Restriction map for
pFN21AE3252
(Circular) (Six-base) MAPSORT of: pFN21AE3252.seq Check: 2641 from: 1 to: 6469
pFN21AE3252 6469 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2360 2360
Size: 6469
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4279 278
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2412 3812 1937
Size: 475 1400 4594
Fragments arranged by size:
4594 1400 475
Acc65I G'GTAC_C
Cuts at: 3790 3790
Size: 6469
AclI AA'CG_TT
Cuts at: 1563 3768 4596 4969 1563
Size: 2205 828 373 3063
Fragments arranged by size:
3063 2205 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2604 2736 3434 4532 5916 6184 1632
Size: 972 132 698 1098 1384 268 1917
Fragments arranged by size:
1917 1384 1098 972 698 268 132
AflII C'TTAA_G
Cuts at: 820 1017 2665 820
Size: 197 1648 4624
Fragments arranged by size:
4624 1648 197
AflIII A'CryG_T
Cuts at: 1203 5368 6345 1203
Size: 4165 977 1327
Fragments arranged by size:
4165 1327 977
AgeI A'CCGG_T
Cuts at: 5300 5300
Size: 6469
AhdI GACnn_n'nnGTC
Cuts at: 3144 5198 3144
Size: 2054 4415
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2153 1184
Size: 969 5500
AlwNI CAG_nnn'CTG
Cuts at: 2210 3412 4212 5784 2210
Size: 1202 800 1572 2895
Fragments arranged by size:
2895 1572 1202 800
ApaI G_GGCC'C
Cuts at: 3617 3617
Size: 6469
ApaLI G'TGCA_C
Cuts at: 3258 4526 5682 3258
Size: 1268 1156 4045
Fragments arranged by size:
4045 1268 1156
ApoI r'AATT_y
Cuts at: 1493 2184 2400 3489 3778 4004 4156 6265 1493
Size: 691 216 1089 289 226 152 2109 1697
Fragments arranged by size:
2109 1697 1089 691 289 226 216 152
AseI AT'TA_AT
Cuts at: 160 1032 2337 5023 160
Size: 872 1305 2686 1606
Fragments arranged by size:
2686 1606 1305 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
AvaI C'yCGr_G
Cuts at: 1870 1943 3794 1870
Size: 73 1851 4545
Fragments arranged by size:
4545 1851 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2375 2408 2375
Size: 33 6436
BamHI G'GATC_C
Cuts at: 3754 3799 4166 3754
Size: 45 367 6057
Fragments arranged by size:
6057 367 45
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3790 5245 618
Size: 325 217 627 2003 1455 1842
Fragments arranged by size:
2003 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3617 3667 3788 1391
Size: 2226 50 121 4072
Fragments arranged by size:
4072 2226 121 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2184 3033 3114 928
Size: 971 285 849 81 4283
Fragments arranged by size:
4283 971 849 285 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2269 2303 4660 4694 1903
Size: 34 332 34 2357 34 3678
Fragments arranged by size:
3678 2357 332 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2403 4361 5571 2403
Size: 1958 1210 3301
Fragments arranged by size:
3301 1958 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6469
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5080 136
Size: 107 122 71 631 605 3408 1525
Fragments arranged by size:
3408 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6464 6464
Size: 6469
BlpI GC'TnA_GC
Cuts at: 2856 3886 2856
Size: 1030 5439
Bme1580I G_kGCm'C
Cuts at: 1790 3262 3617 4530 5686 1790
Size: 1472 355 913 1156 2573
Fragments arranged by size:
2573 1472 1156 913 355
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6469
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3215 3661 4200
Size: 416 53 83 399 24 1798 446 539
Cuts at: 4200 5158 442
Size: 958 1753
Fragments arranged by size:
1798 1753 958 539 446 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6469
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 6469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3062 5129 1239
Size: 1823 2067 2579
Fragments arranged by size:
2579 2067 1823
Bpu10I CC'TnA_GC
Cuts at: 3474 3474
Size: 6469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2673 4594 5459 5757 2673
Size: 1921 865 298 3385
Fragments arranged by size:
3385 1921 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5132 882
Size: 617 3633 2219
Fragments arranged by size:
3633 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4260 493
Size: 711 3056 2702
Fragments arranged by size:
3056 2702 711
BsaBI GATnn'nnATC
Cuts at: 6463 6463
Size: 6469
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4276 4658 5293 275
Size: 53 83 186 936 2743 382 635 1451
Fragments arranged by size:
2743 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2488 4902 5300 5574 5721 6132 2488
Size: 2414 398 274 147 411 2825
Fragments arranged by size:
2825 2414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6244
Fragments arranged by size:
6244 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2366 1186
Size: 1180 5289
BseYI C'CCAG_C
Cuts at: 5672 6168 5672
Size: 496 5973
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5896
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3919 4680 5708 1540
Size: 275 186 1918 761 1028 2301
Fragments arranged by size:
2301 1918 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2580 3262 4530 4615 5686 2580
Size: 682 1268 85 1071 3363
Fragments arranged by size:
3363 1268 1071 682 85
BsmI GAATG_Cn'
Cuts at: 3589 3980 4073 3589
Size: 391 93 5985
Fragments arranged by size:
5985 391 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3595 3595
Size: 6469
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2580 3262 3617 3667 3788 4530 4615
Size: 399 790 682 355 50 121 742 85
Cuts at: 4615 5686 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 790 742 682 399 355 121
85 50
BspEI T'CCGG_A
Cuts at: 6132 6132
Size: 6469
BspHI T'CATG_A
Cuts at: 2986 3564 4358 6088 2986
Size: 578 794 1730 3367
Fragments arranged by size:
3367 1730 794 578
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2360 3824 844
Size: 1516 1464 3489
Fragments arranged by size:
3489 1516 1464
BsrBI CCG'CTC
Cuts at: 1394 1823 4356 1394
Size: 429 2533 3507
Fragments arranged by size:
3507 2533 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2142 4964 5138 65
Size: 1183 894 2822 174 1396
Fragments arranged by size:
2822 1396 1183 894 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3283 5113 5300 1700
Size: 110 1473 1830 187 2869
Fragments arranged by size:
2869 1830 1473 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6469
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6469
BssSI C'ACGA_G
Cuts at: 4529 5541 4529
Size: 1012 5457
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3266 794
Size: 2472 3997
BstBI TT'CG_AA
Cuts at: 2634 5273 2634
Size: 2639 3830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6469
BstXI CCAn_nnnn'nTGG
Cuts at: 3299 3299
Size: 6469
BstYI r'GATC_y
Cuts at: 1972 3408 3754 3799 4166 4551 4568 6020 6106
Size: 1436 346 45 367 385 17 1452 86
Cuts at: 6106 6118 6464 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1436 385 367 346 346 86
45 17 12
Bsu36I CC'TnA_GG
Cuts at: 2772 3759 2772
Size: 987 5482
BtgI C'CryG_G
Cuts at: 513 1065 2004 3700 513
Size: 552 939 1696 3282
Fragments arranged by size:
3282 1696 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3284 487
Size: 683 56 2058 3672
Fragments arranged by size:
3672 2058 683 56
BtsI GCAGTG_nn'
Cuts at: 3985 4779 4799 3985
Size: 794 20 5655
Fragments arranged by size:
5655 794 20
ClaI AT'CG_AT
Cuts at: 1554 2346 1554
Size: 792 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2042 2077 529
Size: 35 1478 35 4921
Fragments arranged by size:
4921 1478 35 35
DraI TTT'AAA
Cuts at: 2926 3773 2926
Size: 847 5622
DraIII CAC_nnn'GTG
Cuts at: 2447 2447
Size: 6469
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5476 809
Size: 507 4160 1802
Fragments arranged by size:
4160 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3916 4805 6434
Size: 54 1425 50 275 18 2086 889 1629
Cuts at: 6434 8
Size: 43
Fragments arranged by size:
2086 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3916 1537
Size: 275 2104 4090
Fragments arranged by size:
4090 2104 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2631 2868 3357 4399 1353
Size: 1278 237 489 1042 3423
Fragments arranged by size:
3423 1278 1042 489 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5056 5430 5576 387
Size: 1064 138 3467 374 146 1280
Fragments arranged by size:
3467 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2604 2736 3062 3434 4532 5129 5916
Size: 393 972 132 326 372 1098 597 787
Cuts at: 5916 6184 1239
Size: 268 1524
Fragments arranged by size:
1524 1098 972 787 597 393 372 326
268 132
EcoRI G'AATT_C
Cuts at: 3778 4156 3778
Size: 378 6091
FspI TGC'GCA
Cuts at: 4975 4975
Size: 6469
HaeII r_GCGC'y
Cuts at: 1678 5616 1678
Size: 3938 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2964 2996 3130 3162 3364
Size: 32 135 32 1392 32 134 32 202
Cuts at: 3364 3393 3396 3425 5110 5142 5184 5216 1373
Size: 29 3 29 1685 32 42 32 2626
Fragments arranged by size:
2626 1685 1392 202 135 134 42 32
32 32 32 32 32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2599 3289 3813 4059 669
Size: 988 281 661 690 524 246 3079
Fragments arranged by size:
3079 988 690 661 524 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6469
HpaI GTT'AAC
Cuts at: 4059 4059
Size: 6469
KpnI G_GTAC'C
Cuts at: 3794 3794
Size: 6469
MfeI C'AATT_G
Cuts at: 4068 4068
Size: 6469
MluI A'CGCG_T
Cuts at: 6345 6345
Size: 6469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5583 5767 1305
Size: 4278 184 2007
Fragments arranged by size:
4278 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4647
Fragments arranged by size:
4647 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4427 4786 4945 518
Size: 648 3261 359 159 2042
Fragments arranged by size:
3261 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3323 3885 4562 5710 5955 1606
Size: 1717 562 677 1148 245 2120
Fragments arranged by size:
2120 1717 1148 677 562 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3700 513
Size: 552 939 1696 3282
Fragments arranged by size:
3282 1696 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6469
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6469
NotI GC'GGCC_GC
Cuts at: 3916 3916
Size: 6469
NspI r_CATG'y
Cuts at: 1132 1229 3827 5372 1132
Size: 97 2598 1545 2229
Fragments arranged by size:
2598 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6469
PciI A'CATG_T
Cuts at: 5368 5368
Size: 6469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6469
PmeI GTTT'AAAC
Cuts at: 3773 3773
Size: 6469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4538 4570 6051 6083 4538
Size: 32 1481 32 4924
Fragments arranged by size:
4924 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6469
PsiI TTA'TAA
Cuts at: 3522 4039 3522
Size: 517 5952
PspOMI G'GGCC_C
Cuts at: 3613 3613
Size: 6469
PstI C_TGCA'G
Cuts at: 830 3821 830
Size: 2991 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6469
SalI G'TCGA_C
Cuts at: 1936 3811 1936
Size: 1875 4594
SbfI CC_TGCA'GG
Cuts at: 3821 3821
Size: 6469
ScaI AGT'ACT
Cuts at: 4717 6429 4717
Size: 1712 4757
SfcI C'TryA_G
Cuts at: 826 1045 2605 3817 4952 5633 5824 826
Size: 219 1560 1212 1135 681 191 1471
Fragments arranged by size:
1560 1471 1212 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6469
SmaI CCC'GGG
Cuts at: 1872 3796 1872
Size: 1924 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2665 2688 4573 5474 5736 820
Size: 197 926 722 23 1885 901 262 1553
Fragments arranged by size:
1885 1553 926 901 722 262 197 23
SnaBI TAC'GTA
Cuts at: 493 4260 493
Size: 3767 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6469
SphI G_CATG'C
Cuts at: 1132 3827 1132
Size: 2695 3774
SspI AAT'ATT
Cuts at: 5 52 3239 4393 5
Size: 47 3187 1154 2081
Fragments arranged by size:
3187 2081 1154 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6469
StyI C'CwwG_G
Cuts at: 513 1065 2004 3700 3908 513
Size: 552 939 1696 208 3074
Fragments arranged by size:
3074 1696 939 552 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4496 4513 4666 4851 5296 325
Size: 1255 2916 17 153 185 445 1498
Fragments arranged by size:
2916 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4715 6427 96
Size: 274 80 33 51 1443 2738 1712 138
Fragments arranged by size:
2738 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6429 6461 6429
Size: 32 6437
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6469
XbaI T'CTAG_A
Cuts at: 2515 3805 2515
Size: 1290 5179
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2117 2792 2117
Size: 675 5794
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6469
XmaI C'CCGG_G
Cuts at: 1870 3794 1870
Size: 1924 4545
XmnI GAAnn'nnTTC
Cuts at: 2295 2454 3240 3461 4598 2295
Size: 159 786 221 1137 4166
Fragments arranged by size:
4166 1137 786 221 159
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4277 276
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BsiWI
BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI