Restriction map for
pFN21AE3256
(Circular) (Six-base) MAPSORT of: pFN21AE3256.seq Check: 6192 from: 1 to: 6496
pFN21AE3256 6496 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4306 278
Size: 53 83 186 936 2770 2468
Fragments arranged by size:
2770 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2916 3839 1937
Size: 979 923 4594
Fragments arranged by size:
4594 979 923
Acc65I G'GTAC_C
Cuts at: 3817 3817
Size: 6496
AclI AA'CG_TT
Cuts at: 1563 4623 4996 1563
Size: 3060 373 3063
Fragments arranged by size:
3063 3060 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2266 2431 3131 4559 5943 6211 1632
Size: 634 165 700 1428 1384 268 1917
Fragments arranged by size:
1917 1428 1384 700 634 268 165
AfeI AGC'GCT
Cuts at: 3185 3185
Size: 6496
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6299
AflIII A'CryG_T
Cuts at: 1203 3453 3508 5395 6372 1203
Size: 2250 55 1887 977 1327
Fragments arranged by size:
2250 1887 1327 977 55
AgeI A'CCGG_T
Cuts at: 2849 5327 2849
Size: 2478 4018
AhdI GACnn_n'nnGTC
Cuts at: 3346 5225 3346
Size: 1879 4617
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6496
AlwNI CAG_nnn'CTG
Cuts at: 2272 2665 4239 5811 2272
Size: 393 1574 1572 2957
Fragments arranged by size:
2957 1574 1572 393
ApaI G_GGCC'C
Cuts at: 2820 2820
Size: 6496
ApaLI G'TGCA_C
Cuts at: 4553 5709 4553
Size: 1156 5340
ApoI r'AATT_y
Cuts at: 1493 3805 4031 4183 6292 1493
Size: 2312 226 152 2109 1697
Fragments arranged by size:
2312 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2540 2540
Size: 6496
AseI AT'TA_AT
Cuts at: 160 1032 5050 160
Size: 872 4018 1606
Fragments arranged by size:
4018 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
AvaI C'yCGr_G
Cuts at: 1870 1943 2455 3706 3821 1870
Size: 73 512 1251 115 4545
Fragments arranged by size:
4545 1251 512 115 73
BamHI G'GATC_C
Cuts at: 3826 4193 3826
Size: 367 6129
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2510 3100 3817 5272
Size: 325 217 627 230 493 590 717 1455
Cuts at: 5272 618
Size: 1842
Fragments arranged by size:
1842 1455 717 627 590 493 325 230
217
BanII G_rGCy'C
Cuts at: 1391 2820 3815 1391
Size: 1429 995 4072
Fragments arranged by size:
4072 1429 995
BbeI G_GCGC'C
Cuts at: 2021 2514 3104 2021
Size: 493 590 5413
Fragments arranged by size:
5413 590 493
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5525
BbvCI CC'TCA_GC
Cuts at: 2439 3444 2439
Size: 1005 5491
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4687 4721 1903
Size: 34 2750 34 3678
Fragments arranged by size:
3678 2750 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3554 4388 5598 3554
Size: 834 1210 4452
Fragments arranged by size:
4452 1210 834
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6496
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2499 5107 136
Size: 107 122 71 631 605 827 2608 1525
Fragments arranged by size:
2608 1525 827 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6491 6491
Size: 6496
BlpI GC'TnA_GC
Cuts at: 2322 3913 2322
Size: 1591 4905
Bme1580I G_kGCm'C
Cuts at: 1790 2583 2820 4557 5713 1790
Size: 793 237 1737 1156 2573
Fragments arranged by size:
2573 1737 1156 793 237
BmgBI CAC'GTC
Cuts at: 1318 2113 2716 3454 3589 1318
Size: 795 603 738 135 4225
Fragments arranged by size:
4225 795 738 603 135
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4227 5185 442
Size: 416 53 83 399 24 2810 958 1753
Fragments arranged by size:
2810 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2543 2687 5156 1239
Size: 902 402 144 2469 2579
Fragments arranged by size:
2579 2469 902 402 144
Bpu10I CC'TnA_GC
Cuts at: 2060 2078 2439 3444 2060
Size: 18 361 1005 5112
Fragments arranged by size:
5112 1005 361 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3058 4621 5486 5784 3058
Size: 1563 865 298 3770
Fragments arranged by size:
3770 1563 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2858 3299 5159 882
Size: 617 1359 441 1860 2219
Fragments arranged by size:
2219 1860 1359 617 441
BsaAI yAC'GTr
Cuts at: 493 1204 2497 3019 3160 4287 493
Size: 711 1293 522 141 1127 2702
Fragments arranged by size:
2702 1293 1127 711 522 141
BsaBI GATnn'nnATC
Cuts at: 6490 6490
Size: 6496
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2511 3101 4303
Size: 53 83 186 936 485 493 590 1202
Cuts at: 4303 4685 5320 275
Size: 382 635 1451
Fragments arranged by size:
1451 1202 936 635 590 493 485 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2849 4929 5327 5601 5748 6159 2849
Size: 2080 398 274 147 411 3186
Fragments arranged by size:
3186 2080 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3116 3146 3233 3263 1178
Size: 30 165 30 1713 30 87 30 4411
Fragments arranged by size:
4411 1713 165 87 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2603 3124 1186
Size: 1417 521 4558
Fragments arranged by size:
4558 1417 521
BseYI C'CCAG_C
Cuts at: 2506 2616 3001 5699 6195 2506
Size: 110 385 2698 496 2807
Fragments arranged by size:
2807 2698 496 385 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2225 3776 1163
Size: 573 489 1551 3883
Fragments arranged by size:
3883 1551 573 489
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2015 2364 2803 2812 3770 3946
Size: 275 186 14 349 439 9 958 176
Cuts at: 3946 4707 5735 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 958 761 439 349 275 186
176 14 9
BsiHKAI G_wGCw'C
Cuts at: 2874 4557 4642 5713 2874
Size: 1683 85 1071 3657
Fragments arranged by size:
3657 1683 1071 85
BsmI GAATG_Cn'
Cuts at: 2835 4007 4100 2835
Size: 1172 93 5231
Fragments arranged by size:
5231 1172 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3556 3556
Size: 6496
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2583 2820 2874 3815 4557 4642 5713
Size: 399 793 237 54 941 742 85 1071
Cuts at: 5713 1391
Size: 2174
Fragments arranged by size:
2174 1071 941 793 742 399 237 85
54
BspEI T'CCGG_A
Cuts at: 6159 6159
Size: 6496
BspHI T'CATG_A
Cuts at: 4385 6115 4385
Size: 1730 4766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2355 2565 3851 844
Size: 1511 210 1286 3489
Fragments arranged by size:
3489 1511 1286 210
BsrBI CCG'CTC
Cuts at: 1394 1823 2230 2527 4383 1394
Size: 429 407 297 1856 3507
Fragments arranged by size:
3507 1856 429 407 297
BsrDI GCAATG_nn'
Cuts at: 65 1248 3013 4991 5165 65
Size: 1183 1765 1978 174 1396
Fragments arranged by size:
1978 1765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2849 3270 5140 5327 1700
Size: 110 204 835 421 1870 187 2869
Fragments arranged by size:
2869 1870 835 421 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 3456 96
Size: 3360 3136
BssHII G'CGCG_C
Cuts at: 1925 2089 2335 2540 2738 2774 2792 1925
Size: 164 246 205 198 36 18 5629
Fragments arranged by size:
5629 246 205 198 164 36 18
BssSI C'ACGA_G
Cuts at: 2867 2873 4556 5568 2867
Size: 6 1683 1012 3795
Fragments arranged by size:
3795 1683 1012 6
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2248 2272 794
Size: 1454 24 5018
Fragments arranged by size:
5018 1454 24
BstBI TT'CG_AA
Cuts at: 5300 5300
Size: 6496
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6496
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 2419
Size: 6496
BstYI r'GATC_y
Cuts at: 1972 2862 3486 3826 4193 4578 4595 6047 6133
Size: 890 624 340 367 385 17 1452 86
Cuts at: 6133 6145 6491 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 890 624 385 367 346 340
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2812 3237 3265 3309 3773 513
Size: 552 939 808 425 28 44 464 3236
Fragments arranged by size:
3236 939 808 552 464 425 44 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3756 487
Size: 683 56 2530 3227
Fragments arranged by size:
3227 2530 683 56
BtsI GCAGTG_nn'
Cuts at: 3255 3326 3673 4012 4806 4826 3255
Size: 71 347 339 794 20 4925
Fragments arranged by size:
4925 794 347 339 71 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6496
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6461
DraI TTT'AAA
Cuts at: 3800 3800
Size: 6496
DraIII CAC_nnn'GTG
Cuts at: 2248 3261 2248
Size: 1013 5483
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2906 3028 5503 809
Size: 507 1590 122 2475 1802
Fragments arranged by size:
2475 1802 1590 507 122
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2166 2361
Size: 54 1425 50 275 18 195 141 195
Cuts at: 2361 2409 2654 2955 3165 3268 3364 3525 3767
Size: 48 245 301 210 103 96 161 242
Cuts at: 3767 3943 4832 6461 8
Size: 176 889 1629 43
Fragments arranged by size:
1629 1425 889 301 275 245 242 210
195 195 176 161 141 103 96 54
50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2361 3767 3943 1537
Size: 275 549 1406 176 4090
Fragments arranged by size:
4090 1406 549 275 176
EarI CTCTTCn'nnn_
Cuts at: 1353 4426 1353
Size: 3073 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2994 3107 3671 5083 5457 5603
Size: 1064 138 1405 113 564 1412 374 146
Cuts at: 5603 387
Size: 1280
Fragments arranged by size:
1412 1405 1280 1064 564 374 146 138
113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2266 2431 2543 2687 3131 4559
Size: 393 509 125 165 112 144 444 1428
Cuts at: 4559 5156 5943 6211 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1428 787 597 509 444 393 268
165 144 125 112
EcoO109I rG'GnC_Cy
Cuts at: 2382 2817 3214 3778 2382
Size: 435 397 564 5100
Fragments arranged by size:
5100 564 435 397
EcoRI G'AATT_C
Cuts at: 3805 4183 3805
Size: 378 6118
FspI TGC'GCA
Cuts at: 5002 5002
Size: 6496
HaeII r_GCGC'y
Cuts at: 1678 2021 2514 2612 2950 3002 3104 3187 5643
Size: 343 493 98 338 52 102 83 2456
Cuts at: 5643 1678
Size: 2531
Fragments arranged by size:
2531 2456 493 343 338 102 98 83
52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3233 3265 3332 3364 3388
Size: 32 135 32 1661 32 67 32 24
Cuts at: 3388 3420 5137 5169 5211 5243 1373
Size: 32 1717 32 42 32 2626
Fragments arranged by size:
2626 1717 1661 135 67 42 32 32
32 32 32 32 32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 2917 3840 4086 669
Size: 988 281 979 923 246 3079
Fragments arranged by size:
3079 988 979 923 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6496
HpaI GTT'AAC
Cuts at: 4086 4086
Size: 6496
KasI G'GCGC_C
Cuts at: 2017 2510 3100 2017
Size: 493 590 5413
Fragments arranged by size:
5413 590 493
KpnI G_GTAC'C
Cuts at: 3821 3821
Size: 6496
MfeI C'AATT_G
Cuts at: 4095 4095
Size: 6496
MluI A'CGCG_T
Cuts at: 6372 6372
Size: 6496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3708 5610 5794 1305
Size: 2403 1902 184 2007
Fragments arranged by size:
2403 2007 1902 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2411 10
Size: 54 1425 343 579 4095
Fragments arranged by size:
4095 1425 579 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3017 3314 3666 4454 4813 4972 518
Size: 648 1851 297 352 788 359 159 2042
Fragments arranged by size:
2042 1851 788 648 359 352 297 159
MspA1I CmG'CkG
Cuts at: 1606 2086 2209 2269 2329 2451 2479 2521 2605
Size: 480 123 60 60 122 28 42 84
Cuts at: 2605 2662 2731 2814 3443 3775 3912 4589 5737
Size: 57 69 83 629 332 137 677 1148
Cuts at: 5737 5982 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 629 480 332 245 137
123 122 84 83 69 60 60 57
42 28
NaeI GCC'GGC
Cuts at: 3272 3272
Size: 6496
NarI GG'CG_CC
Cuts at: 2018 2511 3101 2018
Size: 493 590 5413
Fragments arranged by size:
5413 590 493
NcoI C'CATG_G
Cuts at: 513 1065 2004 3237 3309 513
Size: 552 939 1233 72 3700
Fragments arranged by size:
3700 1233 939 552 72
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6496
NgoMIV G'CCGG_C
Cuts at: 3270 3270
Size: 6496
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6496
NotI GC'GGCC_GC
Cuts at: 3943 3943
Size: 6496
NspI r_CATG'y
Cuts at: 1132 1229 2263 2428 3512 3854 5399 1132
Size: 97 1034 165 1084 342 1545 2229
Fragments arranged by size:
2229 1545 1084 1034 342 165 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6496
PciI A'CATG_T
Cuts at: 3508 5395 3508
Size: 1887 4609
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3657 1108
Size: 2549 3947
PfoI T'CCnGG_A
Cuts at: 2106 2106
Size: 6496
PmeI GTTT'AAAC
Cuts at: 3800 3800
Size: 6496
PmlI CAC'GTG
Cuts at: 2497 2497
Size: 6496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2087 2119 4565 4597 6078 6110 2087
Size: 32 2446 32 1481 32 2473
Fragments arranged by size:
2473 2446 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3778 3778
Size: 6496
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6496
PsiI TTA'TAA
Cuts at: 4066 4066
Size: 6496
PspOMI G'GGCC_C
Cuts at: 2816 2816
Size: 6496
PstI C_TGCA'G
Cuts at: 830 2268 2355 2394 3222 3848 830
Size: 1438 87 39 828 626 3478
Fragments arranged by size:
3478 1438 828 626 87 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6496
PvuII CAG'CTG
Cuts at: 2086 2209 2269 2451 2521 2605 2662 2086
Size: 123 60 182 70 84 57 5920
Fragments arranged by size:
5920 182 123 84 70 60 57
SacII CC_GC'GG
Cuts at: 2815 3776 2815
Size: 961 5535
SalI G'TCGA_C
Cuts at: 1936 2915 3838 1936
Size: 979 923 4594
Fragments arranged by size:
4594 979 923
SanDI GG'GwC_CC
Cuts at: 3778 3778
Size: 6496
SbfI CC_TGCA'GG
Cuts at: 3848 3848
Size: 6496
ScaI AGT'ACT
Cuts at: 4744 6456 4744
Size: 1712 4784
SfcI C'TryA_G
Cuts at: 826 1045 2264 2351 2390 2986 3218 3844 4979
Size: 219 1219 87 39 596 232 626 1135
Cuts at: 4979 5660 5851 826
Size: 681 191 1471
Fragments arranged by size:
1471 1219 1135 681 626 596 232 219
191 87 39
SfoI GGC'GCC
Cuts at: 2019 2512 3102 2019
Size: 493 590 5413
Fragments arranged by size:
5413 590 493
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6496
SmaI CCC'GGG
Cuts at: 1872 3823 1872
Size: 1951 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3073 4600 5501 5763 820
Size: 197 926 1130 1527 901 262 1553
Fragments arranged by size:
1553 1527 1130 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4287 493
Size: 3794 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6496
SphI G_CATG'C
Cuts at: 1132 3854 1132
Size: 2722 3774
SspI AAT'ATT
Cuts at: 5 52 4420 5
Size: 47 4368 2081
Fragments arranged by size:
4368 2081 47
StuI AGG'CCT
Cuts at: 1517 2444 3599 1517
Size: 927 1155 4414
Fragments arranged by size:
4414 1155 927
StyI C'CwwG_G
Cuts at: 513 1065 2004 2583 3237 3309 3532 3935 513
Size: 552 939 579 654 72 223 403 3074
Fragments arranged by size:
3074 939 654 579 552 403 223 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4523 4540 4693 4878 5323 325
Size: 1255 2943 17 153 185 445 1498
Fragments arranged by size:
2943 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3420 3456 4742
Size: 274 80 33 51 1443 1443 36 1286
Cuts at: 4742 6454 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1443 1286 274 138 80 51
36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6456 6488 6456
Size: 32 6464
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6496
XbaI T'CTAG_A
Cuts at: 3832 3832
Size: 6496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3289 3289
Size: 6496
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6496
XmaI C'CCGG_G
Cuts at: 1870 3821 1870
Size: 1951 4545
XmnI GAAnn'nnTTC
Cuts at: 4625 4625
Size: 6496
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4304 276
Size: 53 83 186 936 2770 2468
Fragments arranged by size:
2770 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BfrBI BplI BsiWI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PspXI PsrI RsrII SacI SapI SexAI
SfiI SrfI SwaI