Restriction map for
pFN21AE3263
(Circular) (Six-base) MAPSORT of: pFN21AE3263.seq Check: 2797 from: 1 to: 6607
pFN21AE3263 6607 bp
With 182 enzymes: SgfI *
March 28, 2012 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4417 278
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2664 3950 1937
Size: 727 1286 4594
Fragments arranged by size:
4594 1286 727
Acc65I G'GTAC_C
Cuts at: 2529 3928 2529
Size: 1399 5208
AclI AA'CG_TT
Cuts at: 1563 4734 5107 1563
Size: 3171 373 3063
Fragments arranged by size:
3171 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2411 2925 2961 3159 3227 4670 6054 6322
Size: 779 514 36 198 68 1443 1384 268
Cuts at: 6322 1632
Size: 1917
Fragments arranged by size:
1917 1443 1384 779 514 268 198 68
36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6410
AflIII A'CryG_T
Cuts at: 1203 3838 3840 5506 6483 1203
Size: 2635 2 1666 977 1327
Fragments arranged by size:
2635 1666 1327 977 2
AgeI A'CCGG_T
Cuts at: 5438 5438
Size: 6607
AhdI GACnn_n'nnGTC
Cuts at: 5336 5336
Size: 6607
AleI CACnn'nnGTG
Cuts at: 1166 2745 1166
Size: 1579 5028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6607
AlwNI CAG_nnn'CTG
Cuts at: 3392 4350 5922 3392
Size: 958 1572 4077
Fragments arranged by size:
4077 1572 958
ApaLI G'TGCA_C
Cuts at: 2416 4664 5820 2416
Size: 2248 1156 3203
Fragments arranged by size:
3203 2248 1156
ApoI r'AATT_y
Cuts at: 1493 2159 2196 2314 3265 3462 3486 3916 4142
Size: 666 37 118 951 197 24 430 226
Cuts at: 4142 4294 6403 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 951 666 430 226 197 152
118 37 24
AseI AT'TA_AT
Cuts at: 160 1032 5161 160
Size: 872 4129 1606
Fragments arranged by size:
4129 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
AvaI C'yCGr_G
Cuts at: 1870 1943 3932 1870
Size: 73 1989 4545
Fragments arranged by size:
4545 1989 73
BamHI G'GATC_C
Cuts at: 2676 3937 4304 2676
Size: 1261 367 4979
Fragments arranged by size:
4979 1261 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2529 3928 5383 618
Size: 325 217 627 251 491 1399 1455 1842
Fragments arranged by size:
1842 1455 1399 627 491 325 251 217
BanII G_rGCy'C
Cuts at: 1391 3926 1391
Size: 2535 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2084 3429 3678 928
Size: 971 185 1345 249 3857
Fragments arranged by size:
3857 1345 971 249 185
BbvCI CC'TCA_GC
Cuts at: 2798 2798
Size: 6607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4798 4832 1903
Size: 34 2861 34 3678
Fragments arranged by size:
3678 2861 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4499 5709 4499
Size: 1210 5397
BclI T'GATC_A
Cuts at: 1548 2424 2911 1548
Size: 876 487 5244
Fragments arranged by size:
5244 876 487
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5218 136
Size: 107 122 71 631 605 3546 1525
Fragments arranged by size:
3546 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3202 6602 3202
Size: 3400 3207
BlpI GC'TnA_GC
Cuts at: 4024 4024
Size: 6607
Bme1580I G_kGCm'C
Cuts at: 1790 2420 2631 4668 5824 1790
Size: 630 211 2037 1156 2573
Fragments arranged by size:
2573 2037 1156 630 211
BmgBI CAC'GTC
Cuts at: 1318 2581 1318
Size: 1263 5344
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3262 4338 5296
Size: 416 53 83 399 24 1845 1076 958
Cuts at: 5296 442
Size: 1753
Fragments arranged by size:
1845 1753 1076 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3732 3732
Size: 6607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2714 3414 3512 5267 1239
Size: 1475 700 98 1755 2579
Fragments arranged by size:
2579 1755 1475 700 98
Bpu10I CC'TnA_GC
Cuts at: 2798 2798
Size: 6607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3227 4732 5597 5895 3227
Size: 1505 865 298 3939
Fragments arranged by size:
3939 1505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2954 5270 882
Size: 617 1455 2316 2219
Fragments arranged by size:
2316 2219 1455 617
BsaAI yAC'GTr
Cuts at: 493 1204 3728 3841 4398 493
Size: 711 2524 113 557 2702
Fragments arranged by size:
2702 2524 711 557 113
BsaBI GATnn'nnATC
Cuts at: 2229 6601 2229
Size: 4372 2235
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4414 4796 5431 275
Size: 53 83 186 936 2881 382 635 1451
Fragments arranged by size:
2881 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5040 5438 5712 5859 6270 5040
Size: 398 274 147 411 5377
Fragments arranged by size:
5377 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3694 3724 1178
Size: 30 165 30 2291 30 4061
Fragments arranged by size:
4061 2291 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3191 1186
Size: 2005 4602
BseYI C'CCAG_C
Cuts at: 5810 6306 5810
Size: 496 6111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3068 1163
Size: 573 1332 4702
Fragments arranged by size:
4702 1332 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4057 4818 5846 1540
Size: 275 186 2056 761 1028 2301
Fragments arranged by size:
2301 2056 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2420 4668 4753 5824 2420
Size: 2248 85 1071 3203
Fragments arranged by size:
3203 2248 1071 85
BsmI GAATG_Cn'
Cuts at: 3793 4118 4211 3793
Size: 325 93 6189
Fragments arranged by size:
6189 325 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2420 2631 3926 4668 4753 5824 1391
Size: 399 630 211 1295 742 85 1071 2174
Fragments arranged by size:
2174 1295 1071 742 630 399 211 85
BspEI T'CCGG_A
Cuts at: 6270 6270
Size: 6607
BspHI T'CATG_A
Cuts at: 3342 4496 6226 3342
Size: 1154 1730 3723
Fragments arranged by size:
3723 1730 1154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2647 3962 844
Size: 1803 1315 3489
Fragments arranged by size:
3489 1803 1315
BsrBI CCG'CTC
Cuts at: 1394 1823 4494 1394
Size: 429 2671 3507
Fragments arranged by size:
3507 2671 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5102 5276 65
Size: 1183 3854 174 1396
Fragments arranged by size:
3854 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5251 5438 1700
Size: 110 3441 187 2869
Fragments arranged by size:
3441 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6607
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6607
BssSI C'ACGA_G
Cuts at: 4667 5679 4667
Size: 1012 5595
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6607
BstBI TT'CG_AA
Cuts at: 5411 5411
Size: 6607
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6607
BstXI CCAn_nnnn'nTGG
Cuts at: 3300 3300
Size: 6607
BstYI r'GATC_y
Cuts at: 1972 2676 3202 3238 3638 3937 4304 4689 4706
Size: 704 526 36 400 299 367 385 17
Cuts at: 4706 6158 6244 6256 6602 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 704 526 400 385 367 346
299 86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5116
Fragments arranged by size:
5116 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2111 487
Size: 683 56 885 4983
Fragments arranged by size:
4983 885 683 56
BtsI GCAGTG_nn'
Cuts at: 2727 2789 4123 4917 4937 2727
Size: 62 1334 794 20 4397
Fragments arranged by size:
4397 1334 794 62 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6607
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6572
DraI TTT'AAA
Cuts at: 3911 3911
Size: 6607
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2585 5614 809
Size: 507 1269 3029 1802
Fragments arranged by size:
3029 1802 1269 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4054 4943 6572
Size: 54 1425 50 275 18 2224 889 1629
Cuts at: 6572 8
Size: 43
Fragments arranged by size:
2224 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4054 1537
Size: 275 2242 4090
Fragments arranged by size:
4090 2242 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2822 2843 2879 3155 3185 3369 3423 3872
Size: 1469 21 36 276 30 184 54 449
Cuts at: 3872 4537 1353
Size: 665 3423
Fragments arranged by size:
3423 1469 665 449 276 184 54 36
30 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2035 3845 5194 5568 5714 387
Size: 1064 138 446 1810 1349 374 146 1280
Fragments arranged by size:
1810 1349 1280 1064 446 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2411 2714 2925 2961 3159 3227 3414
Size: 393 779 303 211 36 198 68 187
Cuts at: 3414 3512 4670 5267 6054 6322 1239
Size: 98 1158 597 787 268 1524
Fragments arranged by size:
1524 1158 787 779 597 393 303 268
211 198 187 98 68 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2975 2975
Size: 6607
EcoO109I rG'GnC_Cy
Cuts at: 2511 3123 3518 2511
Size: 612 395 5600
Fragments arranged by size:
5600 612 395
EcoRI G'AATT_C
Cuts at: 3916 4294 3916
Size: 378 6229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2569 2569
Size: 6607
FspI TGC'GCA
Cuts at: 5113 5113
Size: 6607
HaeII r_GCGC'y
Cuts at: 1678 5754 1678
Size: 4076 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2921 2953 3395 3427 3565
Size: 32 135 32 1349 32 442 32 138
Cuts at: 3565 3597 5248 5280 5322 5354 1373
Size: 32 1651 32 42 32 2626
Fragments arranged by size:
2626 1651 1349 442 138 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3592 3951 4197 669
Size: 988 281 1654 359 246 3079
Fragments arranged by size:
3079 1654 988 359 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6607
HpaI GTT'AAC
Cuts at: 4197 4197
Size: 6607
KpnI G_GTAC'C
Cuts at: 2533 3932 2533
Size: 1399 5208
MfeI C'AATT_G
Cuts at: 4206 4206
Size: 6607
MluI A'CGCG_T
Cuts at: 6483 6483
Size: 6607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2120 3469 5721 5905 1305
Size: 815 1349 2252 184 2007
Fragments arranged by size:
2252 2007 1349 815 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4785
Fragments arranged by size:
4785 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2714 2745 4565 4924 5083 518
Size: 648 1548 31 1820 359 159 2042
Fragments arranged by size:
2042 1820 1548 648 359 159 31
MspA1I CmG'CkG
Cuts at: 1606 2379 2389 3389 3451 4023 4700 5848 6093
Size: 773 10 1000 62 572 677 1148 245
Cuts at: 6093 1606
Size: 2120
Fragments arranged by size:
2120 1148 1000 773 677 572 245 62
10
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5116
Fragments arranged by size:
5116 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6607
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6607
NotI GC'GGCC_GC
Cuts at: 4054 4054
Size: 6607
NspI r_CATG'y
Cuts at: 1132 1229 3965 5510 1132
Size: 97 2736 1545 2229
Fragments arranged by size:
2736 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2404 1348
Size: 1056 5551
PciI A'CATG_T
Cuts at: 5506 5506
Size: 6607
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2540 2692 1108
Size: 1432 152 5023
Fragments arranged by size:
5023 1432 152
PfoI T'CCnGG_A
Cuts at: 2679 3182 3641 2679
Size: 503 459 5645
Fragments arranged by size:
5645 503 459
PmeI GTTT'AAAC
Cuts at: 3911 3911
Size: 6607
PmlI CAC'GTG
Cuts at: 3728 3841 3728
Size: 113 6494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4676 4708 6189 6221 4676
Size: 32 1481 32 5062
Fragments arranged by size:
5062 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2511 3518 2511
Size: 1007 5600
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6607
PsiI TTA'TAA
Cuts at: 2640 4177 2640
Size: 1537 5070
PstI C_TGCA'G
Cuts at: 830 3959 830
Size: 3129 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6607
PvuII CAG'CTG
Cuts at: 2389 3389 3451 2389
Size: 1000 62 5545
Fragments arranged by size:
5545 1000 62
SalI G'TCGA_C
Cuts at: 1936 3949 1936
Size: 2013 4594
SanDI GG'GwC_CC
Cuts at: 3518 3518
Size: 6607
SapI GCTCTTCn'nnn_
Cuts at: 3369 3369
Size: 6607
SbfI CC_TGCA'GG
Cuts at: 3959 3959
Size: 6607
ScaI AGT'ACT
Cuts at: 3251 4855 6567 3251
Size: 1604 1712 3291
Fragments arranged by size:
3291 1712 1604
SfcI C'TryA_G
Cuts at: 826 1045 3955 5090 5771 5962 826
Size: 219 2910 1135 681 191 1471
Fragments arranged by size:
2910 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6607
SmaI CCC'GGG
Cuts at: 1872 3934 1872
Size: 2062 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3206 4711 5612 5874 820
Size: 197 926 1263 1505 901 262 1553
Fragments arranged by size:
1553 1505 1263 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4398 493
Size: 3905 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6607
SphI G_CATG'C
Cuts at: 1132 3965 1132
Size: 2833 3774
SspI AAT'ATT
Cuts at: 5 52 2257 2305 2922 4531 5
Size: 47 2205 48 617 1609 2081
Fragments arranged by size:
2205 2081 1609 617 48 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6607
StyI C'CwwG_G
Cuts at: 513 1065 2004 2049 4046 513
Size: 552 939 45 1997 3074
Fragments arranged by size:
3074 1997 939 552 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4634 4651 4804 4989 5434 325
Size: 1255 3054 17 153 185 445 1498
Fragments arranged by size:
3054 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3249 4853 6565
Size: 274 80 33 51 1443 1272 1604 1712
Cuts at: 6565 96
Size: 138
Fragments arranged by size:
1712 1604 1443 1272 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3045 3077 6567 6599 3045
Size: 32 3490 32 3053
Fragments arranged by size:
3490 3053 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6607
XbaI T'CTAG_A
Cuts at: 3943 3943
Size: 6607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2317 2731 2317
Size: 414 6193
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6607
XmaI C'CCGG_G
Cuts at: 1870 3932 1870
Size: 2062 4545
XmnI GAAnn'nnTTC
Cuts at: 4736 4736
Size: 6607
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4415 276
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacI SacII SexAI
SfiI SfoI SrfI SwaI