Restriction map for
pFN21AE3245
(Circular) (Six-base) MAPSORT of: pFN21AE3245.seq Check: 8249 from: 1 to: 6409
pFN21AE3245 6409 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4219 278
Size: 53 83 186 936 2683 2468
Fragments arranged by size:
2683 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2321 2586 3752 1937
Size: 384 265 1166 4594
Fragments arranged by size:
4594 1166 384 265
Acc65I G'GTAC_C
Cuts at: 3730 3730
Size: 6409
AclI AA'CG_TT
Cuts at: 1563 4536 4909 1563
Size: 2973 373 3063
Fragments arranged by size:
3063 2973 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3558 4472 5856 6124 1632
Size: 1926 914 1384 268 1917
Fragments arranged by size:
1926 1917 1384 914 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6212
AflIII A'CryG_T
Cuts at: 1203 3027 3661 5308 6285 1203
Size: 1824 634 1647 977 1327
Fragments arranged by size:
1824 1647 1327 977 634
AgeI A'CCGG_T
Cuts at: 5240 5240
Size: 6409
AhdI GACnn_n'nnGTC
Cuts at: 5138 5138
Size: 6409
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3138 1184
Size: 1954 4455
AlwNI CAG_nnn'CTG
Cuts at: 3536 4152 5724 3536
Size: 616 1572 4221
Fragments arranged by size:
4221 1572 616
ApaLI G'TGCA_C
Cuts at: 4466 5622 4466
Size: 1156 5253
ApoI r'AATT_y
Cuts at: 1493 2348 2628 2684 2984 3152 3651 3718 3944
Size: 855 280 56 300 168 499 67 226
Cuts at: 3944 4096 6205 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 855 499 300 280 226 168
152 67 56
AseI AT'TA_AT
Cuts at: 160 1032 2364 4963 160
Size: 872 1332 2599 1606
Fragments arranged by size:
2599 1606 1332 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6409
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2904 3734 1870
Size: 73 99 862 830 4545
Fragments arranged by size:
4545 862 830 99 73
AvrII C'CTAG_G
Cuts at: 2720 2720
Size: 6409
BamHI G'GATC_C
Cuts at: 2491 3739 4106 2491
Size: 1248 367 4794
Fragments arranged by size:
4794 1248 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3730 5185 618
Size: 325 217 627 1943 1455 1842
Fragments arranged by size:
1943 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3147 3728 1391
Size: 1756 581 4072
Fragments arranged by size:
4072 1756 581
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2099 2133 3689 928
Size: 971 200 34 1556 3648
Fragments arranged by size:
3648 1556 971 200 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4600 4634 1903
Size: 34 2663 34 3678
Fragments arranged by size:
3678 2663 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4301 5511 4301
Size: 1210 5199
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6409
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5020 136
Size: 107 122 71 631 605 3348 1525
Fragments arranged by size:
3348 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2974 6404 2974
Size: 3430 2979
BlpI GC'TnA_GC
Cuts at: 3826 3826
Size: 6409
Bme1580I G_kGCm'C
Cuts at: 1790 4470 5626 1790
Size: 2680 1156 2573
Fragments arranged by size:
2680 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3028 1318
Size: 1710 4699
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2221 2227 4140
Size: 416 53 83 399 24 804 6 1913
Cuts at: 4140 5098 442
Size: 958 1753
Fragments arranged by size:
1913 1753 958 804 416 399 83 53
24 6
BmtI G_CTAG'C
Cuts at: 1056 2598 1056
Size: 1542 4867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2310 5069 1239
Size: 1071 2759 2579
Fragments arranged by size:
2759 2579 1071
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3094 4534 5399 5697 3094
Size: 1440 865 298 3806
Fragments arranged by size:
3806 1440 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3543 5072 882
Size: 617 2044 1529 2219
Fragments arranged by size:
2219 2044 1529 617
BsaAI yAC'GTr
Cuts at: 493 1204 4200 493
Size: 711 2996 2702
Fragments arranged by size:
2996 2702 711
BsaBI GATnn'nnATC
Cuts at: 2442 3195 6403 2442
Size: 753 3208 2448
Fragments arranged by size:
3208 2448 753
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4216 4598 5233 275
Size: 53 83 186 936 2683 382 635 1451
Fragments arranged by size:
2683 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4842 5240 5514 5661 6072 4842
Size: 398 274 147 411 5179
Fragments arranged by size:
5179 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2048 2078 1178
Size: 30 165 30 645 30 5509
Fragments arranged by size:
5509 645 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2056 2192 2195 2198 1186
Size: 870 136 3 3 5397
Fragments arranged by size:
5397 870 136 3 3
BseYI C'CCAG_C
Cuts at: 5612 6108 5612
Size: 496 5913
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2847 3438 1163
Size: 573 1111 591 4134
Fragments arranged by size:
4134 1111 591 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3859 4620 5648 1540
Size: 275 186 1858 761 1028 2301
Fragments arranged by size:
2301 1858 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3048 3147 3524 4470 4555 5626 3048
Size: 99 377 946 85 1071 3831
Fragments arranged by size:
3831 1071 946 377 99 85
BsmI GAATG_Cn'
Cuts at: 3382 3920 4013 3382
Size: 538 93 5778
Fragments arranged by size:
5778 538 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3048 3147 3524 3728 4470 4555 5626
Size: 399 1258 99 377 204 742 85 1071
Cuts at: 5626 1391
Size: 2174
Fragments arranged by size:
2174 1258 1071 742 399 377 204 99
85
BspEI T'CCGG_A
Cuts at: 6072 6072
Size: 6409
BspHI T'CATG_A
Cuts at: 2025 2832 3129 4298 6028 2025
Size: 807 297 1169 1730 2406
Fragments arranged by size:
2406 1730 1169 807 297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2911 2925 3764 844
Size: 2067 14 839 3489
Fragments arranged by size:
3489 2067 839 14
BsrBI CCG'CTC
Cuts at: 1394 1823 4296 1394
Size: 429 2473 3507
Fragments arranged by size:
3507 2473 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4904 5078 65
Size: 1183 3656 174 1396
Fragments arranged by size:
3656 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2013 5053 5240 1700
Size: 110 203 3040 187 2869
Fragments arranged by size:
3040 2869 203 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6409
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6409
BssSI C'ACGA_G
Cuts at: 4469 5481 4469
Size: 1012 5397
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6409
BstBI TT'CG_AA
Cuts at: 5213 5213
Size: 6409
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6409
BstYI r'GATC_y
Cuts at: 1972 2491 2974 3163 3739 4106 4491 4508 5960
Size: 519 483 189 576 367 385 17 1452
Cuts at: 5960 6046 6058 6404 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 576 519 483 385 367 346
189 86 17 12
BstZ17I GTA'TAC
Cuts at: 2587 2587
Size: 6409
BtgI C'CryG_G
Cuts at: 513 1065 2004 3001 3039 3210 3576 513
Size: 552 939 997 38 171 366 3346
Fragments arranged by size:
3346 997 939 552 366 171 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5670
Fragments arranged by size:
5670 683 56
BtsI GCAGTG_nn'
Cuts at: 3925 4719 4739 3925
Size: 794 20 5595
Fragments arranged by size:
5595 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6409
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3045 3080 529
Size: 35 2481 35 3858
Fragments arranged by size:
3858 2481 35 35
DraI TTT'AAA
Cuts at: 3713 3713
Size: 6409
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5416 809
Size: 507 4100 1802
Fragments arranged by size:
4100 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3369 3856 4745
Size: 54 1425 50 275 18 1539 487 889
Cuts at: 4745 6374 8
Size: 1629 43
Fragments arranged by size:
1629 1539 1425 889 487 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3856 1537
Size: 275 2044 4090
Fragments arranged by size:
4090 2044 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3264 3354 3534 4339 1353
Size: 1911 90 180 805 3423
Fragments arranged by size:
3423 1911 805 180 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4996 5370 5516 387
Size: 1064 138 3407 374 146 1280
Fragments arranged by size:
3407 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3145 3145
Size: 6409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2310 3558 4472 5069 5856 6124 1239
Size: 393 678 1248 914 597 787 268 1524
Fragments arranged by size:
1524 1248 914 787 678 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3448 3448
Size: 6409
EcoRI G'AATT_C
Cuts at: 2684 3718 4096 2684
Size: 1034 378 4997
Fragments arranged by size:
4997 1034 378
FspI TGC'GCA
Cuts at: 4915 4915
Size: 6409
HaeII r_GCGC'y
Cuts at: 1678 5556 1678
Size: 3878 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2779 2811 3518
Size: 32 135 32 476 32 699 32 707
Cuts at: 3518 3550 5050 5082 5124 5156 1373
Size: 32 1500 32 42 32 2626
Fragments arranged by size:
2626 1500 707 699 476 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2526 3753 3999 669
Size: 988 281 588 1227 246 3079
Fragments arranged by size:
3079 1227 988 588 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6409
HpaI GTT'AAC
Cuts at: 3999 3999
Size: 6409
KpnI G_GTAC'C
Cuts at: 3734 3734
Size: 6409
MfeI C'AATT_G
Cuts at: 4008 4008
Size: 6409
MluI A'CGCG_T
Cuts at: 6285 6285
Size: 6409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2212 2322 2469 5523 5707 1305
Size: 907 110 147 3054 184 2007
Fragments arranged by size:
3054 2007 907 184 147 110
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3371 10
Size: 54 1425 343 1539 3048
Fragments arranged by size:
3048 1539 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3026 3057 3134 3264 3344 3371 4367
Size: 648 1860 31 77 130 80 27 996
Cuts at: 4367 4726 4885 518
Size: 359 159 2042
Fragments arranged by size:
2042 1860 996 648 359 159 130 80
77 31 27
MspA1I CmG'CkG
Cuts at: 1606 3825 4502 5650 5895 1606
Size: 2219 677 1148 245 2120
Fragments arranged by size:
2219 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3210 513
Size: 552 939 1206 3712
Fragments arranged by size:
3712 1206 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6409
NheI G'CTAG_C
Cuts at: 1052 2594 1052
Size: 1542 4867
NotI GC'GGCC_GC
Cuts at: 3856 3856
Size: 6409
NspI r_CATG'y
Cuts at: 1132 1229 2860 2995 3062 3665 3767 5312 1132
Size: 97 1631 135 67 603 102 1545 2229
Fragments arranged by size:
2229 1631 1545 603 135 102 97 67
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6409
PciI A'CATG_T
Cuts at: 3661 5308 3661
Size: 1647 4762
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6409
PmeI GTTT'AAAC
Cuts at: 3713 3713
Size: 6409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4478 4510 5991 6023 4478
Size: 32 1481 32 4864
Fragments arranged by size:
4864 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3448 3448
Size: 6409
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6409
PsiI TTA'TAA
Cuts at: 2634 3979 2634
Size: 1345 5064
PstI C_TGCA'G
Cuts at: 830 2922 3761 830
Size: 2092 839 3478
Fragments arranged by size:
3478 2092 839
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6409
SacI G_AGCT'C
Cuts at: 3147 3147
Size: 6409
SalI G'TCGA_C
Cuts at: 1936 3751 1936
Size: 1815 4594
SapI GCTCTTCn'nnn_
Cuts at: 3534 3534
Size: 6409
SbfI CC_TGCA'GG
Cuts at: 2922 3761 2922
Size: 839 5570
ScaI AGT'ACT
Cuts at: 4657 6369 4657
Size: 1712 4697
SfcI C'TryA_G
Cuts at: 826 1045 2475 2918 3757 4892 5573 5764 826
Size: 219 1430 443 839 1135 681 191 1471
Fragments arranged by size:
1471 1430 1135 839 681 443 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6409
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6409
SmaI CCC'GGG
Cuts at: 1872 2044 3736 1872
Size: 172 1692 4545
Fragments arranged by size:
4545 1692 172
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3073 4513 5414 5676 820
Size: 197 926 1130 1440 901 262 1553
Fragments arranged by size:
1553 1440 1130 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4200 493
Size: 3707 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6409
SphI G_CATG'C
Cuts at: 1132 3767 1132
Size: 2635 3774
SspI AAT'ATT
Cuts at: 5 52 3394 4333 5
Size: 47 3342 939 2081
Fragments arranged by size:
3342 2081 939 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6409
StyI C'CwwG_G
Cuts at: 513 1065 2004 2244 2720 2898 3063 3210 3234
Size: 552 939 240 476 178 165 147 24
Cuts at: 3234 3848 513
Size: 614 3074
Fragments arranged by size:
3074 939 614 552 476 240 178 165
147 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3069 4436 4453 4606 4791 5236 325
Size: 1255 1489 1367 17 153 185 445 1498
Fragments arranged by size:
1498 1489 1367 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2931 4655 6367
Size: 274 80 33 51 1443 954 1724 1712
Cuts at: 6367 96
Size: 138
Fragments arranged by size:
1724 1712 1443 954 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6369 6401 6369
Size: 32 6377
Tth111I GACn'n_nGTC
Cuts at: 1316 2336 1316
Size: 1020 5389
XbaI T'CTAG_A
Cuts at: 2420 3745 2420
Size: 1325 5084
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6409
XmaI C'CCGG_G
Cuts at: 1870 2042 3734 1870
Size: 172 1692 4545
Fragments arranged by size:
4545 1692 172
XmnI GAAnn'nnTTC
Cuts at: 2130 4538 2130
Size: 2408 4001
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4217 276
Size: 53 83 186 936 2683 2468
Fragments arranged by size:
2683 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI