Restriction map for
pFN21AE3313
(Circular) (Six-base) MAPSORT of: pFN21AE3313.seq Check: 7023 from: 1 to: 7246
pFN21AE3313 7246 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5056 278
Size: 53 83 186 936 3520 2468
Fragments arranged by size:
3520 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2826 4589 1937
Size: 889 1763 4594
Fragments arranged by size:
4594 1763 889
Acc65I G'GTAC_C
Cuts at: 4567 4567
Size: 7246
AclI AA'CG_TT
Cuts at: 1563 2691 5373 5746 1563
Size: 1128 2682 373 3063
Fragments arranged by size:
3063 2682 1128 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2982 3078 5309 6693 6961 1632
Size: 1350 96 2231 1384 268 1917
Fragments arranged by size:
2231 1917 1384 1350 268 96
AflII C'TTAA_G
Cuts at: 820 1017 2572 820
Size: 197 1555 5494
Fragments arranged by size:
5494 1555 197
AflIII A'CryG_T
Cuts at: 1203 3402 3534 6145 7122 1203
Size: 2199 132 2611 977 1327
Fragments arranged by size:
2611 2199 1327 977 132
AgeI A'CCGG_T
Cuts at: 6077 6077
Size: 7246
AhdI GACnn_n'nnGTC
Cuts at: 5975 5975
Size: 7246
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7246
AlwNI CAG_nnn'CTG
Cuts at: 2960 3686 4325 4989 6561 2960
Size: 726 639 664 1572 3645
Fragments arranged by size:
3645 1572 726 664 639
ApaLI G'TGCA_C
Cuts at: 5303 6459 5303
Size: 1156 6090
ApoI r'AATT_y
Cuts at: 1493 2327 2474 2831 2855 3779 3917 3948 4343
Size: 834 147 357 24 924 138 31 395
Cuts at: 4343 4422 4555 4781 4933 7042 1493
Size: 79 133 226 152 2109 1697
Fragments arranged by size:
2109 1697 924 834 395 357 226 152
147 138 133 79 31 24
AseI AT'TA_AT
Cuts at: 160 1032 2336 2519 3597 5800 160
Size: 872 1304 183 1078 2203 1606
Fragments arranged by size:
2203 1606 1304 1078 872 183
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7246
AvaI C'yCGr_G
Cuts at: 1870 1943 4571 1870
Size: 73 2628 4545
Fragments arranged by size:
4545 2628 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2340 2373 2340
Size: 33 7213
BamHI G'GATC_C
Cuts at: 3829 4576 4943 3829
Size: 747 367 6132
Fragments arranged by size:
6132 747 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4567 6022 618
Size: 325 217 627 2780 1455 1842
Fragments arranged by size:
2780 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4565 1391
Size: 3174 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2255 928
Size: 971 356 5919
Fragments arranged by size:
5919 971 356
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5437 5471 1903
Size: 34 3500 34 3678
Fragments arranged by size:
3678 3500 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5138 6348 5138
Size: 1210 6036
BclI T'GATC_A
Cuts at: 1548 3397 1548
Size: 1849 5397
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3683 5857 136
Size: 107 122 71 631 605 2011 2174 1525
Fragments arranged by size:
2174 2011 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3889 7241 3889
Size: 3352 3894
BlpI GC'TnA_GC
Cuts at: 4663 4663
Size: 7246
Bme1580I G_kGCm'C
Cuts at: 1790 5307 6463 1790
Size: 3517 1156 2573
Fragments arranged by size:
3517 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7246
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4447 4977 5935
Size: 416 53 83 399 24 3030 530 958
Cuts at: 5935 442
Size: 1753
Fragments arranged by size:
3030 1753 958 530 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2919 1056
Size: 1863 5383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2101 5906 1239
Size: 862 3805 2579
Fragments arranged by size:
3805 2579 862
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3135 5371 6236 6534 3135
Size: 2236 865 298 3847
Fragments arranged by size:
3847 2236 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2277 5909 882
Size: 617 778 3632 2219
Fragments arranged by size:
3632 2219 778 617
BsaAI yAC'GTr
Cuts at: 493 1204 5037 493
Size: 711 3833 2702
Fragments arranged by size:
3833 2702 711
BsaBI GATnn'nnATC
Cuts at: 7240 7240
Size: 7246
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5053 5435 6070 275
Size: 53 83 186 936 3520 382 635 1451
Fragments arranged by size:
3520 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5679 6077 6351 6498 6909 5679
Size: 398 274 147 411 6016
Fragments arranged by size:
6016 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 1178
Size: 30 165 30 741 30 6250
Fragments arranged by size:
6250 741 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 3279 1186
Size: 979 1114 5153
Fragments arranged by size:
5153 1114 979
BseYI C'CCAG_C
Cuts at: 6449 6945 6449
Size: 496 6750
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6673
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4696 5457 6485 1540
Size: 275 186 2695 761 1028 2301
Fragments arranged by size:
2695 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5307 5392 6463 5307
Size: 85 1071 6090
Fragments arranged by size:
6090 1071 85
BsmI GAATG_Cn'
Cuts at: 4025 4757 4850 4025
Size: 732 93 6421
Fragments arranged by size:
6421 732 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4565 5307 5392 6463 1391
Size: 399 2775 742 85 1071 2174
Fragments arranged by size:
2775 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6909 6909
Size: 7246
BspHI T'CATG_A
Cuts at: 4048 5135 6865 4048
Size: 1087 1730 4429
Fragments arranged by size:
4429 1730 1087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4601 844
Size: 3757 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5133 1394
Size: 429 3310 3507
Fragments arranged by size:
3507 3310 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2949 3051 5741 5915 65
Size: 1183 1701 102 2690 174 1396
Fragments arranged by size:
2690 1701 1396 1183 174 102
BsrFI r'CCGG_y
Cuts at: 1700 1810 5890 6077 1700
Size: 110 4080 187 2869
Fragments arranged by size:
4080 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2141 96
Size: 2045 5201
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7246
BssSI C'ACGA_G
Cuts at: 5306 6318 5306
Size: 1012 6234
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7246
BstBI TT'CG_AA
Cuts at: 2719 6050 2719
Size: 3331 3915
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7246
BstXI CCAn_nnnn'nTGG
Cuts at: 2079 2079
Size: 7246
BstYI r'GATC_y
Cuts at: 1972 3496 3705 3829 3889 4576 4943 5328 5345
Size: 1524 209 124 60 687 367 385 17
Cuts at: 5345 6797 6883 6895 7241 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1524 1452 687 385 367 346 209
124 86 60 17 12
BstZ17I GTA'TAC
Cuts at: 2827 2827
Size: 7246
BtgI C'CryG_G
Cuts at: 513 1065 4040 513
Size: 552 2975 3719
Fragments arranged by size:
3719 2975 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6507
Fragments arranged by size:
6507 683 56
BtsI GCAGTG_nn'
Cuts at: 3039 4762 5556 5576 3039
Size: 1723 794 20 4709
Fragments arranged by size:
4709 1723 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7246
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7211
DraI TTT'AAA
Cuts at: 4117 4550 4117
Size: 433 6813
DraIII CAC_nnn'GTG
Cuts at: 3341 3341
Size: 7246
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6253 809
Size: 507 4937 1802
Fragments arranged by size:
4937 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3864 4693 5582
Size: 54 1425 50 275 18 2034 829 889
Cuts at: 5582 7211 8
Size: 1629 43
Fragments arranged by size:
2034 1629 1425 889 829 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4693 1537
Size: 275 2881 4090
Fragments arranged by size:
4090 2881 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2310 4233 5176 1353
Size: 957 1923 943 3423
Fragments arranged by size:
3423 1923 957 943
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5833 6207 6353 387
Size: 1064 138 4244 374 146 1280
Fragments arranged by size:
4244 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2101 2982 3078 5309 5906 6693 6961
Size: 393 469 881 96 2231 597 787 268
Cuts at: 6961 1239
Size: 1524
Fragments arranged by size:
2231 1524 881 787 597 469 393 268
96
EcoO109I rG'GnC_Cy
Cuts at: 2945 2957 3007 2945
Size: 12 50 7184
Fragments arranged by size:
7184 50 12
EcoRI G'AATT_C
Cuts at: 4555 4933 4555
Size: 378 6868
EcoRV GAT'ATC
Cuts at: 2732 3312 2732
Size: 580 6666
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2207 3108 4353 2207
Size: 901 1245 5100
Fragments arranged by size:
5100 1245 901
FspI TGC'GCA
Cuts at: 5752 5752
Size: 7246
HaeII r_GCGC'y
Cuts at: 1678 6393 1678
Size: 4715 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3608 3640 5887 5919 5961
Size: 32 135 32 2036 32 2247 32 42
Cuts at: 5961 5993 1373
Size: 32 2626
Fragments arranged by size:
2626 2247 2036 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2599 2689 4064 4482 4590 4836
Size: 988 281 661 90 1375 418 108 246
Cuts at: 4836 669
Size: 3079
Fragments arranged by size:
3079 1375 988 661 418 281 246 108
90
HindIII A'AGCT_T
Cuts at: 748 2234 748
Size: 1486 5760
HpaI GTT'AAC
Cuts at: 4836 4836
Size: 7246
KpnI G_GTAC'C
Cuts at: 4571 4571
Size: 7246
MfeI C'AATT_G
Cuts at: 4147 4845 4147
Size: 698 6548
MluI A'CGCG_T
Cuts at: 7122 7122
Size: 7246
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4282 6360 6544 1305
Size: 2977 2078 184 2007
Fragments arranged by size:
2977 2078 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3866 10
Size: 54 1425 343 2034 3390
Fragments arranged by size:
3390 2034 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3114 5204 5563 5722 518
Size: 648 1948 2090 359 159 2042
Fragments arranged by size:
2090 2042 1948 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4662 5339 6487 6732 1606
Size: 3056 677 1148 245 2120
Fragments arranged by size:
3056 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 4040 513
Size: 552 2975 3719
Fragments arranged by size:
3719 2975 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7246
NheI G'CTAG_C
Cuts at: 1052 2915 1052
Size: 1863 5383
NotI GC'GGCC_GC
Cuts at: 4693 4693
Size: 7246
NspI r_CATG'y
Cuts at: 1132 1229 3406 4604 6149 1132
Size: 97 2177 1198 1545 2229
Fragments arranged by size:
2229 2177 1545 1198 97
PasI CC'CwG_GG
Cuts at: 1348 4278 1348
Size: 2930 4316
PciI A'CATG_T
Cuts at: 3402 6145 3402
Size: 2743 4503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7246
PfoI T'CCnGG_A
Cuts at: 2078 2078
Size: 7246
PmeI GTTT'AAAC
Cuts at: 4550 4550
Size: 7246
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5315 5347 6828 6860 5315
Size: 32 1481 32 5701
Fragments arranged by size:
5701 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2945 2957 3007 2945
Size: 12 50 7184
Fragments arranged by size:
7184 50 12
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7246
PsiI TTA'TAA
Cuts at: 2864 4816 2864
Size: 1952 5294
PstI C_TGCA'G
Cuts at: 830 4598 830
Size: 3768 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7246
SalI G'TCGA_C
Cuts at: 1936 4588 1936
Size: 2652 4594
SanDI GG'GwC_CC
Cuts at: 2945 2945
Size: 7246
SbfI CC_TGCA'GG
Cuts at: 4598 4598
Size: 7246
ScaI AGT'ACT
Cuts at: 3640 3655 5494 7206 3640
Size: 15 1839 1712 3680
Fragments arranged by size:
3680 1839 1712 15
SfcI C'TryA_G
Cuts at: 826 1045 4594 5729 6410 6601 826
Size: 219 3549 1135 681 191 1471
Fragments arranged by size:
3549 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7246
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7246
SmaI CCC'GGG
Cuts at: 1872 4573 1872
Size: 2701 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2572 3150 5350 6251 6513 820
Size: 197 926 629 578 2200 901 262 1553
Fragments arranged by size:
2200 1553 926 901 629 578 262 197
SnaBI TAC'GTA
Cuts at: 493 5037 493
Size: 4544 2702
SpeI A'CTAG_T
Cuts at: 152 2419 152
Size: 2267 4979
SphI G_CATG'C
Cuts at: 1132 4604 1132
Size: 3472 3774
SspI AAT'ATT
Cuts at: 5 52 3878 5170 5
Size: 47 3826 1292 2081
Fragments arranged by size:
3826 2081 1292 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7246
StyI C'CwwG_G
Cuts at: 513 1065 4040 4685 513
Size: 552 2975 645 3074
Fragments arranged by size:
3074 2975 645 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5273 5290 5443 5628 6073 325
Size: 1255 3693 17 153 185 445 1498
Fragments arranged by size:
3693 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2141 3638 3653
Size: 274 80 33 51 1443 164 1497 15
Cuts at: 3653 5492 7204 96
Size: 1839 1712 138
Fragments arranged by size:
1839 1712 1497 1443 274 164 138 80
51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2007 2039 3780 3812 7206 7238 2007
Size: 32 1741 32 3394 32 2015
Fragments arranged by size:
3394 2015 1741 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7246
XbaI T'CTAG_A
Cuts at: 4582 4582
Size: 7246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4275 4275
Size: 7246
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7246
XmaI C'CCGG_G
Cuts at: 1870 4571 1870
Size: 2701 4545
XmnI GAAnn'nnTTC
Cuts at: 2892 3879 5375 2892
Size: 987 1496 4763
Fragments arranged by size:
4763 1496 987
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5054 276
Size: 53 83 186 936 3520 2468
Fragments arranged by size:
3520 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI