Restriction map for
pFN21AE3248
(Circular) (Six-base) MAPSORT of: pFN21AE3248.seq Check: 3781 from: 1 to: 6418
pFN21AE3248 6418 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4228 278
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2129 3761 1937
Size: 192 1632 4594
Fragments arranged by size:
4594 1632 192
Acc65I G'GTAC_C
Cuts at: 2193 2928 3739 2193
Size: 735 811 4872
Fragments arranged by size:
4872 811 735
AclI AA'CG_TT
Cuts at: 1563 4545 4918 1563
Size: 2982 373 3063
Fragments arranged by size:
3063 2982 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2748 3347 4481 5865 6133 1632
Size: 1116 599 1134 1384 268 1917
Fragments arranged by size:
1917 1384 1134 1116 599 268
AflII C'TTAA_G
Cuts at: 820 1017 2986 820
Size: 197 1969 4252
Fragments arranged by size:
4252 1969 197
AflIII A'CryG_T
Cuts at: 1203 5317 6294 1203
Size: 4114 977 1327
Fragments arranged by size:
4114 1327 977
AgeI A'CCGG_T
Cuts at: 5249 5249
Size: 6418
AhdI GACnn_n'nnGTC
Cuts at: 5147 5147
Size: 6418
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6418
AlwNI CAG_nnn'CTG
Cuts at: 4161 5733 4161
Size: 1572 4846
ApaLI G'TGCA_C
Cuts at: 4475 5631 4475
Size: 1156 5262
ApoI r'AATT_y
Cuts at: 1493 2209 2602 3616 3727 3953 4105 6214 1493
Size: 716 393 1014 111 226 152 2109 1697
Fragments arranged by size:
2109 1697 1014 716 393 226 152 111
AseI AT'TA_AT
Cuts at: 160 1032 4972 160
Size: 872 3940 1606
Fragments arranged by size:
3940 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
AvaI C'yCGr_G
Cuts at: 1870 1943 3743 1870
Size: 73 1800 4545
Fragments arranged by size:
4545 1800 73
BamHI G'GATC_C
Cuts at: 3202 3410 3748 4115 3202
Size: 208 338 367 5505
Fragments arranged by size:
5505 367 338 208
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2193 2918 2928 3391 3739
Size: 325 217 627 406 725 10 463 348
Cuts at: 3739 5194 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 725 627 463 406 348 325
217 10
BanII G_rGCy'C
Cuts at: 1391 2082 3737 1391
Size: 691 1655 4072
Fragments arranged by size:
4072 1655 691
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5447
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4609 4643 1903
Size: 34 2672 34 3678
Fragments arranged by size:
3678 2672 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3652 4310 5520 3652
Size: 658 1210 4550
Fragments arranged by size:
4550 1210 658
BclI T'GATC_A
Cuts at: 1548 3144 1548
Size: 1596 4822
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3400 5029 136
Size: 107 122 71 631 605 1728 1629 1525
Fragments arranged by size:
1728 1629 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6413 6413
Size: 6418
BlpI GC'TnA_GC
Cuts at: 2549 3835 2549
Size: 1286 5132
Bme1580I G_kGCm'C
Cuts at: 1790 4479 5635 1790
Size: 2689 1156 2573
Fragments arranged by size:
2689 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6418
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4149 5107 442
Size: 416 53 83 399 24 2732 958 1753
Fragments arranged by size:
2732 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6418
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3559 3559
Size: 6418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2691 3435 5078 1239
Size: 1452 744 1643 2579
Fragments arranged by size:
2579 1643 1452 744
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3529 4543 5408 5706 3529
Size: 1014 865 298 4241
Fragments arranged by size:
4241 1014 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5081 882
Size: 617 3582 2219
Fragments arranged by size:
3582 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4209 493
Size: 711 3005 2702
Fragments arranged by size:
3005 2702 711
BsaBI GATnn'nnATC
Cuts at: 2313 3423 6412 2313
Size: 1110 2989 2319
Fragments arranged by size:
2989 2319 1110
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 4225 4607 5242
Size: 53 83 186 936 489 2203 382 635
Cuts at: 5242 275
Size: 1451
Fragments arranged by size:
2203 1451 936 635 489 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4851 5249 5523 5670 6081 4851
Size: 398 274 147 411 5188
Fragments arranged by size:
5188 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2821 2851 1178
Size: 30 165 30 1418 30 4745
Fragments arranged by size:
4745 1418 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2474 1186
Size: 1288 5130
BseYI C'CCAG_C
Cuts at: 5621 6117 5621
Size: 496 5922
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2892 1163
Size: 573 1156 4689
Fragments arranged by size:
4689 1156 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2133 3868 4629 5657 1540
Size: 275 186 132 1735 761 1028 2301
Fragments arranged by size:
2301 1735 1028 761 275 186 132
BsiHKAI G_wGCw'C
Cuts at: 3655 4479 4564 5635 3655
Size: 824 85 1071 4438
Fragments arranged by size:
4438 1071 824 85
BsmI GAATG_Cn'
Cuts at: 3929 4022 3929
Size: 93 6325
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 3655 3737 4479 4564 5635 1391
Size: 399 292 1573 82 742 85 1071 2174
Fragments arranged by size:
2174 1573 1071 742 399 292 85 82
BspEI T'CCGG_A
Cuts at: 6081 6081
Size: 6418
BspHI T'CATG_A
Cuts at: 4307 6037 4307
Size: 1730 4688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3773 844
Size: 2929 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4305 1394
Size: 429 2482 3507
Fragments arranged by size:
3507 2482 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4913 5087 65
Size: 1183 3665 174 1396
Fragments arranged by size:
3665 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3399 5062 5249 1700
Size: 110 1589 1663 187 2869
Fragments arranged by size:
2869 1663 1589 187 110
BsrGI T'GTAC_A
Cuts at: 96 2734 96
Size: 2638 3780
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6418
BssSI C'ACGA_G
Cuts at: 2072 2993 4478 5490 2072
Size: 921 1485 1012 3000
Fragments arranged by size:
3000 1485 1012 921
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3391 794
Size: 2597 3821
BstBI TT'CG_AA
Cuts at: 5222 5222
Size: 6418
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6418
BstYI r'GATC_y
Cuts at: 1972 2055 3202 3410 3748 4115 4500 4517 5969
Size: 83 1147 208 338 367 385 17 1452
Cuts at: 5969 6055 6067 6413 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1147 385 367 346 338 208
86 83 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2900 513
Size: 552 939 896 4031
Fragments arranged by size:
4031 939 896 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2519 487
Size: 683 56 1293 4386
Fragments arranged by size:
4386 1293 683 56
BtsI GCAGTG_nn'
Cuts at: 3934 4728 4748 3934
Size: 794 20 5604
Fragments arranged by size:
5604 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6418
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6383
DraI TTT'AAA
Cuts at: 3573 3722 3573
Size: 149 6269
DraIII CAC_nnn'GTG
Cuts at: 2109 2109
Size: 6418
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5425 809
Size: 507 4109 1802
Fragments arranged by size:
4109 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2284 3865 4754
Size: 54 1425 50 275 18 454 1581 889
Cuts at: 4754 6383 8
Size: 1629 43
Fragments arranged by size:
1629 1581 1425 889 454 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3865 1537
Size: 275 2053 4090
Fragments arranged by size:
4090 2053 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2350 2361 2463 2494 3123 4348 1353
Size: 997 11 102 31 629 1225 3423
Fragments arranged by size:
3423 1225 997 629 102 31 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5005 5379 5525 387
Size: 1064 138 3416 374 146 1280
Fragments arranged by size:
3416 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2691 2748 3347 3435 4481 5078 5865
Size: 393 1059 57 599 88 1046 597 787
Cuts at: 5865 6133 1239
Size: 268 1524
Fragments arranged by size:
1524 1059 1046 787 599 597 393 268
88 57
EcoRI G'AATT_C
Cuts at: 2602 3727 4105 2602
Size: 1125 378 4915
Fragments arranged by size:
4915 1125 378
EcoRV GAT'ATC
Cuts at: 2847 2847
Size: 6418
FspI TGC'GCA
Cuts at: 4924 4924
Size: 6418
HaeII r_GCGC'y
Cuts at: 1678 5565 1678
Size: 3887 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2744 2776 2821 2853 3466
Size: 32 135 32 1172 32 45 32 613
Cuts at: 3466 3498 5059 5091 5133 5165 1373
Size: 32 1561 32 42 32 2626
Fragments arranged by size:
2626 1561 1172 613 135 45 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2130 2221 3762 4008 669
Size: 988 281 192 91 1541 246 3079
Fragments arranged by size:
3079 1541 988 281 246 192 91
HindIII A'AGCT_T
Cuts at: 748 2270 748
Size: 1522 4896
HpaI GTT'AAC
Cuts at: 4008 4008
Size: 6418
KpnI G_GTAC'C
Cuts at: 2197 2932 3743 2197
Size: 735 811 4872
Fragments arranged by size:
4872 811 735
MfeI C'AATT_G
Cuts at: 4017 4017
Size: 6418
MluI A'CGCG_T
Cuts at: 6294 6294
Size: 6418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2839 3551 5532 5716 1305
Size: 1534 712 1981 184 2007
Fragments arranged by size:
2007 1981 1534 712 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2286 10
Size: 54 1425 343 454 4142
Fragments arranged by size:
4142 1425 454 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2905 4376 4735 4894 518
Size: 648 1739 1471 359 159 2042
Fragments arranged by size:
2042 1739 1471 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3834 4511 5659 5904 1606
Size: 2228 677 1148 245 2120
Fragments arranged by size:
2228 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2900 513
Size: 552 939 896 4031
Fragments arranged by size:
4031 939 896 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6418
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6418
NotI GC'GGCC_GC
Cuts at: 3865 3865
Size: 6418
NspI r_CATG'y
Cuts at: 1132 1229 3776 5321 1132
Size: 97 2547 1545 2229
Fragments arranged by size:
2547 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2259 1348
Size: 911 5507
PciI A'CATG_T
Cuts at: 5317 5317
Size: 6418
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6418
PmeI GTTT'AAAC
Cuts at: 3722 3722
Size: 6418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4487 4519 6000 6032 4487
Size: 32 1481 32 4873
Fragments arranged by size:
4873 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6418
PsiI TTA'TAA
Cuts at: 3988 3988
Size: 6418
PstI C_TGCA'G
Cuts at: 830 3037 3770 830
Size: 2207 733 3478
Fragments arranged by size:
3478 2207 733
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6418
SalI G'TCGA_C
Cuts at: 1936 2128 3760 1936
Size: 192 1632 4594
Fragments arranged by size:
4594 1632 192
SapI GCTCTTCn'nnn_
Cuts at: 2494 2494
Size: 6418
SbfI CC_TGCA'GG
Cuts at: 3770 3770
Size: 6418
ScaI AGT'ACT
Cuts at: 4666 6378 4666
Size: 1712 4706
SfcI C'TryA_G
Cuts at: 826 1045 3033 3662 3766 4901 5582 5773 826
Size: 219 1988 629 104 1135 681 191 1471
Fragments arranged by size:
1988 1471 1135 681 629 219 191 104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6418
SmaI CCC'GGG
Cuts at: 1872 3745 1872
Size: 1873 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2986 3544 4522 5423 5685 820
Size: 197 926 1043 558 978 901 262 1553
Fragments arranged by size:
1553 1043 978 926 901 558 262 197
SnaBI TAC'GTA
Cuts at: 493 4209 493
Size: 3716 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6418
SphI G_CATG'C
Cuts at: 1132 3776 1132
Size: 2644 3774
SspI AAT'ATT
Cuts at: 5 52 4342 5
Size: 47 4290 2081
Fragments arranged by size:
4290 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6418
StyI C'CwwG_G
Cuts at: 513 1065 2004 2851 2900 3857 513
Size: 552 939 847 49 957 3074
Fragments arranged by size:
3074 957 939 847 552 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2644 3054 4445 4462 4615 4800 5245
Size: 1255 1064 410 1391 17 153 185 445
Cuts at: 5245 325
Size: 1498
Fragments arranged by size:
1498 1391 1255 1064 445 410 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2734 4664 6376
Size: 274 80 33 51 1443 757 1930 1712
Cuts at: 6376 96
Size: 138
Fragments arranged by size:
1930 1712 1443 757 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3508 3540 6378 6410 3508
Size: 32 2838 32 3516
Fragments arranged by size:
3516 2838 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6418
XbaI T'CTAG_A
Cuts at: 3754 3754
Size: 6418
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6418
XmaI C'CCGG_G
Cuts at: 1870 3743 1870
Size: 1873 4545
XmnI GAAnn'nnTTC
Cuts at: 2209 4547 2209
Size: 2338 4080
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4226 276
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI