594
Acc65I G'GTAC_C
Cuts at: 2137 2979 4738 2137
Size: 842 1759 4816
Fragments arranged by size:
4816 1759 842
AclI AA'CG_TT
Cuts at: 1563 5544 5917 1563
Size: 3981 373 3063
Fragments arranged by size:
3981 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2144 3839 5480 6864 7132 1632
Size: 512 1695 1641 1384 268 1917
Fragments arranged by size:
1917 1695 1641 1384 512 268
AfeI AGC'GCT
Cuts at: 2559 2559
Size: 7417
AflII C'TTAA_G
Cuts at: 820 1017 3154 820
Size: 197 2137 5083
Fragments arranged by size:
5083 2137 197
AflIII A'CryG_T
Cuts at: 1203 3576 3953 4714 6316 7293 1203
Size: 2373 377 761 1602 977 1327
Fragments arranged by size:
2373 1602 1327 977 761 377
AgeI A'CCGG_T
Cuts at: 6248 6248
Size: 7417
AhdI GACnn_n'nnGTC
Cuts at: 6146 6146
Size: 7417
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7417
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7417
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2490 2522 2490
Size: 32 7385
AlwNI CAG_nnn'CTG
Cuts at: 3815 5160 6732 3815
Size: 1345 1572 4500
Fragments arranged by size:
4500 1572 1345
ApaLI G'TGCA_C
Cuts at: 2086 5474 6630 2086
Size: 3388 1156 2873
Fragments arranged by size:
3388 2873 1156
ApoI r'AATT_y
Cuts at: 1493 2374 2446 2550 2986 3603 4358 4726 4952
Size: 881 72 104 436 617 755 368 226
Cuts at: 4952 5104 7213 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 881 755 617 436 368 226
152 104 72
AseI AT'TA_AT
Cuts at: 160 1032 5971 160
Size: 872 4939 1606
Fragments arranged by size:
4939 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7417
AvaI C'yCGr_G
Cuts at: 1870 1943 4742 1870
Size: 73 2799 4545
Fragments arranged by size:
4545 2799 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2121 2154 3192 3225 2121
Size: 33 1038 33 6313
Fragments arranged by size:
6313 1038 33 33
BamHI G'GATC_C
Cuts at: 4697 4747 5114 4697
Size: 50 367 7000
Fragments arranged by size:
7000 367 50
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2137 2979 4664 4738 6193
Size: 325 217 627 350 842 1685 74 1455
Cuts at: 6193 618
Size: 1842
Fragments arranged by size:
1842 1685 1455 842 627 350 325 217
74
BanII G_rGCy'C
Cuts at: 1391 4736 1391
Size: 3345 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3739 4350 928
Size: 971 1840 611 3995
Fragments arranged by size:
3995 1840 971 611
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2867 2901 5608 5642 1903
Size: 34 930 34 2707 34 3678
Fragments arranged by size:
3678 2707 930 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4148 5309 6519 4148
Size: 1161 1210 5046
Fragments arranged by size:
5046 1210 1161
BclI T'GATC_A
Cuts at: 1548 4125 1548
Size: 2577 4840
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6028 136
Size: 107 122 71 631 605 4356 1525
Fragments arranged by size:
4356 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2461 7412 2461
Size: 4951 2466
BlpI GC'TnA_GC
Cuts at: 4834 4834
Size: 7417
Bme1580I G_kGCm'C
Cuts at: 1790 2090 4105 5478 6634 1790
Size: 300 2015 1373 1156 2573
Fragments arranged by size:
2573 2015 1373 1156 300
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7417
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3731 4058 5148
Size: 416 53 83 399 24 2314 327 1090
Cuts at: 5148 6106 442
Size: 958 1753
Fragments arranged by size:
2314 1753 1090 958 416 399 327 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2306 2628 4207 6077 1239
Size: 1055 12 322 1579 1870 2579
Fragments arranged by size:
2579 1870 1579 1055 322 12
Bpu10I CC'TnA_GC
Cuts at: 4011 4011
Size: 7417
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3611 4580 5542 6407 6705 3611
Size: 969 962 865 298 4323
Fragments arranged by size:
4323 969 962 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3986 6080 882
Size: 617 2487 2094 2219
Fragments arranged by size:
2487 2219 2094 617
BsaAI yAC'GTr
Cuts at: 493 1204 3954 5208 493
Size: 711 2750 1254 2702
Fragments arranged by size:
2750 2702 1254 711
BsaBI GATnn'nnATC
Cuts at: 4151 7411 4151
Size: 3260 4157
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5224 5606 6241 275
Size: 53 83 186 936 3691 382 635 1451
Fragments arranged by size:
3691 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5850 6248 6522 6669 7080 5850
Size: 398 274 147 411 6187
Fragments arranged by size:
6187 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3447 3477 1178
Size: 30 165 30 2044 30 5118
Fragments arranged by size:
5118 2044 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4577 1186
Size: 3391 4026
BseYI C'CCAG_C
Cuts at: 2701 2708 6620 7116 2701
Size: 7 3912 496 3002
Fragments arranged by size:
3912 3002 496 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2353 1163
Size: 573 617 6227
Fragments arranged by size:
6227 617 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4867 5628 6656 1540
Size: 275 186 2866 761 1028 2301
Fragments arranged by size:
2866 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2090 3081 5478 5563 6634 2090
Size: 991 2397 85 1071 2873
Fragments arranged by size:
2873 2397 1071 991 85
BsmI GAATG_Cn'
Cuts at: 2751 4928 5021 2751
Size: 2177 93 5147
Fragments arranged by size:
5147 2177 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 3081 4105 4736 5478 5563 6634
Size: 399 300 991 1024 631 742 85 1071
Cuts at: 6634 1391
Size: 2174
Fragments arranged by size:
2174 1071 1024 991 742 631 399 300
85
BspEI T'CCGG_A
Cuts at: 7080 7080
Size: 7417
BspHI T'CATG_A
Cuts at: 4122 5306 7036 4122
Size: 1184 1730 4503
Fragments arranged by size:
4503 1730 1184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3417 4772 844
Size: 2573 1355 3489
Fragments arranged by size:
3489 2573 1355
BsrBI CCG'CTC
Cuts at: 1394 1823 5304 1394
Size: 429 3481 3507
Fragments arranged by size:
3507 3481 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5912 6086 65
Size: 1183 4664 174 1396
Fragments arranged by size:
4664 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6061 6248 1700
Size: 110 4251 187 2869
Fragments arranged by size:
4251 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4711 96
Size: 4615 2802
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7417
BssSI C'ACGA_G
Cuts at: 5477 6489 5477
Size: 1012 6405
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7417
BstBI TT'CG_AA
Cuts at: 3381 6221 3381
Size: 2840 4577
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7417
BstXI CCAn_nnnn'nTGG
Cuts at: 4549 4549
Size: 7417
BstYI r'GATC_y
Cuts at: 1972 2461 3538 3732 4697 4747 5114 5499 5516
Size: 489 1077 194 965 50 367 385 17
Cuts at: 5516 6968 7054 7066 7412 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1077 965 489 385 367 346
194 86 50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6865
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6678
Fragments arranged by size:
6678 683 56
BtsI GCAGTG_nn'
Cuts at: 4624 4933 5727 5747 4624
Size: 309 794 20 6294
Fragments arranged by size:
6294 794 309 20
ClaI AT'CG_AT
Cuts at: 1554 3777 1554
Size: 2223 5194
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4013 4048 529
Size: 35 3449 35 3898
Fragments arranged by size:
3898 3449 35 35
DraI TTT'AAA
Cuts at: 2601 3798 4618 4721 2601
Size: 1197 820 103 5297
Fragments arranged by size:
5297 1197 820 103
DraIII CAC_nnn'GTG
Cuts at: 2085 2085
Size: 7417
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6424 809
Size: 507 5108 1802
Fragments arranged by size:
5108 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4640 4864 5753
Size: 54 1425 50 275 18 2810 224 889
Cuts at: 5753 7382 8
Size: 1629 43
Fragments arranged by size:
2810 1629 1425 889 275 224 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4864 1537
Size: 275 3052 4090
Fragments arranged by size:
4090 3052 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3522 4333 4600 5347 1353
Size: 2169 811 267 747 3423
Fragments arranged by size:
3423 2169 811 747 267
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6004 6378 6524 387
Size: 1064 138 4415 374 146 1280
Fragments arranged by size:
4415 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2294 2306 2628 3839 4207 5480
Size: 393 512 150 12 322 1211 368 1273
Cuts at: 5480 6077 6864 7132 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1273 1211 787 597 512 393 368
322 268 150 12
EcoO109I rG'GnC_Cy
Cuts at: 2041 2705 2041
Size: 664 6753
EcoRI G'AATT_C
Cuts at: 4726 5104 4726
Size: 378 7039
EcoRV GAT'ATC
Cuts at: 2581 4194 2581
Size: 1613 5804
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4709 4709
Size: 7417
FspI TGC'GCA
Cuts at: 5923 5923
Size: 7417
HaeII r_GCGC'y
Cuts at: 1678 2561 6564 1678
Size: 883 4003 2531
Fragments arranged by size:
4003 2531 883
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2603 2635 3269 3301 3447
Size: 32 135 32 1031 32 634 32 146
Cuts at: 3447 3479 4070 4102 6058 6090 6132 6164 1373
Size: 32 591 32 1956 32 42 32 2626
Fragments arranged by size:
2626 1956 1031 634 591 146 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2130 4417 4761 5007 669
Size: 988 281 192 2287 344 246 3079
Fragments arranged by size:
3079 2287 988 344 281 246 192
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7417
HpaI GTT'AAC
Cuts at: 4417 5007 4417
Size: 590 6827
KpnI G_GTAC'C
Cuts at: 2141 2983 4742 2141
Size: 842 1759 4816
Fragments arranged by size:
4816 1759 842
MfeI C'AATT_G
Cuts at: 2661 4410 5016 2661
Size: 1749 606 5062
Fragments arranged by size:
5062 1749 606
MluI A'CGCG_T
Cuts at: 7293 7293
Size: 7417
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2424 3189 3561 3741 6531 6715 1305
Size: 1119 765 372 180 2790 184 2007
Fragments arranged by size:
2790 2007 1119 765 372 184 180
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4642 10
Size: 54 1425 343 2810 2785
Fragments arranged by size:
2810 2785 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2165 4053 5375 5734 5893 518
Size: 648 999 1888 1322 359 159 2042
Fragments arranged by size:
2042 1888 1322 999 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2512 2701 2782 3415 4833 5510 6658 6903
Size: 906 189 81 633 1418 677 1148 245
Cuts at: 6903 1606
Size: 2120
Fragments arranged by size:
2120 1418 1148 906 677 633 245 189
81
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6865
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7417
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7417
NotI GC'GGCC_GC
Cuts at: 4864 4864
Size: 7417
NspI r_CATG'y
Cuts at: 1132 1229 3580 4718 4775 6320 1132
Size: 97 2351 1138 57 1545 2229
Fragments arranged by size:
2351 2229 1545 1138 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7417
PciI A'CATG_T
Cuts at: 3576 4714 6316 3576
Size: 1138 1602 4677
Fragments arranged by size:
4677 1602 1138
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7417
PmeI GTTT'AAAC
Cuts at: 4721 4721
Size: 7417
PmlI CAC'GTG
Cuts at: 3954 3954
Size: 7417
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2376 2408 5486 5518 6999 7031 2376
Size: 32 3078 32 1481 32 2762
Fragments arranged by size:
3078 2762 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2705 2705
Size: 7417
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7417
PsiI TTA'TAA
Cuts at: 4454 4578 4987 4454
Size: 124 409 6884
Fragments arranged by size:
6884 409 124
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4209 4241 4209
Size: 32 7385
PstI C_TGCA'G
Cuts at: 830 2117 3414 4769 830
Size: 1287 1297 1355 3478
Fragments arranged by size:
3478 1355 1297 1287
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7417
PvuII CAG'CTG
Cuts at: 2512 2701 2782 3415 2512
Size: 189 81 633 6514
Fragments arranged by size:
6514 633 189 81
SalI G'TCGA_C
Cuts at: 1936 4759 1936
Size: 2823 4594
SanDI GG'GwC_CC
Cuts at: 2705 2705
Size: 7417
SbfI CC_TGCA'GG
Cuts at: 4769 4769
Size: 7417
ScaI AGT'ACT
Cuts at: 3309 5665 7377 3309
Size: 2356 1712 3349
Fragments arranged by size:
3349 2356 1712
SfcI C'TryA_G
Cuts at: 826 1045 2113 3410 4765 5900 6581 6772 826
Size: 219 1068 1297 1355 1135 681 191 1471
Fragments arranged by size:
1471 1355 1297 1135 1068 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7417
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7417
SmaI CCC'GGG
Cuts at: 1872 4744 1872
Size: 2872 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3154 3590 4559 5521 6422 6684
Size: 197 926 1211 436 969 962 901 262
Cuts at: 6684 820
Size: 1553
Fragments arranged by size:
1553 1211 969 962 926 901 436 262
197
SnaBI TAC'GTA
Cuts at: 493 5208 493
Size: 4715 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7417
SphI G_CATG'C
Cuts at: 1132 4775 1132
Size: 3643 3774
SspI AAT'ATT
Cuts at: 5 52 2909 3376 5341 5
Size: 47 2857 467 1965 2081
Fragments arranged by size:
2857 2081 1965 467 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7417
StyI C'CwwG_G
Cuts at: 513 1065 3435 4856 513
Size: 552 2370 1421 3074
Fragments arranged by size:
3074 2370 1421 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5444 5461 5614 5799 6244 325
Size: 1255 3864 17 153 185 445 1498
Fragments arranged by size:
3864 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3307 4162 4216
Size: 274 80 33 51 1443 1330 855 54
Cuts at: 4216 4711 5663 7375 96
Size: 495 952 1712 138
Fragments arranged by size:
1712 1443 1330 952 855 495 274 138
80 54 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2050 2082 7377 7409 2050
Size: 32 5295 32 2058
Fragments arranged by size:
5295 2058 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7417
XbaI T'CTAG_A
Cuts at: 3367 4753 3367
Size: 1386 6031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2269 2269
Size: 7417
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7417
XmaI C'CCGG_G
Cuts at: 1870 4742 1870
Size: 2872 4545
XmnI GAAnn'nnTTC
Cuts at: 3890 5546 3890
Size: 1656 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5225 276
Size: 53 83 186 936 3691 2468
Fragments arranged by size:
3691 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PspOMI PspXI RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI