Restriction map for
pFN21AE3265
(Circular) (Six-base) MAPSORT of: pFN21AE3265.seq Check: 2336 from: 1 to: 6637
pFN21AE3265 6637 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4447 278
Size: 53 83 186 936 2911 2468
Fragments arranged by size:
2911 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3980 1937
Size: 2043 4594
Acc65I G'GTAC_C
Cuts at: 3958 3958
Size: 6637
AclI AA'CG_TT
Cuts at: 1563 3535 4764 5137 1563
Size: 1972 1229 373 3063
Fragments arranged by size:
3063 1972 1229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2961 4700 6084 6352 1632
Size: 1329 1739 1384 268 1917
Fragments arranged by size:
1917 1739 1384 1329 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6440
AflIII A'CryG_T
Cuts at: 1203 2180 2536 5536 6513 1203
Size: 977 356 3000 977 1327
Fragments arranged by size:
3000 1327 977 977 356
AgeI A'CCGG_T
Cuts at: 5468 5468
Size: 6637
AhdI GACnn_n'nnGTC
Cuts at: 5366 5366
Size: 6637
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6637
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6637
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2051 2083 2051
Size: 32 6605
AlwNI CAG_nnn'CTG
Cuts at: 2954 4380 5952 2954
Size: 1426 1572 3639
Fragments arranged by size:
3639 1572 1426
ApaLI G'TGCA_C
Cuts at: 4694 5850 4694
Size: 1156 5481
ApoI r'AATT_y
Cuts at: 1493 2302 3260 3946 4172 4324 6433 1493
Size: 809 958 686 226 152 2109 1697
Fragments arranged by size:
2109 1697 958 809 686 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5191 160
Size: 872 4159 1606
Fragments arranged by size:
4159 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
AvaI C'yCGr_G
Cuts at: 1870 1943 3057 3962 1870
Size: 73 1114 905 4545
Fragments arranged by size:
4545 1114 905 73
BamHI G'GATC_C
Cuts at: 2849 3967 4334 2849
Size: 1118 367 5152
Fragments arranged by size:
5152 1118 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3580 3958 5413 618
Size: 325 217 627 1793 378 1455 1842
Fragments arranged by size:
1842 1793 1455 627 378 325 217
BanII G_rGCy'C
Cuts at: 1391 2516 3956 1391
Size: 1125 1440 4072
Fragments arranged by size:
4072 1440 1125
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5666
BbvCI CC'TCA_GC
Cuts at: 3789 3789
Size: 6637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4828 4862 1903
Size: 34 2891 34 3678
Fragments arranged by size:
3678 2891 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4529 5739 4529
Size: 1210 5427
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6637
BfrBI ATG'CAT
Cuts at: 3479 3479
Size: 6637
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5248 136
Size: 107 122 71 631 605 3576 1525
Fragments arranged by size:
3576 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6632 6632
Size: 6637
BlpI GC'TnA_GC
Cuts at: 4054 4054
Size: 6637
Bme1580I G_kGCm'C
Cuts at: 1790 3585 4698 5854 1790
Size: 1795 1113 1156 2573
Fragments arranged by size:
2573 1795 1156 1113
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6637
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2991 4368 5326
Size: 416 53 83 399 24 1574 1377 958
Cuts at: 5326 442
Size: 1753
Fragments arranged by size:
1753 1574 1377 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 5297 1239
Size: 822 3236 2579
Fragments arranged by size:
3236 2579 822
Bpu10I CC'TnA_GC
Cuts at: 2254 3789 2254
Size: 1535 5102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2365 4762 5627 5925 2365
Size: 2397 865 298 3077
Fragments arranged by size:
3077 2397 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5300 882
Size: 617 3801 2219
Fragments arranged by size:
3801 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4428 493
Size: 711 3224 2702
Fragments arranged by size:
3224 2702 711
BsaBI GATnn'nnATC
Cuts at: 6631 6631
Size: 6637
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4444 4826 5461 275
Size: 53 83 186 936 2911 382 635 1451
Fragments arranged by size:
2911 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3894 5070 5468 5742 5889 6300 3894
Size: 1176 398 274 147 411 4231
Fragments arranged by size:
4231 1176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2051 2081 2977 3007 1178
Size: 30 165 30 648 30 896 30 4808
Fragments arranged by size:
4808 896 648 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6637
BseYI C'CCAG_C
Cuts at: 5840 6336 5840
Size: 496 6141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2217 1163
Size: 573 481 5583
Fragments arranged by size:
5583 573 481
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4087 4848 5876 1540
Size: 275 186 2086 761 1028 2301
Fragments arranged by size:
2301 2086 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3064 4698 4783 5854 3064
Size: 1634 85 1071 3847
Fragments arranged by size:
3847 1634 1071 85
BsmI GAATG_Cn'
Cuts at: 4148 4241 4148
Size: 93 6544
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2516 3064 3585 3956 4698 4783 5854
Size: 399 726 548 521 371 742 85 1071
Cuts at: 5854 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 726 548 521 399 371
85
BspEI T'CCGG_A
Cuts at: 3894 6300 3894
Size: 2406 4231
BspHI T'CATG_A
Cuts at: 4526 6256 4526
Size: 1730 4907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3992 844
Size: 3148 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3136 4524 1394
Size: 429 1313 1388 3507
Fragments arranged by size:
3507 1388 1313 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5132 5306 65
Size: 1183 3884 174 1396
Fragments arranged by size:
3884 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5281 5468 1700
Size: 110 3471 187 2869
Fragments arranged by size:
3471 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6637
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6637
BssSI C'ACGA_G
Cuts at: 3063 4697 5709 3063
Size: 1634 1012 3991
Fragments arranged by size:
3991 1634 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3883 794
Size: 3089 3548
BstBI TT'CG_AA
Cuts at: 5441 5441
Size: 6637
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6637
BstXI CCAn_nnnn'nTGG
Cuts at: 3592 3592
Size: 6637
BstYI r'GATC_y
Cuts at: 1972 2765 2849 3915 3967 4334 4719 4736 6188
Size: 793 84 1066 52 367 385 17 1452
Cuts at: 6188 6274 6286 6632 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1066 793 385 367 346 86
84 52 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3657 513
Size: 552 939 1653 3493
Fragments arranged by size:
3493 1653 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5898
Fragments arranged by size:
5898 683 56
BtsI GCAGTG_nn'
Cuts at: 2422 3534 4153 4947 4967 2422
Size: 1112 619 794 20 4092
Fragments arranged by size:
4092 1112 794 619 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6637
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6602
DraI TTT'AAA
Cuts at: 2710 3941 2710
Size: 1231 5406
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5644 809
Size: 507 4328 1802
Fragments arranged by size:
4328 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2275 2290 4084
Size: 54 1425 50 275 18 445 15 1794
Cuts at: 4084 4973 6602 8
Size: 889 1629 43
Fragments arranged by size:
1794 1629 1425 889 445 275 54 50
43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 4084 1537
Size: 275 2272 4090
Fragments arranged by size:
4090 2272 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2547 2874 4567 1353
Size: 1194 327 1693 3423
Fragments arranged by size:
3423 1693 1194 327
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5224 5598 5744 387
Size: 1064 138 3635 374 146 1280
Fragments arranged by size:
3635 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2961 4700 5297 6084 6352 1239
Size: 393 429 900 1739 597 787 268 1524
Fragments arranged by size:
1739 1524 900 787 597 429 393 268
EcoRI G'AATT_C
Cuts at: 3946 4324 3946
Size: 378 6259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2217 2343 2496 2217
Size: 126 153 6358
Fragments arranged by size:
6358 153 126
FspI TGC'GCA
Cuts at: 5143 5143
Size: 6637
HaeII r_GCGC'y
Cuts at: 1678 5784 1678
Size: 4106 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2741 2773 2977 3009 5278
Size: 32 135 32 1169 32 204 32 2269
Cuts at: 5278 5310 5352 5384 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2269 1169 204 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3568 3981 4227 669
Size: 988 281 1630 413 246 3079
Fragments arranged by size:
3079 1630 988 413 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6637
HpaI GTT'AAC
Cuts at: 4227 4227
Size: 6637
KpnI G_GTAC'C
Cuts at: 3962 3962
Size: 6637
MfeI C'AATT_G
Cuts at: 4236 4236
Size: 6637
MluI A'CGCG_T
Cuts at: 6513 6513
Size: 6637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3014 3518 5751 5935 1305
Size: 1709 504 2233 184 2007
Fragments arranged by size:
2233 2007 1709 504 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2277 2292 10
Size: 54 1425 343 445 15 4355
Fragments arranged by size:
4355 1425 445 343 54 15
MslI CAynn'nnrTG
Cuts at: 518 1166 2427 3174 3431 4595 4954 5113 518
Size: 648 1261 747 257 1164 359 159 2042
Fragments arranged by size:
2042 1261 1164 747 648 359 257 159
MspA1I CmG'CkG
Cuts at: 1606 2593 2797 4053 4730 5878 6123 1606
Size: 987 204 1256 677 1148 245 2120
Fragments arranged by size:
2120 1256 1148 987 677 245 204
NcoI C'CATG_G
Cuts at: 513 1065 2004 3657 513
Size: 552 939 1653 3493
Fragments arranged by size:
3493 1653 939 552
NdeI CA'TA_TG
Cuts at: 387 2440 387
Size: 2053 4584
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6637
NotI GC'GGCC_GC
Cuts at: 4084 4084
Size: 6637
NsiI A_TGCA'T
Cuts at: 3481 3481
Size: 6637
NspI r_CATG'y
Cuts at: 1132 1229 2184 2540 3479 3886 3995 5540 1132
Size: 97 955 356 939 407 109 1545 2229
Fragments arranged by size:
2229 1545 955 939 407 356 109 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6637
PciI A'CATG_T
Cuts at: 2180 2536 5536 2180
Size: 356 3000 3281
Fragments arranged by size:
3281 3000 356
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3753 1108
Size: 2645 3992
PmeI GTTT'AAAC
Cuts at: 3941 3941
Size: 6637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2051 2083 4706 4738 6219 6251 2051
Size: 32 2623 32 1481 32 2437
Fragments arranged by size:
2623 2437 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6637
PsiI TTA'TAA
Cuts at: 2205 4207 2205
Size: 2002 4635
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3132 3164 3132
Size: 32 6605
PstI C_TGCA'G
Cuts at: 830 3686 3989 830
Size: 2856 303 3478
Fragments arranged by size:
3478 2856 303
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6637
PvuII CAG'CTG
Cuts at: 2593 2797 2593
Size: 204 6433
SalI G'TCGA_C
Cuts at: 1936 3979 1936
Size: 2043 4594
SbfI CC_TGCA'GG
Cuts at: 3989 3989
Size: 6637
ScaI AGT'ACT
Cuts at: 4885 6597 4885
Size: 1712 4925
SfcI C'TryA_G
Cuts at: 826 1045 2597 2625 3682 3985 5120 5801 5992
Size: 219 1552 28 1057 303 1135 681 191
Cuts at: 5992 826
Size: 1471
Fragments arranged by size:
1552 1471 1135 1057 681 303 219 191
28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6637
SmaI CCC'GGG
Cuts at: 1872 3964 1872
Size: 2092 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2380 3057 4741 5642 5904 820
Size: 197 926 437 677 1684 901 262 1553
Fragments arranged by size:
1684 1553 926 901 677 437 262 197
SnaBI TAC'GTA
Cuts at: 493 4428 493
Size: 3935 2702
SpeI A'CTAG_T
Cuts at: 152 3041 152
Size: 2889 3748
SphI G_CATG'C
Cuts at: 1132 3479 3995 1132
Size: 2347 516 3774
Fragments arranged by size:
3774 2347 516
SspI AAT'ATT
Cuts at: 5 52 3640 4561 5
Size: 47 3588 921 2081
Fragments arranged by size:
3588 2081 921 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6637
StyI C'CwwG_G
Cuts at: 513 1065 2004 3657 4076 513
Size: 552 939 1653 419 3074
Fragments arranged by size:
3074 1653 939 552 419
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2184 3650 4664 4681 4834 5019 5464
Size: 1255 604 1466 1014 17 153 185 445
Cuts at: 5464 325
Size: 1498
Fragments arranged by size:
1498 1466 1255 1014 604 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2543 3148 3598
Size: 274 80 33 51 1443 566 605 450
Cuts at: 3598 4883 6595 96
Size: 1285 1712 138
Fragments arranged by size:
1712 1443 1285 605 566 450 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2632 2664 6597 6629 2632
Size: 32 3933 32 2640
Fragments arranged by size:
3933 2640 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6637
XbaI T'CTAG_A
Cuts at: 3973 3973
Size: 6637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2285 2478 2285
Size: 193 6444
XhoI C'TCGA_G
Cuts at: 1943 3057 1943
Size: 1114 5523
XmaI C'CCGG_G
Cuts at: 1870 3962 1870
Size: 2092 4545
XmnI GAAnn'nnTTC
Cuts at: 3345 4766 3345
Size: 1421 5216
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4445 276
Size: 53 83 186 936 2911 2468
Fragments arranged by size:
2911 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI