Restriction map for
pFN21AE3288
(Circular) (Six-base) MAPSORT of: pFN21AE3288.seq Check: 34 from: 1 to: 6925
pFN21AE3288 6925 bp
With 182 enzymes: SgfI *
March 28, 2012 12:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2734 2734
Size: 6925
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2716 4735 278
Size: 53 83 186 936 1180 2019 2468
Fragments arranged by size:
2468 2019 1180 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3326 4268 1937
Size: 1389 942 4594
Fragments arranged by size:
4594 1389 942
Acc65I G'GTAC_C
Cuts at: 4246 4246
Size: 6925
AclI AA'CG_TT
Cuts at: 1563 5052 5425 1563
Size: 3489 373 3063
Fragments arranged by size:
3489 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2748 2888 3414 3820 4988 6372 6640 1632
Size: 1116 140 526 406 1168 1384 268 1917
Fragments arranged by size:
1917 1384 1168 1116 526 406 268 140
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6728
AflIII A'CryG_T
Cuts at: 1203 2506 2788 3875 5824 6801 1203
Size: 1303 282 1087 1949 977 1327
Fragments arranged by size:
1949 1327 1303 1087 977 282
AgeI A'CCGG_T
Cuts at: 5756 5756
Size: 6925
AhdI GACnn_n'nnGTC
Cuts at: 5654 5654
Size: 6925
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6925
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2138 1184
Size: 954 5971
AlwNI CAG_nnn'CTG
Cuts at: 2164 2950 4668 6240 2164
Size: 786 1718 1572 2849
Fragments arranged by size:
2849 1718 1572 786
ApaLI G'TGCA_C
Cuts at: 2113 4982 6138 2113
Size: 2869 1156 2900
Fragments arranged by size:
2900 2869 1156
ApoI r'AATT_y
Cuts at: 1493 2560 2829 3167 3698 4234 4460 4612 6721
Size: 1067 269 338 531 536 226 152 2109
Cuts at: 6721 1493
Size: 1697
Fragments arranged by size:
2109 1697 1067 536 531 338 269 226
152
AseI AT'TA_AT
Cuts at: 160 1032 2489 5479 160
Size: 872 1457 2990 1606
Fragments arranged by size:
2990 1606 1457 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6925
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 2219 4119 4250 1870
Size: 73 134 142 1900 131 4545
Fragments arranged by size:
4545 1900 142 134 131 73
BamHI G'GATC_C
Cuts at: 2263 4255 4622 2263
Size: 1992 367 4566
Fragments arranged by size:
4566 1992 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2082 2183 2241 4246 5701
Size: 325 217 627 295 101 58 2005 1455
Cuts at: 5701 618
Size: 1842
Fragments arranged by size:
2005 1842 1455 627 325 295 217 101
58
BanII G_rGCy'C
Cuts at: 1391 2587 4244 1391
Size: 1196 1657 4072
Fragments arranged by size:
4072 1657 1196
BbeI G_GCGC'C
Cuts at: 2086 2187 2245 2086
Size: 101 58 6766
Fragments arranged by size:
6766 101 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2044 928
Size: 971 145 5809
Fragments arranged by size:
5809 971 145
BbvCI CC'TCA_GC
Cuts at: 2688 2688
Size: 6925
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4100 4134 5116 5150 1903
Size: 34 2163 34 982 34 3678
Fragments arranged by size:
3678 2163 982 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3784 4817 6027 3784
Size: 1033 1210 4682
Fragments arranged by size:
4682 1210 1033
BclI T'GATC_A
Cuts at: 1548 2941 1548
Size: 1393 5532
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5536 136
Size: 107 122 71 631 605 337 3527 1525
Fragments arranged by size:
3527 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2737 3110 6920 2737
Size: 373 3810 2742
Fragments arranged by size:
3810 2742 373
BlpI GC'TnA_GC
Cuts at: 4342 4342
Size: 6925
Bme1580I G_kGCm'C
Cuts at: 1790 2110 2117 3082 4023 4986 6142 1790
Size: 320 7 965 941 963 1156 2573
Fragments arranged by size:
2573 1156 965 963 941 320 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6925
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2260 4656 5614
Size: 416 53 83 399 24 843 2396 958
Cuts at: 5614 442
Size: 1753
Fragments arranged by size:
2396 1753 958 843 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6925
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3370 3370
Size: 6925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 5585 1239
Size: 869 3477 2579
Fragments arranged by size:
3477 2579 869
Bpu10I CC'TnA_GC
Cuts at: 2688 2688
Size: 6925
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3029 3352 5050 5915 6213 3029
Size: 323 1698 865 298 3741
Fragments arranged by size:
3741 1698 865 323 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5588 882
Size: 617 4089 2219
Fragments arranged by size:
4089 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4716 493
Size: 711 3512 2702
Fragments arranged by size:
3512 2702 711
BsaBI GATnn'nnATC
Cuts at: 6919 6919
Size: 6925
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2083 2142 2169 2184
Size: 53 83 186 936 550 59 27 15
Cuts at: 2184 2242 2713 4732 5114 5749 275
Size: 58 471 2019 382 635 1451
Fragments arranged by size:
2019 1451 936 635 550 471 382 186
83 59 58 53 27 15
BsaWI w'CCGG_w
Cuts at: 3042 5358 5756 6030 6177 6588 3042
Size: 2316 398 274 147 411 3379
Fragments arranged by size:
3379 2316 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3227 3257 1178
Size: 30 165 30 1824 30 4846
Fragments arranged by size:
4846 1824 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2111 2327 1186
Size: 856 69 216 5784
Fragments arranged by size:
5784 856 216 69
BseYI C'CCAG_C
Cuts at: 3644 6128 6624 3644
Size: 2484 496 3945
Fragments arranged by size:
3945 2484 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3425 4094 1163
Size: 573 1689 669 3994
Fragments arranged by size:
3994 1689 669 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2135 2139 2176 2380 4375 5136
Size: 275 186 134 4 37 204 1995 761
Cuts at: 5136 6164 1540
Size: 1028 2301
Fragments arranged by size:
2301 1995 1028 761 275 204 186 134
37 4
BsiHKAI G_wGCw'C
Cuts at: 2117 4986 5071 6142 2117
Size: 2869 85 1071 2900
Fragments arranged by size:
2900 2869 1071 85
BsmI GAATG_Cn'
Cuts at: 4436 4529 4436
Size: 93 6832
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2117 2587 3082 4023 4244 4986
Size: 399 320 7 470 495 941 221 742
Cuts at: 4986 5071 6142 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 941 742 495 470 399 320
221 85 7
BspEI T'CCGG_A
Cuts at: 6588 6588
Size: 6925
BspHI T'CATG_A
Cuts at: 3678 4814 6544 3678
Size: 1136 1730 4059
Fragments arranged by size:
4059 1730 1136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2734 4280 844
Size: 1890 1546 3489
Fragments arranged by size:
3489 1890 1546
BsrBI CCG'CTC
Cuts at: 1394 1823 2076 3216 4812 1394
Size: 429 253 1140 1596 3507
Fragments arranged by size:
3507 1596 1140 429 253
BsrDI GCAATG_nn'
Cuts at: 65 1248 2568 4225 5420 5594 65
Size: 1183 1320 1657 1195 174 1396
Fragments arranged by size:
1657 1396 1320 1195 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2134 5569 5756 1700
Size: 110 324 3435 187 2869
Fragments arranged by size:
3435 2869 324 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6925
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6925
BssSI C'ACGA_G
Cuts at: 2356 4985 5997 2356
Size: 2629 1012 3284
Fragments arranged by size:
3284 2629 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6925
BstBI TT'CG_AA
Cuts at: 5729 5729
Size: 6925
BstEII G'GTnAC_C
Cuts at: 1184 2746 1184
Size: 1562 5363
BstXI CCAn_nnnn'nTGG
Cuts at: 3955 3955
Size: 6925
BstYI r'GATC_y
Cuts at: 1972 2263 2737 2965 3110 3337 4157 4255 4622
Size: 291 474 228 145 227 820 98 367
Cuts at: 4622 5007 5024 6476 6562 6574 6920 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 820 474 385 367 346 291
228 227 145 98 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2230 513
Size: 552 939 226 5208
Fragments arranged by size:
5208 939 552 226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6186
Fragments arranged by size:
6186 683 56
BtsI GCAGTG_nn'
Cuts at: 3663 3984 4441 5235 5255 3663
Size: 321 457 794 20 5333
Fragments arranged by size:
5333 794 457 321 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6925
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6890
DraI TTT'AAA
Cuts at: 4229 4229
Size: 6925
DraIII CAC_nnn'GTG
Cuts at: 2358 3403 2358
Size: 1045 5880
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5932 809
Size: 507 4616 1802
Fragments arranged by size:
4616 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2132 2136 2349
Size: 54 1425 50 275 18 302 4 213
Cuts at: 2349 2377 2565 4038 4372 5261 6890 8
Size: 28 188 1473 334 889 1629 43
Fragments arranged by size:
1629 1473 1425 889 334 302 275 213
188 54 50 43 28 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2132 2136 2377 4372 1537
Size: 275 320 4 241 1995 4090
Fragments arranged by size:
4090 1995 320 275 241 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2433 2573 2616 3223 3456 4855 1353
Size: 1080 140 43 607 233 1399 3423
Fragments arranged by size:
3423 1399 1080 607 233 140 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 5512 5886 6032 387
Size: 1064 138 438 3485 374 146 1280
Fragments arranged by size:
3485 1280 1064 438 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2748 2888 3414 3820 4988 5585
Size: 393 476 640 140 526 406 1168 597
Cuts at: 5585 6372 6640 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1168 787 640 597 526 476 406
393 268 140
EcoO109I rG'GnC_Cy
Cuts at: 2223 2255 2223
Size: 32 6893
EcoRI G'AATT_C
Cuts at: 4234 4612 4234
Size: 378 6547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3850 3850
Size: 6925
FseI GG_CCGG'CC
Cuts at: 2138 2138
Size: 6925
FspI TGC'GCA
Cuts at: 5431 5431
Size: 6925
HaeII r_GCGC'y
Cuts at: 1678 2086 2187 2245 2348 3720 6072 1678
Size: 408 101 58 103 1372 2352 2531
Fragments arranged by size:
2531 2352 1372 408 103 101 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3194 3226 3227 3259 4142
Size: 32 135 32 1622 32 1 32 883
Cuts at: 4142 4174 5566 5598 5640 5672 1373
Size: 32 1392 32 42 32 2626
Fragments arranged by size:
2626 1622 1392 883 135 42 32 32
32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3289 4269 4515 669
Size: 988 281 1351 980 246 3079
Fragments arranged by size:
3079 1351 988 980 281 246
HindIII A'AGCT_T
Cuts at: 748 3886 748
Size: 3138 3787
HpaI GTT'AAC
Cuts at: 4515 4515
Size: 6925
KasI G'GCGC_C
Cuts at: 2082 2183 2241 2082
Size: 101 58 6766
Fragments arranged by size:
6766 101 58
KpnI G_GTAC'C
Cuts at: 4250 4250
Size: 6925
MfeI C'AATT_G
Cuts at: 4524 4524
Size: 6925
MluI A'CGCG_T
Cuts at: 2788 6801 2788
Size: 4013 2912
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2598 6039 6223 1305
Size: 1293 3441 184 2007
Fragments arranged by size:
3441 2007 1293 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2351 2567 4040 10
Size: 54 1425 343 519 216 1473 2895
Fragments arranged by size:
2895 1473 1425 519 343 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 2573 4883 5242 5401 518
Size: 648 1386 21 2310 359 159 2042
Fragments arranged by size:
2310 2042 1386 648 359 159 21
MspA1I CmG'CkG
Cuts at: 1606 2161 2232 3318 3797 4341 5018 6166 6411
Size: 555 71 1086 479 544 677 1148 245
Cuts at: 6411 1606
Size: 2120
Fragments arranged by size:
2120 1148 1086 677 555 544 479 245
71
NaeI GCC'GGC
Cuts at: 2136 2136
Size: 6925
NarI GG'CG_CC
Cuts at: 2083 2184 2242 2083
Size: 101 58 6766
Fragments arranged by size:
6766 101 58
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5434
Fragments arranged by size:
5434 939 552
NdeI CA'TA_TG
Cuts at: 387 2500 387
Size: 2113 4812
NgoMIV G'CCGG_C
Cuts at: 2134 2134
Size: 6925
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6925
NotI GC'GGCC_GC
Cuts at: 4372 4372
Size: 6925
NspI r_CATG'y
Cuts at: 1132 1229 2510 3879 4283 5828 1132
Size: 97 1281 1369 404 1545 2229
Fragments arranged by size:
2229 1545 1369 1281 404 97
PasI CC'CwG_GG
Cuts at: 1348 3724 4016 1348
Size: 2376 292 4257
Fragments arranged by size:
4257 2376 292
PciI A'CATG_T
Cuts at: 2506 3875 5824 2506
Size: 1369 1949 3607
Fragments arranged by size:
3607 1949 1369
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2164 1108
Size: 1056 5869
PfoI T'CCnGG_A
Cuts at: 2196 2196
Size: 6925
PmeI GTTT'AAAC
Cuts at: 4229 4229
Size: 6925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4994 5026 6507 6539 4994
Size: 32 1481 32 5380
Fragments arranged by size:
5380 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2223 2255 2223
Size: 32 6893
PshAI GACnn'nnGTC
Cuts at: 1613 2253 1613
Size: 640 6285
PsiI TTA'TAA
Cuts at: 2513 4495 2513
Size: 1982 4943
PstI C_TGCA'G
Cuts at: 830 2653 2745 3270 3658 3783 4277 830
Size: 1823 92 525 388 125 494 3478
Fragments arranged by size:
3478 1823 525 494 388 125 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6925
PvuII CAG'CTG
Cuts at: 3797 3797
Size: 6925
SacII CC_GC'GG
Cuts at: 2233 2233
Size: 6925
SalI G'TCGA_C
Cuts at: 1936 4267 1936
Size: 2331 4594
SanDI GG'GwC_CC
Cuts at: 2223 2223
Size: 6925
SapI GCTCTTCn'nnn_
Cuts at: 2433 3223 2433
Size: 790 6135
SbfI CC_TGCA'GG
Cuts at: 4277 4277
Size: 6925
ScaI AGT'ACT
Cuts at: 5173 6885 5173
Size: 1712 5213
SexAI A'CCwGG_T
Cuts at: 3585 3585
Size: 6925
SfcI C'TryA_G
Cuts at: 826 1045 2649 2741 3266 3654 3779 3993 4273
Size: 219 1604 92 525 388 125 214 280
Cuts at: 4273 5408 6089 6280 826
Size: 1135 681 191 1471
Fragments arranged by size:
1604 1471 1135 681 525 388 280 219
214 191 125 92
SfoI GGC'GCC
Cuts at: 2084 2185 2243 2084
Size: 101 58 6766
Fragments arranged by size:
6766 101 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6925
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6925
SmaI CCC'GGG
Cuts at: 1872 4252 1872
Size: 2380 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3008 3331 4119 5029 5930 6192
Size: 197 926 1065 323 788 910 901 262
Cuts at: 6192 820
Size: 1553
Fragments arranged by size:
1553 1065 926 910 901 788 323 262
197
SnaBI TAC'GTA
Cuts at: 493 4716 493
Size: 4223 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6925
SphI G_CATG'C
Cuts at: 1132 4283 1132
Size: 3151 3774
SspI AAT'ATT
Cuts at: 5 52 4849 5
Size: 47 4797 2081
Fragments arranged by size:
4797 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6925
StyI C'CwwG_G
Cuts at: 513 1065 2004 3511 4364 513
Size: 552 939 1507 853 3074
Fragments arranged by size:
3074 1507 939 853 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4952 4969 5122 5307 5752 325
Size: 1255 3372 17 153 185 445 1498
Fragments arranged by size:
3372 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3637 5171 6883
Size: 274 80 33 51 1443 1660 1534 1712
Cuts at: 6883 96
Size: 138
Fragments arranged by size:
1712 1660 1534 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3265 3297 3427 3459 6885 6917 3265
Size: 32 130 32 3426 32 3273
Fragments arranged by size:
3426 3273 130 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6925
XbaI T'CTAG_A
Cuts at: 3152 4261 3152
Size: 1109 5816
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2405 2405
Size: 6925
XhoI C'TCGA_G
Cuts at: 1943 4119 1943
Size: 2176 4749
XmaI C'CCGG_G
Cuts at: 1870 4250 1870
Size: 2380 4545
XmnI GAAnn'nnTTC
Cuts at: 5054 5054
Size: 6925
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2714 4733 276
Size: 53 83 186 936 1180 2019 2468
Fragments arranged by size:
2468 2019 1180 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FspAI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SfiI SrfI SwaI