Restriction map for
pFN21AE3310
(Circular) (Six-base) MAPSORT of: pFN21AE3310.seq Check: 1606 from: 1 to: 7162
pFN21AE3310 7162 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3681 4972 278
Size: 53 83 186 936 2145 1291 2468
Fragments arranged by size:
2468 2145 1291 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4363 4505 1937
Size: 2426 142 4594
Fragments arranged by size:
4594 2426 142
Acc65I G'GTAC_C
Cuts at: 4483 4483
Size: 7162
AclI AA'CG_TT
Cuts at: 1563 5289 5662 1563
Size: 3726 373 3063
Fragments arranged by size:
3726 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3938 3980 5225 6609 6877 1632
Size: 2306 42 1245 1384 268 1917
Fragments arranged by size:
2306 1917 1384 1245 268 42
AflII C'TTAA_G
Cuts at: 820 1017 3797 820
Size: 197 2780 4185
Fragments arranged by size:
4185 2780 197
AflIII A'CryG_T
Cuts at: 1203 6061 7038 1203
Size: 4858 977 1327
Fragments arranged by size:
4858 1327 977
AgeI A'CCGG_T
Cuts at: 5993 5993
Size: 7162
AhdI GACnn_n'nnGTC
Cuts at: 3394 3580 5891 3394
Size: 186 2311 4665
Fragments arranged by size:
4665 2311 186
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4469 1184
Size: 3285 3877
AlwNI CAG_nnn'CTG
Cuts at: 2129 2210 2954 3542 4905 6477 2129
Size: 81 744 588 1363 1572 2814
Fragments arranged by size:
2814 1572 1363 744 588 81
ApaLI G'TGCA_C
Cuts at: 5219 6375 5219
Size: 1156 6006
ApoI r'AATT_y
Cuts at: 1493 3464 4471 4697 4849 6958 1493
Size: 1971 1007 226 152 2109 1697
Fragments arranged by size:
2109 1971 1697 1007 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4046 5716 160
Size: 872 3014 1670 1606
Fragments arranged by size:
3014 1670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7162
AvaI C'yCGr_G
Cuts at: 1870 1943 2895 4487 1870
Size: 73 952 1592 4545
Fragments arranged by size:
4545 1592 952 73
BamHI G'GATC_C
Cuts at: 2842 4492 4859 2842
Size: 1650 367 5145
Fragments arranged by size:
5145 1650 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2580 2697 2775 3653 4483
Size: 325 217 627 793 117 78 878 830
Cuts at: 4483 5938 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 878 830 793 627 325 217
117 78
BanII G_rGCy'C
Cuts at: 1391 2226 2496 4481 1391
Size: 835 270 1985 4072
Fragments arranged by size:
4072 1985 835 270
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3311 3729 928
Size: 971 1412 418 4361
Fragments arranged by size:
4361 1412 971 418
BbvCI CC'TCA_GC
Cuts at: 3717 3717
Size: 7162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5353 5387 1903
Size: 34 3416 34 3678
Fragments arranged by size:
3678 3416 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3019 5054 6264 3019
Size: 2035 1210 3917
Fragments arranged by size:
3917 2035 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7162
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5773 136
Size: 107 122 71 631 605 4101 1525
Fragments arranged by size:
4101 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3487 4126 4412 7157 3487
Size: 639 286 2745 3492
Fragments arranged by size:
3492 2745 639 286
BlpI GC'TnA_GC
Cuts at: 4579 4579
Size: 7162
Bme1580I G_kGCm'C
Cuts at: 1790 4255 5223 6379 1790
Size: 2465 968 1156 2573
Fragments arranged by size:
2573 2465 1156 968
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7162
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2051 4893 5851
Size: 416 53 83 399 24 634 2842 958
Cuts at: 5851 442
Size: 1753
Fragments arranged by size:
2842 1753 958 634 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7162
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 7162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2480 4254 5822 1239
Size: 1241 1774 1568 2579
Fragments arranged by size:
2579 1774 1568 1241
Bpu10I CC'TnA_GC
Cuts at: 3717 3717
Size: 7162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3881 5287 6152 6450 3881
Size: 1406 865 298 4593
Fragments arranged by size:
4593 1406 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2369 3947 4182 5825 882
Size: 617 870 1578 235 1643 2219
Fragments arranged by size:
2219 1643 1578 870 617 235
BsaAI yAC'GTr
Cuts at: 493 1204 4953 493
Size: 711 3749 2702
Fragments arranged by size:
3749 2702 711
BsaBI GATnn'nnATC
Cuts at: 2343 7156 2343
Size: 4813 2349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3678 4969 5351 5986
Size: 53 83 186 936 2145 1291 382 635
Cuts at: 5986 275
Size: 1451
Fragments arranged by size:
2145 1451 1291 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2117 5595 5993 6267 6414 6825 2117
Size: 3478 398 274 147 411 2454
Fragments arranged by size:
3478 2454 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3376 3406 4004 4034 1178
Size: 30 165 30 1973 30 598 30 4306
Fragments arranged by size:
4306 1973 598 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2273 2504 2621 2660 2738 2894 3116 3173
Size: 1087 231 117 39 78 156 222 57
Cuts at: 3173 3176 3923 4229 1186
Size: 3 747 306 4119
Fragments arranged by size:
4119 1087 747 306 231 222 156 117
78 57 39 3
BseYI C'CCAG_C
Cuts at: 3335 6365 6861 3335
Size: 3030 496 3636
Fragments arranged by size:
3636 3030 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2325 3876 4401 1163
Size: 573 589 1551 525 3924
Fragments arranged by size:
3924 1551 589 573 525
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4612 5373 6401 1540
Size: 275 186 2611 761 1028 2301
Fragments arranged by size:
2611 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2496 5223 5308 6379 2496
Size: 2727 85 1071 3279
Fragments arranged by size:
3279 2727 1071 85
BsmI GAATG_Cn'
Cuts at: 4673 4766 4673
Size: 93 7069
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2496 4255 4481 5223 5308 6379
Size: 399 436 270 1759 226 742 85 1071
Cuts at: 6379 1391
Size: 2174
Fragments arranged by size:
2174 1759 1071 742 436 399 270 226
85
BspEI T'CCGG_A
Cuts at: 2117 6825 2117
Size: 4708 2454
BspHI T'CATG_A
Cuts at: 5051 6781 5051
Size: 1730 5432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4517 844
Size: 3673 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5049 1394
Size: 429 3226 3507
Fragments arranged by size:
3507 3226 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3409 5657 5831 65
Size: 1183 2161 2248 174 1396
Fragments arranged by size:
2248 2161 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5806 5993 1700
Size: 110 3996 187 2869
Fragments arranged by size:
3996 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7162
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7162
BssSI C'ACGA_G
Cuts at: 5222 6234 5222
Size: 1012 6150
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3934 794
Size: 3140 4022
BstBI TT'CG_AA
Cuts at: 5966 5966
Size: 7162
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7162
BstYI r'GATC_y
Cuts at: 1972 2842 3373 3487 3900 3921 4126 4237 4412
Size: 870 531 114 413 21 205 111 175
Cuts at: 4412 4492 4859 5244 5261 6713 6799 6811 7157
Size: 80 367 385 17 1452 86 12 346
Cuts at: 7157 1972
Size: 1977
Fragments arranged by size:
1977 1452 870 531 413 385 367 346
205 175 114 111 86 80 21 17
12
Bsu36I CC'TnA_GG
Cuts at: 2661 2661
Size: 7162
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5671
Fragments arranged by size:
5671 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3660 487
Size: 683 56 2434 3989
Fragments arranged by size:
3989 2434 683 56
BtsI GCAGTG_nn'
Cuts at: 3990 4431 4678 5472 5492 3990
Size: 441 247 794 20 5660
Fragments arranged by size:
5660 794 441 247 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7127
DraI TTT'AAA
Cuts at: 4466 4466
Size: 7162
DraIII CAC_nnn'GTG
Cuts at: 4426 4426
Size: 7162
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4292 6169 809
Size: 507 2976 1877 1802
Fragments arranged by size:
2976 1877 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4609 5498 7127
Size: 54 1425 50 275 18 2779 889 1629
Cuts at: 7127 8
Size: 43
Fragments arranged by size:
2779 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4609 1537
Size: 275 2797 4090
Fragments arranged by size:
4090 2797 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2367 2495 2796 3170 3207 3227 3360 3447
Size: 1014 128 301 374 37 20 133 87
Cuts at: 3447 4180 5092 1353
Size: 733 912 3423
Fragments arranged by size:
3423 1014 912 733 374 301 133 128
87 37 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5749 6123 6269 387
Size: 1064 138 4160 374 146 1280
Fragments arranged by size:
4160 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2494 2494
Size: 7162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2480 3938 3980 4254 5225 5822 6609
Size: 393 848 1458 42 274 971 597 787
Cuts at: 6609 6877 1239
Size: 268 1524
Fragments arranged by size:
1524 1458 971 848 787 597 393 274
268 42
EcoNI CCTnn'n_nnAGG
Cuts at: 4196 4196
Size: 7162
EcoO109I rG'GnC_Cy
Cuts at: 2764 2921 2933 3808 4264 2764
Size: 157 12 875 456 5662
Fragments arranged by size:
5662 875 456 157 12
EcoRI G'AATT_C
Cuts at: 4471 4849 4471
Size: 378 6784
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3434 3434
Size: 7162
FspI TGC'GCA
Cuts at: 5668 5668
Size: 7162
HaeII r_GCGC'y
Cuts at: 1678 6309 1678
Size: 4631 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 2270 2276 2302
Size: 32 135 32 494 32 172 6 26
Cuts at: 2302 2308 2948 2980 3206 3238 3263 3295 3380
Size: 6 640 32 226 32 25 32 85
Cuts at: 3380 3412 3566 3598 4004 4036 5803 5835 5877
Size: 32 154 32 406 32 1767 32 42
Cuts at: 5877 5909 1373
Size: 32 2626
Fragments arranged by size:
2626 1767 640 494 406 226 172 154
135 85 42 32 32 32 32 32
32 32 32 32 32 32 26 25
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4364 4506 4752 669
Size: 988 281 2426 142 246 3079
Fragments arranged by size:
3079 2426 988 281 246 142
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7162
HpaI GTT'AAC
Cuts at: 4752 4752
Size: 7162
KpnI G_GTAC'C
Cuts at: 4487 4487
Size: 7162
MfeI C'AATT_G
Cuts at: 4761 4761
Size: 7162
MluI A'CGCG_T
Cuts at: 7038 7038
Size: 7162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6276 6460 1305
Size: 4971 184 2007
Fragments arranged by size:
4971 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5340
Fragments arranged by size:
5340 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2433 5120 5479 5638 518
Size: 648 1267 2687 359 159 2042
Fragments arranged by size:
2687 2042 1267 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2025 4578 5255 6403 6648 1606
Size: 419 2553 677 1148 245 2120
Fragments arranged by size:
2553 2120 1148 677 419 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5671
Fragments arranged by size:
5671 939 552
NdeI CA'TA_TG
Cuts at: 387 3066 3570 387
Size: 2679 504 3979
Fragments arranged by size:
3979 2679 504
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7162
NotI GC'GGCC_GC
Cuts at: 4609 4609
Size: 7162
NspI r_CATG'y
Cuts at: 1132 1229 2432 4520 6065 1132
Size: 97 1203 2088 1545 2229
Fragments arranged by size:
2229 2088 1545 1203 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7162
PciI A'CATG_T
Cuts at: 6061 6061
Size: 7162
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7162
PfoI T'CCnGG_A
Cuts at: 3903 4240 3903
Size: 337 6825
PmeI GTTT'AAAC
Cuts at: 4466 4466
Size: 7162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5231 5263 6744 6776 5231
Size: 32 1481 32 5617
Fragments arranged by size:
5617 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2764 3808 4264 2764
Size: 1044 456 5662
Fragments arranged by size:
5662 1044 456
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7162
PsiI TTA'TAA
Cuts at: 4732 4732
Size: 7162
PstI C_TGCA'G
Cuts at: 830 4514 830
Size: 3684 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7162
SacI G_AGCT'C
Cuts at: 2496 2496
Size: 7162
SalI G'TCGA_C
Cuts at: 1936 4362 4504 1936
Size: 2426 142 4594
Fragments arranged by size:
4594 2426 142
SanDI GG'GwC_CC
Cuts at: 4264 4264
Size: 7162
SapI GCTCTTCn'nnn_
Cuts at: 2796 2796
Size: 7162
SbfI CC_TGCA'GG
Cuts at: 4514 4514
Size: 7162
ScaI AGT'ACT
Cuts at: 5410 7122 5410
Size: 1712 5450
SexAI A'CCwGG_T
Cuts at: 2389 2389
Size: 7162
SfcI C'TryA_G
Cuts at: 826 1045 3783 4510 5645 6326 6517 826
Size: 219 2738 727 1135 681 191 1471
Fragments arranged by size:
2738 1471 1135 727 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7162
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7162
SmaI CCC'GGG
Cuts at: 1872 4489 1872
Size: 2617 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3797 3896 5266 6167 6429 820
Size: 197 926 1854 99 1370 901 262 1553
Fragments arranged by size:
1854 1553 1370 926 901 262 197 99
SnaBI TAC'GTA
Cuts at: 493 4953 493
Size: 4460 2702
SpeI A'CTAG_T
Cuts at: 152 2162 152
Size: 2010 5152
SphI G_CATG'C
Cuts at: 1132 2432 4520 1132
Size: 1300 2088 3774
Fragments arranged by size:
3774 2088 1300
SspI AAT'ATT
Cuts at: 5 52 2018 5086 5
Size: 47 1966 3068 2081
Fragments arranged by size:
3068 2081 1966 47
StuI AGG'CCT
Cuts at: 1517 4066 1517
Size: 2549 4613
StyI C'CwwG_G
Cuts at: 513 1065 2004 2576 2693 2849 4008 4601 513
Size: 552 939 572 117 156 1159 593 3074
Fragments arranged by size:
3074 1159 939 593 572 552 156 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4195 5189 5206 5359 5544 5989 325
Size: 1255 2615 994 17 153 185 445 1498
Fragments arranged by size:
2615 1498 1255 994 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2059 5408 7120
Size: 274 80 33 51 1443 82 3349 1712
Cuts at: 7120 96
Size: 138
Fragments arranged by size:
3349 1712 1443 274 138 82 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2184 2216 3376 3408 3885 3917 3969 4001 7122
Size: 32 1160 32 477 32 52 32 3121
Cuts at: 7122 7154 2184
Size: 32 2192
Fragments arranged by size:
3121 2192 1160 477 52 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3677 1316
Size: 2361 4801
XbaI T'CTAG_A
Cuts at: 4129 4498 4129
Size: 369 6793
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2039 4189 4309 2039
Size: 2150 120 4892
Fragments arranged by size:
4892 2150 120
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7162
XmaI C'CCGG_G
Cuts at: 1870 4487 1870
Size: 2617 4545
XmnI GAAnn'nnTTC
Cuts at: 2805 5291 2805
Size: 2486 4676
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3679 4970 276
Size: 53 83 186 936 2145 1291 2468
Fragments arranged by size:
2468 2145 1291 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacII SfiI SfoI
SrfI SwaI