Restriction map for
pFN21AE3303
(Circular) (Six-base) MAPSORT of: pFN21AE3303.seq Check: 8687 from: 1 to: 6961
pFN21AE3303 6961 bp
With 182 enzymes: SgfI *
March 28, 2012 12:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4771 278
Size: 53 83 186 936 3235 2468
Fragments arranged by size:
3235 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2481 4304 1937
Size: 544 1823 4594
Fragments arranged by size:
4594 1823 544
Acc65I G'GTAC_C
Cuts at: 3179 4282 3179
Size: 1103 5858
AclI AA'CG_TT
Cuts at: 1563 3029 5088 5461 1563
Size: 1466 2059 373 3063
Fragments arranged by size:
3063 2059 1466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2224 2430 2507 4076 5024 6408 6676 1632
Size: 592 206 77 1569 948 1384 268 1917
Fragments arranged by size:
1917 1569 1384 948 592 268 206 77
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6764
AflIII A'CryG_T
Cuts at: 1203 5860 6837 1203
Size: 4657 977 1327
Fragments arranged by size:
4657 1327 977
AgeI A'CCGG_T
Cuts at: 5792 5792
Size: 6961
AhdI GACnn_n'nnGTC
Cuts at: 5690 5690
Size: 6961
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6961
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3787 1184
Size: 2603 4358
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 4234 4266 2430
Size: 32 1772 32 5125
Fragments arranged by size:
5125 1772 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2215 2644 3431 4704 6276 2215
Size: 429 787 1273 1572 2900
Fragments arranged by size:
2900 1572 1273 787 429
ApaLI G'TGCA_C
Cuts at: 5018 6174 5018
Size: 1156 5805
ApoI r'AATT_y
Cuts at: 1493 2042 2105 3237 3248 3701 3740 3968 4270
Size: 549 63 1132 11 453 39 228 302
Cuts at: 4270 4496 4648 6757 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1132 549 453 302 228 226
152 63 39 11
AseI AT'TA_AT
Cuts at: 160 1032 5515 160
Size: 872 4483 1606
Fragments arranged by size:
4483 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6961
AvaI C'yCGr_G
Cuts at: 1870 1943 3093 3175 4286 1870
Size: 73 1150 82 1111 4545
Fragments arranged by size:
4545 1150 1111 82 73
BamHI G'GATC_C
Cuts at: 2653 4291 4658 2653
Size: 1638 367 4956
Fragments arranged by size:
4956 1638 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3179 3746 3884 4282 5737
Size: 325 217 627 1392 567 138 398 1455
Cuts at: 5737 618
Size: 1842
Fragments arranged by size:
1842 1455 1392 627 567 398 325 217
138
BanII G_rGCy'C
Cuts at: 1391 2432 3539 4280 1391
Size: 1041 1107 741 4072
Fragments arranged by size:
4072 1107 1041 741
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2158 2418 2448 928
Size: 971 259 260 30 5441
Fragments arranged by size:
5441 971 260 259 30
BbvCI CC'TCA_GC
Cuts at: 2666 2666
Size: 6961
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2345 2379 5152 5186 1903
Size: 34 408 34 2773 34 3678
Fragments arranged by size:
3678 2773 408 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4853 6063 4853
Size: 1210 5751
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6961
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5572 136
Size: 107 122 71 631 605 3900 1525
Fragments arranged by size:
3900 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4041 6956 4041
Size: 2915 4046
BlpI GC'TnA_GC
Cuts at: 4378 4378
Size: 6961
Bme1580I G_kGCm'C
Cuts at: 1790 2368 5022 6178 1790
Size: 578 2654 1156 2573
Fragments arranged by size:
2654 2573 1156 578
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6961
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2468 2632 4075
Size: 416 53 83 399 24 1051 164 1443
Cuts at: 4075 4692 5650 442
Size: 617 958 1753
Fragments arranged by size:
1753 1443 1051 958 617 416 399 164
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6961
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2019 2039 2213 2537 2564 2666 3291 3318
Size: 780 20 174 324 27 102 625 27
Cuts at: 3318 5621 1239
Size: 2303 2579
Fragments arranged by size:
2579 2303 780 625 324 174 102 27
27 20
Bpu10I CC'TnA_GC
Cuts at: 2666 3827 2666
Size: 1161 5800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5086 5951 6249 5086
Size: 865 298 5798
Fragments arranged by size:
5798 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2029 2255 2588 3048 5624 882
Size: 617 530 226 333 460 2576 2219
Fragments arranged by size:
2576 2219 617 530 460 333 226
BsaAI yAC'GTr
Cuts at: 493 1204 4752 493
Size: 711 3548 2702
Fragments arranged by size:
3548 2702 711
BsaBI GATnn'nnATC
Cuts at: 6955 6955
Size: 6961
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2886 4768 5150 5785
Size: 53 83 186 936 1353 1882 382 635
Cuts at: 5785 275
Size: 1451
Fragments arranged by size:
1882 1451 1353 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5394 5792 6066 6213 6624 5394
Size: 398 274 147 411 5731
Fragments arranged by size:
5731 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2537 2567 2783 2813 3620
Size: 30 165 30 1134 30 216 30 807
Cuts at: 3620 3650 1178
Size: 30 4489
Fragments arranged by size:
4489 1134 807 216 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3498 1186
Size: 2312 4649
BseYI C'CCAG_C
Cuts at: 2208 3477 3869 6164 6660 2208
Size: 1269 392 2295 496 2509
Fragments arranged by size:
2509 2295 1269 496 392
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2893 4083 1163
Size: 573 1157 1190 4041
Fragments arranged by size:
4041 1190 1157 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2710 4411 5172 6200 1540
Size: 275 186 709 1701 761 1028 2301
Fragments arranged by size:
2301 1701 1028 761 709 275 186
BsiHKAI G_wGCw'C
Cuts at: 2222 5022 5107 6178 2222
Size: 2800 85 1071 3005
Fragments arranged by size:
3005 2800 1071 85
BsmI GAATG_Cn'
Cuts at: 2335 4472 4565 2335
Size: 2137 93 4731
Fragments arranged by size:
4731 2137 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2513 2513
Size: 6961
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2222 2368 2432 3539 4280 5022 5107
Size: 399 432 146 64 1107 741 742 85
Cuts at: 5107 6178 1391
Size: 1071 2174
Fragments arranged by size:
2174 1107 1071 742 741 432 399 146
85 64
BspEI T'CCGG_A
Cuts at: 6624 6624
Size: 6961
BspHI T'CATG_A
Cuts at: 2178 3621 4850 6580 2178
Size: 1443 1229 1730 2559
Fragments arranged by size:
2559 1730 1443 1229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3779 4316 844
Size: 2935 537 3489
Fragments arranged by size:
3489 2935 537
BsrBI CCG'CTC
Cuts at: 1394 1823 4848 1394
Size: 429 3025 3507
Fragments arranged by size:
3507 3025 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2765 3657 3849 5456 5630 65
Size: 1183 1517 892 192 1607 174 1396
Fragments arranged by size:
1607 1517 1396 1183 892 192 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4049 5605 5792 1700
Size: 110 2239 1556 187 2869
Fragments arranged by size:
2869 2239 1556 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6961
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6961
BssSI C'ACGA_G
Cuts at: 2258 5021 6033 2258
Size: 2763 1012 3186
Fragments arranged by size:
3186 2763 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2339 2865 794
Size: 1545 526 4890
Fragments arranged by size:
4890 1545 526
BstBI TT'CG_AA
Cuts at: 5765 5765
Size: 6961
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6961
BstXI CCAn_nnnn'nTGG
Cuts at: 3646 4033 4201 3646
Size: 387 168 6406
Fragments arranged by size:
6406 387 168
BstYI r'GATC_y
Cuts at: 1972 2653 4041 4291 4658 5043 5060 6512 6598
Size: 681 1388 250 367 385 17 1452 86
Cuts at: 6598 6610 6956 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1388 681 385 367 346 250
86 17 12
BstZ17I GTA'TAC
Cuts at: 2482 2482
Size: 6961
Bsu36I CC'TnA_GG
Cuts at: 2532 2532
Size: 6961
BtgI C'CryG_G
Cuts at: 513 1065 2004 2743 4026 513
Size: 552 939 739 1283 3448
Fragments arranged by size:
3448 1283 939 739 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6222
Fragments arranged by size:
6222 683 56
BtsI GCAGTG_nn'
Cuts at: 4477 5271 5291 4477
Size: 794 20 6147
Fragments arranged by size:
6147 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6961
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2977 3012 4110 4145 529
Size: 35 2413 35 1098 35 3345
Fragments arranged by size:
3345 2413 1098 35 35 35
DraI TTT'AAA
Cuts at: 4265 4265
Size: 6961
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5968 809
Size: 507 4652 1802
Fragments arranged by size:
4652 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4408 5297 6926
Size: 54 1425 50 275 18 2578 889 1629
Cuts at: 6926 8
Size: 43
Fragments arranged by size:
2578 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4408 1537
Size: 275 2596 4090
Fragments arranged by size:
4090 2596 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2234 4052 4211 4238 4891 1353
Size: 881 1818 159 27 653 3423
Fragments arranged by size:
3423 1818 881 653 159 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3121 5548 5922 6068 387
Size: 1064 138 1532 2427 374 146 1280
Fragments arranged by size:
2427 1532 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2019 2039 2213 2224 2430 2507 2537
Size: 393 387 20 174 11 206 77 30
Cuts at: 2537 2564 2666 3291 3318 4076 5024 5621 6408
Size: 27 102 625 27 758 948 597 787
Cuts at: 6408 6676 1239
Size: 268 1524
Fragments arranged by size:
1524 948 787 758 625 597 393 387
268 206 174 102 77 30 27 27
20 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3306 3306
Size: 6961
EcoO109I rG'GnC_Cy
Cuts at: 3299 3607 4205 3299
Size: 308 598 6055
Fragments arranged by size:
6055 598 308
EcoRI G'AATT_C
Cuts at: 3237 4270 4648 3237
Size: 1033 378 5550
Fragments arranged by size:
5550 1033 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3424 4240 3424
Size: 816 6145
FspI TGC'GCA
Cuts at: 5467 5467
Size: 6961
HaeII r_GCGC'y
Cuts at: 1678 4193 6108 1678
Size: 2515 1915 2531
Fragments arranged by size:
2531 2515 1915
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 2783 2815 3045
Size: 32 135 32 791 32 388 32 230
Cuts at: 3045 3077 4192 4224 5602 5634 5676 5708 1373
Size: 32 1115 32 1378 32 42 32 2626
Fragments arranged by size:
2626 1378 1115 791 388 230 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2617 4305 4551 669
Size: 988 281 679 1688 246 3079
Fragments arranged by size:
3079 1688 988 679 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6961
HpaI GTT'AAC
Cuts at: 4551 4551
Size: 6961
KpnI G_GTAC'C
Cuts at: 3183 4286 3183
Size: 1103 5858
MfeI C'AATT_G
Cuts at: 4560 4560
Size: 6961
MluI A'CGCG_T
Cuts at: 6837 6837
Size: 6961
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3780 6075 6259 1305
Size: 2475 2295 184 2007
Fragments arranged by size:
2475 2295 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5139
Fragments arranged by size:
5139 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2879 3644 4031 4199 4919 5278 5437
Size: 648 1713 765 387 168 720 359 159
Cuts at: 5437 518
Size: 2042
Fragments arranged by size:
2042 1713 765 720 648 387 359 168
159
MspA1I CmG'CkG
Cuts at: 1606 2408 3477 3558 4377 5054 6202 6447 1606
Size: 802 1069 81 819 677 1148 245 2120
Fragments arranged by size:
2120 1148 1069 819 802 677 245 81
NcoI C'CATG_G
Cuts at: 513 1065 2004 2743 4026 513
Size: 552 939 739 1283 3448
Fragments arranged by size:
3448 1283 939 739 552
NdeI CA'TA_TG
Cuts at: 387 3080 387
Size: 2693 4268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6961
NotI GC'GGCC_GC
Cuts at: 4408 4408
Size: 6961
NspI r_CATG'y
Cuts at: 1132 1229 3877 4319 5864 1132
Size: 97 2648 442 1545 2229
Fragments arranged by size:
2648 2229 1545 442 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6961
PciI A'CATG_T
Cuts at: 5860 5860
Size: 6961
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2644 3894 1108
Size: 1536 1250 4175
Fragments arranged by size:
4175 1536 1250
PfoI T'CCnGG_A
Cuts at: 2586 3840 3927 2586
Size: 1254 87 5620
Fragments arranged by size:
5620 1254 87
PmeI GTTT'AAAC
Cuts at: 4265 4265
Size: 6961
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5030 5062 6543 6575 5030
Size: 32 1481 32 5416
Fragments arranged by size:
5416 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3607 3607
Size: 6961
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6961
PsiI TTA'TAA
Cuts at: 4531 4531
Size: 6961
PstI C_TGCA'G
Cuts at: 830 4313 830
Size: 3483 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6961
PvuII CAG'CTG
Cuts at: 2408 2408
Size: 6961
SalI G'TCGA_C
Cuts at: 1936 4303 1936
Size: 2367 4594
SapI GCTCTTCn'nnn_
Cuts at: 4211 4238 4211
Size: 27 6934
SbfI CC_TGCA'GG
Cuts at: 4313 4313
Size: 6961
ScaI AGT'ACT
Cuts at: 5209 6921 5209
Size: 1712 5249
SexAI A'CCwGG_T
Cuts at: 3603 3603
Size: 6961
SfcI C'TryA_G
Cuts at: 826 1045 2729 4309 5444 6125 6316 826
Size: 219 1684 1580 1135 681 191 1471
Fragments arranged by size:
1684 1580 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6961
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6961
SmaI CCC'GGG
Cuts at: 1872 3095 3177 4288 1872
Size: 1223 82 1111 4545
Fragments arranged by size:
4545 1223 1111 82
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5065 5966 6228 820
Size: 197 926 3122 901 262 1553
Fragments arranged by size:
3122 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4752 493
Size: 4259 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6961
SphI G_CATG'C
Cuts at: 1132 4319 1132
Size: 3187 3774
SspI AAT'ATT
Cuts at: 5 52 2063 2072 4885 5
Size: 47 2011 9 2813 2081
Fragments arranged by size:
2813 2081 2011 47 9
StuI AGG'CCT
Cuts at: 1517 2360 2530 2671 2901 1517
Size: 843 170 141 230 5577
Fragments arranged by size:
5577 843 230 170 141
StyI C'CwwG_G
Cuts at: 513 1065 2004 2743 2802 3135 3540 4026 4400
Size: 552 939 739 59 333 405 486 374
Cuts at: 4400 513
Size: 3074
Fragments arranged by size:
3074 939 739 552 486 405 374 333
59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2442 4988 5005 5158 5343 5788
Size: 1255 417 445 2546 17 153 185 445
Cuts at: 5788 325
Size: 1498
Fragments arranged by size:
2546 1498 1255 445 445 417 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5207 6919 96
Size: 274 80 33 51 1443 3230 1712 138
Fragments arranged by size:
3230 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2483 2515 3654 3686 6921 6953 2483
Size: 32 1139 32 3235 32 2491
Fragments arranged by size:
3235 2491 1139 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3605 1316
Size: 2289 4672
XbaI T'CTAG_A
Cuts at: 4297 4297
Size: 6961
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2767 3586 2767
Size: 819 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6961
XmaI C'CCGG_G
Cuts at: 1870 3093 3175 4286 1870
Size: 1223 82 1111 4545
Fragments arranged by size:
4545 1223 1111 82
XmnI GAAnn'nnTTC
Cuts at: 2837 3237 3320 5090 2837
Size: 400 83 1770 4708
Fragments arranged by size:
4708 1770 400 83
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4769 276
Size: 53 83 186 936 3235 2468
Fragments arranged by size:
3235 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI DraIII EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI