Restriction map for
pFN21AE3289
(Circular) (Six-base) MAPSORT of: pFN21AE3289.seq Check: 6222 from: 1 to: 6940
pFN21AE3289 6940 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4750 278
Size: 53 83 186 936 3214 2468
Fragments arranged by size:
3214 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2050 3114 4283 1937
Size: 113 1064 1169 4594
Fragments arranged by size:
4594 1169 1064 113
Acc65I G'GTAC_C
Cuts at: 3534 4261 3534
Size: 727 6213
AclI AA'CG_TT
Cuts at: 1563 5067 5440 1563
Size: 3504 373 3063
Fragments arranged by size:
3504 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3423 3429 3880 3941 5003 6387 6655 1632
Size: 1791 6 451 61 1062 1384 268 1917
Fragments arranged by size:
1917 1791 1384 1062 451 268 61 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6743
AflIII A'CryG_T
Cuts at: 1203 5839 6816 1203
Size: 4636 977 1327
Fragments arranged by size:
4636 1327 977
AgeI A'CCGG_T
Cuts at: 5771 5771
Size: 6940
AhdI GACnn_n'nnGTC
Cuts at: 5669 5669
Size: 6940
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3891 1184
Size: 2707 4233
AlwNI CAG_nnn'CTG
Cuts at: 4683 6255 4683
Size: 1572 5368
ApaLI G'TGCA_C
Cuts at: 2544 4997 6153 2544
Size: 2453 1156 3331
Fragments arranged by size:
3331 2453 1156
ApoI r'AATT_y
Cuts at: 1493 2908 3596 4012 4082 4249 4475 4627 6736
Size: 1415 688 416 70 167 226 152 2109
Cuts at: 6736 1493
Size: 1697
Fragments arranged by size:
2109 1697 1415 688 416 226 167 152
70
AseI AT'TA_AT
Cuts at: 160 1032 5494 160
Size: 872 4462 1606
Fragments arranged by size:
4462 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6940
AvaI C'yCGr_G
Cuts at: 1870 1943 4265 1870
Size: 73 2322 4545
Fragments arranged by size:
4545 2322 73
BamHI G'GATC_C
Cuts at: 4270 4637 4270
Size: 367 6573
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3534 3666 4261 5716 618
Size: 325 217 627 1747 132 595 1455 1842
Fragments arranged by size:
1842 1747 1455 627 595 325 217 132
BanII G_rGCy'C
Cuts at: 1391 4259 1391
Size: 2868 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3842 928
Size: 971 1943 4026
Fragments arranged by size:
4026 1943 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2163 2197 2326 2360 5131 5165 1903
Size: 34 226 34 129 34 2771 34 3678
Fragments arranged by size:
3678 2771 226 129 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2461 3931 4011 4832 6042 2461
Size: 1470 80 821 1210 3359
Fragments arranged by size:
3359 1470 1210 821 80
BclI T'GATC_A
Cuts at: 1548 3279 3454 1548
Size: 1731 175 5034
Fragments arranged by size:
5034 1731 175
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5551 136
Size: 107 122 71 631 605 3879 1525
Fragments arranged by size:
3879 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3274 6935 3274
Size: 3661 3279
BlpI GC'TnA_GC
Cuts at: 4357 4357
Size: 6940
Bme1580I G_kGCm'C
Cuts at: 1790 2548 3671 5001 6157 1790
Size: 758 1123 1330 1156 2573
Fragments arranged by size:
2573 1330 1156 1123 758
BmgBI CAC'GTC
Cuts at: 1318 3127 1318
Size: 1809 5131
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2776 4671 5629
Size: 416 53 83 399 24 1359 1895 958
Cuts at: 5629 442
Size: 1753
Fragments arranged by size:
1895 1753 1359 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6940
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3300 5600 1239
Size: 2061 2300 2579
Fragments arranged by size:
2579 2300 2061
Bpu10I CC'TnA_GC
Cuts at: 4097 4097
Size: 6940
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5065 5930 6228 5065
Size: 865 298 5777
Fragments arranged by size:
5777 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5603 882
Size: 617 4104 2219
Fragments arranged by size:
4104 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4731 493
Size: 711 3527 2702
Fragments arranged by size:
3527 2702 711
BsaBI GATnn'nnATC
Cuts at: 2148 3357 6934 2148
Size: 1209 3577 2154
Fragments arranged by size:
3577 2154 1209
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4747 5129 5764 275
Size: 53 83 186 936 3214 382 635 1451
Fragments arranged by size:
3214 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2313 5373 5771 6045 6192 6603 2313
Size: 3060 398 274 147 411 2650
Fragments arranged by size:
3060 2650 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2940 2970 1178
Size: 30 165 30 1537 30 5148
Fragments arranged by size:
5148 1537 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2027 2741 1186
Size: 838 3 714 5385
Fragments arranged by size:
5385 838 714 3
BseYI C'CCAG_C
Cuts at: 6143 6639 6143
Size: 496 6444
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2139 1163
Size: 573 403 5964
Fragments arranged by size:
5964 573 403
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4390 5151 6179 1540
Size: 275 186 2389 761 1028 2301
Fragments arranged by size:
2389 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2548 5001 5086 6157 2548
Size: 2453 85 1071 3331
Fragments arranged by size:
3331 2453 1071 85
BsmI GAATG_Cn'
Cuts at: 2023 2782 4451 4544 2023
Size: 759 1669 93 4419
Fragments arranged by size:
4419 1669 759 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2548 3671 4259 5001 5086 6157 1391
Size: 399 758 1123 588 742 85 1071 2174
Fragments arranged by size:
2174 1123 1071 758 742 588 399 85
BspEI T'CCGG_A
Cuts at: 6603 6603
Size: 6940
BspHI T'CATG_A
Cuts at: 4829 6559 4829
Size: 1730 5210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4295 844
Size: 3451 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4827 1394
Size: 429 3004 3507
Fragments arranged by size:
3507 3004 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3290 5435 5609 65
Size: 1183 2042 2145 174 1396
Fragments arranged by size:
2145 2042 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5584 5771 1700
Size: 110 3774 187 2869
Fragments arranged by size:
3774 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6940
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6940
BssSI C'ACGA_G
Cuts at: 3963 4185 5000 6012 3963
Size: 222 815 1012 4891
Fragments arranged by size:
4891 1012 815 222
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6940
BstBI TT'CG_AA
Cuts at: 5744 5744
Size: 6940
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6940
BstXI CCAn_nnnn'nTGG
Cuts at: 2581 2581
Size: 6940
BstYI r'GATC_y
Cuts at: 1972 3250 3274 4270 4637 5022 5039 6491 6577
Size: 1278 24 996 367 385 17 1452 86
Cuts at: 6577 6589 6935 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1278 996 385 367 346 86
24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2214 513
Size: 552 939 210 5239
Fragments arranged by size:
5239 939 552 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6201
Fragments arranged by size:
6201 683 56
BtsI GCAGTG_nn'
Cuts at: 3989 4456 5250 5270 3989
Size: 467 794 20 5659
Fragments arranged by size:
5659 794 467 20
ClaI AT'CG_AT
Cuts at: 1554 2290 1554
Size: 736 6204
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6905
DraI TTT'AAA
Cuts at: 4227 4244 4227
Size: 17 6923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2160 5947 809
Size: 507 844 3787 1802
Fragments arranged by size:
3787 1802 844 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2294 4387 5276
Size: 54 1425 50 275 18 464 2093 889
Cuts at: 5276 6905 8
Size: 1629 43
Fragments arranged by size:
2093 1629 1425 889 464 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4387 1537
Size: 275 2575 4090
Fragments arranged by size:
4090 2575 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2385 4870 1353
Size: 1032 2485 3423
Fragments arranged by size:
3423 2485 1032
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5527 5901 6047 387
Size: 1064 138 3938 374 146 1280
Fragments arranged by size:
3938 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3300 3423 3429 3880 3941 5003 5600
Size: 393 1668 123 6 451 61 1062 597
Cuts at: 5600 6387 6655 1239
Size: 787 268 1524
Fragments arranged by size:
1668 1524 1062 787 597 451 393 268
123 61 6
EcoO109I rG'GnC_Cy
Cuts at: 3862 3862
Size: 6940
EcoRI G'AATT_C
Cuts at: 4249 4627 4249
Size: 378 6562
EcoRV GAT'ATC
Cuts at: 2599 2599
Size: 6940
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2735 2735
Size: 6940
FspI TGC'GCA
Cuts at: 5446 5446
Size: 6940
HaeII r_GCGC'y
Cuts at: 1678 6087 1678
Size: 4409 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2086 2118 2303 2335 2435
Size: 32 135 32 514 32 185 32 100
Cuts at: 2435 2454 2467 2486 2519 2551 3131 3163 5581
Size: 19 13 19 33 32 580 32 2418
Cuts at: 5581 5613 5655 5687 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2418 580 514 185 135 100 42
33 32 32 32 32 32 32 32
32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 4284 4530 669
Size: 988 281 2346 246 3079
Fragments arranged by size:
3079 2346 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2683 748
Size: 1935 5005
HpaI GTT'AAC
Cuts at: 4530 4530
Size: 6940
KpnI G_GTAC'C
Cuts at: 3538 4265 3538
Size: 727 6213
MfeI C'AATT_G
Cuts at: 2360 4539 2360
Size: 2179 4761
MluI A'CGCG_T
Cuts at: 6816 6816
Size: 6940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3083 6054 6238 1305
Size: 1778 2971 184 2007
Fragments arranged by size:
2971 2007 1778 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2296 10
Size: 54 1425 343 464 4654
Fragments arranged by size:
4654 1425 464 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2426 2508 2627 2721 4174 4898 5257
Size: 648 1260 82 119 94 1453 724 359
Cuts at: 5257 5416 518
Size: 159 2042
Fragments arranged by size:
2042 1453 1260 724 648 359 159 119
94 82
MspA1I CmG'CkG
Cuts at: 1606 4356 5033 6181 6426 1606
Size: 2750 677 1148 245 2120
Fragments arranged by size:
2750 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2214 513
Size: 552 939 210 5239
Fragments arranged by size:
5239 939 552 210
NdeI CA'TA_TG
Cuts at: 387 2323 387
Size: 1936 5004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6940
NotI GC'GGCC_GC
Cuts at: 4387 4387
Size: 6940
NspI r_CATG'y
Cuts at: 1132 1229 2507 2632 4298 5843 1132
Size: 97 1278 125 1666 1545 2229
Fragments arranged by size:
2229 1666 1545 1278 125 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6940
PciI A'CATG_T
Cuts at: 5839 5839
Size: 6940
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6940
PfoI T'CCnGG_A
Cuts at: 3316 3316
Size: 6940
PmeI GTTT'AAAC
Cuts at: 4244 4244
Size: 6940
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5009 5041 6522 6554 5009
Size: 32 1481 32 5395
Fragments arranged by size:
5395 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3862 3862
Size: 6940
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6940
PsiI TTA'TAA
Cuts at: 4510 4510
Size: 6940
PstI C_TGCA'G
Cuts at: 830 2022 3870 3984 4292 830
Size: 1192 1848 114 308 3478
Fragments arranged by size:
3478 1848 1192 308 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6940
SalI G'TCGA_C
Cuts at: 1936 4282 1936
Size: 2346 4594
SapI GCTCTTCn'nnn_
Cuts at: 2385 2385
Size: 6940
SbfI CC_TGCA'GG
Cuts at: 4292 4292
Size: 6940
ScaI AGT'ACT
Cuts at: 4054 5188 6900 4054
Size: 1134 1712 4094
Fragments arranged by size:
4094 1712 1134
SfcI C'TryA_G
Cuts at: 826 1045 2018 2562 3786 3866 3980 4288 5423
Size: 219 973 544 1224 80 114 308 1135
Cuts at: 5423 6104 6295 826
Size: 681 191 1471
Fragments arranged by size:
1471 1224 1135 973 681 544 308 219
191 114 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6940
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6940
SmaI CCC'GGG
Cuts at: 1872 4267 1872
Size: 2395 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5044 5945 6207 820
Size: 197 926 3101 901 262 1553
Fragments arranged by size:
3101 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4731 493
Size: 4238 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6940
SphI G_CATG'C
Cuts at: 1132 4298 1132
Size: 3166 3774
SspI AAT'ATT
Cuts at: 5 52 3405 4864 5
Size: 47 3353 1459 2081
Fragments arranged by size:
3353 2081 1459 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6940
StyI C'CwwG_G
Cuts at: 513 1065 2004 2214 2373 3265 4379 513
Size: 552 939 210 159 892 1114 3074
Fragments arranged by size:
3074 1114 939 892 552 210 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4967 4984 5137 5322 5767 325
Size: 1255 3387 17 153 185 445 1498
Fragments arranged by size:
3387 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2273 3416 4052
Size: 274 80 33 51 1443 296 1143 636
Cuts at: 4052 4234 5186 6898 96
Size: 182 952 1712 138
Fragments arranged by size:
1712 1443 1143 952 636 296 274 182
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2071 2103 2940 2972 3650 3682 6900 6932 2071
Size: 32 837 32 678 32 3218 32 2079
Fragments arranged by size:
3218 2079 837 678 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6940
XbaI T'CTAG_A
Cuts at: 2242 4276 2242
Size: 2034 4906
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6940
XmaI C'CCGG_G
Cuts at: 1870 4265 1870
Size: 2395 4545
XmnI GAAnn'nnTTC
Cuts at: 5069 5069
Size: 6940
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4748 276
Size: 53 83 186 936 3214 2468
Fragments arranged by size:
3214 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI