Restriction map for
pFN21AE3319
(Circular) (Six-base) MAPSORT of: pFN21AE3319.seq Check: 6227 from: 1 to: 7372
pFN21AE3319 7372 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3037 4406 4597 3037
Size: 1369 191 5812
Fragments arranged by size:
5812 1369 191
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5182 278
Size: 53 83 186 936 3646 2468
Fragments arranged by size:
3646 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4715 1937
Size: 2778 4594
Acc65I G'GTAC_C
Cuts at: 3214 4693 3214
Size: 1479 5893
AclI AA'CG_TT
Cuts at: 1563 5499 5872 1563
Size: 3936 373 3063
Fragments arranged by size:
3936 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2117 2378 2486 2790 3656 3794 4079 4118
Size: 485 261 108 304 866 138 285 39
Cuts at: 4118 4441 5435 6819 7087 1632
Size: 323 994 1384 268 1917
Fragments arranged by size:
1917 1384 994 866 485 323 304 285
268 261 138 108 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7175
AflIII A'CryG_T
Cuts at: 1203 2391 6271 7248 1203
Size: 1188 3880 977 1327
Fragments arranged by size:
3880 1327 1188 977
AgeI A'CCGG_T
Cuts at: 6203 6203
Size: 7372
AhdI GACnn_n'nnGTC
Cuts at: 4648 6101 4648
Size: 1453 5919
AleI CACnn'nnGTG
Cuts at: 1166 2964 1166
Size: 1798 5574
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7372
AlwNI CAG_nnn'CTG
Cuts at: 2170 2173 2449 2531 2689 2722 2878 3226 5115
Size: 3 276 82 158 33 156 348 1889
Cuts at: 5115 6687 2170
Size: 1572 2855
Fragments arranged by size:
2855 1889 1572 348 276 158 156 82
33 3
ApaLI G'TGCA_C
Cuts at: 5429 6585 5429
Size: 1156 6216
ApoI r'AATT_y
Cuts at: 1493 4681 4907 5059 7168 1493
Size: 3188 226 152 2109 1697
Fragments arranged by size:
3188 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5926 160
Size: 872 4894 1606
Fragments arranged by size:
4894 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7372
AvaI C'yCGr_G
Cuts at: 1870 1943 2766 3406 3974 4187 4409 4697 1870
Size: 73 823 640 568 213 222 288 4545
Fragments arranged by size:
4545 823 640 568 288 222 213 73
AvrII C'CTAG_G
Cuts at: 3614 3614
Size: 7372
BamHI G'GATC_C
Cuts at: 3831 4702 5069 3831
Size: 871 367 6134
Fragments arranged by size:
6134 871 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2319 2405 2591 2785
Size: 325 217 627 230 302 86 186 194
Cuts at: 2785 3214 3369 3858 4166 4693 6148 618
Size: 429 155 489 308 527 1455 1842
Fragments arranged by size:
1842 1455 627 527 489 429 325 308
302 230 217 194 186 155 86
BanII G_rGCy'C
Cuts at: 1391 2317 3100 3981 4691 1391
Size: 926 783 881 710 4072
Fragments arranged by size:
4072 926 881 783 710
BbeI G_GCGC'C
Cuts at: 2021 2595 3373 2021
Size: 574 778 6020
Fragments arranged by size:
6020 778 574
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3509 3961 928
Size: 971 1610 452 4339
Fragments arranged by size:
4339 1610 971 452
BbvCI CC'TCA_GC
Cuts at: 2586 2652 3206 2586
Size: 66 554 6752
Fragments arranged by size:
6752 554 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5563 5597 1903
Size: 34 3626 34 3678
Fragments arranged by size:
3678 3626 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2786 5264 6474 2786
Size: 2478 1210 3684
Fragments arranged by size:
3684 2478 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7372
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2154 2862 2915
Size: 107 122 71 631 605 482 708 53
Cuts at: 2915 5983 136
Size: 3068 1525
Fragments arranged by size:
3068 1525 708 631 605 482 122 107
71 53
BglII A'GATC_T
Cuts at: 7367 7367
Size: 7372
BlpI GC'TnA_GC
Cuts at: 2268 3688 3791 4789 2268
Size: 1420 103 998 4851
Fragments arranged by size:
4851 1420 998 103
Bme1580I G_kGCm'C
Cuts at: 1790 3861 4277 4397 5433 6589 1790
Size: 2071 416 120 1036 1156 2573
Fragments arranged by size:
2573 2071 1156 1036 416 120
BmgBI CAC'GTC
Cuts at: 1318 3626 1318
Size: 2308 5064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 5103 6061
Size: 416 53 83 399 24 2060 1626 958
Cuts at: 6061 442
Size: 1753
Fragments arranged by size:
2060 1753 1626 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 2522 2744 3110 3128 3857 3883 4370
Size: 977 306 222 366 18 729 26 487
Cuts at: 4370 6032 1239
Size: 1662 2579
Fragments arranged by size:
2579 1662 977 729 487 366 306 222
26 18
Bpu10I CC'TnA_GC
Cuts at: 2309 2532 2586 2652 3206 2309
Size: 223 54 66 554 6475
Fragments arranged by size:
6475 554 223 66 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3188 4385 5497 6362 6660 3188
Size: 1197 1112 865 298 3900
Fragments arranged by size:
3900 1197 1112 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6035 882
Size: 617 4536 2219
Fragments arranged by size:
4536 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2394 3997 5163 493
Size: 711 1190 1603 1166 2702
Fragments arranged by size:
2702 1603 1190 1166 711
BsaBI GATnn'nnATC
Cuts at: 2489 3953 7366 2489
Size: 1464 3413 2495
Fragments arranged by size:
3413 2495 1464
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2592 3370 4329
Size: 53 83 186 936 485 574 778 959
Cuts at: 4329 5179 5561 6196 275
Size: 850 382 635 1451
Fragments arranged by size:
1451 959 936 850 778 635 574 485
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2334 4146 5805 6203 6477 6624 7035 2334
Size: 1812 1659 398 274 147 411 2671
Fragments arranged by size:
2671 1812 1659 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2679 2709 1178
Size: 30 165 30 1276 30 5841
Fragments arranged by size:
5841 1276 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2561 3593 4373 4448 4595 1186
Size: 838 537 1032 780 75 147 3963
Fragments arranged by size:
3963 1032 838 780 537 147 75
BseYI C'CCAG_C
Cuts at: 2263 2439 2664 2799 2902 3039 3697 4237 6575
Size: 176 225 135 103 137 658 540 2338
Cuts at: 6575 7071 2263
Size: 496 2564
Fragments arranged by size:
2564 2338 658 540 496 225 176 137
135 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2183 2210 2549 3835 4575 1163
Size: 573 447 27 339 1286 740 3960
Fragments arranged by size:
3960 1286 740 573 447 339 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2294 2910 4822 5583 6611 1540
Size: 275 186 293 616 1912 761 1028 2301
Fragments arranged by size:
2301 1912 1028 761 616 293 275 186
BsiHKAI G_wGCw'C
Cuts at: 3413 3792 5433 5518 6589 3413
Size: 379 1641 85 1071 4196
Fragments arranged by size:
4196 1641 1071 379 85
BsmI GAATG_Cn'
Cuts at: 4883 4976 4883
Size: 93 7279
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2317 3100 3413 3792 3861 3981 4277
Size: 399 527 783 313 379 69 120 296
Cuts at: 4277 4397 4691 5433 5518 6589 1391
Size: 120 294 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 527 399 379 313
296 294 120 120 85 69
BspEI T'CCGG_A
Cuts at: 2334 7035 2334
Size: 4701 2671
BspHI T'CATG_A
Cuts at: 3378 3945 5261 6991 3378
Size: 567 1316 1730 3759
Fragments arranged by size:
3759 1730 1316 567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2130 2839 3037 3393 4406 4545 4597 4727
Size: 1286 709 198 356 1013 139 52 130
Cuts at: 4727 844
Size: 3489
Fragments arranged by size:
3489 1286 1013 709 356 198 139 130
52
BsrBI CCG'CTC
Cuts at: 1394 1823 5259 1394
Size: 429 3436 3507
Fragments arranged by size:
3507 3436 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5867 6041 65
Size: 1183 4619 174 1396
Fragments arranged by size:
4619 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6016 6203 1700
Size: 110 4206 187 2869
Fragments arranged by size:
4206 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7372
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7372
BssSI C'ACGA_G
Cuts at: 3959 4031 5432 6444 3959
Size: 72 1401 1012 4887
Fragments arranged by size:
4887 1401 1012 72
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2170 2173 2449 2563 3226 3787 4480 794
Size: 1376 3 276 114 663 561 693 3686
Fragments arranged by size:
3686 1376 693 663 561 276 114 3
BstBI TT'CG_AA
Cuts at: 6176 6176
Size: 7372
BstEII G'GTnAC_C
Cuts at: 1184 3241 3938 1184
Size: 2057 697 4618
Fragments arranged by size:
4618 2057 697
BstXI CCAn_nnnn'nTGG
Cuts at: 2602 2602
Size: 7372
BstYI r'GATC_y
Cuts at: 1972 3831 3954 4702 5069 5454 5471 6923 7009
Size: 1859 123 748 367 385 17 1452 86
Cuts at: 7009 7021 7367 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1859 1452 748 385 367 346 123
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2281 3329 2281
Size: 1048 6324
BtgI C'CryG_G
Cuts at: 513 1065 2004 2021 2294 513
Size: 552 939 17 273 5591
Fragments arranged by size:
5591 939 552 273 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6633
Fragments arranged by size:
6633 683 56
BtsI GCAGTG_nn'
Cuts at: 2843 3452 4608 4888 5682 5702 2843
Size: 609 1156 280 794 20 4513
Fragments arranged by size:
4513 1156 794 609 280 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7372
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7337
DraI TTT'AAA
Cuts at: 4676 4676
Size: 7372
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4316 6379 809
Size: 507 3000 2063 1802
Fragments arranged by size:
3000 2063 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2291 2907
Size: 54 1425 50 275 18 303 158 616
Cuts at: 2907 2916 2968 3051 4819 5708 7337 8
Size: 9 52 83 1768 889 1629 43
Fragments arranged by size:
1768 1629 1425 889 616 303 275 158
83 54 52 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2291 2907 4819 1537
Size: 275 479 616 1912 4090
Fragments arranged by size:
4090 1912 616 479 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3513 4293 5302 1353
Size: 2160 780 1009 3423
Fragments arranged by size:
3423 2160 1009 780
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2063 2558 2843 5959 6333 6479
Size: 1064 138 474 495 285 3116 374 146
Cuts at: 6479 387
Size: 1280
Fragments arranged by size:
3116 1280 1064 495 474 374 285 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2216 2378 2486 2522 2744 2790
Size: 393 485 99 162 108 36 222 46
Cuts at: 2790 3110 3128 3656 3794 3857 3883 4079 4118
Size: 320 18 528 138 63 26 196 39
Cuts at: 4118 4370 4441 5435 6032 6819 7087 1239
Size: 252 71 994 597 787 268 1524
Fragments arranged by size:
1524 994 787 597 528 485 393 320
268 252 222 196 162 138 108 99
71 63 46 39 36 26 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3560 3560
Size: 7372
EcoO109I rG'GnC_Cy
Cuts at: 2285 2514 2528 3721 4054 2285
Size: 229 14 1193 333 5603
Fragments arranged by size:
5603 1193 333 229 14
EcoRI G'AATT_C
Cuts at: 4681 5059 4681
Size: 378 6994
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3526 3526
Size: 7372
FspI TGC'GCA
Cuts at: 2753 5878 2753
Size: 3125 4247
HaeII r_GCGC'y
Cuts at: 1678 2021 2595 2899 3373 4408 6519 1678
Size: 343 574 304 474 1035 2111 2531
Fragments arranged by size:
2531 2111 1035 574 474 343 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3557 3589 4634 4666 6013
Size: 32 135 32 1985 32 1045 32 1347
Cuts at: 6013 6045 6087 6119 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1985 1347 1045 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4321 4716 4962 669
Size: 988 281 2383 395 246 3079
Fragments arranged by size:
3079 2383 988 395 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7372
HpaI GTT'AAC
Cuts at: 4962 4962
Size: 7372
KasI G'GCGC_C
Cuts at: 2017 2591 3369 2017
Size: 574 778 6020
Fragments arranged by size:
6020 778 574
KpnI G_GTAC'C
Cuts at: 3218 4697 3218
Size: 1479 5893
MfeI C'AATT_G
Cuts at: 4971 4971
Size: 7372
MluI A'CGCG_T
Cuts at: 7248 7248
Size: 7372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 4044 6486 6670 1305
Size: 903 1836 2442 184 2007
Fragments arranged by size:
2442 2007 1836 903 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 2918 10
Size: 54 1425 343 303 783 4464
Fragments arranged by size:
4464 1425 783 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2964 3963 5330 5689 5848 518
Size: 648 1798 999 1367 359 159 2042
Fragments arranged by size:
2042 1798 1367 999 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2170 2203 2267 2344 2435 2464 2579
Size: 417 147 33 64 77 91 29 115
Cuts at: 2579 2719 2906 3684 4788 5465 6613 6858 1606
Size: 140 187 778 1104 677 1148 245 2120
Fragments arranged by size:
2120 1148 1104 778 677 417 245 187
147 140 115 91 77 64 33 29
NarI GG'CG_CC
Cuts at: 2018 2592 3370 2018
Size: 574 778 6020
Fragments arranged by size:
6020 778 574
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5881
Fragments arranged by size:
5881 939 552
NdeI CA'TA_TG
Cuts at: 387 3606 387
Size: 3219 4153
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7372
NotI GC'GGCC_GC
Cuts at: 2907 4819 2907
Size: 1912 5460
NspI r_CATG'y
Cuts at: 1132 1229 2351 4730 6275 1132
Size: 97 1122 2379 1545 2229
Fragments arranged by size:
2379 2229 1545 1122 97
PasI CC'CwG_GG
Cuts at: 1348 2252 4087 4283 1348
Size: 904 1835 196 4437
Fragments arranged by size:
4437 1835 904 196
PciI A'CATG_T
Cuts at: 6271 6271
Size: 7372
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4470 1108
Size: 3362 4010
PfoI T'CCnGG_A
Cuts at: 3177 3834 4632 4662 3177
Size: 657 798 30 5887
Fragments arranged by size:
5887 798 657 30
PmeI GTTT'AAAC
Cuts at: 4676 4676
Size: 7372
PmlI CAC'GTG
Cuts at: 2394 2394
Size: 7372
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 4541 4573 5441 5473 6954 6986 4262
Size: 32 247 32 868 32 1481 32 4648
Fragments arranged by size:
4648 1481 868 247 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2285 2514 3721 4054 2285
Size: 229 1207 333 5603
Fragments arranged by size:
5603 1207 333 229
PshAI GACnn'nnGTC
Cuts at: 1613 3077 1613
Size: 1464 5908
PsiI TTA'TAA
Cuts at: 4942 4942
Size: 7372
PstI C_TGCA'G
Cuts at: 830 2850 3285 3401 4023 4724 830
Size: 2020 435 116 622 701 3478
Fragments arranged by size:
3478 2020 701 622 435 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7372
PvuII CAG'CTG
Cuts at: 2170 2203 2267 2344 2435 2464 2579 2719 2170
Size: 33 64 77 91 29 115 140 6823
Fragments arranged by size:
6823 140 115 91 77 64 33 29
SacII CC_GC'GG
Cuts at: 2024 2024
Size: 7372
SalI G'TCGA_C
Cuts at: 1936 4714 1936
Size: 2778 4594
SanDI GG'GwC_CC
Cuts at: 2514 2514
Size: 7372
SbfI CC_TGCA'GG
Cuts at: 4724 4724
Size: 7372
ScaI AGT'ACT
Cuts at: 5620 7332 5620
Size: 1712 5660
SfcI C'TryA_G
Cuts at: 826 1045 2552 2669 2846 3281 3397 4019 4720
Size: 219 1507 117 177 435 116 622 701
Cuts at: 4720 5855 6536 6727 826
Size: 1135 681 191 1471
Fragments arranged by size:
1507 1471 1135 701 681 622 435 219
191 177 117 116
SfiI GGCCn_nnn'nGGCC
Cuts at: 2915 2915
Size: 7372
SfoI GGC'GCC
Cuts at: 2019 2593 3371 2019
Size: 574 778 6020
Fragments arranged by size:
6020 778 574
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7372
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7372
SmaI CCC'GGG
Cuts at: 1872 4699 1872
Size: 2827 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3167 4364 5476 6377 6639 820
Size: 197 926 1224 1197 1112 901 262 1553
Fragments arranged by size:
1553 1224 1197 1112 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5163 493
Size: 4670 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7372
SphI G_CATG'C
Cuts at: 1132 4730 1132
Size: 3598 3774
SspI AAT'ATT
Cuts at: 5 52 5296 5
Size: 47 5244 2081
Fragments arranged by size:
5244 2081 47
StuI AGG'CCT
Cuts at: 1517 3464 1517
Size: 1947 5425
StyI C'CwwG_G
Cuts at: 513 1065 2004 2412 2709 2949 3510 3614 4811
Size: 552 939 408 297 240 561 104 1197
Cuts at: 4811 513
Size: 3074
Fragments arranged by size:
3074 1197 939 561 552 408 297 240
104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3503 3550 4229 4256 5399 5416 5569
Size: 1255 1923 47 679 27 1143 17 153
Cuts at: 5569 5754 6199 325
Size: 185 445 1498
Fragments arranged by size:
1923 1498 1255 1143 679 445 185 153
47 27 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3816 5618 7330
Size: 274 80 33 51 1443 1839 1802 1712
Cuts at: 7330 96
Size: 138
Fragments arranged by size:
1839 1802 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2835 2867 3185 3217 3583 3610 3615 3642 7332
Size: 32 318 32 366 27 5 27 3690
Cuts at: 7332 7364 2835
Size: 32 2843
Fragments arranged by size:
3690 2843 366 318 32 32 32 27
27 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7372
XbaI T'CTAG_A
Cuts at: 4708 4708
Size: 7372
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2191 2992 2191
Size: 801 6571
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7372
XmaI C'CCGG_G
Cuts at: 1870 4697 1870
Size: 2827 4545
XmnI GAAnn'nnTTC
Cuts at: 5501 5501
Size: 7372
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5180 276
Size: 53 83 186 936 3646 2468
Fragments arranged by size:
3646 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacI SapI SexAI SrfI SwaI