Restriction map for
pFN21AE3304
(Circular) (Six-base) MAPSORT of: pFN21AE3304.seq Check: 7585 from: 1 to: 7090
pFN21AE3304 7090 bp
With 182 enzymes: SgfI *
March 28, 2012 14:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4900 278
Size: 53 83 186 936 3364 2468
Fragments arranged by size:
3364 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3463 4146 4433 1937
Size: 1526 683 287 4594
Fragments arranged by size:
4594 1526 683 287
Acc65I G'GTAC_C
Cuts at: 3053 4411 3053
Size: 1358 5732
AclI AA'CG_TT
Cuts at: 1563 3277 4044 4389 5217 5590 1563
Size: 1714 767 345 828 373 3063
Fragments arranged by size:
3063 1714 828 767 373 345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2068 2091 2225 3955 4236 5153 6537 6805
Size: 436 23 134 1730 281 917 1384 268
Cuts at: 6805 1632
Size: 1917
Fragments arranged by size:
1917 1730 1384 917 436 281 268 134
23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6893
AflIII A'CryG_T
Cuts at: 1203 2119 3361 3759 5989 6966 1203
Size: 916 1242 398 2230 977 1327
Fragments arranged by size:
2230 1327 1242 977 916 398
AgeI A'CCGG_T
Cuts at: 5921 5921
Size: 7090
AhdI GACnn_n'nnGTC
Cuts at: 5819 5819
Size: 7090
AleI CACnn'nnGTG
Cuts at: 1166 2196 1166
Size: 1030 6060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7090
AlwNI CAG_nnn'CTG
Cuts at: 2550 4155 4833 6405 2550
Size: 1605 678 1572 3235
Fragments arranged by size:
3235 1605 1572 678
ApaLI G'TGCA_C
Cuts at: 5147 6303 5147
Size: 1156 5934
ApoI r'AATT_y
Cuts at: 1493 3933 3950 4399 4625 4777 6886 1493
Size: 2440 17 449 226 152 2109 1697
Fragments arranged by size:
2440 2109 1697 449 226 152 17
AseI AT'TA_AT
Cuts at: 160 1032 5644 160
Size: 872 4612 1606
Fragments arranged by size:
4612 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7090
AvaI C'yCGr_G
Cuts at: 1870 1943 2253 2431 4248 4415 1870
Size: 73 310 178 1817 167 4545
Fragments arranged by size:
4545 1817 310 178 167 73
BamHI G'GATC_C
Cuts at: 4420 4787 4420
Size: 367 6723
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3053 4411 5866 618
Size: 325 217 627 1266 1358 1455 1842
Fragments arranged by size:
1842 1455 1358 1266 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2697 4409 1391
Size: 1306 1712 4072
Fragments arranged by size:
4072 1712 1306
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2074 928
Size: 971 175 5944
Fragments arranged by size:
5944 971 175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2167 2201 3364 3398 5281 5315 1903
Size: 34 230 34 1163 34 1883 34 3678
Fragments arranged by size:
3678 1883 1163 230 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2108 4982 6192 2108
Size: 2874 1210 3006
Fragments arranged by size:
3006 2874 1210
BclI T'GATC_A
Cuts at: 1548 3666 1548
Size: 2118 4972
BfrBI ATG'CAT
Cuts at: 4026 4026
Size: 7090
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2762 2954 5701
Size: 107 122 71 631 605 1090 192 2747
Cuts at: 5701 136
Size: 1525
Fragments arranged by size:
2747 1525 1090 631 605 192 122 107
71
BglII A'GATC_T
Cuts at: 2087 3634 3661 7085 2087
Size: 1547 27 3424 2092
Fragments arranged by size:
3424 2092 1547 27
BlpI GC'TnA_GC
Cuts at: 2691 4507 2691
Size: 1816 5274
Bme1580I G_kGCm'C
Cuts at: 1790 3018 3741 5151 6307 1790
Size: 1228 723 1410 1156 2573
Fragments arranged by size:
2573 1410 1228 1156 723
BmgBI CAC'GTC
Cuts at: 1318 3019 3362 1318
Size: 1701 343 5046
Fragments arranged by size:
5046 1701 343
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2533 4217 4821
Size: 416 53 83 399 24 1116 1684 604
Cuts at: 4821 5779 442
Size: 958 1753
Fragments arranged by size:
1753 1684 1116 958 604 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7090
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3592 3592
Size: 7090
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3141 5750 1239
Size: 1902 2609 2579
Fragments arranged by size:
2609 2579 1902
Bpu10I CC'TnA_GC
Cuts at: 3150 3717 3878 3150
Size: 567 161 6362
Fragments arranged by size:
6362 567 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4286 5215 6080 6378 4286
Size: 929 865 298 4998
Fragments arranged by size:
4998 929 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4321 5753 882
Size: 617 2822 1432 2219
Fragments arranged by size:
2822 2219 1432 617
BsaAI yAC'GTr
Cuts at: 493 1204 3677 4144 4312 4881 493
Size: 711 2473 467 168 569 2702
Fragments arranged by size:
2702 2473 711 569 467 168
BsaBI GATnn'nnATC
Cuts at: 7084 7084
Size: 7090
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4897 5279 5914 275
Size: 53 83 186 936 3364 382 635 1451
Fragments arranged by size:
3364 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3543 5523 5921 6195 6342 6753 3543
Size: 1980 398 274 147 411 3880
Fragments arranged by size:
3880 1980 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3842 3872 4203 4233 1178
Size: 30 165 30 2439 30 331 30 4035
Fragments arranged by size:
4035 2439 331 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3254 3255 3331 1186
Size: 2068 1 76 4945
Fragments arranged by size:
4945 2068 76 1
BseYI C'CCAG_C
Cuts at: 2686 3687 6293 6789 2686
Size: 1001 2606 496 2987
Fragments arranged by size:
2987 2606 1001 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2508 3516 1163
Size: 573 772 1008 4737
Fragments arranged by size:
4737 1008 772 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4540 5301 6329 1540
Size: 275 186 2539 761 1028 2301
Fragments arranged by size:
2539 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5151 5236 6307 5151
Size: 85 1071 5934
Fragments arranged by size:
5934 1071 85
BsmI GAATG_Cn'
Cuts at: 4174 4601 4694 4174
Size: 427 93 6570
Fragments arranged by size:
6570 427 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2697 3018 3741 4409 5151 5236 6307
Size: 399 907 321 723 668 742 85 1071
Cuts at: 6307 1391
Size: 2174
Fragments arranged by size:
2174 1071 907 742 723 668 399 321
85
BspEI T'CCGG_A
Cuts at: 6753 6753
Size: 7090
BspHI T'CATG_A
Cuts at: 2626 4354 4979 6709 2626
Size: 1728 625 1730 3007
Fragments arranged by size:
3007 1730 1728 625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2296 2715 4445 844
Size: 1452 419 1730 3489
Fragments arranged by size:
3489 1730 1452 419
BsrBI CCG'CTC
Cuts at: 1394 1823 4977 1394
Size: 429 3154 3507
Fragments arranged by size:
3507 3154 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3505 5585 5759 65
Size: 1183 2257 2080 174 1396
Fragments arranged by size:
2257 2080 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5734 5921 1700
Size: 110 3924 187 2869
Fragments arranged by size:
3924 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2072 96
Size: 1976 5114
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7090
BssSI C'ACGA_G
Cuts at: 2478 5150 6162 2478
Size: 2672 1012 3406
Fragments arranged by size:
3406 2672 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7090
BstBI TT'CG_AA
Cuts at: 5894 5894
Size: 7090
BstEII G'GTnAC_C
Cuts at: 1184 2248 2347 2569 3793 4193 1184
Size: 1064 99 222 1224 400 4081
Fragments arranged by size:
4081 1224 1064 400 222 99
BstXI CCAn_nnnn'nTGG
Cuts at: 3394 4076 3394
Size: 682 6408
BstYI r'GATC_y
Cuts at: 1972 2087 2394 3210 3634 3661 3750 3871 4420
Size: 115 307 816 424 27 89 121 549
Cuts at: 4420 4787 5172 5189 6641 6727 6739 7085 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 816 549 424 385 367 346
307 121 115 89 86 27 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2244 3583 513
Size: 552 1179 1339 4020
Fragments arranged by size:
4020 1339 1179 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6351
Fragments arranged by size:
6351 683 56
BtsI GCAGTG_nn'
Cuts at: 2203 2245 4606 5400 5420 2203
Size: 42 2361 794 20 3873
Fragments arranged by size:
3873 2361 794 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7090
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7055
DraI TTT'AAA
Cuts at: 4344 4394 4344
Size: 50 7040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4313 6097 809
Size: 507 2997 1784 1802
Fragments arranged by size:
2997 1802 1784 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3135 3312 4537
Size: 54 1425 50 275 18 1305 177 1225
Cuts at: 4537 5426 7055 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1305 1225 889 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4537 1537
Size: 275 2725 4090
Fragments arranged by size:
4090 2725 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3688 5020 1353
Size: 2335 1332 3423
Fragments arranged by size:
3423 2335 1332
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5677 6051 6197 387
Size: 1064 138 4088 374 146 1280
Fragments arranged by size:
4088 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2091 2225 3141 3955 4236 5153
Size: 393 436 23 134 916 814 281 917
Cuts at: 5153 5750 6537 6805 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 917 916 814 787 597 436 393
281 268 134 23
EcoNI CCTnn'n_nnAGG
Cuts at: 3563 3563
Size: 7090
EcoO109I rG'GnC_Cy
Cuts at: 2422 3083 3944 4012 2422
Size: 661 861 68 5500
Fragments arranged by size:
5500 861 661 68
EcoRI G'AATT_C
Cuts at: 4399 4777 4399
Size: 378 6712
FspI TGC'GCA
Cuts at: 5596 5596
Size: 7090
HaeII r_GCGC'y
Cuts at: 1678 2287 2605 6237 1678
Size: 609 318 3632 2531
Fragments arranged by size:
3632 2531 609 318
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2294 2326 2867 2899 5731
Size: 32 135 32 722 32 541 32 2832
Cuts at: 5731 5763 5805 5837 1373
Size: 32 42 32 2626
Fragments arranged by size:
2832 2626 722 541 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2662 3192 4434 4680 669
Size: 988 281 724 530 1242 246 3079
Fragments arranged by size:
3079 1242 988 724 530 281 246
HindIII A'AGCT_T
Cuts at: 748 2849 748
Size: 2101 4989
HpaI GTT'AAC
Cuts at: 4680 4680
Size: 7090
KpnI G_GTAC'C
Cuts at: 3057 4415 3057
Size: 1358 5732
MfeI C'AATT_G
Cuts at: 3420 4689 3420
Size: 1269 5821
MluI A'CGCG_T
Cuts at: 6966 6966
Size: 7090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6204 6388 1305
Size: 4899 184 2007
Fragments arranged by size:
4899 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3137 3314 10
Size: 54 1425 343 1305 177 3786
Fragments arranged by size:
3786 1425 1305 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2196 3762 3764 3908 4074 4092 5048
Size: 648 1030 1566 2 144 166 18 956
Cuts at: 5048 5407 5566 518
Size: 359 159 2042
Fragments arranged by size:
2042 1566 1030 956 648 359 166 159
144 18 2
MspA1I CmG'CkG
Cuts at: 1606 2025 2446 2492 2690 3691 3833 4124 4506
Size: 419 421 46 198 1001 142 291 382
Cuts at: 4506 5183 6331 6576 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1001 677 421 419 382 291
245 198 142 46
NcoI C'CATG_G
Cuts at: 513 1065 3583 513
Size: 552 2518 4020
Fragments arranged by size:
4020 2518 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7090
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7090
NotI GC'GGCC_GC
Cuts at: 4537 4537
Size: 7090
NsiI A_TGCA'T
Cuts at: 4028 4028
Size: 7090
NspI r_CATG'y
Cuts at: 1132 1229 2123 2213 3763 4448 5993 1132
Size: 97 894 90 1550 685 1545 2229
Fragments arranged by size:
2229 1550 1545 894 685 97 90
PasI CC'CwG_GG
Cuts at: 1348 2061 2278 1348
Size: 713 217 6160
Fragments arranged by size:
6160 713 217
PciI A'CATG_T
Cuts at: 2119 3759 5989 2119
Size: 1640 2230 3220
Fragments arranged by size:
3220 2230 1640
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2566 3178 1108
Size: 1458 612 5020
Fragments arranged by size:
5020 1458 612
PfoI T'CCnGG_A
Cuts at: 2869 2869
Size: 7090
PmeI GTTT'AAAC
Cuts at: 4394 4394
Size: 7090
PmlI CAC'GTG
Cuts at: 3677 4312 3677
Size: 635 6455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5159 5191 6672 6704 5159
Size: 32 1481 32 5545
Fragments arranged by size:
5545 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4012 4012
Size: 7090
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7090
PsiI TTA'TAA
Cuts at: 4660 4660
Size: 7090
PstI C_TGCA'G
Cuts at: 830 2322 2712 3082 4140 4442 830
Size: 1492 390 370 1058 302 3478
Fragments arranged by size:
3478 1492 1058 390 370 302
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7090
PvuII CAG'CTG
Cuts at: 2446 2492 2690 3691 3833 2446
Size: 46 198 1001 142 5703
Fragments arranged by size:
5703 1001 198 142 46
SalI G'TCGA_C
Cuts at: 1936 4432 1936
Size: 2496 4594
SapI GCTCTTCn'nnn_
Cuts at: 3688 3688
Size: 7090
SbfI CC_TGCA'GG
Cuts at: 2712 3082 4442 2712
Size: 370 1360 5360
Fragments arranged by size:
5360 1360 370
ScaI AGT'ACT
Cuts at: 5338 7050 5338
Size: 1712 5378
SfcI C'TryA_G
Cuts at: 826 1045 2318 2708 3078 4136 4438 5573 6254
Size: 219 1273 390 370 1058 302 1135 681
Cuts at: 6254 6445 826
Size: 191 1471
Fragments arranged by size:
1471 1273 1135 1058 681 390 370 302
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7090
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7090
SmaI CCC'GGG
Cuts at: 1872 4250 4417 1872
Size: 2378 167 4545
Fragments arranged by size:
4545 2378 167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4265 5194 6095 6357 820
Size: 197 926 2322 929 901 262 1553
Fragments arranged by size:
2322 1553 929 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4881 493
Size: 4388 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7090
SphI G_CATG'C
Cuts at: 1132 2213 4448 1132
Size: 1081 2235 3774
Fragments arranged by size:
3774 2235 1081
SspI AAT'ATT
Cuts at: 5 52 5014 5
Size: 47 4962 2081
Fragments arranged by size:
4962 2081 47
StuI AGG'CCT
Cuts at: 1517 2814 1517
Size: 1297 5793
StyI C'CwwG_G
Cuts at: 513 1065 2934 3141 3583 4529 513
Size: 552 1869 207 442 946 3074
Fragments arranged by size:
3074 1869 946 552 442 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2909 3015 3308 5117 5134 5287 5472
Size: 1255 1329 106 293 1809 17 153 185
Cuts at: 5472 5917 325
Size: 445 1498
Fragments arranged by size:
1809 1498 1329 1255 445 293 185 153
106 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2072 2541 4229
Size: 274 80 33 51 1443 95 469 1688
Cuts at: 4229 5336 7048 96
Size: 1107 1712 138
Fragments arranged by size:
1712 1688 1443 1107 469 274 138 95
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4203 4235 7050 7082 4203
Size: 32 2815 32 4211
Fragments arranged by size:
4211 2815 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7090
XbaI T'CTAG_A
Cuts at: 2906 4426 2906
Size: 1520 5570
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2221 2908 2221
Size: 687 6403
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7090
XmaI C'CCGG_G
Cuts at: 1870 4248 4415 1870
Size: 2378 167 4545
Fragments arranged by size:
4545 2378 167
XmnI GAAnn'nnTTC
Cuts at: 5219 5219
Size: 7090
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4898 276
Size: 53 83 186 936 3364 2468
Fragments arranged by size:
3364 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI