Restriction map for
pFN21AE3253
(Circular) (Six-base) MAPSORT of: pFN21AE3253.seq Check: 9119 from: 1 to: 6472
pFN21AE3253 6472 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2561 2561
Size: 6472
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2626 4282 278
Size: 53 83 186 936 1090 1656 2468
Fragments arranged by size:
2468 1656 1090 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3815 1937
Size: 1878 4594
Acc65I G'GTAC_C
Cuts at: 2485 3793 2485
Size: 1308 5164
AclI AA'CG_TT
Cuts at: 1563 4599 4972 1563
Size: 3036 373 3063
Fragments arranged by size:
3063 3036 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2243 2628 4535 5919 6187 1632
Size: 611 385 1907 1384 268 1917
Fragments arranged by size:
1917 1907 1384 611 385 268
AfeI AGC'GCT
Cuts at: 2432 2432
Size: 6472
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6275
AflIII A'CryG_T
Cuts at: 1203 5371 6348 1203
Size: 4168 977 1327
Fragments arranged by size:
4168 1327 977
AgeI A'CCGG_T
Cuts at: 5303 5303
Size: 6472
AhdI GACnn_n'nnGTC
Cuts at: 5201 5201
Size: 6472
AleI CACnn'nnGTG
Cuts at: 1166 2309 3449 1166
Size: 1143 1140 4189
Fragments arranged by size:
4189 1143 1140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2421 3467 1184
Size: 1237 1046 4189
Fragments arranged by size:
4189 1237 1046
AlwNI CAG_nnn'CTG
Cuts at: 2564 2692 3535 4215 5787 2564
Size: 128 843 680 1572 3249
Fragments arranged by size:
3249 1572 843 680 128
ApaLI G'TGCA_C
Cuts at: 2406 4529 5685 2406
Size: 2123 1156 3193
Fragments arranged by size:
3193 2123 1156
ApoI r'AATT_y
Cuts at: 1493 2027 2852 3781 4007 4159 6268 1493
Size: 534 825 929 226 152 2109 1697
Fragments arranged by size:
2109 1697 929 825 534 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5026 160
Size: 872 3994 1606
Fragments arranged by size:
3994 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
AvaI C'yCGr_G
Cuts at: 1870 1943 2703 3797 1870
Size: 73 760 1094 4545
Fragments arranged by size:
4545 1094 760 73
BamHI G'GATC_C
Cuts at: 3802 4169 3802
Size: 367 6105
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2085 2485 3443 3604 3793
Size: 325 217 627 298 400 958 161 189
Cuts at: 3793 5248 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 958 627 400 325 298 217
189 161
BanII G_rGCy'C
Cuts at: 1391 2233 2632 3791 1391
Size: 842 399 1159 4072
Fragments arranged by size:
4072 1159 842 399
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2269 3495 928
Size: 971 370 1226 3905
Fragments arranged by size:
3905 1226 971 370
BbvCI CC'TCA_GC
Cuts at: 2636 3263 2636
Size: 627 5845
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4663 4697 1903
Size: 34 2726 34 3678
Fragments arranged by size:
3678 2726 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4364 5574 4364
Size: 1210 5262
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6472
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2325 2769 3138
Size: 107 122 71 631 605 653 444 369
Cuts at: 3138 3441 5083 136
Size: 303 1642 1525
Fragments arranged by size:
1642 1525 653 631 605 444 369 303
122 107 71
BglII A'GATC_T
Cuts at: 6467 6467
Size: 6472
BlpI GC'TnA_GC
Cuts at: 3889 3889
Size: 6472
Bme1580I G_kGCm'C
Cuts at: 1790 2316 2410 3098 4533 5689 1790
Size: 526 94 688 1435 1156 2573
Fragments arranged by size:
2573 1435 1156 688 526 94
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6472
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2019 4203 5161
Size: 416 53 83 399 24 602 2184 958
Cuts at: 5161 442
Size: 1753
Fragments arranged by size:
2184 1753 958 602 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6472
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 6472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2960 3512 3683 5132 1239
Size: 1721 552 171 1449 2579
Fragments arranged by size:
2579 1721 1449 552 171
Bpu10I CC'TnA_GC
Cuts at: 2636 3263 2636
Size: 627 5845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3208 4597 5462 5760 3208
Size: 1389 865 298 3920
Fragments arranged by size:
3920 1389 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2065 2741 5135 882
Size: 617 566 676 2394 2219
Fragments arranged by size:
2394 2219 676 617 566
BsaAI yAC'GTr
Cuts at: 493 1204 4263 493
Size: 711 3059 2702
Fragments arranged by size:
3059 2702 711
BsaBI GATnn'nnATC
Cuts at: 6466 6466
Size: 6472
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2535 2623 4279 4661
Size: 53 83 186 936 1002 88 1656 382
Cuts at: 4661 5296 275
Size: 635 1451
Fragments arranged by size:
1656 1451 1002 936 635 382 186 88
83 53
BsaWI w'CCGG_w
Cuts at: 4905 5303 5577 5724 6135 4905
Size: 398 274 147 411 5242
Fragments arranged by size:
5242 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6247
Fragments arranged by size:
6247 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2044 2053 2056 2059 2062 2156 2456 2561
Size: 858 9 3 3 3 94 300 105
Cuts at: 2561 2684 2993 3233 3236 3245 1186
Size: 123 309 240 3 9 4413
Fragments arranged by size:
4413 858 309 300 240 123 105 94
9 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2315 2480 2611 2966 3031 3458 3742 5675 6171
Size: 165 131 355 65 427 284 1933 496
Cuts at: 6171 2315
Size: 2616
Fragments arranged by size:
2616 1933 496 427 355 284 165 131
65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5899
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2665 3922 4683 5711 1540
Size: 275 186 664 1257 761 1028 2301
Fragments arranged by size:
2301 1257 1028 761 664 275 186
BsiHKAI G_wGCw'C
Cuts at: 2410 2632 4533 4618 5689 2410
Size: 222 1901 85 1071 3193
Fragments arranged by size:
3193 1901 1071 222 85
BsmI GAATG_Cn'
Cuts at: 2798 3379 3983 4076 2798
Size: 581 604 93 5194
Fragments arranged by size:
5194 604 581 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 2316 2410 2632 3098 3791 4533
Size: 399 443 83 94 222 466 693 742
Cuts at: 4533 4618 5689 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 693 466 443 399 222
94 85 83
BspEI T'CCGG_A
Cuts at: 6135 6135
Size: 6472
BspHI T'CATG_A
Cuts at: 4361 6091 4361
Size: 1730 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2561 2759 3827 844
Size: 1717 198 1068 3489
Fragments arranged by size:
3489 1717 1068 198
BsrBI CCG'CTC
Cuts at: 1394 1823 4359 1394
Size: 429 2536 3507
Fragments arranged by size:
3507 2536 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4967 5141 65
Size: 1183 3719 174 1396
Fragments arranged by size:
3719 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2577 3766 5116 5303 1700
Size: 110 767 1189 1350 187 2869
Fragments arranged by size:
2869 1350 1189 767 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6472
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6472
BssSI C'ACGA_G
Cuts at: 2414 4532 5544 2414
Size: 2118 1012 3342
Fragments arranged by size:
3342 2118 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2692 2778 794
Size: 1898 86 4488
Fragments arranged by size:
4488 1898 86
BstBI TT'CG_AA
Cuts at: 5276 5276
Size: 6472
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6472
BstYI r'GATC_y
Cuts at: 1972 2733 3490 3706 3802 4169 4554 4571 6023
Size: 761 757 216 96 367 385 17 1452
Cuts at: 6023 6109 6121 6467 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 761 757 385 367 346 216
96 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2320 2838 513
Size: 552 1255 518 4147
Fragments arranged by size:
4147 1255 552 518
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5733
Fragments arranged by size:
5733 683 56
BtsI GCAGTG_nn'
Cuts at: 2468 2690 2722 3988 4782 4802 2468
Size: 222 32 1266 794 20 4138
Fragments arranged by size:
4138 1266 794 222 32 20
ClaI AT'CG_AT
Cuts at: 1554 2661 1554
Size: 1107 5365
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2342 2377 529
Size: 35 1778 35 4624
Fragments arranged by size:
4624 1778 35 35
DraI TTT'AAA
Cuts at: 3776 3776
Size: 6472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2558 5479 809
Size: 507 1242 2921 1802
Fragments arranged by size:
2921 1802 1242 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2575 3006 3315
Size: 54 1425 50 275 18 745 431 309
Cuts at: 3315 3472 3919 4808 6437 8
Size: 157 447 889 1629 43
Fragments arranged by size:
1629 1425 889 745 447 431 309 275
157 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3919 1537
Size: 275 2107 4090
Fragments arranged by size:
4090 2107 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 2080 2119 3222 4402 1353
Size: 662 65 39 1103 1180 3423
Fragments arranged by size:
3423 1180 1103 662 65 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2414 5059 5433 5579 387
Size: 1064 138 825 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 825 374 146 138
EcoICRI GAG'CTC
Cuts at: 2630 2630
Size: 6472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2628 2960 3512 3683 4535 5132
Size: 393 611 385 332 552 171 852 597
Cuts at: 5132 5919 6187 1239
Size: 787 268 1524
Fragments arranged by size:
1524 852 787 611 597 552 393 385
332 268 171
EcoO109I rG'GnC_Cy
Cuts at: 2150 2944 2983 3554 2150
Size: 794 39 571 5068
Fragments arranged by size:
5068 794 571 39
EcoRI G'AATT_C
Cuts at: 3781 4159 3781
Size: 378 6094
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3452 3452
Size: 6472
FspI TGC'GCA
Cuts at: 2391 4978 2391
Size: 2587 3885
HaeII r_GCGC'y
Cuts at: 1678 2245 2434 5619 1678
Size: 567 189 3185 2531
Fragments arranged by size:
3185 2531 567 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2522 2554 5113
Size: 32 135 32 467 32 451 32 2559
Cuts at: 5113 5145 5187 5219 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2559 467 451 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3816 4062 669
Size: 988 281 1878 246 3079
Fragments arranged by size:
3079 1878 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2468 748
Size: 1720 4752
HpaI GTT'AAC
Cuts at: 4062 4062
Size: 6472
KpnI G_GTAC'C
Cuts at: 2489 3797 2489
Size: 1308 5164
MfeI C'AATT_G
Cuts at: 4071 4071
Size: 6472
MluI A'CGCG_T
Cuts at: 6348 6348
Size: 6472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2430 2562 2889 5586 5770 1305
Size: 1125 132 327 2697 184 2007
Fragments arranged by size:
2697 2007 1125 327 184 132
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3008 3317 10
Size: 54 1425 343 1176 309 3165
Fragments arranged by size:
3165 1425 1176 343 309 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2309 3449 4430 4789 4948 518
Size: 648 1143 1140 981 359 159 2042
Fragments arranged by size:
2042 1143 1140 981 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2292 2480 2757 3045 3267 3281 3321
Size: 432 254 188 277 288 222 14 40
Cuts at: 3321 3393 3888 4565 5713 5958 1606
Size: 72 495 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 495 432 288 277 254
245 222 188 72 40 14
NcoI C'CATG_G
Cuts at: 513 1065 2838 513
Size: 552 1773 4147
Fragments arranged by size:
4147 1773 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6472
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6472
NotI GC'GGCC_GC
Cuts at: 3919 3919
Size: 6472
NspI r_CATG'y
Cuts at: 1132 1229 3830 5375 1132
Size: 97 2601 1545 2229
Fragments arranged by size:
2601 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2154 2953 1348
Size: 806 799 4867
Fragments arranged by size:
4867 806 799
PciI A'CATG_T
Cuts at: 5371 5371
Size: 6472
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6472
PfoI T'CCnGG_A
Cuts at: 3363 3660 3363
Size: 297 6175
PmeI GTTT'AAAC
Cuts at: 3776 3776
Size: 6472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4541 4573 6054 6086 4541
Size: 32 1481 32 4927
Fragments arranged by size:
4927 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6472
PsiI TTA'TAA
Cuts at: 4042 4042
Size: 6472
PstI C_TGCA'G
Cuts at: 830 2463 2688 2756 2762 2819 2907 3050 3824
Size: 1633 225 68 6 57 88 143 774
Cuts at: 3824 830
Size: 3478
Fragments arranged by size:
3478 1633 774 225 143 88 68 57
6
PvuI CG_AT'CG
Cuts at: 2001 2665 2001
Size: 664 5808
PvuII CAG'CTG
Cuts at: 2038 2480 2757 3045 3267 3281 3321 3393 2038
Size: 442 277 288 222 14 40 72 5117
Fragments arranged by size:
5117 442 288 277 222 72 40 14
SacI G_AGCT'C
Cuts at: 2632 2632
Size: 6472
SalI G'TCGA_C
Cuts at: 1936 3814 1936
Size: 1878 4594
SapI GCTCTTCn'nnn_
Cuts at: 2119 2119
Size: 6472
SbfI CC_TGCA'GG
Cuts at: 3824 3824
Size: 6472
ScaI AGT'ACT
Cuts at: 4720 6432 4720
Size: 1712 4760
SfcI C'TryA_G
Cuts at: 826 1045 2459 2600 2684 2752 2758 2815 2903
Size: 219 1414 141 84 68 6 57 88
Cuts at: 2903 3046 3343 3820 4955 5636 5827 826
Size: 143 297 477 1135 681 191 1471
Fragments arranged by size:
1471 1414 1135 681 477 297 219 191
143 141 88 84 68 57 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 3138 3138
Size: 6472
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6472
SmaI CCC'GGG
Cuts at: 1872 2705 3799 1872
Size: 833 1094 4545
Fragments arranged by size:
4545 1094 833
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3187 4576 5477 5739 820
Size: 197 926 1244 1389 901 262 1553
Fragments arranged by size:
1553 1389 1244 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4263 493
Size: 3770 2702
SpeI A'CTAG_T
Cuts at: 152 3098 3296 152
Size: 2946 198 3328
Fragments arranged by size:
3328 2946 198
SphI G_CATG'C
Cuts at: 1132 3830 1132
Size: 2698 3774
SspI AAT'ATT
Cuts at: 5 52 4396 5
Size: 47 4344 2081
Fragments arranged by size:
4344 2081 47
StuI AGG'CCT
Cuts at: 1517 3132 1517
Size: 1615 4857
StyI C'CwwG_G
Cuts at: 513 1065 2838 3911 513
Size: 552 1773 1073 3074
Fragments arranged by size:
3074 1773 1073 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2047 2073 2980 3137 3141 3472 4499
Size: 1255 467 26 907 157 4 331 1027
Cuts at: 4499 4516 4669 4854 5299 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1027 907 467 445 331 185
157 153 26 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2330 4718 6430
Size: 274 80 33 51 1443 353 2388 1712
Cuts at: 6430 96
Size: 138
Fragments arranged by size:
2388 1712 1443 353 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3597 3629 6432 6464 3597
Size: 32 2803 32 3605
Fragments arranged by size:
3605 2803 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3737 1316
Size: 2421 4051
XbaI T'CTAG_A
Cuts at: 3808 3808
Size: 6472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2845 3335 2845
Size: 490 5982
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6472
XmaI C'CCGG_G
Cuts at: 1870 2703 3797 1870
Size: 833 1094 4545
Fragments arranged by size:
4545 1094 833
XmnI GAAnn'nnTTC
Cuts at: 4601 4601
Size: 6472
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2624 4280 276
Size: 53 83 186 936 1090 1656 2468
Fragments arranged by size:
2468 1656 1090 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfoI SrfI SwaI