Restriction map for
pFN21AE3261
(Circular) (Six-base) MAPSORT of: pFN21AE3261.seq Check: 5653 from: 1 to: 6583
pFN21AE3261 6583 bp
With 182 enzymes: SgfI *
March 28, 2012 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2409 2409
Size: 6583
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4393 278
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3926 1937
Size: 1989 4594
Acc65I G'GTAC_C
Cuts at: 3904 3904
Size: 6583
AclI AA'CG_TT
Cuts at: 1563 4710 5083 1563
Size: 3147 373 3063
Fragments arranged by size:
3147 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3323 4646 6030 6298 1632
Size: 1691 1323 1384 268 1917
Fragments arranged by size:
1917 1691 1384 1323 268
AfeI AGC'GCT
Cuts at: 2555 2555
Size: 6583
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6386
AflIII A'CryG_T
Cuts at: 1203 5482 6459 1203
Size: 4279 977 1327
Fragments arranged by size:
4279 1327 977
AgeI A'CCGG_T
Cuts at: 5414 5414
Size: 6583
AhdI GACnn_n'nnGTC
Cuts at: 5312 5312
Size: 6583
AleI CACnn'nnGTG
Cuts at: 1166 3431 1166
Size: 2265 4318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6583
AlwNI CAG_nnn'CTG
Cuts at: 2121 4326 5898 2121
Size: 2205 1572 2806
Fragments arranged by size:
2806 2205 1572
ApaI G_GGCC'C
Cuts at: 2188 2244 3869 2188
Size: 56 1625 4902
Fragments arranged by size:
4902 1625 56
ApaLI G'TGCA_C
Cuts at: 4640 5796 4640
Size: 1156 5427
ApoI r'AATT_y
Cuts at: 1493 2330 3059 3249 3523 3892 4118 4270 6379
Size: 837 729 190 274 369 226 152 2109
Cuts at: 6379 1493
Size: 1697
Fragments arranged by size:
2109 1697 837 729 369 274 226 190
152
AseI AT'TA_AT
Cuts at: 160 1032 5137 160
Size: 872 4105 1606
Fragments arranged by size:
4105 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
AvaI C'yCGr_G
Cuts at: 1870 1943 2259 2877 3385 3597 3908 1870
Size: 73 316 618 508 212 311 4545
Fragments arranged by size:
4545 618 508 316 311 212 73
BamHI G'GATC_C
Cuts at: 3913 4280 3913
Size: 367 6216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 3368 3904 5359 618
Size: 325 217 627 224 1357 536 1455 1842
Fragments arranged by size:
1842 1455 1357 627 536 325 224 217
BanII G_rGCy'C
Cuts at: 1391 2067 2188 2244 2627 2763 2884 3869 3902
Size: 676 121 56 383 136 121 985 33
Cuts at: 3902 1391
Size: 4072
Fragments arranged by size:
4072 985 676 383 136 121 121 56
33
BbeI G_GCGC'C
Cuts at: 2015 2015
Size: 6583
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2275 928
Size: 971 376 5236
Fragments arranged by size:
5236 971 376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2796 2830 4774 4808 1903
Size: 34 859 34 1944 34 3678
Fragments arranged by size:
3678 1944 859 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2762 4475 5685 2762
Size: 1713 1210 3660
Fragments arranged by size:
3660 1713 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6583
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5194 136
Size: 107 122 71 631 605 337 3185 1525
Fragments arranged by size:
3185 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6578 6578
Size: 6583
BlpI GC'TnA_GC
Cuts at: 3035 3576 4000 3035
Size: 541 424 5618
Fragments arranged by size:
5618 541 424
Bme1580I G_kGCm'C
Cuts at: 1790 2105 2141 2188 2244 2500 3869 3878 4644
Size: 315 36 47 56 256 1369 9 766
Cuts at: 4644 5800 1790
Size: 1156 2573
Fragments arranged by size:
2573 1369 1156 766 315 256 56 47
36 9
BmgBI CAC'GTC
Cuts at: 1318 2173 3619 1318
Size: 855 1446 4282
Fragments arranged by size:
4282 1446 855
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2456 2593 2745
Size: 416 53 83 399 24 1039 137 152
Cuts at: 2745 3863 4314 5272 442
Size: 1118 451 958 1753
Fragments arranged by size:
1753 1118 1039 958 451 416 399 152
137 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2696 2778 3704 3857 5243 1239
Size: 1457 82 926 153 1386 2579
Fragments arranged by size:
2579 1457 1386 926 153 82
Bpu10I CC'TnA_GC
Cuts at: 2626 2883 2961 2626
Size: 257 78 6248
Fragments arranged by size:
6248 257 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3773 4708 5573 5871 3773
Size: 935 865 298 4485
Fragments arranged by size:
4485 935 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2414 5246 882
Size: 617 915 2832 2219
Fragments arranged by size:
2832 2219 915 617
BsaAI yAC'GTr
Cuts at: 493 1204 4374 493
Size: 711 3170 2702
Fragments arranged by size:
3170 2702 711
BsaBI GATnn'nnATC
Cuts at: 6577 6577
Size: 6583
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2280 2528 4390
Size: 53 83 186 936 479 268 248 1862
Cuts at: 4390 4772 5407 275
Size: 382 635 1451
Fragments arranged by size:
1862 1451 936 635 479 382 268 248
186 83 53
BsaWI w'CCGG_w
Cuts at: 3135 5016 5414 5688 5835 6246 3135
Size: 1881 398 274 147 411 3472
Fragments arranged by size:
3472 1881 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2067 2097 3752 3782 1178
Size: 30 165 30 664 30 1655 30 3979
Fragments arranged by size:
3979 1655 664 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 2825 3848 1186
Size: 1153 486 1023 3921
Fragments arranged by size:
3921 1153 1023 486
BseYI C'CCAG_C
Cuts at: 2134 2375 3283 5786 6282 2134
Size: 241 908 2503 496 2435
Fragments arranged by size:
2503 2435 908 496 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3631 1163
Size: 573 1895 4115
Fragments arranged by size:
4115 1895 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4033 4794 5822 1540
Size: 275 186 2032 761 1028 2301
Fragments arranged by size:
2301 2032 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2067 2763 4644 4729 5800 2067
Size: 696 1881 85 1071 2850
Fragments arranged by size:
2850 1881 1071 696 85
BsmI GAATG_Cn'
Cuts at: 4094 4187 4094
Size: 93 6490
BsmBI CGTCTCn'nnnn_
Cuts at: 2029 2161 2386 2029
Size: 132 225 6226
Fragments arranged by size:
6226 225 132
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2105 2141 2188 2244 2500 2627
Size: 399 277 38 36 47 56 256 127
Cuts at: 2627 2763 2884 3869 3878 3902 4644 4729 5800
Size: 136 121 985 9 24 742 85 1071
Cuts at: 5800 1391
Size: 2174
Fragments arranged by size:
2174 1071 985 742 399 277 256 136
127 121 85 56 47 38 36 24
9
BspEI T'CCGG_A
Cuts at: 3135 6246 3135
Size: 3111 3472
BspHI T'CATG_A
Cuts at: 4472 6202 4472
Size: 1730 4853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2409 2985 3099 3938 844
Size: 1565 576 114 839 3489
Fragments arranged by size:
3489 1565 839 576 114
BsrBI CCG'CTC
Cuts at: 1394 1823 2264 3810 4470 1394
Size: 429 441 1546 660 3507
Fragments arranged by size:
3507 1546 660 441 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5078 5252 65
Size: 1183 3830 174 1396
Fragments arranged by size:
3830 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2312 5227 5414 1700
Size: 110 502 2915 187 2869
Fragments arranged by size:
2915 2869 502 187 110
BsrGI T'GTAC_A
Cuts at: 96 2646 96
Size: 2550 4033
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6583
BssSI C'ACGA_G
Cuts at: 4643 5655 4643
Size: 1012 5571
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6583
BstBI TT'CG_AA
Cuts at: 5387 5387
Size: 6583
BstEII G'GTnAC_C
Cuts at: 1184 3652 1184
Size: 2468 4115
BstXI CCAn_nnnn'nTGG
Cuts at: 2699 3682 2699
Size: 983 5600
BstYI r'GATC_y
Cuts at: 1972 3913 4280 4665 4682 6134 6220 6232 6578
Size: 1941 367 385 17 1452 86 12 346
Cuts at: 6578 1972
Size: 1977
Fragments arranged by size:
1977 1941 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2051 2087 2425 2904 3518 2051
Size: 36 338 479 614 5116
Fragments arranged by size:
5116 614 479 338 36
BtgI C'CryG_G
Cuts at: 513 1065 2004 2244 2537 2925 3255 513
Size: 552 939 240 293 388 330 3841
Fragments arranged by size:
3841 939 552 388 330 293 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2124 487
Size: 683 56 898 4946
Fragments arranged by size:
4946 898 683 56
BtsI GCAGTG_nn'
Cuts at: 2664 2937 4099 4893 4913 2664
Size: 273 1162 794 20 4334
Fragments arranged by size:
4334 1162 794 273 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6548
DraI TTT'AAA
Cuts at: 3887 3887
Size: 6583
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5590 809
Size: 507 4274 1802
Fragments arranged by size:
4274 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2782 2893 3438
Size: 54 1425 50 275 18 952 111 545
Cuts at: 3438 3642 3805 4030 4919 6548 8
Size: 204 163 225 889 1629 43
Fragments arranged by size:
1629 1425 952 889 545 275 225 204
163 111 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4030 1537
Size: 275 2218 4090
Fragments arranged by size:
4090 2218 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3207 4513 1353
Size: 1854 1306 3423
Fragments arranged by size:
3423 1854 1306
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2285 3428 3512 5170 5544 5690
Size: 1064 138 696 1143 84 1658 374 146
Cuts at: 5690 387
Size: 1280
Fragments arranged by size:
1658 1280 1143 1064 696 374 146 138
84
EcoICRI GAG'CTC
Cuts at: 2065 2761 2065
Size: 696 5887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2696 2778 3323 3704 3857 4646 5243
Size: 393 1064 82 545 381 153 789 597
Cuts at: 5243 6030 6298 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1064 789 787 597 545 393 381
268 153 82
EcoNI CCTnn'n_nnAGG
Cuts at: 2564 3362 2564
Size: 798 5785
EcoO109I rG'GnC_Cy
Cuts at: 2091 2184 2185 2240 2267 2706 2887 3508 3849
Size: 93 1 55 27 439 181 621 341
Cuts at: 3849 2091
Size: 4825
Fragments arranged by size:
4825 621 439 341 181 93 55 27
1
EcoRI G'AATT_C
Cuts at: 3249 3892 4270 3249
Size: 643 378 5562
Fragments arranged by size:
5562 643 378
FspI TGC'GCA
Cuts at: 5089 5089
Size: 6583
HaeII r_GCGC'y
Cuts at: 1678 2015 2319 2557 5730 1678
Size: 337 304 238 3173 2531
Fragments arranged by size:
3173 2531 337 304 238
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2061 2067 2093 2099 3574
Size: 32 135 32 489 6 26 6 1475
Cuts at: 3574 3606 5224 5256 5298 5330 1373
Size: 32 1618 32 42 32 2626
Fragments arranged by size:
2626 1618 1475 489 135 42 32 32
32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3130 3927 4173 669
Size: 988 281 1192 797 246 3079
Fragments arranged by size:
3079 1192 988 797 281 246
HindIII A'AGCT_T
Cuts at: 748 3689 3749 748
Size: 2941 60 3582
Fragments arranged by size:
3582 2941 60
HpaI GTT'AAC
Cuts at: 4173 4173
Size: 6583
KasI G'GCGC_C
Cuts at: 2011 2011
Size: 6583
KpnI G_GTAC'C
Cuts at: 3908 3908
Size: 6583
MfeI C'AATT_G
Cuts at: 4182 4182
Size: 6583
MluI A'CGCG_T
Cuts at: 6459 6459
Size: 6583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2836 3103 3774 5697 5881 1305
Size: 1531 267 671 1923 184 2007
Fragments arranged by size:
2007 1923 1531 671 267 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2895 3644 10
Size: 54 1425 343 1063 749 2949
Fragments arranged by size:
2949 1425 1063 749 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2924 3431 3545 3650 3788 4541 4900
Size: 648 1758 507 114 105 138 753 359
Cuts at: 4900 5059 518
Size: 159 2042
Fragments arranged by size:
2042 1758 753 648 507 359 159 138
114 105
MspA1I CmG'CkG
Cuts at: 1606 2217 2223 2246 3403 3463 3999 4676 5824
Size: 611 6 23 1157 60 536 677 1148
Cuts at: 5824 6069 1606
Size: 245 2120
Fragments arranged by size:
2120 1157 1148 677 611 536 245 60
23 6
NaeI GCC'GGC
Cuts at: 2314 2314
Size: 6583
NarI GG'CG_CC
Cuts at: 2012 2012
Size: 6583
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5092
Fragments arranged by size:
5092 939 552
NdeI CA'TA_TG
Cuts at: 387 2970 387
Size: 2583 4000
NgoMIV G'CCGG_C
Cuts at: 2312 2312
Size: 6583
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6583
NotI GC'GGCC_GC
Cuts at: 4030 4030
Size: 6583
NruI TCG'CGA
Cuts at: 2109 2109
Size: 6583
NspI r_CATG'y
Cuts at: 1132 1229 2519 3298 3941 5486 1132
Size: 97 1290 779 643 1545 2229
Fragments arranged by size:
2229 1545 1290 779 643 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6583
PciI A'CATG_T
Cuts at: 5482 5482
Size: 6583
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2391 3571 1108
Size: 1283 1180 4120
Fragments arranged by size:
4120 1283 1180
PfoI T'CCnGG_A
Cuts at: 2673 3834 2673
Size: 1161 5422
PmeI GTTT'AAAC
Cuts at: 3887 3887
Size: 6583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4652 4684 6165 6197 4652
Size: 32 1481 32 5038
Fragments arranged by size:
5038 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2267 2887 2267
Size: 620 5963
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6583
PsiI TTA'TAA
Cuts at: 4153 4153
Size: 6583
PspOMI G'GGCC_C
Cuts at: 2184 2240 3865 2184
Size: 56 1625 4902
Fragments arranged by size:
4902 1625 56
PspXI vC'TCGA_Gb
Cuts at: 3385 3385
Size: 6583
PstI C_TGCA'G
Cuts at: 830 2222 3408 3507 3935 830
Size: 1392 1186 99 428 3478
Fragments arranged by size:
3478 1392 1186 428 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6583
PvuII CAG'CTG
Cuts at: 3403 3403
Size: 6583
SacI G_AGCT'C
Cuts at: 2067 2763 2067
Size: 696 5887
SacII CC_GC'GG
Cuts at: 2247 2247
Size: 6583
SalI G'TCGA_C
Cuts at: 1936 3925 1936
Size: 1989 4594
SbfI CC_TGCA'GG
Cuts at: 3935 3935
Size: 6583
ScaI AGT'ACT
Cuts at: 4831 6543 4831
Size: 1712 4871
SexAI A'CCwGG_T
Cuts at: 2850 2850
Size: 6583
SfcI C'TryA_G
Cuts at: 826 1045 2218 3404 3503 3931 5066 5747 5938
Size: 219 1173 1186 99 428 1135 681 191
Cuts at: 5938 826
Size: 1471
Fragments arranged by size:
1471 1186 1173 1135 681 428 219 191
99
SfoI GGC'GCC
Cuts at: 2013 2013
Size: 6583
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
SgrAI Cr'CCGG_yG
Cuts at: 1700 2312 1700
Size: 612 5971
SmaI CCC'GGG
Cuts at: 1872 3599 3910 1872
Size: 1727 311 4545
Fragments arranged by size:
4545 1727 311
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3385 3752 4687 5588 5850 820
Size: 197 926 1442 367 935 901 262 1553
Fragments arranged by size:
1553 1442 935 926 901 367 262 197
SnaBI TAC'GTA
Cuts at: 493 4374 493
Size: 3881 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6583
SphI G_CATG'C
Cuts at: 1132 3941 1132
Size: 2809 3774
SrfI GCCC'GGGC
Cuts at: 3599 3599
Size: 6583
SspI AAT'ATT
Cuts at: 5 52 4507 5
Size: 47 4455 2081
Fragments arranged by size:
4455 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6583
StyI C'CwwG_G
Cuts at: 513 1065 2004 2148 2773 4022 513
Size: 552 939 144 625 1249 3074
Fragments arranged by size:
3074 1249 939 625 552 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2165 4610 4627 4780 4965 5410 325
Size: 1255 585 2445 17 153 185 445 1498
Fragments arranged by size:
2445 1498 1255 585 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2646 4829 6541
Size: 274 80 33 51 1443 669 2183 1712
Cuts at: 6541 96
Size: 138
Fragments arranged by size:
2183 1712 1443 669 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3240 3272 6543 6575 3240
Size: 32 3271 32 3248
Fragments arranged by size:
3271 3248 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2687 1316
Size: 1371 5212
XbaI T'CTAG_A
Cuts at: 2821 3112 3919 2821
Size: 291 807 5485
Fragments arranged by size:
5485 807 291
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3027 3193 3790 3027
Size: 166 597 5820
Fragments arranged by size:
5820 597 166
XhoI C'TCGA_G
Cuts at: 1943 3385 1943
Size: 1442 5141
XmaI C'CCGG_G
Cuts at: 1870 3597 3908 1870
Size: 1727 311 4545
Fragments arranged by size:
4545 1727 311
XmnI GAAnn'nnTTC
Cuts at: 2333 4712 2333
Size: 2379 4204
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4391 276
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FalI FseI FspAI NsiI PacI
PmlI PsrI RsrII SanDI SapI SfiI SwaI