Restriction map for
pFN21AE3287
(Circular) (Six-base) MAPSORT of: pFN21AE3287.seq Check: 8729 from: 1 to: 6925
pFN21AE3287 6925 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2025 2025
Size: 6925
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4735 278
Size: 53 83 186 936 3199 2468
Fragments arranged by size:
3199 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4268 1937
Size: 2331 4594
Acc65I G'GTAC_C
Cuts at: 2263 4246 2263
Size: 1983 4942
AclI AA'CG_TT
Cuts at: 1563 5052 5425 1563
Size: 3489 373 3063
Fragments arranged by size:
3489 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2939 3099 3389 4988 6372 6640 1632
Size: 1307 160 290 1599 1384 268 1917
Fragments arranged by size:
1917 1599 1384 1307 290 268 160
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6728
AflIII A'CryG_T
Cuts at: 1203 4189 5824 6801 1203
Size: 2986 1635 977 1327
Fragments arranged by size:
2986 1635 1327 977
AgeI A'CCGG_T
Cuts at: 5756 5756
Size: 6925
AhdI GACnn_n'nnGTC
Cuts at: 5654 5654
Size: 6925
AleI CACnn'nnGTG
Cuts at: 1166 3902 1166
Size: 2736 4189
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3321 1184
Size: 2137 4788
AlwNI CAG_nnn'CTG
Cuts at: 2581 4668 6240 2581
Size: 2087 1572 3266
Fragments arranged by size:
3266 2087 1572
ApaLI G'TGCA_C
Cuts at: 2125 4982 6138 2125
Size: 2857 1156 2912
Fragments arranged by size:
2912 2857 1156
ApoI r'AATT_y
Cuts at: 1493 2275 2304 2894 2963 3743 4234 4460 4612
Size: 782 29 590 69 780 491 226 152
Cuts at: 4612 6721 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 782 780 590 491 226 152
69 29
AseI AT'TA_AT
Cuts at: 160 1032 5479 160
Size: 872 4447 1606
Fragments arranged by size:
4447 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6925
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 2110 2155 4250 1870
Size: 73 121 46 45 2095 4545
Fragments arranged by size:
4545 2095 121 73 46 45
BamHI G'GATC_C
Cuts at: 4255 4622 4255
Size: 367 6558
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2263 3896 4246 5701
Size: 325 217 627 372 104 1633 350 1455
Cuts at: 5701 618
Size: 1842
Fragments arranged by size:
1842 1633 1455 627 372 350 325 217
104
BanII G_rGCy'C
Cuts at: 1391 2102 3638 4244 1391
Size: 711 1536 606 4072
Fragments arranged by size:
4072 1536 711 606
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3534 928
Size: 971 1635 4319
Fragments arranged by size:
4319 1635 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5116 5150 1903
Size: 34 3179 34 3678
Fragments arranged by size:
3678 3179 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4817 6027 4817
Size: 1210 5715
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6925
BfrBI ATG'CAT
Cuts at: 2353 2353
Size: 6925
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5536 136
Size: 107 122 71 631 605 3864 1525
Fragments arranged by size:
3864 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2403 3286 6920 2403
Size: 883 3634 2408
Fragments arranged by size:
3634 2408 883
BlpI GC'TnA_GC
Cuts at: 4342 4342
Size: 6925
Bme1580I G_kGCm'C
Cuts at: 1790 2129 2162 2253 2483 3154 4986 6142 1790
Size: 339 33 91 230 671 1832 1156 2573
Fragments arranged by size:
2573 1832 1156 671 339 230 91 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6925
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4656 5614 442
Size: 416 53 83 399 24 3239 958 1753
Fragments arranged by size:
3239 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 5585 1239
Size: 863 3483 2579
Fragments arranged by size:
3483 2579 863
Bpu10I CC'TnA_GC
Cuts at: 2193 2193
Size: 6925
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2830 3614 5050 5915 6213 2830
Size: 784 1436 865 298 3542
Fragments arranged by size:
3542 1436 865 784 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2290 5588 882
Size: 617 791 3298 2219
Fragments arranged by size:
3298 2219 791 617
BsaAI yAC'GTr
Cuts at: 493 1204 4716 493
Size: 711 3512 2702
Fragments arranged by size:
3512 2702 711
BsaBI GATnn'nnATC
Cuts at: 2352 6919 2352
Size: 4567 2358
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4732 5114 5749 275
Size: 53 83 186 936 3199 382 635 1451
Fragments arranged by size:
3199 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5358 5756 6030 6177 6588 5358
Size: 398 274 147 411 5695
Fragments arranged by size:
5695 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2189 2219 2429 2459 1178
Size: 30 165 30 786 30 210 30 5644
Fragments arranged by size:
5644 786 210 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 3546 4214 1186
Size: 841 1519 668 3897
Fragments arranged by size:
3897 1519 841 668
BseYI C'CCAG_C
Cuts at: 6128 6624 6128
Size: 496 6429
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6352
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4375 5136 6164 1540
Size: 275 186 2374 761 1028 2301
Fragments arranged by size:
2374 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2102 2129 2223 4986 5071 6142 2102
Size: 27 94 2763 85 1071 2885
Fragments arranged by size:
2885 2763 1071 94 85 27
BsmI GAATG_Cn'
Cuts at: 3255 4436 4529 3255
Size: 1181 93 5651
Fragments arranged by size:
5651 1181 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2129 2162 2223 2253 2483 3154
Size: 399 312 27 33 61 30 230 671
Cuts at: 3154 3638 4244 4986 5071 6142 1391
Size: 484 606 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 671 606 484 399 312
230 85 61 33 30 27
BspEI T'CCGG_A
Cuts at: 6588 6588
Size: 6925
BspHI T'CATG_A
Cuts at: 3574 4814 6544 3574
Size: 1240 1730 3955
Fragments arranged by size:
3955 1730 1240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2025 3260 3271 4280 844
Size: 1181 1235 11 1009 3489
Fragments arranged by size:
3489 1235 1181 1009 11
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 4812 1394
Size: 429 212 2777 3507
Fragments arranged by size:
3507 2777 429 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 5420 5594 65
Size: 1183 4172 174 1396
Fragments arranged by size:
4172 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5569 5756 1700
Size: 110 3759 187 2869
Fragments arranged by size:
3759 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2049 3445 96
Size: 1953 1396 3576
Fragments arranged by size:
3576 1953 1396
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6925
BssSI C'ACGA_G
Cuts at: 2039 2293 2874 4985 5997 2039
Size: 254 581 2111 1012 2967
Fragments arranged by size:
2967 2111 1012 581 254
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6925
BstBI TT'CG_AA
Cuts at: 5729 5729
Size: 6925
BstEII G'GTnAC_C
Cuts at: 1184 2858 1184
Size: 1674 5251
BstXI CCAn_nnnn'nTGG
Cuts at: 2260 2260
Size: 6925
BstYI r'GATC_y
Cuts at: 1972 2403 3286 4255 4622 5007 5024 6476 6562
Size: 431 883 969 367 385 17 1452 86
Cuts at: 6562 6574 6920 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 969 883 431 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5434
Fragments arranged by size:
5434 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3615 487
Size: 683 56 2389 3797
Fragments arranged by size:
3797 2389 683 56
BtsI GCAGTG_nn'
Cuts at: 4441 5235 5255 4441
Size: 794 20 6111
Fragments arranged by size:
6111 794 20
ClaI AT'CG_AT
Cuts at: 1554 3318 1554
Size: 1764 5161
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6890
DraI TTT'AAA
Cuts at: 2743 4229 2743
Size: 1486 5439
DraIII CAC_nnn'GTG
Cuts at: 2478 3454 2478
Size: 976 5949
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5932 809
Size: 507 4616 1802
Fragments arranged by size:
4616 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4372 5261 6890
Size: 54 1425 50 275 18 2542 889 1629
Cuts at: 6890 8
Size: 43
Fragments arranged by size:
2542 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4372 1537
Size: 275 2560 4090
Fragments arranged by size:
4090 2560 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4855 1353
Size: 3502 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3612 5512 5886 6032 387
Size: 1064 138 2023 1900 374 146 1280
Fragments arranged by size:
2023 1900 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2100 2100
Size: 6925
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2939 3099 3389 4988 5585 6372
Size: 393 470 837 160 290 1599 597 787
Cuts at: 6372 6640 1239
Size: 268 1524
Fragments arranged by size:
1599 1524 837 787 597 470 393 290
268 160
EcoNI CCTnn'n_nnAGG
Cuts at: 3005 3503 3005
Size: 498 6427
EcoO109I rG'GnC_Cy
Cuts at: 3039 4041 3039
Size: 1002 5923
EcoRI G'AATT_C
Cuts at: 3743 4234 4612 3743
Size: 491 378 6056
Fragments arranged by size:
6056 491 378
EcoRV GAT'ATC
Cuts at: 3192 3192
Size: 6925
FspI TGC'GCA
Cuts at: 5431 5431
Size: 6925
HaeII r_GCGC'y
Cuts at: 1678 6072 1678
Size: 4394 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2828 2860 5566 5598 5640
Size: 32 135 32 1256 32 2706 32 42
Cuts at: 5640 5672 1373
Size: 32 2626
Fragments arranged by size:
2706 2626 1256 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2236 4269 4515 669
Size: 988 281 298 2033 246 3079
Fragments arranged by size:
3079 2033 988 298 281 246
HindIII A'AGCT_T
Cuts at: 748 3081 748
Size: 2333 4592
HpaI GTT'AAC
Cuts at: 4515 4515
Size: 6925
KpnI G_GTAC'C
Cuts at: 2267 4250 2267
Size: 1983 4942
MfeI C'AATT_G
Cuts at: 4524 4524
Size: 6925
MluI A'CGCG_T
Cuts at: 6801 6801
Size: 6925
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6039 6223 1305
Size: 4734 184 2007
Fragments arranged by size:
4734 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5103
Fragments arranged by size:
5103 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2358 3303 3441 3695 3902 4883 5242
Size: 648 1192 945 138 254 207 981 359
Cuts at: 5242 5401 518
Size: 159 2042
Fragments arranged by size:
2042 1192 981 945 648 359 254 207
159 138
MspA1I CmG'CkG
Cuts at: 1606 2423 2658 3059 4341 5018 6166 6411 1606
Size: 817 235 401 1282 677 1148 245 2120
Fragments arranged by size:
2120 1282 1148 817 677 401 245 235
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5434
Fragments arranged by size:
5434 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6925
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6925
NotI GC'GGCC_GC
Cuts at: 4372 4372
Size: 6925
NsiI A_TGCA'T
Cuts at: 2355 2355
Size: 6925
NspI r_CATG'y
Cuts at: 1132 1229 3302 3335 3801 4283 5828 1132
Size: 97 2073 33 466 482 1545 2229
Fragments arranged by size:
2229 2073 1545 482 466 97 33
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6925
PciI A'CATG_T
Cuts at: 5824 5824
Size: 6925
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6925
PfoI T'CCnGG_A
Cuts at: 2580 2805 2580
Size: 225 6700
PmeI GTTT'AAAC
Cuts at: 4229 4229
Size: 6925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4994 5026 6507 6539 4994
Size: 32 1481 32 5380
Fragments arranged by size:
5380 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3039 3039
Size: 6925
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6925
PsiI TTA'TAA
Cuts at: 3005 4495 3005
Size: 1490 5435
PstI C_TGCA'G
Cuts at: 830 4277 830
Size: 3447 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6925
PvuII CAG'CTG
Cuts at: 2423 2658 3059 2423
Size: 235 401 6289
Fragments arranged by size:
6289 401 235
SacI G_AGCT'C
Cuts at: 2102 2102
Size: 6925
SalI G'TCGA_C
Cuts at: 1936 4267 1936
Size: 2331 4594
SanDI GG'GwC_CC
Cuts at: 3039 3039
Size: 6925
SbfI CC_TGCA'GG
Cuts at: 4277 4277
Size: 6925
ScaI AGT'ACT
Cuts at: 5173 6885 5173
Size: 1712 5213
SfcI C'TryA_G
Cuts at: 826 1045 3182 4273 5408 6089 6280 826
Size: 219 2137 1091 1135 681 191 1471
Fragments arranged by size:
2137 1471 1135 1091 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6925
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6925
SmaI CCC'GGG
Cuts at: 1872 2157 4252 1872
Size: 285 2095 4545
Fragments arranged by size:
4545 2095 285
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2845 3629 5029 5930 6192 820
Size: 197 926 902 784 1400 901 262 1553
Fragments arranged by size:
1553 1400 926 902 901 784 262 197
SnaBI TAC'GTA
Cuts at: 493 4716 493
Size: 4223 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6925
SphI G_CATG'C
Cuts at: 1132 3302 4283 1132
Size: 2170 981 3774
Fragments arranged by size:
3774 2170 981
SspI AAT'ATT
Cuts at: 5 52 4849 5
Size: 47 4797 2081
Fragments arranged by size:
4797 2081 47
StuI AGG'CCT
Cuts at: 1517 3653 1517
Size: 2136 4789
StyI C'CwwG_G
Cuts at: 513 1065 2004 2685 3034 3270 4364 513
Size: 552 939 681 349 236 1094 3074
Fragments arranged by size:
3074 1094 939 681 552 349 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2153 3963 4952 4969 5122 5307 5752
Size: 1255 573 1810 989 17 153 185 445
Cuts at: 5752 325
Size: 1498
Fragments arranged by size:
1810 1498 1255 989 573 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2049 3445 5171
Size: 274 80 33 51 1443 72 1396 1726
Cuts at: 5171 6883 96
Size: 1712 138
Fragments arranged by size:
1726 1712 1443 1396 274 138 80 72
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4118 4150 6885 6917 4118
Size: 32 2735 32 4126
Fragments arranged by size:
4126 2735 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6925
XbaI T'CTAG_A
Cuts at: 4261 4261
Size: 6925
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3809 3809
Size: 6925
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6925
XmaI C'CCGG_G
Cuts at: 1870 2155 4250 1870
Size: 285 2095 4545
Fragments arranged by size:
4545 2095 285
XmnI GAAnn'nnTTC
Cuts at: 3541 5054 3541
Size: 1513 5412
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4733 276
Size: 53 83 186 936 3199 2468
Fragments arranged by size:
3199 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I Bsu36I FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI
SrfI SwaI