Restriction map for
pFN21AE3292
(Circular) (Six-base) MAPSORT of: pFN21AE3292.seq Check: 4564 from: 1 to: 6994
pFN21AE3292 6994 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3154 3154
Size: 6994
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4804 278
Size: 53 83 186 936 3268 2468
Fragments arranged by size:
3268 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3900 4116 4337 1937
Size: 1963 216 221 4594
Fragments arranged by size:
4594 1963 221 216
Acc65I G'GTAC_C
Cuts at: 4315 4315
Size: 6994
AclI AA'CG_TT
Cuts at: 1563 5121 5494 1563
Size: 3558 373 3063
Fragments arranged by size:
3558 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2010 2072 3738 4224 5057 6441 6709 1632
Size: 378 62 1666 486 833 1384 268 1917
Fragments arranged by size:
1917 1666 1384 833 486 378 268 62
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6797
AflIII A'CryG_T
Cuts at: 1203 5893 6870 1203
Size: 4690 977 1327
Fragments arranged by size:
4690 1327 977
AgeI A'CCGG_T
Cuts at: 5825 5825
Size: 6994
AhdI GACnn_n'nnGTC
Cuts at: 3666 5723 3666
Size: 2057 4937
AleI CACnn'nnGTG
Cuts at: 1166 3618 1166
Size: 2452 4542
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2837 3708 1184
Size: 1653 871 4470
Fragments arranged by size:
4470 1653 871
AlwNI CAG_nnn'CTG
Cuts at: 2273 3716 4061 4187 4737 6309 2273
Size: 1443 345 126 550 1572 2958
Fragments arranged by size:
2958 1572 1443 550 345 126
ApaLI G'TGCA_C
Cuts at: 2812 5051 6207 2812
Size: 2239 1156 3599
Fragments arranged by size:
3599 2239 1156
ApoI r'AATT_y
Cuts at: 1493 3405 4303 4529 4681 6790 1493
Size: 1912 898 226 152 2109 1697
Fragments arranged by size:
2109 1912 1697 898 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2325 5548 160
Size: 872 1293 3223 1606
Fragments arranged by size:
3223 1606 1293 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
AvaI C'yCGr_G
Cuts at: 1870 1943 3044 4319 1870
Size: 73 1101 1275 4545
Fragments arranged by size:
4545 1275 1101 73
BamHI G'GATC_C
Cuts at: 3592 4324 4691 3592
Size: 732 367 5895
Fragments arranged by size:
5895 732 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3092 3521 4315 5770 618
Size: 325 217 627 1305 429 794 1455 1842
Fragments arranged by size:
1842 1455 1305 794 627 429 325 217
BanII G_rGCy'C
Cuts at: 1391 2631 3586 4313 1391
Size: 1240 955 727 4072
Fragments arranged by size:
4072 1240 955 727
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2362 3230 3507 3972 928
Size: 971 463 868 277 465 3950
Fragments arranged by size:
3950 971 868 465 463 277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3590 3624 5185 5219 1903
Size: 34 50 34 1569 34 1561 34 3678
Fragments arranged by size:
3678 1569 1561 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4886 6096 4886
Size: 1210 5784
BclI T'GATC_A
Cuts at: 1548 2304 1548
Size: 756 6238
BfrBI ATG'CAT
Cuts at: 2008 3794 2008
Size: 1786 5208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3644 5605 136
Size: 107 122 71 631 605 1972 1961 1525
Fragments arranged by size:
1972 1961 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6989 6989
Size: 6994
BlpI GC'TnA_GC
Cuts at: 4411 4411
Size: 6994
Bme1580I G_kGCm'C
Cuts at: 1790 2816 3524 3627 5055 6211 1790
Size: 1026 708 103 1428 1156 2573
Fragments arranged by size:
2573 1428 1156 1026 708 103
BmgBI CAC'GTC
Cuts at: 1318 3317 1318
Size: 1999 4995
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3014 4049 4725
Size: 416 53 83 399 24 1597 1035 676
Cuts at: 4725 5683 442
Size: 958 1753
Fragments arranged by size:
1753 1597 1035 958 676 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4290 1056
Size: 3234 3760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2823 2976 3093 3514 5654 1239
Size: 1584 153 117 421 2140 2579
Fragments arranged by size:
2579 2140 1584 421 153 117
Bpu10I CC'TnA_GC
Cuts at: 3547 3547
Size: 6994
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2645 5119 5984 6282 2645
Size: 2474 865 298 3357
Fragments arranged by size:
3357 2474 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2779 2994 5657 882
Size: 617 1280 215 2663 2219
Fragments arranged by size:
2663 2219 1280 617 215
BsaAI yAC'GTr
Cuts at: 493 1204 4785 493
Size: 711 3581 2702
Fragments arranged by size:
3581 2702 711
BsaBI GATnn'nnATC
Cuts at: 6988 6988
Size: 6994
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4801 5183 5818 275
Size: 53 83 186 936 3268 382 635 1451
Fragments arranged by size:
3268 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3882 5427 5825 6099 6246 6657 3882
Size: 1545 398 274 147 411 4219
Fragments arranged by size:
4219 1545 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3706 3736 4251 4281 1178
Size: 30 165 30 2303 30 515 30 3891
Fragments arranged by size:
3891 2303 515 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2031 3687 3913 4003 1186
Size: 845 1656 226 90 4177
Fragments arranged by size:
4177 1656 845 226 90
BseYI C'CCAG_C
Cuts at: 2266 3320 6197 6693 2266
Size: 1054 2877 496 2567
Fragments arranged by size:
2877 2567 1054 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2802 3662 4173 1163
Size: 573 1066 860 511 3984
Fragments arranged by size:
3984 1066 860 573 511
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3342 3590 4444 5205 6233 1540
Size: 275 186 1341 248 854 761 1028 2301
Fragments arranged by size:
2301 1341 1028 854 761 275 248 186
BsiHKAI G_wGCw'C
Cuts at: 2631 2816 5055 5140 6211 2631
Size: 185 2239 85 1071 3414
Fragments arranged by size:
3414 2239 1071 185 85
BsmI GAATG_Cn'
Cuts at: 3015 3796 4505 4598 3015
Size: 781 709 93 5411
Fragments arranged by size:
5411 781 709 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2370 2370
Size: 6994
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2631 2816 3524 3586 3627 4313 5055
Size: 399 841 185 708 62 41 686 742
Cuts at: 5055 5140 6211 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 841 742 708 686 399 185
85 62 41
BspEI T'CCGG_A
Cuts at: 6657 6657
Size: 6994
BspHI T'CATG_A
Cuts at: 2631 4883 6613 2631
Size: 2252 1730 3012
Fragments arranged by size:
3012 2252 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3154 4349 844
Size: 2310 1195 3489
Fragments arranged by size:
3489 2310 1195
BsrBI CCG'CTC
Cuts at: 1394 1823 4881 1394
Size: 429 3058 3507
Fragments arranged by size:
3507 3058 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3794 5489 5663 65
Size: 1183 2546 1695 174 1396
Fragments arranged by size:
2546 1695 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5638 5825 1700
Size: 110 3828 187 2869
Fragments arranged by size:
3828 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6994
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6994
BssSI C'ACGA_G
Cuts at: 5054 6066 5054
Size: 1012 5982
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6994
BstBI TT'CG_AA
Cuts at: 2695 5798 2695
Size: 3103 3891
BstEII G'GTnAC_C
Cuts at: 1184 3878 4022 1184
Size: 2694 144 4156
Fragments arranged by size:
4156 2694 144
BstXI CCAn_nnnn'nTGG
Cuts at: 2081 3860 4139 2081
Size: 1779 279 4936
Fragments arranged by size:
4936 1779 279
BstYI r'GATC_y
Cuts at: 1972 3592 4324 4691 5076 5093 6545 6631 6643
Size: 1620 732 367 385 17 1452 86 12
Cuts at: 6643 6989 1972
Size: 346 1977
Fragments arranged by size:
1977 1620 1452 732 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3498 3648 3808 3859 513
Size: 552 2433 150 160 51 3648
Fragments arranged by size:
3648 2433 552 160 150 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6255
Fragments arranged by size:
6255 683 56
BtsI GCAGTG_nn'
Cuts at: 2814 2819 2911 4510 5304 5324 2814
Size: 5 92 1599 794 20 4484
Fragments arranged by size:
4484 1599 794 92 20 5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6994
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6959
DraI TTT'AAA
Cuts at: 4298 4298
Size: 6994
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3976 6001 809
Size: 507 2660 2025 1802
Fragments arranged by size:
2660 2025 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3949 4441 5330
Size: 54 1425 50 275 18 2119 492 889
Cuts at: 5330 6959 8
Size: 1629 43
Fragments arranged by size:
2119 1629 1425 889 492 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4441 1537
Size: 275 2629 4090
Fragments arranged by size:
4090 2629 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4236 4924 1353
Size: 2883 688 3423
Fragments arranged by size:
3423 2883 688
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2257 5581 5955 6101 387
Size: 1064 138 668 3324 374 146 1280
Fragments arranged by size:
3324 1280 1064 668 374 146 138
EcoICRI GAG'CTC
Cuts at: 2629 2629
Size: 6994
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2010 2072 2823 2976 3093 3514 3738
Size: 393 378 62 751 153 117 421 224
Cuts at: 3738 4224 5057 5654 6441 6709 1239
Size: 486 833 597 787 268 1524
Fragments arranged by size:
1524 833 787 751 597 486 421 393
378 268 224 153 117 62
EcoO109I rG'GnC_Cy
Cuts at: 3302 4184 3302
Size: 882 6112
EcoRI G'AATT_C
Cuts at: 3405 4303 4681 3405
Size: 898 378 5718
Fragments arranged by size:
5718 898 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2636 2636
Size: 6994
FspI TGC'GCA
Cuts at: 2802 5500 2802
Size: 2698 4296
HaeII r_GCGC'y
Cuts at: 1678 6141 1678
Size: 4463 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3023 3055 3652 3684 5635
Size: 32 135 32 1451 32 597 32 1951
Cuts at: 5635 5667 5709 5741 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1951 1451 597 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2299 2923 4338 4584 669
Size: 988 281 361 624 1415 246 3079
Fragments arranged by size:
3079 1415 988 624 361 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6994
HpaI GTT'AAC
Cuts at: 4584 4584
Size: 6994
KpnI G_GTAC'C
Cuts at: 4319 4319
Size: 6994
MfeI C'AATT_G
Cuts at: 4593 4593
Size: 6994
MluI A'CGCG_T
Cuts at: 6870 6870
Size: 6994
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3570 3620 3935 4181 6108 6292 1305
Size: 2265 50 315 246 1927 184 2007
Fragments arranged by size:
2265 2007 1927 315 246 184 50
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3951 10
Size: 54 1425 343 2119 3053
Fragments arranged by size:
3053 2119 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2325 2651 3032 3618 3807 3858 4952
Size: 648 1159 326 381 586 189 51 1094
Cuts at: 4952 5311 5470 518
Size: 359 159 2042
Fragments arranged by size:
2042 1159 1094 648 586 381 359 326
189 159 51
MspA1I CmG'CkG
Cuts at: 1606 3452 3635 4410 5087 6235 6480 1606
Size: 1846 183 775 677 1148 245 2120
Fragments arranged by size:
2120 1846 1148 775 677 245 183
NcoI C'CATG_G
Cuts at: 513 1065 3498 3648 3808 3859 513
Size: 552 2433 150 160 51 3648
Fragments arranged by size:
3648 2433 552 160 150 51
NdeI CA'TA_TG
Cuts at: 387 2533 387
Size: 2146 4848
NheI G'CTAG_C
Cuts at: 1052 4286 1052
Size: 3234 3760
NotI GC'GGCC_GC
Cuts at: 4441 4441
Size: 6994
NsiI A_TGCA'T
Cuts at: 2010 3796 2010
Size: 1786 5208
NspI r_CATG'y
Cuts at: 1132 1229 2034 4352 5897 1132
Size: 97 805 2318 1545 2229
Fragments arranged by size:
2318 2229 1545 805 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6994
PciI A'CATG_T
Cuts at: 5893 5893
Size: 6994
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6994
PmeI GTTT'AAAC
Cuts at: 4298 4298
Size: 6994
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5063 5095 6576 6608 5063
Size: 32 1481 32 5449
Fragments arranged by size:
5449 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3302 4184 3302
Size: 882 6112
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6994
PsiI TTA'TAA
Cuts at: 4564 4564
Size: 6994
PstI C_TGCA'G
Cuts at: 830 3429 4346 830
Size: 2599 917 3478
Fragments arranged by size:
3478 2599 917
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6994
PvuII CAG'CTG
Cuts at: 3452 3635 3452
Size: 183 6811
SacI G_AGCT'C
Cuts at: 2631 2631
Size: 6994
SalI G'TCGA_C
Cuts at: 1936 4336 1936
Size: 2400 4594
SbfI CC_TGCA'GG
Cuts at: 4346 4346
Size: 6994
ScaI AGT'ACT
Cuts at: 5242 6954 5242
Size: 1712 5282
SexAI A'CCwGG_T
Cuts at: 2208 3304 2208
Size: 1096 5898
SfcI C'TryA_G
Cuts at: 826 1045 3425 4281 4342 5477 6158 6349 826
Size: 219 2380 856 61 1135 681 191 1471
Fragments arranged by size:
2380 1471 1135 856 681 219 191 61
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6994
SmaI CCC'GGG
Cuts at: 1872 4321 1872
Size: 2449 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2624 5098 5999 6261 820
Size: 197 926 681 2474 901 262 1553
Fragments arranged by size:
2474 1553 926 901 681 262 197
SnaBI TAC'GTA
Cuts at: 493 4785 493
Size: 4292 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6994
SphI G_CATG'C
Cuts at: 1132 2034 4352 1132
Size: 902 2318 3774
Fragments arranged by size:
3774 2318 902
SspI AAT'ATT
Cuts at: 5 52 4918 5
Size: 47 4866 2081
Fragments arranged by size:
4866 2081 47
StuI AGG'CCT
Cuts at: 1517 4040 1517
Size: 2523 4471
StyI C'CwwG_G
Cuts at: 513 1065 3498 3648 3808 3859 3873 3893 4027
Size: 552 2433 150 160 51 14 20 134
Cuts at: 4027 4071 4433 513
Size: 44 362 3074
Fragments arranged by size:
3074 2433 552 362 160 150 134 51
44 20 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2976 5021 5038 5191 5376 5821 325
Size: 1255 1396 2045 17 153 185 445 1498
Fragments arranged by size:
2045 1498 1396 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2121 3246 3533
Size: 274 80 33 51 1443 144 1125 287
Cuts at: 3533 5240 6952 96
Size: 1707 1712 138
Fragments arranged by size:
1712 1707 1443 1125 287 274 144 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2811 2843 3706 3738 4251 4283 6954 6986 2811
Size: 32 863 32 513 32 2671 32 2819
Fragments arranged by size:
2819 2671 863 513 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6994
XbaI T'CTAG_A
Cuts at: 4330 4330
Size: 6994
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3207 3207
Size: 6994
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6994
XmaI C'CCGG_G
Cuts at: 1870 4319 1870
Size: 2449 4545
XmnI GAAnn'nnTTC
Cuts at: 2290 5123 2290
Size: 2833 4161
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4802 276
Size: 53 83 186 936 3268 2468
Fragments arranged by size:
3268 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI