Restriction map for
pFN21AE3243
(Circular) (Six-base) MAPSORT of: pFN21AE3243.seq Check: 9792 from: 1 to: 6403
pFN21AE3243 6403 bp
With 182 enzymes: SgfI *
March 28, 2012 14:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4213 278
Size: 53 83 186 936 2677 2468
Fragments arranged by size:
2677 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3746 1937
Size: 1809 4594
Acc65I G'GTAC_C
Cuts at: 3724 3724
Size: 6403
AclI AA'CG_TT
Cuts at: 1563 4530 4903 1563
Size: 2967 373 3063
Fragments arranged by size:
3063 2967 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2798 3509 4466 5850 6118 1632
Size: 1166 711 957 1384 268 1917
Fragments arranged by size:
1917 1384 1166 957 711 268
AflII C'TTAA_G
Cuts at: 820 1017 2429 820
Size: 197 1412 4794
Fragments arranged by size:
4794 1412 197
AflIII A'CryG_T
Cuts at: 1203 5302 6279 1203
Size: 4099 977 1327
Fragments arranged by size:
4099 1327 977
AgeI A'CCGG_T
Cuts at: 5234 5234
Size: 6403
AhdI GACnn_n'nnGTC
Cuts at: 2449 5132 2449
Size: 2683 3720
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6403
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6403
AlwNI CAG_nnn'CTG
Cuts at: 2692 2734 3239 4146 5718 2692
Size: 42 505 907 1572 3377
Fragments arranged by size:
3377 1572 907 505 42
ApaLI G'TGCA_C
Cuts at: 3602 4460 5616 3602
Size: 858 1156 4389
Fragments arranged by size:
4389 1156 858
ApoI r'AATT_y
Cuts at: 1493 2767 3712 3938 4090 6199 1493
Size: 1274 945 226 152 2109 1697
Fragments arranged by size:
2109 1697 1274 945 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4957 160
Size: 872 3925 1606
Fragments arranged by size:
3925 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6403
AvaI C'yCGr_G
Cuts at: 1870 1943 2758 3495 3728 1870
Size: 73 815 737 233 4545
Fragments arranged by size:
4545 815 737 233 73
BamHI G'GATC_C
Cuts at: 3733 4100 3733
Size: 367 6036
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2239 2798 3635 3724 5179
Size: 325 217 627 452 559 837 89 1455
Cuts at: 5179 618
Size: 1842
Fragments arranged by size:
1842 1455 837 627 559 452 325 217
89
BanII G_rGCy'C
Cuts at: 1391 3722 1391
Size: 2331 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3428 928
Size: 971 1529 3903
Fragments arranged by size:
3903 1529 971
BbvCI CC'TCA_GC
Cuts at: 2100 2696 3018 2100
Size: 596 322 5485
Fragments arranged by size:
5485 596 322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3506 3540 4594 4628 1903
Size: 34 1569 34 1054 34 3678
Fragments arranged by size:
3678 1569 1054 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3087 4295 5505 3087
Size: 1208 1210 3985
Fragments arranged by size:
3985 1210 1208
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6403
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5014 136
Size: 107 122 71 631 605 3342 1525
Fragments arranged by size:
3342 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6398 6398
Size: 6403
BlpI GC'TnA_GC
Cuts at: 3820 3820
Size: 6403
Bme1580I G_kGCm'C
Cuts at: 1790 3606 4464 5620 1790
Size: 1816 858 1156 2573
Fragments arranged by size:
2573 1816 1156 858
BmgBI CAC'GTC
Cuts at: 1318 2176 1318
Size: 858 5545
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2559 2697 4134
Size: 416 53 83 399 24 1142 138 1437
Cuts at: 4134 5092 442
Size: 958 1753
Fragments arranged by size:
1753 1437 1142 958 416 399 138 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2747 1056
Size: 1691 4712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2756 2778 3230 5063 1239
Size: 1517 22 452 1833 2579
Fragments arranged by size:
2579 1833 1517 452 22
Bpu10I CC'TnA_GC
Cuts at: 2100 2696 3018 2100
Size: 596 322 5485
Fragments arranged by size:
5485 596 322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4528 5393 5691 4528
Size: 865 298 5240
Fragments arranged by size:
5240 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3675 5066 882
Size: 617 2176 1391 2219
Fragments arranged by size:
2219 2176 1391 617
BsaAI yAC'GTr
Cuts at: 493 1204 3601 4194 493
Size: 711 2397 593 2702
Fragments arranged by size:
2702 2397 711 593
BsaBI GATnn'nnATC
Cuts at: 6397 6397
Size: 6403
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4210 4592 5227 275
Size: 53 83 186 936 2677 382 635 1451
Fragments arranged by size:
2677 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4836 5234 5508 5655 6066 4836
Size: 398 274 147 411 5173
Fragments arranged by size:
5173 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2271 2301 2437 2467 3059
Size: 30 165 30 868 30 136 30 592
Cuts at: 3059 3089 1178
Size: 30 4492
Fragments arranged by size:
4492 868 592 165 136 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3080 3278 3626 1186
Size: 1894 198 348 3963
Fragments arranged by size:
3963 1894 348 198
BseYI C'CCAG_C
Cuts at: 2071 3326 5606 6102 2071
Size: 1255 2280 496 2372
Fragments arranged by size:
2372 2280 1255 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3169 3238 1163
Size: 573 1433 69 4328
Fragments arranged by size:
4328 1433 573 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3853 4614 5642 1540
Size: 275 186 1852 761 1028 2301
Fragments arranged by size:
2301 1852 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3606 4464 4549 5620 3606
Size: 858 85 1071 4389
Fragments arranged by size:
4389 1071 858 85
BsmI GAATG_Cn'
Cuts at: 2956 3914 4007 2956
Size: 958 93 5352
Fragments arranged by size:
5352 958 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3606 3722 4464 4549 5620 1391
Size: 399 1816 116 742 85 1071 2174
Fragments arranged by size:
2174 1816 1071 742 399 116 85
BspEI T'CCGG_A
Cuts at: 6066 6066
Size: 6403
BspHI T'CATG_A
Cuts at: 4292 6022 4292
Size: 1730 4673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3434 3693 3758 844
Size: 2590 259 65 3489
Fragments arranged by size:
3489 2590 259 65
BsrBI CCG'CTC
Cuts at: 1394 1823 4290 1394
Size: 429 2467 3507
Fragments arranged by size:
3507 2467 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3394 4898 5072 65
Size: 1183 2146 1504 174 1396
Fragments arranged by size:
2146 1504 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2416 2934 5047 5234 1700
Size: 110 606 518 2113 187 2869
Fragments arranged by size:
2869 2113 606 518 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6403
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6403
BssSI C'ACGA_G
Cuts at: 3525 4463 5475 3525
Size: 938 1012 4453
Fragments arranged by size:
4453 1012 938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6403
BstBI TT'CG_AA
Cuts at: 5207 5207
Size: 6403
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6403
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 3112 2062
Size: 1050 5353
BstYI r'GATC_y
Cuts at: 1972 3693 3733 4100 4485 4502 5954 6040 6052
Size: 1721 40 367 385 17 1452 86 12
Cuts at: 6052 6398 1972
Size: 346 1977
Fragments arranged by size:
1977 1721 1452 385 367 346 86 40
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2094 2787 513
Size: 552 939 90 693 4129
Fragments arranged by size:
4129 939 693 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5664
Fragments arranged by size:
5664 683 56
BtsI GCAGTG_nn'
Cuts at: 3604 3919 4713 4733 3604
Size: 315 794 20 5274
Fragments arranged by size:
5274 794 315 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6403
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6368
DraI TTT'AAA
Cuts at: 3707 3707
Size: 6403
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5410 809
Size: 507 4094 1802
Fragments arranged by size:
4094 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2418 3850 4739
Size: 54 1425 50 275 18 588 1432 889
Cuts at: 4739 6368 8
Size: 1629 43
Fragments arranged by size:
1629 1432 1425 889 588 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3850 1537
Size: 275 2038 4090
Fragments arranged by size:
4090 2038 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4333 1353
Size: 2980 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4990 5364 5510 387
Size: 1064 138 3401 374 146 1280
Fragments arranged by size:
3401 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2756 2778 2798 3230 3509 4466 5063
Size: 393 1124 22 20 432 279 957 597
Cuts at: 5063 5850 6118 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1124 957 787 597 432 393 279
268 22 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2777 2777
Size: 6403
EcoO109I rG'GnC_Cy
Cuts at: 2665 2689 2665
Size: 24 6379
EcoRI G'AATT_C
Cuts at: 2767 3712 4090 2767
Size: 945 378 5080
Fragments arranged by size:
5080 945 378
FspI TGC'GCA
Cuts at: 4909 4909
Size: 6403
HaeII r_GCGC'y
Cuts at: 1678 3677 5550 1678
Size: 1999 1873 2531
Fragments arranged by size:
2531 1999 1873
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2435 2467 3362
Size: 32 135 32 455 32 376 32 895
Cuts at: 3362 3394 3551 3583 5044 5076 5118 5150 1373
Size: 32 157 32 1461 32 42 32 2626
Fragments arranged by size:
2626 1461 895 455 376 157 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2167 3747 3993 669
Size: 988 281 229 1580 246 3079
Fragments arranged by size:
3079 1580 988 281 246 229
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6403
HpaI GTT'AAC
Cuts at: 3993 3993
Size: 6403
KpnI G_GTAC'C
Cuts at: 3728 3728
Size: 6403
MfeI C'AATT_G
Cuts at: 4002 4002
Size: 6403
MluI A'CGCG_T
Cuts at: 6279 6279
Size: 6403
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2876 5517 5701 1305
Size: 1571 2641 184 2007
Fragments arranged by size:
2641 2007 1571 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4581
Fragments arranged by size:
4581 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2349 3554 4361 4720 4879 518
Size: 648 1183 1205 807 359 159 2042
Fragments arranged by size:
2042 1205 1183 807 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2509 2645 3153 3467 3819 4496 5644 5889
Size: 903 136 508 314 352 677 1148 245
Cuts at: 5889 1606
Size: 2120
Fragments arranged by size:
2120 1148 903 677 508 352 314 245
136
NaeI GCC'GGC
Cuts at: 2418 2418
Size: 6403
NcoI C'CATG_G
Cuts at: 513 1065 2004 2787 513
Size: 552 939 783 4129
Fragments arranged by size:
4129 939 783 552
NdeI CA'TA_TG
Cuts at: 387 2721 387
Size: 2334 4069
NgoMIV G'CCGG_C
Cuts at: 2416 2416
Size: 6403
NheI G'CTAG_C
Cuts at: 1052 2743 1052
Size: 1691 4712
NotI GC'GGCC_GC
Cuts at: 3850 3850
Size: 6403
NspI r_CATG'y
Cuts at: 1132 1229 3761 5306 1132
Size: 97 2532 1545 2229
Fragments arranged by size:
2532 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3631 1348
Size: 2283 4120
PciI A'CATG_T
Cuts at: 5302 5302
Size: 6403
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6403
PfoI T'CCnGG_A
Cuts at: 3111 3111
Size: 6403
PmeI GTTT'AAAC
Cuts at: 3707 3707
Size: 6403
PmlI CAC'GTG
Cuts at: 3601 3601
Size: 6403
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4472 4504 5985 6017 4472
Size: 32 1481 32 4858
Fragments arranged by size:
4858 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2689 2689
Size: 6403
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6403
PsiI TTA'TAA
Cuts at: 3973 3973
Size: 6403
PspXI vC'TCGA_Gb
Cuts at: 2758 2758
Size: 6403
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2030 2062 2030
Size: 32 6371
PstI C_TGCA'G
Cuts at: 830 2715 3755 830
Size: 1885 1040 3478
Fragments arranged by size:
3478 1885 1040
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6403
PvuII CAG'CTG
Cuts at: 2509 3467 2509
Size: 958 5445
SalI G'TCGA_C
Cuts at: 1936 3745 1936
Size: 1809 4594
SbfI CC_TGCA'GG
Cuts at: 3755 3755
Size: 6403
ScaI AGT'ACT
Cuts at: 4651 6363 4651
Size: 1712 4691
SfcI C'TryA_G
Cuts at: 826 1045 2711 3751 4886 5567 5758 826
Size: 219 1666 1040 1135 681 191 1471
Fragments arranged by size:
1666 1471 1135 1040 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6403
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6403
SmaI CCC'GGG
Cuts at: 1872 3730 1872
Size: 1858 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2429 2758 4507 5408 5670 820
Size: 197 926 486 329 1749 901 262 1553
Fragments arranged by size:
1749 1553 926 901 486 329 262 197
SnaBI TAC'GTA
Cuts at: 493 4194 493
Size: 3701 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6403
SphI G_CATG'C
Cuts at: 1132 3761 1132
Size: 2629 3774
SspI AAT'ATT
Cuts at: 5 52 4327 5
Size: 47 4275 2081
Fragments arranged by size:
4275 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6403
StyI C'CwwG_G
Cuts at: 513 1065 2004 2787 3279 3842 513
Size: 552 939 783 492 563 3074
Fragments arranged by size:
3074 939 783 563 552 492
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3623 4430 4447 4600 4785 5230 325
Size: 1255 2043 807 17 153 185 445 1498
Fragments arranged by size:
2043 1498 1255 807 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4649 6361 96
Size: 274 80 33 51 1443 2672 1712 138
Fragments arranged by size:
2672 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2067 2099 2437 2469 3059 3091 3619 3651 6363
Size: 32 338 32 590 32 528 32 2712
Cuts at: 6363 6395 2067
Size: 32 2075
Fragments arranged by size:
2712 2075 590 528 338 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6403
XbaI T'CTAG_A
Cuts at: 2314 3739 2314
Size: 1425 4978
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2231 3109 2231
Size: 878 5525
XhoI C'TCGA_G
Cuts at: 1943 2758 1943
Size: 815 5588
XmaI C'CCGG_G
Cuts at: 1870 3728 1870
Size: 1858 4545
XmnI GAAnn'nnTTC
Cuts at: 4532 4532
Size: 6403
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4211 276
Size: 53 83 186 936 2677 2468
Fragments arranged by size:
2677 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PspOMI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI