Restriction map for
pFN21AE3317
(Circular) (Six-base) MAPSORT of: pFN21AE3317.seq Check: 8589 from: 1 to: 7342
pFN21AE3317 7342 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5152 278
Size: 53 83 186 936 3616 2468
Fragments arranged by size:
3616 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2322 4281 4685 1937
Size: 385 1959 404 4594
Fragments arranged by size:
4594 1959 404 385
Acc65I G'GTAC_C
Cuts at: 4663 4663
Size: 7342
AclI AA'CG_TT
Cuts at: 1563 3938 5469 5842 1563
Size: 2375 1531 373 3063
Fragments arranged by size:
3063 2375 1531 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2439 3327 4492 5405 6789 7057 1632
Size: 807 888 1165 913 1384 268 1917
Fragments arranged by size:
1917 1384 1165 913 888 807 268
AflII C'TTAA_G
Cuts at: 820 1017 3476 3649 820
Size: 197 2459 173 4513
Fragments arranged by size:
4513 2459 197 173
AflIII A'CryG_T
Cuts at: 1203 6241 7218 1203
Size: 5038 977 1327
Fragments arranged by size:
5038 1327 977
AgeI A'CCGG_T
Cuts at: 6173 6173
Size: 7342
AhdI GACnn_n'nnGTC
Cuts at: 6071 6071
Size: 7342
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7342
AlwNI CAG_nnn'CTG
Cuts at: 4618 5085 6657 4618
Size: 467 1572 5303
Fragments arranged by size:
5303 1572 467
ApaI G_GGCC'C
Cuts at: 3011 4267 3011
Size: 1256 6086
ApaLI G'TGCA_C
Cuts at: 3488 5399 6555 3488
Size: 1911 1156 4275
Fragments arranged by size:
4275 1911 1156
ApoI r'AATT_y
Cuts at: 1493 2105 3340 3729 4215 4313 4326 4651 4877
Size: 612 1235 389 486 98 13 325 226
Cuts at: 4877 5029 7138 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1235 612 486 389 325 226
152 98 13
AseI AT'TA_AT
Cuts at: 160 1032 2562 3990 5896 160
Size: 872 1530 1428 1906 1606
Fragments arranged by size:
1906 1606 1530 1428 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7342
AvaI C'yCGr_G
Cuts at: 1870 1943 4667 1870
Size: 73 2724 4545
Fragments arranged by size:
4545 2724 73
BamHI G'GATC_C
Cuts at: 2008 4672 5039 2008
Size: 2664 367 4311
Fragments arranged by size:
4311 2664 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2135 4663 6118 618
Size: 325 217 627 348 2528 1455 1842
Fragments arranged by size:
2528 1842 1455 627 348 325 217
BanII G_rGCy'C
Cuts at: 1391 3011 4267 4661 1391
Size: 1620 1256 394 4072
Fragments arranged by size:
4072 1620 1256 394
BbeI G_GCGC'C
Cuts at: 2139 2139
Size: 7342
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3624 928
Size: 971 1725 4646
Fragments arranged by size:
4646 1725 971
BbvCI CC'TCA_GC
Cuts at: 3859 3859
Size: 7342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3863 3897 5533 5567 1903
Size: 34 1926 34 1636 34 3678
Fragments arranged by size:
3678 1926 1636 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5234 6444 5234
Size: 1210 6132
BclI T'GATC_A
Cuts at: 1548 2211 1548
Size: 663 6679
BfrBI ATG'CAT
Cuts at: 3995 3995
Size: 7342
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5953 136
Size: 107 122 71 631 605 4281 1525
Fragments arranged by size:
4281 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7337 7337
Size: 7342
BlpI GC'TnA_GC
Cuts at: 4611 4759 4611
Size: 148 7194
Bme1580I G_kGCm'C
Cuts at: 1790 3011 3136 3492 4267 4294 5403 6559 1790
Size: 1221 125 356 775 27 1109 1156 2573
Fragments arranged by size:
2573 1221 1156 1109 775 356 125 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7342
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4531 5073 6031
Size: 416 53 83 399 24 3114 542 958
Cuts at: 6031 442
Size: 1753
Fragments arranged by size:
3114 1753 958 542 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7342
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2058 2861 3119 3343 3944 6002 1239
Size: 819 803 258 224 601 2058 2579
Fragments arranged by size:
2579 2058 819 803 601 258 224
Bpu10I CC'TnA_GC
Cuts at: 3859 4245 3859
Size: 386 6956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2727 5467 6332 6630 2727
Size: 2740 865 298 3439
Fragments arranged by size:
3439 2740 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6005 882
Size: 617 4506 2219
Fragments arranged by size:
4506 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5133 493
Size: 711 3929 2702
Fragments arranged by size:
3929 2702 711
BsaBI GATnn'nnATC
Cuts at: 2573 7336 2573
Size: 4763 2579
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 5149 5531 6166
Size: 53 83 186 936 603 3013 382 635
Cuts at: 6166 275
Size: 1451
Fragments arranged by size:
3013 1451 936 635 603 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2479 5775 6173 6447 6594 7005 2479
Size: 3296 398 274 147 411 2816
Fragments arranged by size:
3296 2816 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7117
Fragments arranged by size:
7117 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2055 2072 3909 1186
Size: 869 17 1837 4619
Fragments arranged by size:
4619 1837 869 17
BseYI C'CCAG_C
Cuts at: 4560 6545 7041 4560
Size: 1985 496 4861
Fragments arranged by size:
4861 1985 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3488 4455 1163
Size: 573 1752 967 4050
Fragments arranged by size:
4050 1752 967 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4792 5553 6581 1540
Size: 275 186 2791 761 1028 2301
Fragments arranged by size:
2791 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3315 3492 5403 5488 6559 3315
Size: 177 1911 85 1071 4098
Fragments arranged by size:
4098 1911 1071 177 85
BsmI GAATG_Cn'
Cuts at: 2850 2957 3034 4853 4946 2850
Size: 107 77 1819 93 5246
Fragments arranged by size:
5246 1819 107 93 77
BsmBI CGTCTCn'nnnn_
Cuts at: 3065 3065
Size: 7342
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3011 3136 3315 3492 4267 4294 4661
Size: 399 1221 125 179 177 775 27 367
Cuts at: 4661 5403 5488 6559 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1221 1071 775 742 399 367 179
177 125 85 27
BspEI T'CCGG_A
Cuts at: 7005 7005
Size: 7342
BspHI T'CATG_A
Cuts at: 5231 6961 5231
Size: 1730 5612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4697 844
Size: 3853 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5229 1394
Size: 429 3406 3507
Fragments arranged by size:
3507 3406 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4050 5837 6011 65
Size: 1183 2802 1787 174 1396
Fragments arranged by size:
2802 1787 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5986 6173 1700
Size: 110 4176 187 2869
Fragments arranged by size:
4176 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3886 96
Size: 3790 3552
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7342
BssSI C'ACGA_G
Cuts at: 5402 6414 5402
Size: 1012 6330
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3056 794
Size: 2262 5080
BstBI TT'CG_AA
Cuts at: 3970 6146 3970
Size: 2176 5166
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7342
BstYI r'GATC_y
Cuts at: 1972 2008 3094 3820 4109 4672 5039 5424 5441
Size: 36 1086 726 289 563 367 385 17
Cuts at: 5441 6893 6979 6991 7337 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1086 726 563 385 367 346
289 86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 4080 4267 513
Size: 552 939 12 2064 187 3588
Fragments arranged by size:
3588 2064 939 552 187 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6603
Fragments arranged by size:
6603 683 56
BtsI GCAGTG_nn'
Cuts at: 2085 2300 3136 4858 5652 5672 2085
Size: 215 836 1722 794 20 3755
Fragments arranged by size:
3755 1722 836 794 215 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7342
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7307
DraI TTT'AAA
Cuts at: 3333 4646 3333
Size: 1313 6029
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6349 809
Size: 507 5033 1802
Fragments arranged by size:
5033 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2895 4394 4789
Size: 54 1425 50 275 18 1065 1499 395
Cuts at: 4789 5678 7307 8
Size: 889 1629 43
Fragments arranged by size:
1629 1499 1425 1065 889 395 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4789 1537
Size: 275 2977 4090
Fragments arranged by size:
4090 2977 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2388 3303 4169 4180 4511 5272 1353
Size: 1035 915 866 11 331 761 3423
Fragments arranged by size:
3423 1035 915 866 761 331 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5929 6303 6449 387
Size: 1064 138 4340 374 146 1280
Fragments arranged by size:
4340 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2439 2861 3119 3327 3343 3944
Size: 393 426 381 422 258 208 16 601
Cuts at: 3944 4492 5405 6002 6789 7057 1239
Size: 548 913 597 787 268 1524
Fragments arranged by size:
1524 913 787 601 597 548 426 422
393 381 268 258 208 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2040 2040
Size: 7342
EcoO109I rG'GnC_Cy
Cuts at: 3472 3914 4263 3472
Size: 442 349 6551
Fragments arranged by size:
6551 442 349
EcoRI G'AATT_C
Cuts at: 2105 4215 4326 4651 5029 2105
Size: 2110 111 325 378 4418
Fragments arranged by size:
4418 2110 378 325 111
EcoRV GAT'ATC
Cuts at: 2575 3303 4387 2575
Size: 728 1084 5530
Fragments arranged by size:
5530 1084 728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3332 3842 3332
Size: 510 6832
FspI TGC'GCA
Cuts at: 3049 5848 3049
Size: 2799 4543
HaeII r_GCGC'y
Cuts at: 1678 2139 6489 1678
Size: 461 4350 2531
Fragments arranged by size:
4350 2531 461
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2442 2474 4123
Size: 32 135 32 773 32 65 32 1649
Cuts at: 4123 4155 5983 6015 6057 6089 1373
Size: 32 1828 32 42 32 2626
Fragments arranged by size:
2626 1828 1649 773 135 65 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3355 3500 4686 4932 669
Size: 988 281 1417 145 1186 246 3079
Fragments arranged by size:
3079 1417 1186 988 281 246 145
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7342
HpaI GTT'AAC
Cuts at: 4932 4932
Size: 7342
KasI G'GCGC_C
Cuts at: 2135 2135
Size: 7342
KpnI G_GTAC'C
Cuts at: 4667 4667
Size: 7342
MfeI C'AATT_G
Cuts at: 2673 4941 2673
Size: 2268 5074
MluI A'CGCG_T
Cuts at: 7218 7218
Size: 7342
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2859 3005 6456 6640 1305
Size: 1554 146 3451 184 2007
Fragments arranged by size:
3451 2007 1554 184 146
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2897 4396 10
Size: 54 1425 343 1065 1499 2956
Fragments arranged by size:
2956 1499 1425 1065 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2376 2451 3990 5300 5659 5818 518
Size: 648 1210 75 1539 1310 359 159 2042
Fragments arranged by size:
2042 1539 1310 1210 648 359 159 75
MspA1I CmG'CkG
Cuts at: 1606 2018 4758 5435 6583 6828 1606
Size: 412 2740 677 1148 245 2120
Fragments arranged by size:
2740 2120 1148 677 412 245
NarI GG'CG_CC
Cuts at: 2136 2136
Size: 7342
NcoI C'CATG_G
Cuts at: 513 1065 2004 4080 4267 513
Size: 552 939 2076 187 3588
Fragments arranged by size:
3588 2076 939 552 187
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7342
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7342
NotI GC'GGCC_GC
Cuts at: 4789 4789
Size: 7342
NsiI A_TGCA'T
Cuts at: 3997 3997
Size: 7342
NspI r_CATG'y
Cuts at: 1132 1229 4700 6245 1132
Size: 97 3471 1545 2229
Fragments arranged by size:
3471 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7342
PciI A'CATG_T
Cuts at: 6241 6241
Size: 7342
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7342
PfoI T'CCnGG_A
Cuts at: 3724 3724
Size: 7342
PmeI GTTT'AAAC
Cuts at: 4646 4646
Size: 7342
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5411 5443 6924 6956 5411
Size: 32 1481 32 5797
Fragments arranged by size:
5797 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3472 3914 3472
Size: 442 6900
PshAI GACnn'nnGTC
Cuts at: 1613 3249 3360 1613
Size: 1636 111 5595
Fragments arranged by size:
5595 1636 111
PsiI TTA'TAA
Cuts at: 4912 4912
Size: 7342
PspOMI G'GGCC_C
Cuts at: 3007 4263 3007
Size: 1256 6086
PstI C_TGCA'G
Cuts at: 830 2307 4536 4694 830
Size: 1477 2229 158 3478
Fragments arranged by size:
3478 2229 1477 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7342
SacII CC_GC'GG
Cuts at: 2019 2019
Size: 7342
SalI G'TCGA_C
Cuts at: 1936 4684 1936
Size: 2748 4594
SapI GCTCTTCn'nnn_
Cuts at: 3303 3303
Size: 7342
SbfI CC_TGCA'GG
Cuts at: 4694 4694
Size: 7342
ScaI AGT'ACT
Cuts at: 5590 7302 5590
Size: 1712 5630
SfcI C'TryA_G
Cuts at: 826 1045 2303 2766 4063 4532 4690 5825 6506
Size: 219 1258 463 1297 469 158 1135 681
Cuts at: 6506 6697 826
Size: 191 1471
Fragments arranged by size:
1471 1297 1258 1135 681 469 463 219
191 158
SfoI GGC'GCC
Cuts at: 2137 2137
Size: 7342
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7342
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7342
SmaI CCC'GGG
Cuts at: 1872 4669 1872
Size: 2797 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2742 3476 3649 5446 6347 6609
Size: 197 926 799 734 173 1797 901 262
Cuts at: 6609 820
Size: 1553
Fragments arranged by size:
1797 1553 926 901 799 734 262 197
173
SnaBI TAC'GTA
Cuts at: 493 5133 493
Size: 4640 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7342
SphI G_CATG'C
Cuts at: 1132 4700 1132
Size: 3568 3774
SspI AAT'ATT
Cuts at: 5 52 3697 3814 4372 5266 5
Size: 47 3645 117 558 894 2081
Fragments arranged by size:
3645 2081 894 558 117 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7342
StyI C'CwwG_G
Cuts at: 513 1065 2004 3316 4080 4267 4391 4781 513
Size: 552 939 1312 764 187 124 390 3074
Fragments arranged by size:
3074 1312 939 764 552 390 187 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5369 5386 5539 5724 6169 325
Size: 1255 3789 17 153 185 445 1498
Fragments arranged by size:
3789 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3886 5588 7300
Size: 274 80 33 51 1443 1909 1702 1712
Cuts at: 7300 96
Size: 138
Fragments arranged by size:
1909 1712 1702 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2835 2867 4040 4072 7302 7334 2835
Size: 32 1173 32 3230 32 2843
Fragments arranged by size:
3230 2843 1173 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7342
XbaI T'CTAG_A
Cuts at: 2434 4678 2434
Size: 2244 5098
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7342
XmaI C'CCGG_G
Cuts at: 1870 4667 1870
Size: 2797 4545
XmnI GAAnn'nnTTC
Cuts at: 3080 5471 3080
Size: 2391 4951
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5150 276
Size: 53 83 186 936 3616 2468
Fragments arranged by size:
3616 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI NaeI
NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII
SacI SanDI SexAI SfiI SrfI SwaI XcmI