Restriction map for
pFN21AE3244
(Circular) (Six-base) MAPSORT of: pFN21AE3244.seq Check: 7161 from: 1 to: 6406
pFN21AE3244 6406 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3550 3550
Size: 6406
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3749 1937
Size: 1812 4594
Acc65I G'GTAC_C
Cuts at: 3727 3727
Size: 6406
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3723 4469 5853 6121 1632
Size: 2091 746 1384 268 1917
Fragments arranged by size:
2091 1917 1384 746 268
AfeI AGC'GCT
Cuts at: 2318 2318
Size: 6406
AflII C'TTAA_G
Cuts at: 820 1017 3088 820
Size: 197 2071 4138
Fragments arranged by size:
4138 2071 197
AflIII A'CryG_T
Cuts at: 1203 5305 6282 1203
Size: 4102 977 1327
Fragments arranged by size:
4102 1327 977
AgeI A'CCGG_T
Cuts at: 5237 5237
Size: 6406
AhdI GACnn_n'nnGTC
Cuts at: 5135 5135
Size: 6406
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6406
AlwNI CAG_nnn'CTG
Cuts at: 2415 4149 5721 2415
Size: 1734 1572 3100
Fragments arranged by size:
3100 1734 1572
ApaI G_GGCC'C
Cuts at: 3583 3591 3583
Size: 8 6398
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 2546 2720 2991 3104 3204 3715 3941 4093
Size: 1053 174 271 113 100 511 226 152
Cuts at: 4093 6202 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1053 511 271 226 174 152
113 100
AseI AT'TA_AT
Cuts at: 160 1032 2589 2663 4960 160
Size: 872 1557 74 2297 1606
Fragments arranged by size:
2297 1606 1557 872 74
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 2820 3731 1870
Size: 73 877 911 4545
Fragments arranged by size:
4545 911 877 73
BamHI G'GATC_C
Cuts at: 2385 3736 4103 2385
Size: 1351 367 4688
Fragments arranged by size:
4688 1351 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2064 2255 2478 3181
Size: 325 217 627 239 38 191 223 703
Cuts at: 3181 3727 5182 618
Size: 546 1455 1842
Fragments arranged by size:
1842 1455 703 627 546 325 239 223
217 191 38
BanII G_rGCy'C
Cuts at: 1391 2036 2280 3583 3591 3725 1391
Size: 645 244 1303 8 134 4072
Fragments arranged by size:
4072 1303 645 244 134 8
BbeI G_GCGC'C
Cuts at: 2030 2259 2030
Size: 229 6177
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5435
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4597 4631 1903
Size: 34 2660 34 3678
Fragments arranged by size:
3678 2660 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3071 3347 4298 5508 3071
Size: 276 951 1210 3969
Fragments arranged by size:
3969 1210 951 276
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5017 136
Size: 107 122 71 631 605 3345 1525
Fragments arranged by size:
3345 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6401 6401
Size: 6406
BlpI GC'TnA_GC
Cuts at: 3823 3823
Size: 6406
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2403 3583 3591 4467 5623 1790
Size: 277 336 1180 8 876 1156 2573
Fragments arranged by size:
2573 1180 1156 876 336 277 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3367 4137 5095
Size: 416 53 83 399 24 1950 770 958
Cuts at: 5095 442
Size: 1753
Fragments arranged by size:
1950 1753 958 770 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2633 1056
Size: 1577 4829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 3006 3663 5066 1239
Size: 875 892 657 1403 2579
Fragments arranged by size:
2579 1403 892 875 657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2327 4531 5396 5694 2327
Size: 2204 865 298 3039
Fragments arranged by size:
3039 2204 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5069 882
Size: 617 3570 2219
Fragments arranged by size:
3570 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4197 493
Size: 711 2993 2702
Fragments arranged by size:
2993 2702 711
BsaBI GATnn'nnATC
Cuts at: 6400 6400
Size: 6406
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 2256 2367 4213
Size: 53 83 186 936 494 229 111 1846
Cuts at: 4213 4595 5230 275
Size: 382 635 1451
Fragments arranged by size:
1846 1451 936 635 494 382 229 186
111 83 53
BsaWI w'CCGG_w
Cuts at: 4839 5237 5511 5658 6069 4839
Size: 398 274 147 411 5176
Fragments arranged by size:
5176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2752 2782 1178
Size: 30 165 30 1349 30 4802
Fragments arranged by size:
4802 1349 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2760 1186
Size: 1574 4832
BseYI C'CCAG_C
Cuts at: 3320 5609 6105 3320
Size: 2289 496 3621
Fragments arranged by size:
3621 2289 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 2428 3577 1163
Size: 573 399 293 1149 3992
Fragments arranged by size:
3992 1149 573 399 293
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2024 2138 3856 4617 5645 1540
Size: 275 186 23 114 1718 761 1028 2301
Fragments arranged by size:
2301 1718 1028 761 275 186 114 23
BsiHKAI G_wGCw'C
Cuts at: 2090 3472 3545 4467 4552 5623 2090
Size: 1382 73 922 85 1071 2873
Fragments arranged by size:
2873 1382 1071 922 85 73
BsmI GAATG_Cn'
Cuts at: 3917 4010 3917
Size: 93 6313
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2067 2090 2280 2403 3472 3545
Size: 399 246 31 23 190 123 1069 73
Cuts at: 3545 3583 3591 3725 4467 4552 5623 1391
Size: 38 8 134 742 85 1071 2174
Fragments arranged by size:
2174 1071 1069 742 399 246 190 134
123 85 73 38 31 23 8
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 4295 6025 4295
Size: 1730 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2995 3550 3761 844
Size: 2151 555 211 3489
Fragments arranged by size:
3489 2151 555 211
BsrBI CCG'CTC
Cuts at: 1394 1823 4293 1394
Size: 429 2470 3507
Fragments arranged by size:
3507 2470 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3630 4901 5075 65
Size: 1183 2382 1271 174 1396
Fragments arranged by size:
2382 1396 1271 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2023 2101 2142 5050 5237 1700
Size: 110 213 78 41 2908 187 2869
Fragments arranged by size:
2908 2869 213 187 110 78 41
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6406
BssHII G'CGCG_C
Cuts at: 1925 2235 1925
Size: 310 6096
BssSI C'ACGA_G
Cuts at: 4466 5478 4466
Size: 1012 5394
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2248 2478 3010 794
Size: 1454 230 532 4190
Fragments arranged by size:
4190 1454 532 230
BstBI TT'CG_AA
Cuts at: 3502 5210 3502
Size: 1708 4698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6406
BstXI CCAn_nnnn'nTGG
Cuts at: 2362 2962 2362
Size: 600 5806
BstYI r'GATC_y
Cuts at: 1972 2385 2727 3736 4103 4488 4505 5957 6043
Size: 413 342 1009 367 385 17 1452 86
Cuts at: 6043 6055 6401 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1009 413 385 367 346 342
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2300 2300
Size: 6406
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4915
Fragments arranged by size:
4915 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5667
Fragments arranged by size:
5667 683 56
BtsI GCAGTG_nn'
Cuts at: 2441 2657 3314 3922 4716 4736 2441
Size: 216 657 608 794 20 4111
Fragments arranged by size:
4111 794 657 608 216 20
ClaI AT'CG_AT
Cuts at: 1554 3049 1554
Size: 1495 4911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3543 3578 529
Size: 35 2979 35 3357
Fragments arranged by size:
3357 2979 35 35
DraI TTT'AAA
Cuts at: 2520 3710 2520
Size: 1190 5216
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2357 5413 809
Size: 507 1041 3056 1802
Fragments arranged by size:
3056 1802 1041 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2021 2673 3853
Size: 54 1425 50 275 18 191 652 1180
Cuts at: 3853 4742 6371 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1180 889 652 275 191 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2021 3853 1537
Size: 275 209 1832 4090
Fragments arranged by size:
4090 1832 275 209
EarI CTCTTCn'nnn_
Cuts at: 1353 2805 4336 1353
Size: 1452 1531 3423
Fragments arranged by size:
3423 1531 1452
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4993 5367 5513 387
Size: 1064 138 3404 374 146 1280
Fragments arranged by size:
3404 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 3006 3663 3723 4469 5066 5853
Size: 393 482 892 657 60 746 597 787
Cuts at: 5853 6121 1239
Size: 268 1524
Fragments arranged by size:
1524 892 787 746 657 597 482 393
268 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2303 2303
Size: 6406
EcoO109I rG'GnC_Cy
Cuts at: 2047 2359 2860 3587 3588 2047
Size: 312 501 727 1 4865
Fragments arranged by size:
4865 727 501 312 1
EcoRI G'AATT_C
Cuts at: 2546 3104 3204 3715 4093 2546
Size: 558 100 511 378 4859
Fragments arranged by size:
4859 558 511 378 100
FspI TGC'GCA
Cuts at: 3003 4912 3003
Size: 1909 4497
HaeII r_GCGC'y
Cuts at: 1678 2030 2259 2320 5553 1678
Size: 352 229 61 3233 2531
Fragments arranged by size:
3233 2531 352 229 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3391 3423 3650 3682 5047
Size: 32 135 32 1819 32 227 32 1365
Cuts at: 5047 5079 5121 5153 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1819 1365 227 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2447 3750 3996 669
Size: 988 281 509 1303 246 3079
Fragments arranged by size:
3079 1303 988 509 281 246
HindIII A'AGCT_T
Cuts at: 748 2453 2639 748
Size: 1705 186 4515
Fragments arranged by size:
4515 1705 186
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KasI G'GCGC_C
Cuts at: 2026 2255 2026
Size: 229 6177
KpnI G_GTAC'C
Cuts at: 3731 3731
Size: 6406
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3112 3424 5520 5704 1305
Size: 1807 312 2096 184 2007
Fragments arranged by size:
2096 2007 1807 312 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2675 10
Size: 54 1425 343 843 3741
Fragments arranged by size:
3741 1425 843 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3423 4364 4723 4882 518
Size: 648 2257 941 359 159 2042
Fragments arranged by size:
2257 2042 941 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2134 2149 3822 4499 5647 5892 1606
Size: 528 15 1673 677 1148 245 2120
Fragments arranged by size:
2120 1673 1148 677 528 245 15
NaeI GCC'GGC
Cuts at: 2025 2144 2025
Size: 119 6287
NarI GG'CG_CC
Cuts at: 2027 2256 2027
Size: 229 6177
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4915
Fragments arranged by size:
4915 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6406
NgoMIV G'CCGG_C
Cuts at: 2023 2142 2023
Size: 119 6287
NheI G'CTAG_C
Cuts at: 1052 2629 1052
Size: 1577 4829
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 3623 3764 5309 1132
Size: 97 2394 141 1545 2229
Fragments arranged by size:
2394 2229 1545 141 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6406
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6406
PfoI T'CCnGG_A
Cuts at: 2361 2862 2361
Size: 501 5905
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 5988 6020 4475
Size: 32 1481 32 4861
Fragments arranged by size:
4861 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2359 2860 2359
Size: 501 5905
PshAI GACnn'nnGTC
Cuts at: 1613 3426 1613
Size: 1813 4593
PsiI TTA'TAA
Cuts at: 3976 3976
Size: 6406
PspOMI G'GGCC_C
Cuts at: 3579 3587 3579
Size: 8 6398
PspXI vC'TCGA_Gb
Cuts at: 2820 2820
Size: 6406
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3338 3370 3338
Size: 32 6374
PstI C_TGCA'G
Cuts at: 830 2253 3374 3758 830
Size: 1423 1121 384 3478
Fragments arranged by size:
3478 1423 1121 384
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
PvuII CAG'CTG
Cuts at: 2149 2149
Size: 6406
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 2826 3673 4654 6366 2826
Size: 847 981 1712 2866
Fragments arranged by size:
2866 1712 981 847
SfcI C'TryA_G
Cuts at: 826 1045 2249 2850 3370 3754 4889 5570 5761
Size: 219 1204 601 520 384 1135 681 191
Cuts at: 5761 826
Size: 1471
Fragments arranged by size:
1471 1204 1135 681 601 520 384 219
191
SfoI GGC'GCC
Cuts at: 2028 2257 2028
Size: 229 6177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 3733 1872
Size: 1861 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2342 2820 3088 4510 5411 5673
Size: 197 926 399 478 268 1422 901 262
Cuts at: 5673 820
Size: 1553
Fragments arranged by size:
1553 1422 926 901 478 399 268 262
197
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6406
StyI C'CwwG_G
Cuts at: 513 1065 2004 2355 3099 3575 3583 3845 513
Size: 552 939 351 744 476 8 262 3074
Fragments arranged by size:
3074 939 744 552 476 351 262 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2080 4433 4450 4603 4788 5233 325
Size: 1255 500 2353 17 153 185 445 1498
Fragments arranged by size:
2353 1498 1255 500 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2491 2795 2824
Size: 274 80 33 51 1443 514 304 29
Cuts at: 2824 2904 3671 4652 6364 96
Size: 80 767 981 1712 138
Fragments arranged by size:
1712 1443 981 767 514 304 274 138
80 80 51 33 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6366 6398 6366
Size: 32 6374
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6406
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 2820 1943
Size: 877 5529
XmaI C'CCGG_G
Cuts at: 1870 3731 1870
Size: 1861 4545
XmnI GAAnn'nnTTC
Cuts at: 4535 4535
Size: 6406
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI