Restriction map for
pFN21AE3294
(Circular) (Six-base) MAPSORT of: pFN21AE3294.seq Check: 7239 from: 1 to: 7003
pFN21AE3294 7003 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2495 3058 2495
Size: 563 6440
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4813 278
Size: 53 83 186 936 3277 2468
Fragments arranged by size:
3277 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3534 4140 4346 1937
Size: 1597 606 206 4594
Fragments arranged by size:
4594 1597 606 206
Acc65I G'GTAC_C
Cuts at: 4324 4324
Size: 7003
AclI AA'CG_TT
Cuts at: 1563 5130 5503 1563
Size: 3567 373 3063
Fragments arranged by size:
3567 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2200 2368 2509 2990 3206 3382 3608 3758
Size: 568 168 141 481 216 176 226 150
Cuts at: 3758 3838 5066 6450 6718 1632
Size: 80 1228 1384 268 1917
Fragments arranged by size:
1917 1384 1228 568 481 268 226 216
176 168 150 141 80
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6806
AflIII A'CryG_T
Cuts at: 1203 3620 4293 5902 6879 1203
Size: 2417 673 1609 977 1327
Fragments arranged by size:
2417 1609 1327 977 673
AgeI A'CCGG_T
Cuts at: 5834 5834
Size: 7003
AhdI GACnn_n'nnGTC
Cuts at: 5732 5732
Size: 7003
AleI CACnn'nnGTG
Cuts at: 1166 2265 2396 1166
Size: 1099 131 5773
Fragments arranged by size:
5773 1099 131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3188 1184
Size: 2004 4999
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2834 2866 4047 4079 2834
Size: 32 1181 32 5758
Fragments arranged by size:
5758 1181 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2293 2317 2374 2497 2836 3073 3175 3763 4746
Size: 24 57 123 339 237 102 588 983
Cuts at: 4746 6318 2293
Size: 1572 2978
Fragments arranged by size:
2978 1572 983 588 339 237 123 102
57 24
ApaI G_GGCC'C
Cuts at: 3184 4120 3184
Size: 936 6067
ApaLI G'TGCA_C
Cuts at: 3503 5060 6216 3503
Size: 1557 1156 4290
Fragments arranged by size:
4290 1557 1156
ApoI r'AATT_y
Cuts at: 1493 3271 3419 3743 4312 4538 4690 6799 1493
Size: 1778 148 324 569 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 569 324 226 152 148
AseI AT'TA_AT
Cuts at: 160 1032 5557 160
Size: 872 4525 1606
Fragments arranged by size:
4525 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7003
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2766 2805 2990 3407 3941 4328
Size: 73 78 745 39 185 417 534 387
Cuts at: 4328 1870
Size: 4545
Fragments arranged by size:
4545 745 534 417 387 185 78 73
39
BamHI G'GATC_C
Cuts at: 2256 4333 4700 2256
Size: 2077 367 4559
Fragments arranged by size:
4559 2077 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 4324 5779 618
Size: 325 217 627 285 2252 1455 1842
Fragments arranged by size:
2252 1842 1455 627 325 285 217
BanII G_rGCy'C
Cuts at: 1391 2968 2997 3184 3447 3966 4120 4322 1391
Size: 1577 29 187 263 519 154 202 4072
Fragments arranged by size:
4072 1577 519 263 202 187 154 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3844 928
Size: 971 1945 4087
Fragments arranged by size:
4087 1945 971
BbvCI CC'TCA_GC
Cuts at: 3815 3815
Size: 7003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5194 5228 1903
Size: 34 3257 34 3678
Fragments arranged by size:
3678 3257 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4895 6105 4895
Size: 1210 5793
BclI T'GATC_A
Cuts at: 1548 4167 1548
Size: 2619 4384
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2415 3086 5614
Size: 107 122 71 631 605 743 671 2528
Cuts at: 5614 136
Size: 1525
Fragments arranged by size:
2528 1525 743 671 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2139 6998 2139
Size: 4859 2144
BlpI GC'TnA_GC
Cuts at: 2816 3452 4420 2816
Size: 636 968 5399
Fragments arranged by size:
5399 968 636
Bme1580I G_kGCm'C
Cuts at: 1790 3184 3507 3655 4120 5064 6220 1790
Size: 1394 323 148 465 944 1156 2573
Fragments arranged by size:
2573 1394 1156 944 465 323 148
BmgBI CAC'GTC
Cuts at: 1318 4296 1318
Size: 2978 4025
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2653 3832 4114
Size: 416 53 83 399 24 1236 1179 282
Cuts at: 4114 4734 5692 442
Size: 620 958 1753
Fragments arranged by size:
1753 1236 1179 958 620 416 399 282
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7003
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 3133 3301 2792
Size: 341 168 6494
Fragments arranged by size:
6494 341 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2764 3667 5663 1239
Size: 1076 449 903 1996 2579
Fragments arranged by size:
2579 1996 1076 903 449
Bpu10I CC'TnA_GC
Cuts at: 3815 3815
Size: 7003
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2425 3191 5128 5993 6291 2425
Size: 766 1937 865 298 3137
Fragments arranged by size:
3137 1937 865 766 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2093 2763 4223 5666 882
Size: 617 594 670 1460 1443 2219
Fragments arranged by size:
2219 1460 1443 670 617 594
BsaAI yAC'GTr
Cuts at: 493 1204 4794 493
Size: 711 3590 2702
Fragments arranged by size:
3590 2702 711
BsaBI GATnn'nnATC
Cuts at: 6997 6997
Size: 7003
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3912 3954 4810
Size: 53 83 186 936 476 1903 42 856
Cuts at: 4810 5192 5827 275
Size: 382 635 1451
Fragments arranged by size:
1903 1451 936 856 635 476 382 186
83 53 42
BsaWI w'CCGG_w
Cuts at: 2116 5436 5834 6108 6255 6666 2116
Size: 3320 398 274 147 411 2453
Fragments arranged by size:
3320 2453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2762 2792 4047 4077 1178
Size: 30 165 30 1359 30 1255 30 4104
Fragments arranged by size:
4104 1359 1255 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2392 2453 2456 3425 3989 4295 1186
Size: 1206 61 3 969 564 306 3894
Fragments arranged by size:
3894 1206 969 564 306 61 3
BseYI C'CCAG_C
Cuts at: 2132 2836 3966 4011 6206 6702 2132
Size: 704 1130 45 2195 496 2433
Fragments arranged by size:
2433 2195 1130 704 496 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2300 2723 2753 2849 3386 1163
Size: 573 564 423 30 96 537 4780
Fragments arranged by size:
4780 573 564 537 423 96 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2594 4218 4453 5214 6242 1540
Size: 275 186 593 1624 235 761 1028 2301
Fragments arranged by size:
2301 1624 1028 761 593 275 235 186
BsiHKAI G_wGCw'C
Cuts at: 3102 3447 3507 4263 5064 5149 6220 3102
Size: 345 60 756 801 85 1071 3885
Fragments arranged by size:
3885 1071 801 756 345 85 60
BsmI GAATG_Cn'
Cuts at: 2628 3082 4514 4607 2628
Size: 454 1432 93 5024
Fragments arranged by size:
5024 1432 454 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2968 2997 3102 3184 3447 3507 3655
Size: 399 1178 29 105 82 263 60 148
Cuts at: 3655 3966 4120 4263 4322 5064 5149 6220 1391
Size: 311 154 143 59 742 85 1071 2174
Fragments arranged by size:
2174 1178 1071 742 399 311 263 154
148 143 105 85 82 60 59 29
BspEI T'CCGG_A
Cuts at: 2116 6666 2116
Size: 4550 2453
BspHI T'CATG_A
Cuts at: 4892 6622 4892
Size: 1730 5273
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2094 2495 3058 3304 4358 844
Size: 1250 401 563 246 1054 3489
Fragments arranged by size:
3489 1250 1054 563 401 246
BsrBI CCG'CTC
Cuts at: 1394 1823 3254 3285 4890 1394
Size: 429 1431 31 1605 3507
Fragments arranged by size:
3507 1605 1431 429 31
BsrDI GCAATG_nn'
Cuts at: 65 1248 3520 5498 5672 65
Size: 1183 2272 1978 174 1396
Fragments arranged by size:
2272 1978 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2148 3102 3309 5647 5834 1700
Size: 110 338 954 207 2338 187 2869
Fragments arranged by size:
2869 2338 954 338 207 187 110
BsrGI T'GTAC_A
Cuts at: 96 2420 96
Size: 2324 4679
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7003
BssSI C'ACGA_G
Cuts at: 2204 4130 5063 6075 2204
Size: 1926 933 1012 3132
Fragments arranged by size:
3132 1926 1012 933
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2746 3763 794
Size: 1952 1017 4034
Fragments arranged by size:
4034 1952 1017
BstBI TT'CG_AA
Cuts at: 5807 5807
Size: 7003
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7003
BstXI CCAn_nnnn'nTGG
Cuts at: 4240 4240
Size: 7003
BstYI r'GATC_y
Cuts at: 1972 2139 2256 4333 4700 5085 5102 6554 6640
Size: 167 117 2077 367 385 17 1452 86
Cuts at: 6640 6652 6998 1972
Size: 12 346 1977
Fragments arranged by size:
2077 1977 1452 385 367 346 167 117
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3555 513
Size: 552 939 1551 3961
Fragments arranged by size:
3961 1551 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3130 487
Size: 683 56 1904 4360
Fragments arranged by size:
4360 1904 683 56
BtsI GCAGTG_nn'
Cuts at: 4261 4519 5313 5333 4261
Size: 258 794 20 5931
Fragments arranged by size:
5931 794 258 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7003
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3028 3063 529
Size: 35 2464 35 4469
Fragments arranged by size:
4469 2464 35 35
DraI TTT'AAA
Cuts at: 4276 4307 4276
Size: 31 6972
DraIII CAC_nnn'GTG
Cuts at: 2661 2661
Size: 7003
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2153 6010 809
Size: 507 837 3857 1802
Fragments arranged by size:
3857 1802 837 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2591 2866 4450
Size: 54 1425 50 275 18 761 275 1584
Cuts at: 4450 5339 6968 8
Size: 889 1629 43
Fragments arranged by size:
1629 1584 1425 889 761 275 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2591 4450 1537
Size: 275 779 1859 4090
Fragments arranged by size:
4090 1859 779 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2417 2901 2966 3057 3263 3728 4933 1353
Size: 1064 484 65 91 206 465 1205 3423
Fragments arranged by size:
3423 1205 1064 484 465 206 91 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2344 4055 5590 5964 6110 387
Size: 1064 138 755 1711 1535 374 146 1280
Fragments arranged by size:
1711 1535 1280 1064 755 374 146 138
EcoICRI GAG'CTC
Cuts at: 3445 3445
Size: 7003
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2200 2315 2368 2509 2764 2990 3206
Size: 393 568 115 53 141 255 226 216
Cuts at: 3206 3382 3608 3667 3758 3838 5066 5663 6450
Size: 176 226 59 91 80 1228 597 787
Cuts at: 6450 6718 1239
Size: 268 1524
Fragments arranged by size:
1524 1228 787 597 568 393 268 255
226 226 216 176 141 115 91 80
59 53
EcoNI CCTnn'n_nnAGG
Cuts at: 2267 2279 4076 2267
Size: 12 1797 5194
Fragments arranged by size:
5194 1797 12
EcoO109I rG'GnC_Cy
Cuts at: 2272 2290 2314 2569 3181 4117 2272
Size: 18 24 255 612 936 5158
Fragments arranged by size:
5158 936 612 255 24 18
EcoRI G'AATT_C
Cuts at: 3419 3743 4312 4690 3419
Size: 324 569 378 5732
Fragments arranged by size:
5732 569 378 324
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3586 3586
Size: 7003
FspI TGC'GCA
Cuts at: 2060 5509 2060
Size: 3449 3554
HaeII r_GCGC'y
Cuts at: 1678 3252 6150 1678
Size: 1574 2898 2531
Fragments arranged by size:
2898 2531 1574
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2336 2368 2762 2794 4007
Size: 32 135 32 764 32 394 32 1213
Cuts at: 4007 4039 5644 5676 5718 5750 1373
Size: 32 1605 32 42 32 2626
Fragments arranged by size:
2626 1605 1213 764 394 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2479 3439 4347 4593 669
Size: 988 281 541 960 908 246 3079
Fragments arranged by size:
3079 988 960 908 541 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7003
HpaI GTT'AAC
Cuts at: 4593 4593
Size: 7003
KpnI G_GTAC'C
Cuts at: 4328 4328
Size: 7003
MfeI C'AATT_G
Cuts at: 4602 4602
Size: 7003
MluI A'CGCG_T
Cuts at: 6879 6879
Size: 7003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 2733 3846 6117 6301 1305
Size: 732 696 1113 2271 184 2007
Fragments arranged by size:
2271 2007 1113 732 696 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2868 10
Size: 54 1425 343 1036 4145
Fragments arranged by size:
4145 1425 1036 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2265 2396 3219 3236 4961 5320 5479
Size: 648 1099 131 823 17 1725 359 159
Cuts at: 5479 518
Size: 2042
Fragments arranged by size:
2042 1725 1099 823 648 359 159 131
17
MspA1I CmG'CkG
Cuts at: 1606 2737 2980 3172 3370 3493 4419 5096 6244
Size: 1131 243 192 198 123 926 677 1148
Cuts at: 6244 6489 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1131 926 677 245 243 198
192 123
NaeI GCC'GGC
Cuts at: 3311 3311
Size: 7003
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5512
Fragments arranged by size:
5512 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7003
NgoMIV G'CCGG_C
Cuts at: 3309 3309
Size: 7003
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7003
NotI GC'GGCC_GC
Cuts at: 2591 4450 2591
Size: 1859 5144
NspI r_CATG'y
Cuts at: 1132 1229 3624 3994 4361 5906 1132
Size: 97 2395 370 367 1545 2229
Fragments arranged by size:
2395 2229 1545 370 367 97
PasI CC'CwG_GG
Cuts at: 1348 2077 2450 4078 1348
Size: 729 373 1628 4273
Fragments arranged by size:
4273 1628 729 373
PciI A'CATG_T
Cuts at: 3620 5902 3620
Size: 2282 4721
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2293 2395 2497 3634 1108
Size: 1185 102 102 1137 4477
Fragments arranged by size:
4477 1185 1137 102 102
PfoI T'CCnGG_A
Cuts at: 3644 3726 3644
Size: 82 6921
PmeI GTTT'AAAC
Cuts at: 4307 4307
Size: 7003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4047 4079 5072 5104 6585 6617 4047
Size: 32 993 32 1481 32 4433
Fragments arranged by size:
4433 1481 993 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2272 2290 2314 2569 2272
Size: 18 24 255 6706
Fragments arranged by size:
6706 255 24 18
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7003
PsiI TTA'TAA
Cuts at: 4573 4573
Size: 7003
PspOMI G'GGCC_C
Cuts at: 3180 4116 3180
Size: 936 6067
PstI C_TGCA'G
Cuts at: 830 2370 2891 2985 4268 4355 830
Size: 1540 521 94 1283 87 3478
Fragments arranged by size:
3478 1540 1283 521 94 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7003
PvuII CAG'CTG
Cuts at: 2737 3172 2737
Size: 435 6568
SacI G_AGCT'C
Cuts at: 3447 3447
Size: 7003
SalI G'TCGA_C
Cuts at: 1936 4345 1936
Size: 2409 4594
SanDI GG'GwC_CC
Cuts at: 2569 2569
Size: 7003
SapI GCTCTTCn'nnn_
Cuts at: 3057 3728 3057
Size: 671 6332
SbfI CC_TGCA'GG
Cuts at: 4355 4355
Size: 7003
ScaI AGT'ACT
Cuts at: 4154 5251 6963 4154
Size: 1097 1712 4194
Fragments arranged by size:
4194 1712 1097
SexAI A'CCwGG_T
Cuts at: 2274 2316 3627 2274
Size: 42 1311 5650
Fragments arranged by size:
5650 1311 42
SfcI C'TryA_G
Cuts at: 826 1045 2366 2887 2981 4264 4351 5486 6167
Size: 219 1321 521 94 1283 87 1135 681
Cuts at: 6167 6358 826
Size: 191 1471
Fragments arranged by size:
1471 1321 1283 1135 681 521 219 191
94 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7003
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7003
SmaI CCC'GGG
Cuts at: 1872 4330 1872
Size: 2458 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2404 3206 5107 6008 6270 820
Size: 197 926 461 802 1901 901 262 1553
Fragments arranged by size:
1901 1553 926 901 802 461 262 197
SnaBI TAC'GTA
Cuts at: 493 4794 493
Size: 4301 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7003
SphI G_CATG'C
Cuts at: 1132 4361 1132
Size: 3229 3774
SspI AAT'ATT
Cuts at: 5 52 4927 5
Size: 47 4875 2081
Fragments arranged by size:
4875 2081 47
StuI AGG'CCT
Cuts at: 1517 3089 3820 3947 1517
Size: 1572 731 127 4573
Fragments arranged by size:
4573 1572 731 127
StyI C'CwwG_G
Cuts at: 513 1065 2004 2613 4233 4442 513
Size: 552 939 609 1620 209 3074
Fragments arranged by size:
3074 1620 939 609 552 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2672 5030 5047 5200 5385 5830 325
Size: 1255 1092 2358 17 153 185 445 1498
Fragments arranged by size:
2358 1498 1255 1092 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2042 2420 4152
Size: 274 80 33 51 1443 65 378 1732
Cuts at: 4152 5249 6961 96
Size: 1097 1712 138
Fragments arranged by size:
1732 1712 1443 1097 378 274 138 80
65 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3013 3045 3977 4009 6963 6995 3013
Size: 32 932 32 2954 32 3021
Fragments arranged by size:
3021 2954 932 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3629 1316
Size: 2313 4690
XbaI T'CTAG_A
Cuts at: 4298 4339 4298
Size: 41 6962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7003
XmaI C'CCGG_G
Cuts at: 1870 4328 1870
Size: 2458 4545
XmnI GAAnn'nnTTC
Cuts at: 3722 3743 4059 5132 3722
Size: 21 316 1073 5593
Fragments arranged by size:
5593 1073 316 21
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4811 276
Size: 53 83 186 936 3277 2468
Fragments arranged by size:
3277 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI
SfoI SrfI SwaI XcmI