Restriction map for
pFN21AE3268
(Circular) (Six-base) MAPSORT of: pFN21AE3268.seq Check: 9923 from: 1 to: 6703
pFN21AE3268 6703 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4513 278
Size: 53 83 186 936 2977 2468
Fragments arranged by size:
2977 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3439 4046 1937
Size: 1502 607 4594
Fragments arranged by size:
4594 1502 607
Acc65I G'GTAC_C
Cuts at: 4024 4024
Size: 6703
AclI AA'CG_TT
Cuts at: 1563 3628 4830 5203 1563
Size: 2065 1202 373 3063
Fragments arranged by size:
3063 2065 1202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2955 3460 4766 6150 6418 1632
Size: 1323 505 1306 1384 268 1917
Fragments arranged by size:
1917 1384 1323 1306 505 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6506
AflIII A'CryG_T
Cuts at: 1203 5602 6579 1203
Size: 4399 977 1327
Fragments arranged by size:
4399 1327 977
AgeI A'CCGG_T
Cuts at: 5534 5534
Size: 6703
AhdI GACnn_n'nnGTC
Cuts at: 3452 5432 3452
Size: 1980 4723
AleI CACnn'nnGTG
Cuts at: 1166 2912 1166
Size: 1746 4957
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3368 1184
Size: 2184 4519
AlwNI CAG_nnn'CTG
Cuts at: 2090 2822 4446 6018 2090
Size: 732 1624 1572 2775
Fragments arranged by size:
2775 1624 1572 732
ApaLI G'TGCA_C
Cuts at: 4760 5916 4760
Size: 1156 5547
ApoI r'AATT_y
Cuts at: 1493 2485 2536 2686 2737 3194 4012 4238 4390
Size: 992 51 150 51 457 818 226 152
Cuts at: 4390 6499 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 992 818 457 226 152 150
51 51
AseI AT'TA_AT
Cuts at: 160 1032 5257 160
Size: 872 4225 1606
Fragments arranged by size:
4225 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6703
AvaI C'yCGr_G
Cuts at: 1870 1943 4028 1870
Size: 73 2085 4545
Fragments arranged by size:
4545 2085 73
BamHI G'GATC_C
Cuts at: 4033 4400 4033
Size: 367 6336
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2914 4024 5479 618
Size: 325 217 627 1127 1110 1455 1842
Fragments arranged by size:
1842 1455 1127 1110 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3162 4022 1391
Size: 1771 860 4072
Fragments arranged by size:
4072 1771 860
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2361 2994 928
Size: 971 462 633 4637
Fragments arranged by size:
4637 971 633 462
BbvCI CC'TCA_GC
Cuts at: 2586 2586
Size: 6703
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4894 4928 1903
Size: 34 2957 34 3678
Fragments arranged by size:
3678 2957 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3267 4595 5805 3267
Size: 1328 1210 4165
Fragments arranged by size:
4165 1328 1210
BclI T'GATC_A
Cuts at: 1548 2092 2140 3972 1548
Size: 544 48 1832 4279
Fragments arranged by size:
4279 1832 544 48
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5314 136
Size: 107 122 71 631 605 3642 1525
Fragments arranged by size:
3642 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6698 6698
Size: 6703
BlpI GC'TnA_GC
Cuts at: 2052 3698 4120 2052
Size: 1646 422 4635
Fragments arranged by size:
4635 1646 422
Bme1580I G_kGCm'C
Cuts at: 1790 2509 4764 5920 1790
Size: 719 2255 1156 2573
Fragments arranged by size:
2573 2255 1156 719
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6703
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4434 5392 442
Size: 416 53 83 399 24 3017 958 1753
Fragments arranged by size:
3017 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6703
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2988 5363 1239
Size: 1749 2375 2579
Fragments arranged by size:
2579 2375 1749
Bpu10I CC'TnA_GC
Cuts at: 2586 2586
Size: 6703
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4828 5693 5991 4828
Size: 865 298 5540
Fragments arranged by size:
5540 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3062 5366 882
Size: 617 1563 2304 2219
Fragments arranged by size:
2304 2219 1563 617
BsaAI yAC'GTr
Cuts at: 493 1204 3647 4494 493
Size: 711 2443 847 2702
Fragments arranged by size:
2702 2443 847 711
BsaBI GATnn'nnATC
Cuts at: 2091 2316 3560 6697 2091
Size: 225 1244 3137 2097
Fragments arranged by size:
3137 2097 1244 225
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 4510 4892 5527
Size: 53 83 186 936 504 2473 382 635
Cuts at: 5527 275
Size: 1451
Fragments arranged by size:
2473 1451 936 635 504 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3914 5136 5534 5808 5955 6366 3914
Size: 1222 398 274 147 411 4251
Fragments arranged by size:
4251 1222 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6478
Fragments arranged by size:
6478 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2513 2591 2636 3068 3749 3752 3764 1186
Size: 1327 78 45 432 681 3 12 4125
Fragments arranged by size:
4125 1327 681 432 78 45 12 3
BseYI C'CCAG_C
Cuts at: 5906 6402 5906
Size: 496 6207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2976 1163
Size: 573 1240 4890
Fragments arranged by size:
4890 1240 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2654 4153 4914 5942 1540
Size: 275 186 653 1499 761 1028 2301
Fragments arranged by size:
2301 1499 1028 761 653 275 186
BsiHKAI G_wGCw'C
Cuts at: 4764 4849 5920 4764
Size: 85 1071 5547
Fragments arranged by size:
5547 1071 85
BsmI GAATG_Cn'
Cuts at: 2107 2782 2878 3596 3795 4214 4307 2107
Size: 675 96 718 199 419 93 4503
Fragments arranged by size:
4503 718 675 419 199 96 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2509 3162 4022 4764 4849 5920 1391
Size: 399 719 653 860 742 85 1071 2174
Fragments arranged by size:
2174 1071 860 742 719 653 399 85
BspEI T'CCGG_A
Cuts at: 3914 6366 3914
Size: 2452 4251
BspHI T'CATG_A
Cuts at: 4592 6322 4592
Size: 1730 4973
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4058 844
Size: 3214 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4590 1394
Size: 429 2767 3507
Fragments arranged by size:
3507 2767 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3319 3370 5198 5372 65
Size: 1183 2071 51 1828 174 1396
Fragments arranged by size:
2071 1828 1396 1183 174 51
BsrFI r'CCGG_y
Cuts at: 1700 1810 2653 5347 5534 1700
Size: 110 843 2694 187 2869
Fragments arranged by size:
2869 2694 843 187 110
BsrGI T'GTAC_A
Cuts at: 96 3381 96
Size: 3285 3418
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6703
BssSI C'ACGA_G
Cuts at: 4763 5775 4763
Size: 1012 5691
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6703
BstBI TT'CG_AA
Cuts at: 5507 5507
Size: 6703
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6703
BstXI CCAn_nnnn'nTGG
Cuts at: 3712 3763 3712
Size: 51 6652
BstYI r'GATC_y
Cuts at: 1972 2020 2075 2513 4033 4400 4785 4802 6254
Size: 48 55 438 1520 367 385 17 1452
Cuts at: 6254 6340 6352 6698 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1520 1452 438 385 367 346 86
55 48 17 12
Bsu36I CC'TnA_GG
Cuts at: 3036 3036
Size: 6703
BtgI C'CryG_G
Cuts at: 513 1065 2004 2937 513
Size: 552 939 933 4279
Fragments arranged by size:
4279 939 933 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3993 487
Size: 683 56 2767 3197
Fragments arranged by size:
3197 2767 683 56
BtsI GCAGTG_nn'
Cuts at: 2839 3392 4219 5013 5033 2839
Size: 553 827 794 20 4509
Fragments arranged by size:
4509 827 794 553 20
ClaI AT'CG_AT
Cuts at: 1554 3724 1554
Size: 2170 4533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6668
DraI TTT'AAA
Cuts at: 3460 4007 3460
Size: 547 6156
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5710 809
Size: 507 4394 1802
Fragments arranged by size:
4394 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2651 4150 5039
Size: 54 1425 50 275 18 821 1499 889
Cuts at: 5039 6668 8
Size: 1629 43
Fragments arranged by size:
1629 1499 1425 889 821 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2651 4150 1537
Size: 275 839 1499 4090
Fragments arranged by size:
4090 1499 839 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2030 2718 2790 2974 3045 3978 4633 1353
Size: 677 688 72 184 71 933 655 3423
Fragments arranged by size:
3423 933 688 677 655 184 72 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5290 5664 5810 387
Size: 1064 138 435 3266 374 146 1280
Fragments arranged by size:
3266 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2955 2988 3460 4766 5363 6150 6418
Size: 393 1323 33 472 1306 597 787 268
Cuts at: 6418 1239
Size: 1524
Fragments arranged by size:
1524 1323 1306 787 597 472 393 268
33
EcoNI CCTnn'n_nnAGG
Cuts at: 3003 3003
Size: 6703
EcoRI G'AATT_C
Cuts at: 2536 2686 4012 4390 2536
Size: 150 1326 378 4849
Fragments arranged by size:
4849 1326 378 150
EcoRV GAT'ATC
Cuts at: 2965 2965
Size: 6703
FspI TGC'GCA
Cuts at: 5209 5209
Size: 6703
HaeII r_GCGC'y
Cuts at: 1678 3667 3705 5850 1678
Size: 1989 38 2145 2531
Fragments arranged by size:
2531 2145 1989 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2228 2249 2260
Size: 32 135 32 488 32 136 21 11
Cuts at: 2260 2281 2798 2830 2951 2983 3438 3470 3801
Size: 21 517 32 121 32 455 32 331
Cuts at: 3801 3827 3833 3859 5344 5376 5418 5450 1373
Size: 26 6 26 1485 32 42 32 2626
Fragments arranged by size:
2626 1485 517 488 455 331 136 135
121 42 32 32 32 32 32 32
32 32 26 26 21 21 11 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4047 4293 669
Size: 988 281 2109 246 3079
Fragments arranged by size:
3079 2109 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2271 748
Size: 1523 5180
HpaI GTT'AAC
Cuts at: 4293 4293
Size: 6703
KpnI G_GTAC'C
Cuts at: 4028 4028
Size: 6703
MfeI C'AATT_G
Cuts at: 4302 4302
Size: 6703
MluI A'CGCG_T
Cuts at: 6579 6579
Size: 6703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5817 6001 1305
Size: 4512 184 2007
Fragments arranged by size:
4512 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4881
Fragments arranged by size:
4881 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2226 2424 2787 2912 3605 4661 5020
Size: 648 1060 198 363 125 693 1056 359
Cuts at: 5020 5179 518
Size: 159 2042
Fragments arranged by size:
2042 1060 1056 693 648 363 359 198
159 125
MspA1I CmG'CkG
Cuts at: 1606 2713 3691 4119 4796 5944 6189 1606
Size: 1107 978 428 677 1148 245 2120
Fragments arranged by size:
2120 1148 1107 978 677 428 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2937 513
Size: 552 939 933 4279
Fragments arranged by size:
4279 939 933 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6703
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6703
NotI GC'GGCC_GC
Cuts at: 4150 4150
Size: 6703
NspI r_CATG'y
Cuts at: 1132 1229 3349 4061 5606 1132
Size: 97 2120 712 1545 2229
Fragments arranged by size:
2229 2120 1545 712 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6703
PciI A'CATG_T
Cuts at: 5602 5602
Size: 6703
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3575 1108
Size: 2467 4236
PfoI T'CCnGG_A
Cuts at: 3004 3004
Size: 6703
PmeI GTTT'AAAC
Cuts at: 4007 4007
Size: 6703
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4772 4804 6285 6317 4772
Size: 32 1481 32 5158
Fragments arranged by size:
5158 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2361 1613
Size: 748 5955
PsiI TTA'TAA
Cuts at: 3422 4273 3422
Size: 851 5852
PstI C_TGCA'G
Cuts at: 830 2535 3399 4055 830
Size: 1705 864 656 3478
Fragments arranged by size:
3478 1705 864 656
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6703
PvuII CAG'CTG
Cuts at: 2713 2713
Size: 6703
SalI G'TCGA_C
Cuts at: 1936 4045 1936
Size: 2109 4594
SbfI CC_TGCA'GG
Cuts at: 4055 4055
Size: 6703
ScaI AGT'ACT
Cuts at: 4951 6663 4951
Size: 1712 4991
SexAI A'CCwGG_T
Cuts at: 3233 3233
Size: 6703
SfcI C'TryA_G
Cuts at: 826 1045 2531 3395 3440 4051 5186 5867 6058
Size: 219 1486 864 45 611 1135 681 191
Cuts at: 6058 826
Size: 1471
Fragments arranged by size:
1486 1471 1135 864 681 611 219 191
45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6703
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6703
SmaI CCC'GGG
Cuts at: 1872 4030 1872
Size: 2158 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4807 5708 5970 820
Size: 197 926 2864 901 262 1553
Fragments arranged by size:
2864 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3647 4494 493
Size: 3154 847 2702
Fragments arranged by size:
3154 2702 847
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6703
SphI G_CATG'C
Cuts at: 1132 4061 1132
Size: 2929 3774
SspI AAT'ATT
Cuts at: 5 52 4627 5
Size: 47 4575 2081
Fragments arranged by size:
4575 2081 47
StuI AGG'CCT
Cuts at: 1517 2584 2762 1517
Size: 1067 178 5458
Fragments arranged by size:
5458 1067 178
StyI C'CwwG_G
Cuts at: 513 1065 2004 2937 4142 513
Size: 552 939 933 1205 3074
Fragments arranged by size:
3074 1205 939 933 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4730 4747 4900 5085 5530 325
Size: 1255 3150 17 153 185 445 1498
Fragments arranged by size:
3150 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3381 4949 6661
Size: 274 80 33 51 1443 1404 1568 1712
Cuts at: 6661 96
Size: 138
Fragments arranged by size:
1712 1568 1443 1404 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6663 6695 6663
Size: 32 6671
Tth111I GACn'n_nGTC
Cuts at: 1316 3520 1316
Size: 2204 4499
XbaI T'CTAG_A
Cuts at: 4039 4039
Size: 6703
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6703
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6703
XmaI C'CCGG_G
Cuts at: 1870 4028 1870
Size: 2158 4545
XmnI GAAnn'nnTTC
Cuts at: 3820 4832 3820
Size: 1012 5691
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4511 276
Size: 53 83 186 936 2977 2468
Fragments arranged by size:
2977 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI