Restriction map for
pFN21AE3296
(Circular) (Six-base) MAPSORT of: pFN21AE3296.seq Check: 7132 from: 1 to: 7027
pFN21AE3296 7027 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2620 2726 2620
Size: 106 6921
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3625 4837 278
Size: 53 83 186 936 2089 1212 2468
Fragments arranged by size:
2468 2089 1212 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4370 1937
Size: 2433 4594
Acc65I G'GTAC_C
Cuts at: 2149 4348 2149
Size: 2199 4828
AclI AA'CG_TT
Cuts at: 1563 5154 5527 1563
Size: 3591 373 3063
Fragments arranged by size:
3591 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2283 2640 3113 5090 6474 6742 1632
Size: 651 357 473 1977 1384 268 1917
Fragments arranged by size:
1977 1917 1384 651 473 357 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6830
AflIII A'CryG_T
Cuts at: 1203 2762 5926 6903 1203
Size: 1559 3164 977 1327
Fragments arranged by size:
3164 1559 1327 977
AgeI A'CCGG_T
Cuts at: 5858 5858
Size: 7027
AhdI GACnn_n'nnGTC
Cuts at: 3626 5756 3626
Size: 2130 4897
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7027
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3367 3746 1184
Size: 2183 379 4465
Fragments arranged by size:
4465 2183 379
AlwNI CAG_nnn'CTG
Cuts at: 2072 2174 4770 6342 2072
Size: 102 2596 1572 2757
Fragments arranged by size:
2757 2596 1572 102
ApaLI G'TGCA_C
Cuts at: 3172 5084 6240 3172
Size: 1912 1156 3959
Fragments arranged by size:
3959 1912 1156
ApoI r'AATT_y
Cuts at: 1493 2408 3232 4174 4336 4562 4714 6823 1493
Size: 915 824 942 162 226 152 2109 1697
Fragments arranged by size:
2109 1697 942 915 824 226 162 152
AseI AT'TA_AT
Cuts at: 160 1032 5581 160
Size: 872 4549 1606
Fragments arranged by size:
4549 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7027
AvaI C'yCGr_G
Cuts at: 1870 1943 4352 1870
Size: 73 2409 4545
Fragments arranged by size:
4545 2409 73
BamHI G'GATC_C
Cuts at: 4357 4724 4357
Size: 367 6660
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2149 4348 5803 618
Size: 325 217 627 362 2199 1455 1842
Fragments arranged by size:
2199 1842 1455 627 362 325 217
BanII G_rGCy'C
Cuts at: 1391 3254 4346 1391
Size: 1863 1092 4072
Fragments arranged by size:
4072 1863 1092
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2274 928
Size: 971 375 5681
Fragments arranged by size:
5681 971 375
BbvCI CC'TCA_GC
Cuts at: 2178 3799 2178
Size: 1621 5406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5218 5252 1903
Size: 34 3281 34 3678
Fragments arranged by size:
3678 3281 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4919 6129 4919
Size: 1210 5817
BclI T'GATC_A
Cuts at: 1548 2184 1548
Size: 636 6391
BfrBI ATG'CAT
Cuts at: 3490 3490
Size: 7027
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5638 136
Size: 107 122 71 631 605 3966 1525
Fragments arranged by size:
3966 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7022 7022
Size: 7027
BlpI GC'TnA_GC
Cuts at: 4444 4444
Size: 7027
Bme1580I G_kGCm'C
Cuts at: 1790 3176 5088 6244 1790
Size: 1386 1912 1156 2573
Fragments arranged by size:
2573 1912 1386 1156
BmgBI CAC'GTC
Cuts at: 1318 3709 1318
Size: 2391 4636
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2983 4167 4758
Size: 416 53 83 399 24 1566 1184 591
Cuts at: 4758 5716 442
Size: 958 1753
Fragments arranged by size:
1753 1566 1184 958 591 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7027
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3958 3958
Size: 7027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2147 2198 2727 3268 3605 3652 4199
Size: 857 51 51 529 541 337 47 547
Cuts at: 4199 5687 1239
Size: 1488 2579
Fragments arranged by size:
2579 1488 857 547 541 529 337 51
51 47
Bpu10I CC'TnA_GC
Cuts at: 2178 3107 3269 3799 2178
Size: 929 162 530 5406
Fragments arranged by size:
5406 929 530 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5152 6017 6315 5152
Size: 865 298 5864
Fragments arranged by size:
5864 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2410 2505 3775 5690 882
Size: 617 911 95 1270 1915 2219
Fragments arranged by size:
2219 1915 1270 911 617 95
BsaAI yAC'GTr
Cuts at: 493 1204 2958 4818 493
Size: 711 1754 1860 2702
Fragments arranged by size:
2702 1860 1754 711
BsaBI GATnn'nnATC
Cuts at: 2328 3978 7021 2328
Size: 1650 3043 2334
Fragments arranged by size:
3043 2334 1650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3622 4834 5216 5851
Size: 53 83 186 936 2089 1212 382 635
Cuts at: 5851 275
Size: 1451
Fragments arranged by size:
2089 1451 1212 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5460 5858 6132 6279 6690 5460
Size: 398 274 147 411 5797
Fragments arranged by size:
5797 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2091 2121 2196 2226 2670
Size: 30 165 30 688 30 75 30 444
Cuts at: 2670 2700 1178
Size: 30 5505
Fragments arranged by size:
5505 688 444 165 75 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 3399 1186
Size: 913 1300 4814
Fragments arranged by size:
4814 1300 913
BseYI C'CCAG_C
Cuts at: 2341 2565 2894 6230 6726 2341
Size: 224 329 3336 496 2642
Fragments arranged by size:
3336 2642 496 329 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2060 3752 1163
Size: 573 324 1692 4438
Fragments arranged by size:
4438 1692 573 324
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4477 5238 6266 1540
Size: 275 186 2476 761 1028 2301
Fragments arranged by size:
2476 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2533 3176 5088 5173 6244 2533
Size: 643 1912 85 1071 3316
Fragments arranged by size:
3316 1912 1071 643 85
BsmI GAATG_Cn'
Cuts at: 2356 4538 4631 2356
Size: 2182 93 4752
Fragments arranged by size:
4752 2182 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2533 3176 3254 4346 5088 5173 6244
Size: 399 743 643 78 1092 742 85 1071
Cuts at: 6244 1391
Size: 2174
Fragments arranged by size:
2174 1092 1071 743 742 643 399 85
78
BspEI T'CCGG_A
Cuts at: 6690 6690
Size: 7027
BspHI T'CATG_A
Cuts at: 2011 4916 6646 2011
Size: 2905 1730 2392
Fragments arranged by size:
2905 2392 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2620 2726 3145 4382 844
Size: 1776 106 419 1237 3489
Fragments arranged by size:
3489 1776 1237 419 106
BsrBI CCG'CTC
Cuts at: 1394 1823 4914 1394
Size: 429 3091 3507
Fragments arranged by size:
3507 3091 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2025 5522 5696 65
Size: 1183 777 3497 174 1396
Fragments arranged by size:
3497 1396 1183 777 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2904 5671 5858 1700
Size: 110 1094 2767 187 2869
Fragments arranged by size:
2869 2767 1094 187 110
BsrGI T'GTAC_A
Cuts at: 96 2864 4063 96
Size: 2768 1199 3060
Fragments arranged by size:
3060 2768 1199
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7027
BssSI C'ACGA_G
Cuts at: 3718 5087 6099 3718
Size: 1369 1012 4646
Fragments arranged by size:
4646 1369 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4178 794
Size: 3384 3643
BstBI TT'CG_AA
Cuts at: 5831 5831
Size: 7027
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7027
BstYI r'GATC_y
Cuts at: 1972 2318 3501 4357 4724 5109 5126 6578 6664
Size: 346 1183 856 367 385 17 1452 86
Cuts at: 6664 6676 7022 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1183 856 385 367 346 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3411 3411
Size: 7027
BtgI C'CryG_G
Cuts at: 513 1065 2004 3505 3597 513
Size: 552 939 1501 92 3943
Fragments arranged by size:
3943 1501 939 552 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6288
Fragments arranged by size:
6288 683 56
BtsI GCAGTG_nn'
Cuts at: 4543 5337 5357 4543
Size: 794 20 6213
Fragments arranged by size:
6213 794 20
ClaI AT'CG_AT
Cuts at: 1554 2769 1554
Size: 1215 5812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3579 3614 529
Size: 35 3015 35 3942
Fragments arranged by size:
3942 3015 35 35
DraI TTT'AAA
Cuts at: 4331 4331
Size: 7027
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3023 6034 809
Size: 507 1707 3011 1802
Fragments arranged by size:
3011 1802 1707 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 3681 4474
Size: 54 1425 50 275 18 252 1599 793
Cuts at: 4474 5363 6992 8
Size: 889 1629 43
Fragments arranged by size:
1629 1599 1425 889 793 275 252 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4474 1537
Size: 275 2662 4090
Fragments arranged by size:
4090 2662 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2616 2718 3461 4957 1353
Size: 1263 102 743 1496 3423
Fragments arranged by size:
3423 1496 1263 743 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5614 5988 6134 387
Size: 1064 138 4025 374 146 1280
Fragments arranged by size:
4025 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2147 2198 2283 2640 2727 3113
Size: 393 464 51 51 85 357 87 386
Cuts at: 3113 3268 3605 3652 4199 5090 5687 6474 6742
Size: 155 337 47 547 891 597 787 268
Cuts at: 6742 1239
Size: 1524
Fragments arranged by size:
1524 891 787 597 547 464 393 386
357 337 268 155 87 85 51 51
47
EcoNI CCTnn'n_nnAGG
Cuts at: 3092 3092
Size: 7027
EcoO109I rG'GnC_Cy
Cuts at: 2562 3157 3265 4082 4219 2562
Size: 595 108 817 137 5370
Fragments arranged by size:
5370 817 595 137 108
EcoRI G'AATT_C
Cuts at: 2408 4336 4714 2408
Size: 1928 378 4721
Fragments arranged by size:
4721 1928 378
EcoRV GAT'ATC
Cuts at: 3396 3396
Size: 7027
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3331 3331
Size: 7027
FspI TGC'GCA
Cuts at: 5533 5533
Size: 7027
HaeII r_GCGC'y
Cuts at: 1678 6174 1678
Size: 4496 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2091 2123 2593 2625 3113
Size: 32 135 32 519 32 470 32 488
Cuts at: 3113 3145 3612 3644 5668 5700 5742 5774 1373
Size: 32 467 32 2024 32 42 32 2626
Fragments arranged by size:
2626 2024 519 488 470 467 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2239 2689 4371 4617 669
Size: 988 281 301 450 1682 246 3079
Fragments arranged by size:
3079 1682 988 450 301 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7027
HpaI GTT'AAC
Cuts at: 4617 4617
Size: 7027
KpnI G_GTAC'C
Cuts at: 2153 4352 2153
Size: 2199 4828
MfeI C'AATT_G
Cuts at: 4626 4626
Size: 7027
MluI A'CGCG_T
Cuts at: 6903 6903
Size: 7027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3172 4224 6141 6325 1305
Size: 1867 1052 1917 184 2007
Fragments arranged by size:
2007 1917 1867 1052 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2084 3683 10
Size: 54 1425 343 252 1599 3354
Fragments arranged by size:
3354 1599 1425 343 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3485 4148 4985 5344 5503 518
Size: 648 2319 663 837 359 159 2042
Fragments arranged by size:
2319 2042 837 663 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2379 3803 4443 5120 6268 6513 1606
Size: 773 1424 640 677 1148 245 2120
Fragments arranged by size:
2120 1424 1148 773 677 640 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5536
Fragments arranged by size:
5536 939 552
NdeI CA'TA_TG
Cuts at: 387 3814 387
Size: 3427 3600
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7027
NotI GC'GGCC_GC
Cuts at: 4474 4474
Size: 7027
NsiI A_TGCA'T
Cuts at: 3492 3492
Size: 7027
NspI r_CATG'y
Cuts at: 1132 1229 2404 2766 3787 4385 5930 1132
Size: 97 1175 362 1021 598 1545 2229
Fragments arranged by size:
2229 1545 1175 1021 598 362 97
PasI CC'CwG_GG
Cuts at: 1348 3246 1348
Size: 1898 5129
PciI A'CATG_T
Cuts at: 2762 5926 2762
Size: 3164 3863
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3434 3818 1108
Size: 2326 384 4317
Fragments arranged by size:
4317 2326 384
PfoI T'CCnGG_A
Cuts at: 4215 4215
Size: 7027
PmeI GTTT'AAAC
Cuts at: 4331 4331
Size: 7027
PmlI CAC'GTG
Cuts at: 2958 2958
Size: 7027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5096 5128 6609 6641 5096
Size: 32 1481 32 5482
Fragments arranged by size:
5482 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2562 3157 3265 4082 4219 2562
Size: 595 108 817 137 5370
Fragments arranged by size:
5370 817 595 137 108
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7027
PsiI TTA'TAA
Cuts at: 3612 4597 3612
Size: 985 6042
PstI C_TGCA'G
Cuts at: 830 2388 2671 3156 4379 830
Size: 1558 283 485 1223 3478
Fragments arranged by size:
3478 1558 1223 485 283
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7027
PvuII CAG'CTG
Cuts at: 3803 3803
Size: 7027
SalI G'TCGA_C
Cuts at: 1936 4369 1936
Size: 2433 4594
SapI GCTCTTCn'nnn_
Cuts at: 2718 3461 2718
Size: 743 6284
SbfI CC_TGCA'GG
Cuts at: 4379 4379
Size: 7027
ScaI AGT'ACT
Cuts at: 3454 5275 6987 3454
Size: 1821 1712 3494
Fragments arranged by size:
3494 1821 1712
SfcI C'TryA_G
Cuts at: 826 1045 2384 2667 3152 3964 4223 4375 5510
Size: 219 1339 283 485 812 259 152 1135
Cuts at: 5510 6191 6382 826
Size: 681 191 1471
Fragments arranged by size:
1471 1339 1135 812 681 485 283 259
219 191 152
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7027
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7027
SmaI CCC'GGG
Cuts at: 1872 4354 1872
Size: 2482 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5131 6032 6294 820
Size: 197 926 3188 901 262 1553
Fragments arranged by size:
3188 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4818 493
Size: 4325 2702
SpeI A'CTAG_T
Cuts at: 152 3059 152
Size: 2907 4120
SphI G_CATG'C
Cuts at: 1132 2404 4385 1132
Size: 1272 1981 3774
Fragments arranged by size:
3774 1981 1272
SspI AAT'ATT
Cuts at: 5 52 4951 5
Size: 47 4899 2081
Fragments arranged by size:
4899 2081 47
StuI AGG'CCT
Cuts at: 1517 3416 3970 1517
Size: 1899 554 4574
Fragments arranged by size:
4574 1899 554
StyI C'CwwG_G
Cuts at: 513 1065 2004 2092 3837 3900 4466 513
Size: 552 939 88 1745 63 566 3074
Fragments arranged by size:
3074 1745 939 566 552 88 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4244 5054 5071 5224 5409 5854 325
Size: 1255 2664 810 17 153 185 445 1498
Fragments arranged by size:
2664 1498 1255 810 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2864 3452 3477
Size: 274 80 33 51 1443 887 588 25
Cuts at: 3477 4063 4154 5273 6985 96
Size: 586 91 1119 1712 138
Fragments arranged by size:
1712 1443 1119 887 588 586 274 138
91 80 51 33 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2196 2228 2500 2532 4024 4056 6987 7019 2196
Size: 32 272 32 1492 32 2931 32 2204
Fragments arranged by size:
2931 2204 1492 272 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3020 1316
Size: 1704 5323
XbaI T'CTAG_A
Cuts at: 4363 4363
Size: 7027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3763 3763
Size: 7027
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7027
XmaI C'CCGG_G
Cuts at: 1870 4352 1870
Size: 2482 4545
XmnI GAAnn'nnTTC
Cuts at: 5156 5156
Size: 7027
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3623 4835 276
Size: 53 83 186 936 2089 1212 2468
Fragments arranged by size:
2468 2089 1212 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI